CN104719206A - Holothuria scabra artificial seeding breeding method - Google Patents
Holothuria scabra artificial seeding breeding method Download PDFInfo
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- 241000133638 Holothuria scabra Species 0.000 title claims abstract description 35
- 238000009395 breeding Methods 0.000 title claims abstract description 13
- 238000010899 nucleation Methods 0.000 title abstract 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 54
- 239000000843 powder Substances 0.000 claims abstract description 38
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 25
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 25
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 25
- 229940082787 spirulina Drugs 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
- 241000208340 Araliaceae Species 0.000 claims description 33
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 33
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 33
- 235000008434 ginseng Nutrition 0.000 claims description 33
- 241000593522 Sargassum thunbergii Species 0.000 claims description 19
- 230000003203 everyday effect Effects 0.000 claims description 17
- 230000001418 larval effect Effects 0.000 claims description 12
- 230000013011 mating Effects 0.000 claims description 11
- 239000002689 soil Substances 0.000 claims description 10
- 230000002354 daily effect Effects 0.000 claims description 9
- 230000029052 metamorphosis Effects 0.000 claims description 9
- 239000013535 sea water Substances 0.000 claims description 9
- 241000965254 Apostichopus japonicus Species 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 230000000474 nursing effect Effects 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 210000004681 ovum Anatomy 0.000 claims description 4
- 230000001902 propagating effect Effects 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 2
- 241000251511 Holothuroidea Species 0.000 abstract description 8
- 241000195493 Cryptophyta Species 0.000 abstract description 5
- 238000004113 cell culture Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 238000007747 plating Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 241001412627 Adenophora liliifolia Species 0.000 description 1
- 241000091751 Chaetoceros muellerii Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 240000007058 Halophila ovalis Species 0.000 description 1
- 241000258196 Holothuriidae Species 0.000 description 1
- 241001501873 Isochrysis galbana Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000009798 Shen-Fu Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005183 environmental health Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000012286 potassium permanganate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Fodder In General (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention provides a holothuria scabra artificial seeding breeding method. The method comprises the steps such as parent holothurian cultivating, induced spawning, spawn washing and hatching, larva cultivating, seedling collecting and childish holothurian cultivating, wherein spirulina powder, marine yeast, aquatic yeast and bacillus serve as bait, not only are bait sources convenient, but also the investment of single cell culture facilities is saved, meanwhile, manpower needed by single cell algae bait culture is saved, the holothurian seedling cost can be greatly saved, and the popularization of the holothuria scabra seedling breeding technology is facilitated. The substitutive bait is adopted to replace living single cell algae, invested funds of seeding facilities can be saved by about 25 percents, the human input of seeding can be saved by about 30 percents, and the ratio of the seeding input to the seeding output 1:3. Induced spawning is performed on the two days before or after the first day and the fifteenth day of a lunar month, the good effect of two times of spawn sampling in one month is achieved, the induced spawning success rate is 83.3 percents, and the technical problem that the induced spawning difficulty of holothuria scabra parent holothurian is high is effectively solved.
Description
Technical field
The invention belongs to aquaculture field, relate to the mating system of a kind of Holothuria scabra artificial seed particularly.
Background technology
Holothuria scabra, formal name used at school: Holothuria scabra, it is the animal that Holothuriidae sea cucumber belongs to, popular name bright beautiful ginseng, the root of straight ladybell, Bai Can, it is a kind of beche-de-mer of high-quality, having the feature of high protein, low fat, low sugar, have very high nutritive value, economic worth and health care, is real environmental health food.In the world, Holothuria scabra is also the high-grade aquatic products of a kind of important tropical sea cucumber breed variety and foreign exchange earning.Be distributed in west from Natta ear port to Red sea, eastwards to Caroline, Fiji Islands, northwards to Japan, southwards to the ground such as Guangxi, Guangdong, Hainan of Australia and China's Mainland, generally move in fringing reef edge and tidewater flow velocity is fast and sea grass the is many husky end.As the link that holothruian cultures are important, breading sea cucumber seedlings technology is generally launched in China's Coastal Areas.
But mainly there are following two aspect problems in current Holothuria scabra seed breeding: one, the young stage of swimming adopts Chaetoceros muelleri, Isochrysis galbana, P latymonas subcordifomis and chlorella to be bait, because Holothuria scabra nursery southern area in season water temperature is higher, above culture of living feeds is easy to be subject to protozoa and pollutes and cause failure, thus causes the supply of sea cucumber young bait to be difficult to ensure; Two, effective ways, Holothuria scabra egg-laying pattern and effective abductive approach that Holothuria scabra parent participation is cultivated is not explored.
Summary of the invention
The object of the invention is to overcome deficiency of the prior art, provide the mating system of a kind of Holothuria scabra artificial seed, cost is low, and induced spawning rate is high, and the seed output and quality of Holothuria scabra seed is good high.
First aspect of the present invention is to provide the mating system of a kind of Holothuria scabra artificial seed, comprises the following steps:
(1) parent participation is cultivated:
Selecting the natural Holothuria scabra of body weight 150-400g or propagating Holothuria scabra artificially is parent participation, puts in a suitable place to breed and cultivates in indoor water mud sump, breeding density 10-15 head/m
2, at the bottom of pond, place things concealed; Bait throwing at dusk 1 time every day, daily ration of feeding is the 3-5% of parent participation body weight, wherein, according to weight parts, the nursing bait of parent participation comprises sargassum thunbergii powder 400-450 part, stichopus japonicus parent participation manufactured feed 400-450 part, ooze 100-200 part, aquatic products yeast 3-8 part and B B-complex 3-8 part; Continuous flowing water and inflation, maintenance day streamflow is for cultivating water body 3-5 doubly, seawater salinity 25-32, more than dissolved oxygen content 5mg/L; At the bottom of regular removing pond at the bottom of residual bait, flushing tank and change water;
(2) induced spawning:
In the spawning season in Holothuria scabra 3-10 month, select lunar calendar junior one every month and/or 15 front and back carry out induced spawning in 2 days, induced spawning stops bait throwing in first 1 day, the that afternoon of induced spawning, select healthy and strong parent participation, with filtering sea, ginseng surface is rinsed well, then be put in indoor raising of seedling pond and reveal sky and to dry in the shade 30-60min, then use filtering sea stimulation by running water 30-60min, reinject seawater, treats Spawning;
(3) ovum and hatching is washed:
After Spawning terminates, pull parent participation out, ight soil is removed in siphon, and change water and remove unnecessary sperm, each quantity of exchanged water 40-60% 1-2 time, fertilized egg is hatched in former induced spawning pond;
(4) larval culture:
Incubating oosperm is grown for after the first ear young, calculates young density, and controlling just ear young breeding density is 0.1-0.3/mL; With spirulina powder, marine yeast, aquatic products yeast and bacillus for bait, bait throwing in 3-4 time every day, each bait throwing in concentration spirulina powder 0.03-0.10mg/L, marine yeast 0.1-0.15mg/L, aquatic products yeast 0.03-0.10mg/L, bacillus 0.05-0.08mg/L; The young puts the 1st to the 3rd day in a suitable place to breed, adds the 8-15% sand filter seawater of seedling water every day, and after the 4th day, every day changes water 20-40%; It is doliolaria that the young cultivated growth metamorphosis through 8-11 days;
(5) collect seedling:
When the large ear larval metamorphosis of 25-35% is doliolaria, throw in collector complete to doliolaria attachment, every day period changes water 40-60%;
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis is in 10 days after young ginseng, except spirulina of throwing something and feeding, marine yeast, aquatic products yeast and bacillus, starts throw something and feed young ginseng manufactured feed, sargassum thunbergii powder and ooze.Each daily ration, feeding quantity is spirulina powder 0.04-0.06mg/L, marine yeast 0.04-0.06mg/L, aquatic products yeast 0.03-0.05mg/L, bacillus 0.02-0.04mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L, morning and at dusk each throwing 1 time;
Young Shenfu latter 11st to the 30th day, stops throwing spirulina, marine yeast and aquatic products yeast, and each bait throwing in concentration is bacillus 0.3-1.0mg/L, young ginseng manufactured feed 1.5-2.5mg/L, sargassum thunbergii powder 2.5-4.0mg/L, ooze 4.0-7.0mg/L, morning and at dusk each throwing 1 time; Cultivate under miniflow water and continuous charge condition, day streamflow be seedling water 0.5-1 doubly, more than dissolved oxygen 5mg/L; Regularly residual bait and ight soil at the bottom of the removing of falling pond pond; When young ginseng growth reaches the long 09-1.1cm of body, young ginseng is peeled off and continues to cultivate at the bottom of pond; When young ginseng grows to more than 2cm, can be used for pond net box and cultivate or cultivation.
Further, in step (1), in the nursing bait of parent participation, sargassum thunbergii powder, stichopus japonicus parent participation manufactured feed and ooze three proportioning are 4.5: 4.5: 1.
Further, during the young ginseng of step (6) is cultivated, each daily ration, feeding quantity is spirulina powder 0.05mg/L, marine yeast 0.05mg/L, aquatic products yeast 0.04mg/L, bacillus 0.03mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L.
Further, in step (1), regularly at the bottom of removing pond at the bottom of residual bait, flushing tank and the frequency of changing water be: siphon in every 1-3 days removes residual bait at the bottom of ponds 1 time, at the bottom of every 6-8 days Drain rinse ponds 1 time, and every 10-20 days falls pond 1 time.
Further, in step (6), regularly the frequency of residual bait at the bottom of the removing of falling pond pond and ight soil is: within 7-10 days, fall pond and remove residual bait and ight soil 1 time.
Further, in step (5), collector adopts plastic corrugated sheet.
Second aspect of the present invention is to provide a kind of Holothuria scabra larval culture bait, and according to weight parts, described bait comprises spirulina powder 0.03-0.10 part, marine yeast 0.1-0.15 part, aquatic products yeast 0.03-0.10 part, bacillus 0.05-0.08 part.
3rd aspect of the present invention is to provide the using method of the Holothuria scabra larval culture bait described in the present invention second aspect, controlling just ear young breeding density is 0.1-0.3/mL, bait throwing in 3-4 time every day, each bait throwing in concentration is: spirulina powder 0.03-0.10mg/L, marine yeast 0.1-0.15mg/L, aquatic products yeast 0.03-0.10mg/L, bacillus 0.05-0.08mg/L.
The present invention adopts spirulina powder, marine yeast, aquatic products yeast, bacillus etc. to replace living body biological bait, not only feed source is convenient, and save the input that unicellular class cultivates facility, save the manpower required for the cultivation of Micro Algae bait simultaneously, can significantly save breading sea cucumber seedlings cost, and be conducive to the popularization of Holothuria scabra seed breeding technology.Adopt substitute bait to replace Living single cell algae, can save culture facility infusion of financial resources about 25%, can save nursery human input about 30%, nursery input and output is than 1: 3.The substitute bait such as visible employing spirulina powder, marine yeast, food yeast, bacillus replaces Living single cell algae as Holothuria scabra young bait, effectively ensures the supply of young bait.
The present invention also explores mating season and the egg-laying pattern of Holothuria scabra, before and after the selection lunar calendar junior one and 15, in 2 days, induced spawning obtains the good achievement of adopting ovum for 1 month 2 times, induced spawning success rate is 83.3%, has and solves the high technical barrier of Holothuria scabra parent participation induced spawning difficulty.
The present invention adopts above Holothuria scabra nursery new technology to have very high using value, and easily promotes.
Embodiment
Referring to specific embodiment, the present invention is described further, to understand the present invention better.
Embodiment 1
(1) parent participation is cultivated:
Selecting the natural Holothuria scabra of body weight 150-400g or propagating Holothuria scabra artificially is parent participation, puts in a suitable place to breed in 10-20m
3cultivate in indoor water mud sump, breeding density 10-15 head/m
2.At the bottom of pond, placement 25cm × 25cm, pin height 5cm cement four pin brick are things concealed.Throw something and feed with mixing such as sargassum thunbergii powder, stichopus japonicus parent participation manufactured feed, aquatic products yeast, ooze and B B-complexes, bait throwing at dusk 1 time every day, daily ration of feeding is the 3-5% of body weight.According to weight parts, the nursing bait of parent participation comprises sargassum thunbergii powder 400-450 part, stichopus japonicus parent participation manufactured feed 400-450 part, ooze 100-200 part, aquatic products yeast 3-8 part (general employing 5 parts) and B B-complex 3-8 part (general employing 5 parts).General sargassum thunbergii powder, stichopus japonicus parent participation manufactured feed and ooze three proportioning are 4.5: 4.5: 1.
Siphon in every 2 days removes residual bait at the bottom of pond 1 time, at the bottom of every 7 days Drain rinse ponds 1 time, and every two weeks falls pond 1 time.Continuous flowing water and inflation.Day, streamflow was for cultivating water body 3-5 doubly, seawater salinity 25-32, more than dissolved oxygen content 5mg/L.Parent participation cultivates 10 months, survival rate 95.8%.
(2) induced spawning method:
In the spawning season in Holothuria scabra 3-10 month, select lunar calendar junior one every month and 15 front and back carry out induced spawning in 2 days, induced spawning stops bait throwing in first 1 day.That afternoon of induced spawning, select healthy and strong parent participation, with filtering sea, ginseng surface is rinsed well, be then put in indoor raising of seedling pond and reveal sky and to dry in the shade 30-60min, then use filtering sea stimulation by running water 30-60min, the 80cm seawater that reinjects waits for Spawning.
Carry out 12 parent participation induced spawnings altogether, wherein, 10 times induced spawning succeeds, and induced spawning success rate is 83.3%, altogether the hatching just ear young 2584.4 ten thousand.
(3) ovum and hatching is washed:
After Spawning terminates, pull parent participation out, ight soil is removed in siphon, and change water and remove unnecessary sperm 1-2 time, each quantity of exchanged water 50%, fertilized egg is hatched in former induced spawning pond.
(4) larval culture:
Incubating oosperm is grown for after the first ear young, calculates young density, as young density be greater than 0.3/mL time, young siphon is collected in 300 order net cages, then the young is divided and be negligent of cultivating in other nursery ponds.First ear young breeding density is 0.1-0.3/mL.With spirulina powder, marine yeast, aquatic products yeast and bacillus for bait, bait throwing in 3-4 time every day, each bait throwing in concentration spirulina powder 0.03-0.10mg/L, marine yeast 0.1-0.15mg/L, aquatic products yeast 0.03-0.10mg/L, bacillus 0.05-0.08mg/L.The young puts the 1st to the 3rd day in a suitable place to breed, adds 10% sand filter seawater of seedling water every day; After 4th day, every day changes water 30%.It is doliolaria that the young cultivated growth metamorphosis through 8-11 days.
Carry out 10 batches of larvae cultivations altogether, wherein, 8 batches of larval cultures obtain successfully (wherein, twice larval culture is unsuccessfully because of caused by typhoon influence).
(5) collect seedling:
Collecting seedling by specification is 40cm × 30cm polyethylene corrugated plate, and corrugated plating is first with 20mg/L concentration potassium permanganate sterilization 20min, and then clean with fresh water flush, every 10 corrugated platings are put into plastic crate frame and formed a bundle, for subsequent use after airing.When 30% large ear larval metamorphosis is doliolaria, the plastic corrugated sheet becoming to prick is placed horizontally in nursery pond.Again the plastic corrugated sheet becoming to prick vertically is placed after young Shenfu the 10th day.Throw in collector to doliolaria attachment complete period, change water 50% every day.
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis is in latter 10 days of young ginseng, except spirulina of throwing something and feeding, marine yeast, outside aquatic products yeast and bacillus, start young ginseng manufactured feed of throwing something and feeding, sargassum thunbergii powder and ooze, each daily ration, feeding quantity is spirulina powder 0.04-0.06mg/L, marine yeast 0.04-0.06mg/L, aquatic products yeast 0.03-0.05mg/L, bacillus 0.02-0.04mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L (such as: spirulina powder 0.05mg/L, marine yeast 0.05mg/L, aquatic products yeast 0.04mg/L, bacillus 0.03mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L), morning and at dusk each throwing 1 time.
Young Shenfu latter 11st to the 30th day, stops throwing spirulina, marine yeast and aquatic products yeast, and each bait throwing in concentration is bacillus 0.3-1.0mg/L, young ginseng manufactured feed 1.5-2.5mg/L, sargassum thunbergii powder 2.5-4.0mg/L, ooze 4.0-7.0mg/L.Morning and at dusk each throwing 1 time.Cultivate under miniflow water and continuous charge condition, day streamflow be seedling water 0.5-1 doubly, more than dissolved oxygen 5mg/L.Within 7-10 days, fall pond and remove residual bait and ight soil 1 time.When young ginseng growth reaches the long 1cm of body, peel off continue to cultivate at the bottom of pond being attached to the young ginseng of corrugated plating.When young ginseng grows to more than 2cm, can be used for pond net box and cultivate or cultivation.
The long 2-5.5cm seedling of nurture body 88.1 ten thousand altogether.
The present invention can save culture facility infusion of financial resources 25%, and can save nursery human input 30%, nursery input and output is than 1: 3.
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (8)
1. a Holothuria scabra artificial seed's mating system, is characterized in that, comprises the following steps:
(1) parent participation is cultivated:
Selecting the natural Holothuria scabra of body weight 150-400g or propagating Holothuria scabra artificially is parent participation, puts in a suitable place to breed and cultivates in indoor water mud sump, breeding density 10-15 head/m
2, at the bottom of pond, place things concealed; Bait throwing at dusk 1 time every day, daily ration of feeding is the 3-5% of parent participation body weight, wherein, according to weight parts, the nursing bait of parent participation comprises sargassum thunbergii powder 400-450 part, stichopus japonicus parent participation manufactured feed 400-450 part, ooze 100-200 part, aquatic products yeast 3-8 part and B B-complex 3-8 part; Continuous flowing water and inflation, maintenance day streamflow is for cultivating water body 3-5 doubly, seawater salinity 25-32, more than dissolved oxygen content 5mg/L; At the bottom of regular removing pond at the bottom of residual bait, flushing tank and change water;
(2) induced spawning:
In the spawning season in Holothuria scabra 3-10 month, select lunar calendar junior one every month and/or 15 front and back carry out induced spawning in 2 days, induced spawning stops bait throwing in first 1 day, the that afternoon of induced spawning, select healthy and strong parent participation, with filtering sea, ginseng surface is rinsed well, then be put in indoor raising of seedling pond and reveal sky and to dry in the shade 30-60min, then use filtering sea stimulation by running water 30-60min, reinject seawater, treats Spawning;
(3) ovum and hatching is washed:
After Spawning terminates, pull parent participation out, ight soil is removed in siphon, and change water and remove unnecessary sperm, each quantity of exchanged water 40-60% 1-2 time, fertilized egg is hatched in former induced spawning pond;
(4) larval culture:
Incubating oosperm is grown for after the first ear young, calculates young density, and controlling just ear young breeding density is 0.1-0.3/mL; With spirulina powder, marine yeast, aquatic products yeast and bacillus for bait.Bait throwing in 3-4 time every day, each bait throwing in concentration spirulina powder 0.03-0.10mg/L, marine yeast 0.1-0.15mg/L, aquatic products yeast 0.03-0.10mg/L, bacillus 0.05-0.08mg/L; The young puts the 1st to the 3rd day in a suitable place to breed, adds the 8-15% sand filter seawater of seedling water every day, and after the 4th day, every day changes water 20-40%; It is doliolaria that the young cultivated growth metamorphosis through 8-11 days;
(5) collect seedling:
When the large ear larval metamorphosis of 25-35% is doliolaria, throw in collector complete to doliolaria attachment, every day period changes water 40-60%;
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis is in 10 days after young ginseng, except spirulina of throwing something and feeding, marine yeast, aquatic products yeast and bacillus, starts throw something and feed young ginseng manufactured feed, sargassum thunbergii powder and ooze.Each daily ration, feeding quantity is spirulina powder 0.04-0.06mg/L, marine yeast 0.04-0.06mg/L, aquatic products yeast 0.03-0.05mg/L, bacillus 0.02-0.04mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L, morning and at dusk each throwing 1 time;
Young Shenfu latter 11st to the 30th day, stops throwing spirulina, marine yeast and aquatic products yeast, and each daily ration, feeding quantity is bacillus 0.3-1.0mg/L, young ginseng manufactured feed 1.5-2.5mg/L, sargassum thunbergii powder 2.5-4.0mg/L, ooze 4.0-7.0mg/L, morning and at dusk each throwing 1 time; Cultivate under miniflow water and continuous charge condition, day streamflow be seedling water 0.5-1 doubly, more than dissolved oxygen 5mg/L; Regularly residual bait and ight soil at the bottom of the removing of falling pond pond; When young ginseng growth reaches the long 0.9-1.1cm of body, young ginseng is peeled off and continues to cultivate at the bottom of pond; When young ginseng grows to more than 2cm, can be used for pond net box and cultivate or cultivation.
2. mating system according to claim 1, is characterized in that, in step (1), in the nursing bait of parent participation, sargassum thunbergii powder, stichopus japonicus parent participation manufactured feed and ooze three proportioning are 4.5: 4.5: 1.
3. mating system according to claim 1, it is characterized in that, during the young ginseng of step (6) is cultivated, each daily ration, feeding quantity is spirulina powder 0.05mg/L, marine yeast 0.05mg/L, aquatic products yeast 0.04mg/L, bacillus 0.03mg/L, young ginseng manufactured feed 0.6-1.2mg/L, sargassum thunbergii powder 0.9-1.8mg/L, ooze 1.5-3.0mg/L.
4. mating system according to claim 1, it is characterized in that, in step (1), regularly at the bottom of removing pond at the bottom of residual bait, flushing tank and the frequency of changing water be: siphon in every 1-3 days removes residual bait at the bottom of ponds 1 time, at the bottom of every 6-8 days Drain rinse ponds 1 time, every 10-20 days falls pond 1 time.
5. mating system according to claim 1, is characterized in that, in step (6), regularly the frequency of residual bait at the bottom of the removing of falling pond pond and ight soil is: within 7-10 days, fall pond and remove residual bait and ight soil 1 time.
6. mating system according to claim 1, is characterized in that, in step (5), collector adopts plastic corrugated sheet.
7. a Holothuria scabra larval culture bait, is characterized in that, according to weight parts, described bait comprises spirulina powder 0.03-0.10 part, marine yeast 0.1-0.15 part, aquatic products yeast 0.03-0.10 part, bacillus 0.05-0.08 part.
8. the using method of a Holothuria scabra larval culture bait according to claim 7, it is characterized in that, controlling just ear young breeding density is 0.1-0.3/mL, bait throwing in 3-4 time every day, each bait throwing in concentration is: spirulina powder 0.03-0.10mg/L, marine yeast 0.1-0.15mg/L, aquatic products yeast 0.03-0.10mg/L, bacillus 0.05-0.08mg/L.
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