CN112655610A - Efficient cultivation method for tropical sea cucumber seedlings - Google Patents
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Abstract
The invention discloses a high-efficiency cultivation method of tropical sea cucumber seedlings. The technical scheme of the invention comprises the following steps of setting reasonable cultivation density, utilizing an air pump to automatically and fully stir floating water, adopting various methods to improve the metamorphosis and attachment efficiency of larvae, reasonably feeding live algae-free baits to stabilize the water, and discloses a cultivation method of tropical sea cucumbers, wherein the cultivation method has the following beneficial effects: the air pump is utilized to automatically and fully stir the floating water body, so that the labor consumption is greatly reduced, and the survival rate of the floating larvae is improved in a more convenient and efficient mode. On the premise of ensuring higher cultivation efficiency, the method feeds the live-algae-free bait, simplifies operation, reduces energy consumption, effectively prevents the risk of plant diseases and insect pests brought by live algae, does not need to replace water body in the early stage, obviously enhances controllability, obviously improves the cultivation efficiency of tropical sea cucumbers, and provides a solid technical foundation for realizing large-scale cultivation of new varieties of tropical sea cucumbers and sustainable development and utilization of tropical sea cucumber resources.
Description
The technical field is as follows:
the invention belongs to the field of aquaculture, and particularly relates to an efficient cultivation method of tropical sea cucumber seedlings.
Background art:
sea cucumber (Holothuroidea) is one of eight rare products in the world, has important food therapy value, and is the most important consumer market in China. However, except for Apostichopus japonicus (Apostichopus japonicus), commercial artificial breeding and cultivation are realized, and other 60 kinds of edible economic sea cucumbers are obtained in a field catching mode and are mostly tropical sea cucumber kinds. With the development of economy in China, the demand of tropical sea cucumbers is rapidly increased, and in 1996 to 2011, the number of countries supplying the tropical sea cucumbers to markets in China is increased from 35 to 83, more than 90% of tropical coastline areas supply the tropical sea cucumbers to markets in China, and the price of the tropical sea cucumbers is increased at a speed of 16.6% in 2011-2016, so that the field capturing activity of the tropical sea cucumbers is further stimulated.
The characteristics of slow growth speed, long sexual maturity time and the like of the sea cucumbers determine that the supplement period of new individuals of the sea cucumber population is long, the over-fishing causes the sea cucumber population resources to be damaged, and according to estimation, about 10 ten thousand tons of wild fresh and alive sea cucumbers are fished in the world every year. The tropical sea cucumber resources are exhausted after disordered fishing in south China sea waters for many years. Aiming at the current situations that the demand of the tropical sea cucumber consumption market in China is continuously vigorous and the tropical sea cucumber resources are gradually exhausted in the world, the method develops the large-scale breeding technical customs of the tropical sea cucumbers, realizes the large-scale breeding of the tropical sea cucumbers, can effectively relieve the dilemma faced by the tropical sea cucumber resources, and is the only way for sustainable development and utilization of the tropical sea cucumber resources.
The applicant breaks through the cultivation technology of tropical holothurian in earlier stage (application patent: a method for artificially cultivating reef-dwelling holothurian seedlings in small water body, application number: 201710967254.4), obtains a small amount of juvenile holothurian of leucospilota, reports the development process of tropical holothurian for the first time, and finds that the cultivation efficiency of the juvenile holothurian of tropical holothurian is still low in the further practical process, and the main problems are that: 1. in the cultivation period of planktonic life, the manpower consumption is large by adopting a mode of manually stirring water body regularly (ZL201010604454.1 a sea cucumber seedling cultivation method; 201210083064.3 a novel stichopus japonicus seedling cultivation method), the uneven manual stirring still causes partial settlement death of seedlings, the cultivation success rate of larvae is influenced, the mortality rate of 2, the planktonic stage and the adhering and metamorphosis stage is high, according to the previous research data of the applicant, the mortality rate from the late stage of big ear to the stage of goblet development reaches 54.2%, the mortality rate from the stage of goblet development to the stage of five tentacles larvae reaches 45.52%, and the survival rate (adhesion rate) for completing metamorphosis and adhesion from the late stage of planktonic big ear is only 22.56% (Spawning, large deformation and great growth of the longitudinal section a cancer Holothuria leucoderma.
The invention content is as follows:
the invention aims to provide an efficient cultivation method of tropical sea cucumber seedlings, which can efficiently produce the tropical sea cucumber seedlings on a large scale, promote the development of the breeding industry of new breeding varieties of the tropical sea cucumbers and further provide a technical foundation for the utilization of tropical sea cucumber resources.
The efficient cultivation method of the tropical sea cucumber seedlings comprises the following steps:
A. the efficient cultivation method in the planktonic life stage comprises the following steps: putting the fertilized eggs of the tropical sea cucumbers into seawater to enable the density of the fertilized eggs in a water body of the tropical sea cucumbers to be 0.05-0.15/mL, setting the density of air chambers in the water body to be 0.5-1.0 square meter/mL, adjusting the inflation strength of the air chambers to the moving speed of air bubbles on the water surface to be 15-30 cm/s, feeding baits, rubbing and crushing the baits through a 300-500-mesh bolting silk and then feeding the baits;
B. the efficient cultivation method in the attachment metamorphosis stage comprises the following steps: when 5% -20% of young bodies are found to be going to go in the late stage of big ear, adjusting the density of the air chambers in the water body to 1.0-2.0 square meters per square meter, and adjusting the inflation strength of the air chambers to the moving speed of air bubbles on the water surface to 5-10 cm/s; the corrugated frames are arranged at the bottom of the water body according to 2-8 per square meter, and the plate surface is upwards and transversely arranged; feeding baits and sea mud, rubbing and crushing 300-500-mesh bolting silk of the baits, then feeding, dissolving the sea mud in seawater, rubbing and crushing the sea mud by 300-500-mesh bolting silk, then feeding, and alternately feeding the baits and the sea mud;
C. the large-scale cultivation method of the young seedlings comprises the following steps: when the motion capability of young sea cucumbers with five tentacles on a corrugated plate is remarkably enhanced, young sea cucumbers are cultured in a flowing water body, the daily water change rate is 30-200% per day, bait and sea mud are fed, the bait is rubbed and crushed by a sieve with 300-500 meshes and then fed, the sea mud is dissolved in seawater and then fed after being rubbed and crushed by a sieve with 300-500 meshes, the bait and the sea mud are fed in a cross mode, the bottom of the sea cucumber is siphoned until the sea cucumber grows to be more than 1cm, and the tropical sea cucumber seedlings are obtained.
Preferably, the fertilized eggs of the tropical sea cucumbers in the step A are obtained by collecting wild tropical sea cucumber parents, transporting the wild tropical sea cucumber parents to a breeding base, inducing spawning by a negative and dry method, completing fertilization, and collecting the fertilized eggs by a 250-mesh net under the condition of not leaving seawater.
Preferably, the bait in the step A, B, C is formed by mixing chlorella powder, yeast powder, spirulina powder and sargassum thunbergii powder, wherein the chlorella powder accounts for 50-80% of the mass ratio, the rest of the species are equally divided, and the feeding amount of the bait is 0.1-1 g per square meter of water; and D, dissolving the bait in the seawater, rubbing and crushing the bait with a 300-500-mesh bolting silk screen, and feeding the bait for 2-4 times every day.
Preferably, in the step B and the step C, the bait and the sea mud are fed, the feeding amount of the sea mud is 0.5-20 times of that of the bait, the bait and the sea mud are fed in a cross mode, and the feeding frequency is 4-6 times.
Preferably, the siphon bottom in step C is siphoned once every 4-8 days.
Preferably, the seawater is obtained by filtering sand-filtered seawater with a 500-mesh filter bag, disinfecting with strong chlorine, fully aerating for 2 days, and removing residual chlorine with sodium thiosulfate. The concentration settings of the strong chlorine and the sodium thiosulfate are carried out according to a conventional method.
Preferably, ripple frame contain 5 ~ 20 buckled plates, the size and the specification of buckled plate and ripple frame are gone on according to conventional size.
Preferably, the siphon bottom needs to be wrapped by a 100-mesh bolting silk and put into a water bucket together when siphoning, a small amount of sucked attached seedlings can be attached to the 100-mesh bolting silk, the attached seedlings can be prevented from being discharged by mistake, sewage sucked out by the siphon flows into the water bucket firstly, the small amount of sucked attached seedlings is ensured not to be separated from seawater all the time, and the small amount of sucked attached seedlings can be put back into the water body for continuous cultivation after the bolting silk is slightly brushed.
The technical scheme of the invention comprises the following steps of setting reasonable cultivation density, utilizing an air pump to automatically and fully stir floating water, adopting various methods to improve the metamorphosis and attachment efficiency of larvae, reasonably feeding live algae-free baits to stabilize the water, and discloses a cultivation method of tropical sea cucumbers, wherein the cultivation method has the following beneficial effects: the air pump is used for automatically and fully stirring the floating water body, so that the manpower consumption is greatly reduced, the survival rate of floating larvae is improved in a simpler, more convenient and efficient mode, 1) the inflation density and the strength are reduced, 2) the water body attachment area is increased, 3) the water flow impact inside the water body is weakened, 4) the technical characteristics of increasing the feeding of sea mud to enable the floating larvae to be more easily settled and the like are comprehensively adopted, the metamorphosis attachment cultivation process of tropical sea cucumbers is optimized, the death rate of the tropical sea cucumbers in the attachment metamorphosis stage is remarkably reduced, in addition, on the premise of ensuring higher cultivation efficiency, the application technical scheme feeds no live algae bait, the operation is simplified, the energy consumption is reduced, the insect disease risk brought by the live algae is effectively prevented, the water body does not need to be replaced in the early stage, the controllability is remarkably improved, the cultivation efficiency of the tropical, The sustainable development and utilization of tropical sea cucumber resources provide a solid technical foundation.
The specific implementation mode is as follows:
the following examples are further illustrative of the present invention and are not intended to be limiting thereof.
In the embodiment, the seawater is obtained by filtering sand-filtered seawater by a filter bag of 500 meshes, disinfecting by strong chlorine, fully aerating for 2 days, and removing residual chlorine by sodium thiosulfate.
Example 1:
the efficient breeding method of the tropical holothuria leucospilota seedlings comprises the following steps:
A. collecting parent ginseng, hastening parturition and fertilizing: collecting 103 wild tropical holothurian parents, transporting the wild tropical holothurian parents to a breeding base by a dry method, stimulating the wild tropical holothurian parents by a negative and dry method for 2 hours, putting all the holothurians into a bucket (spawning bucket) containing 100L of seawater (the depth of the seawater is about 1m) according to 20 holothurians per bucket, paying close attention, and grabbing male holothurians producing sperms into another bucket (spermproducing bucket) containing 100L of seawater in time to allow the male holothurians to produce sperms. After the female ginseng spawns, 1L of seawater containing sperms is taken from the sperm production bucket and added into the spawning bucket, and the seawater is fully stirred to complete fertilization.
B. Adjusting the density of fertilized eggs in the cultivation water body: under the condition of not separating from seawater, collecting fertilized eggs by using a 250-mesh net, putting the fertilized eggs into clean seawater, adjusting the density of the fertilized eggs in the culture water body to 0.1/mL, namely, 10 fertilized eggs are contained in 100mL water body, and respectively applying the fertilized eggs to 2 water bodies (arranged in a cement pond) with the area of 21 square meters and the water depth of 1m to obtain about 420 ten thousand of normal fertilized eggs of the holothuria leucospilota.
C. The efficient cultivation method in the planktonic life stage comprises the following steps: in the sea water body of the indoor cement pond, the reasonable density of the air chambers and the aeration intensity of the adjusting air chambers are set, so that the cultivation water body is automatically and fully stirred, and the manual regular stirring of the water body is not needed. Specifically, the density of air chambers is set to be 0.5 square meter/air chamber in seawater, and the inflation strength of the air chambers is adjusted to the moving speed of bubbles on the water surface to be 20 cm/s; the feeding method at this stage comprises the following steps: dissolving the mixture of the chlorella powder, the yeast powder, the spirulina powder and the sargassum thunbergii powder in a small amount of clean seawater, filtering the mixture by using 400-mesh bolting silk, feeding the mixture for 3 times every day, wherein the feeding amount is 0.5g of bait per time for each square meter of cultivation water, and mixing the chlorella powder, the yeast powder, the spirulina powder and the sargassum thunbergii powder according to the mass proportion, wherein the chlorella powder accounts for 60 percent, and the rest of the species are equally divided into the rest proportion. Realizing the high-efficiency cultivation in the floating stage; when the larval later stage larval stage of shrinkage begins to appear in the planktonic larval, counting the number of the larval stage, estimating the whole number according to a density method, randomly sampling for 5 times, and finding that the average densities of 2 21 tons of water bodies are respectively 0.079 and 0.082/mL, the number of the larval later stage larval is 338.1 ten thousand in total, and the survival rate (the number of the later stage of the large ear/the number of fertilized eggs multiplied by 100%) of the planktonic stage is about 80.5%; and when 15% of the young bodies going on goblet are found in the young bodies in the later period of the big ear, adjusting the cultivation method and entering the next stage of cultivation process. The water does not need to be changed at this stage, and the method is simple, convenient and efficient.
D. The efficient cultivation method in the attachment metamorphosis stage comprises the following steps: when 15% of goblet-row larvae exist in the larvae in the late big ear period, in order to ensure that the larvae with initial metamorphosis and weak adhesive force are not influenced by water stirring and can be efficiently attached to finish the metamorphosis process, the density and the inflation strength of air chambers in the water body are required to be reduced, the density of the air chambers is set to be 1.5 square meters/per water body, and the inflation strength of the air chambers is adjusted to the movement speed of bubbles on the water surface to be 7 cm/s; meanwhile, in order to increase the attachment area, block the impact of internal water flow in the water body and protect young seedlings with weak adhesion, 5 corrugated frames (the corrugated frames comprise 20 corrugated plates, the specification of the corrugated plates is 31cm multiplied by 39cm) per square meter are arranged at the bottom of the water body in an upward and transverse manner; in order to promote the attachment of the planktonic larvae, the feeding of sea mud is added in addition to the feeding of baits. The bait feeding method comprises the following steps: dissolving a mixture of chlorella powder, yeast powder, spirulina powder and sargassum thunbergii powder in a small amount of clean seawater, filtering the mixture by 400-mesh bolting silk, and then feeding the mixture, wherein the feeding amount is 0.5g of bait per time per square meter of cultivation water, mixing the chlorella powder, the yeast powder, the spirulina powder and the sargassum thunbergii powder according to the mass proportion, the chlorella powder accounts for 60 percent, the rest is equally divided into the same types, the feeding method of the sea mud is that the feeding amount is 10 times of the bait, dissolving the sea mud in a small amount of clean seawater, and then feeding the mixture after filtering the sea mud by 400-mesh bolting silk. The sea mud feeding and the bait feeding are carried out cross feeding, the total feeding times are 5 times every day, and the high-efficiency cultivation in the stage is realized after the fact that five tentacles of young seedlings are complete and the motion capability is obviously enhanced on a corrugated plate is observed by a microscope, and the cultivation process in the next stage is started. The water does not need to be changed at this stage, and the method is simple, convenient and efficient. According to statistics, 2.8 pentahapto larvae are attached to each 10 square centimeter area on average, the area of a corrugated plate is (31 multiplied by 39) square centimeters/piece multiplied by 20 pieces/frame multiplied by 21 square meters/pool multiplied by 5 frames/square meters multiplied by 2 pools equals 507.78 ten thousand square centimeters, and the total is as follows: 142.2 ten thousand of the five tentacles larvae, the success rate of attachment (number of five tentacles/number of larvae in late stage of big ear x 100%) was about 42.1%.
E. The large-scale cultivation method of the young seedlings comprises the following steps: and D, when the motion capability of the young five-tentacle seedlings is obviously enhanced, beginning to culture the young sea cucumbers in a flowing water body, wherein the daily water change rate is 100 percent every day, feeding baits and sea mud in the feeding step D, and siphoning the bait and the sea mud once every 5 days until the sea cucumbers grow to be more than 1 cm. When siphoning bottom, 100-mesh bolting silk is needed to wrap the water outlet of the siphon and put into a bucket together, a small amount of sucked attached seedlings can be attached to the 100-mesh bolting silk, the attached seedlings can be prevented from being discharged by mistake, siphoning sewage flows into the bucket first, the small amount of sucked attached seedlings is ensured not to be separated from seawater all the time, and the small amount of sucked attached seedlings can be put back into the water body for continuous cultivation after the bolting silk is slightly brushed.
Comparative example 1:
the comparative example is basically the same as example 1, except that in step B, fertilized eggs are placed in clean seawater, the density of the fertilized eggs in the culture water body is adjusted to 0.2 fertilized eggs/mL, that is, 20 fertilized eggs are contained in 100mL water body, and the fertilized eggs are respectively placed in 1 water body with the area of 10 square meters and the water depth of 1m, so that about 200 ten thousand normal fertilized eggs of the holothuria leucospilota are obtained. Referring to example 1, the number of the larval stage was counted, the total number was estimated by the density method, the number was randomly sampled 5 times, the average density in the water was 0.008 per mL, the number of the larval later stage was 8.1 ten thousand in total, and the survival rate in the planktonic stage (the number of the larval later stage/the number of fertilized eggs × 100%) was about 4.1%. The comparative results are shown in Table 1.
Comparative example 2:
the comparative example is basically the same as the example 1, and the density of the fertilized eggs in the culture water body is adjusted to 0.1/mL, and the fertilized eggs are applied to 1 water body with the area of 21 square meters and the water depth of 1m to obtain about 210 ten thousand normal fertilized eggs of the holothuria leucospilota. The difference lies in that in the step C, referring to ZL201010604454.1 a sea cucumber seedling raising method and 201210083064.3 a novel stichopus japonicus seedling breeding method, the planktonic larvae are stirred by adopting an artificial turning method instead of an aeration method, turning is carried out for 1 time per hour, and other steps are carried out according to the example 1, so that the late stage of the big ear is obtained in total: 85.4 thousands, the survival rate (the number of late big ears/the number of fertilized eggs multiplied by 100%) in the floating stage is about 40.7%. The comparative results are shown in Table 1.
Comparative example 3:
the comparative example is basically the same as the example 1, and about 210 ten thousand normal fertilized eggs of the holothuria leucospilota are obtained by adjusting the density of the fertilized eggs in the culture water body to 0.1/mL and applying the fertilized eggs in 1 water body with the area of 21 square meters and the water depth of 1 m. The difference lies in that in the step D, the density of the air chambers is not reduced, the inflation intensity is not reduced, the density of the air chambers in the water body is continuously kept to be 0.5 square meter/piece, and the inflation intensity of the air chambers is adjusted to the movement speed of the bubbles on the water surface to be 20 cm/s. 168.5 million larvae in the later period of the big ear and 15.6 million larvae in the later period of the big ear are obtained in total, and the attachment success rate (the number of the five tentacles/the number of the larvae in the later period of the big ear multiplied by 100%) is about 9.3%. The comparative results are shown in Table 1.
Comparative example 4:
the comparative example is basically the same as the example 1, and about 150 thousands of normal fertilized eggs of the holothuria leucospilota are obtained by adjusting the density of the fertilized eggs in the culture water body to 0.1/mL and applying the fertilized eggs in 1 water body with the area of 15 square meters and the water depth of 1 m. Except that the bait is rubbed and crushed by a 200-mesh bolting silk and fed, and other steps are the same as the example 1. In total, 111.8 thousands of larvae in the later period of big ears are obtained, the survival rate (the number of the later period of the big ears/the number of fertilized eggs multiplied by 100%) in the floating stage is about 74.5%, 16.4 thousands of larvae of five tentacles are obtained, and the attachment success rate (the number of the five tentacles/the number of the larvae in the later period of the big ears multiplied by 100%) is about 14.7%. The comparative results are shown in Table 1.
Table 1 comparison table of cultivation effect of different examples
The technical scheme of the embodiment 1 is different from that of the comparative example 1 in analysis: comparative example 1 the density of the planktonic larvae is too high, and in the implementation process, the planktonic larvae are found to have a significantly slower development speed and have a sharply reduced number after the planktonic larvae develop to the middle ear larvae, which indicates that the reasonable density of the planktonic larvae is important for the success of the technical scheme of the present application.
The technical scheme of the embodiment 1 and the comparative example 2 is analyzed in a distinguishing way: in the comparative example 2, the planktonic larvae are cultivated by adopting an artificial turning method, and the fact that the labor consumption is large and the survival rate of the planktonic larvae is remarkably lower than that of the planktonic larvae in the example 1 shows that the technical scheme of the application makes remarkable progress.
The technical scheme of the embodiment 1 and the comparative example 3 is analyzed in a distinguishing way: comparative example 3 in step D, without decreasing the density of the air cells and without decreasing the aeration intensity, it was found that the planktonic larvae could not attach at this stage and were always in a planktonic state, and after 5 days of duration, dead embryos began to appear in the planktonic larvae, and the final attachment rate was very low, indicating that the sea cucumber in the metamorphosis stage no longer required vigorous agitation by water, and at this time, decreasing the density and aeration intensity of the air cells could significantly contribute to metamorphosis of the larvae.
The technical scheme of the embodiment 1 and the comparative example 4 is analyzed in a distinguishing way: comparative example 4 the bait fed was grated through a 200 mesh bolting silk, and the comparison found: the size of the bait has certain influence on the survival rate in the floating period, the phenomenon of inconsistent development speed occurs in the process of culturing the size of the bait, the size of the bait has obvious influence on the attachment rate, and most of the larvae are failed to be fed in the initial stage due to the fact that the larvae need to be fed in the initial stage again after being attached, and the particle size is too large.
Claims (8)
1. An efficient cultivation method of tropical sea cucumber seedlings is characterized by comprising the following steps:
A. the efficient cultivation method in the planktonic life stage comprises the following steps: putting the fertilized eggs of the tropical sea cucumbers into seawater to enable the density of the fertilized eggs in a water body of the tropical sea cucumbers to be 0.05-0.15/mL, setting the density of air chambers in the water body to be 0.5-1.0 square meter/mL, adjusting the inflation strength of the air chambers to the moving speed of air bubbles on the water surface to be 15-30 cm/s, feeding baits, rubbing and crushing the baits through a 300-500-mesh bolting silk and then feeding the baits;
B. the efficient cultivation method in the attachment metamorphosis stage comprises the following steps: when 5% -20% of young bodies are found to be going to go in the late stage of big ear, adjusting the density of the air chambers in the water body to 1.0-2.0 square meters per square meter, and adjusting the inflation strength of the air chambers to the moving speed of air bubbles on the water surface to 5-10 cm/s; the corrugated frames are arranged at the bottom of the water body according to 2-8 per square meter, and the plate surface is upwards and transversely arranged; feeding baits and sea mud, rubbing and crushing 300-500-mesh bolting silk of the baits, then feeding, dissolving the sea mud in seawater, rubbing and crushing the sea mud by 300-500-mesh bolting silk, then feeding, and alternately feeding the baits and the sea mud;
C. the large-scale cultivation method of the young seedlings comprises the following steps: when the motion capability of young sea cucumbers with five tentacles on a corrugated plate is remarkably enhanced, young sea cucumbers are cultured in a flowing water body, the daily water change rate is 30-200% per day, bait and sea mud are fed, the bait is rubbed and crushed by a sieve with 300-500 meshes and then fed, the sea mud is dissolved in seawater and then fed after being rubbed and crushed by a sieve with 300-500 meshes, the bait and the sea mud are fed in a cross mode, the bottom of the sea cucumber is siphoned until the sea cucumber grows to be more than 1cm, and the tropical sea cucumber seedlings are obtained.
2. The efficient cultivation method of tropical sea cucumber seedlings according to claim 1, wherein the fertilized eggs of tropical sea cucumber in the step A are obtained by collecting wild tropical sea cucumber parents, transporting the wild tropical sea cucumber parents to a breeding base, inducing spawning by a negative dry method, completing fertilization, and collecting the fertilized eggs by a 250-mesh net without leaving seawater.
3. The efficient cultivation method of tropical sea cucumber seedlings according to claim 1, wherein the bait in the step A, B, C is formed by mixing chlorella powder, yeast powder, spirulina powder and sargassum thunbergii powder, wherein the chlorella powder accounts for 50-80% of the mass ratio, the rest of the species are equally divided, and the feeding amount of the bait is 0.1-1 g per square meter of water; and D, dissolving the bait in the seawater, rubbing and crushing the bait with a 300-500-mesh bolting silk screen, and feeding the bait for 2-4 times every day.
4. The efficient cultivation method of tropical sea cucumber seedlings according to claim 1 or 3, wherein the bait and the sea mud are fed in the steps B and C, the feeding amount of the sea mud is 0.5-20 times of that of the bait, the bait and the sea mud are fed in a cross mode, and the feeding frequency is 4-6 times.
5. The method for efficiently cultivating young tropical sea cucumbers according to claim 1, wherein the siphon bottom in the step C is siphoned every 4-8 days.
6. The efficient cultivation method of tropical sea cucumber seedlings according to claim 1, wherein the seawater is sand-filtered seawater, which is filtered by a 500-mesh filter bag, sterilized by strong chlorine, fully aerated for 2 days, and then subjected to removal of residual chlorine by sodium thiosulfate.
7. The efficient cultivation method of tropical sea cucumber seedlings according to claim 1, wherein the corrugated frame comprises 5-20 corrugated plates.
8. The method for efficiently cultivating tropical sea cucumber seedlings according to claim 1, wherein the siphon bottom is formed by wrapping a water outlet of a siphon tube with a 100-mesh bolting silk and putting the siphon tube and the 100-mesh bolting silk into a water bucket during siphoning, a small amount of sucked attached seedlings are attached to the 100-mesh bolting silk, siphoning sewage flows into the water bucket first to ensure that the small amount of sucked attached seedlings are not separated from seawater all the time, and washing the bolting silk and then putting the sucked attached seedlings back into the water body for continuous cultivation.
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| CN113796474A (en) * | 2021-09-30 | 2021-12-17 | 中国科学院南海海洋研究所 | Compound feed for culturing tropical sea cucumber larvae in planktonic period and preparation method thereof |
| CN114223589A (en) * | 2022-01-25 | 2022-03-25 | 中国科学院南海海洋研究所 | Bottom sowing proliferation method for tropical sea cucumber seedlings in open sea area |
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| CN114223589B (en) * | 2022-01-25 | 2023-03-17 | 中国科学院南海海洋研究所 | Bottom sowing proliferation method for tropical sea cucumber fries in open sea area |
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