CN114342844A - Breeding method of holothuria fuscogongensis - Google Patents
Breeding method of holothuria fuscogongensis Download PDFInfo
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- CN114342844A CN114342844A CN202210096937.8A CN202210096937A CN114342844A CN 114342844 A CN114342844 A CN 114342844A CN 202210096937 A CN202210096937 A CN 202210096937A CN 114342844 A CN114342844 A CN 114342844A
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Abstract
The invention discloses a breeding method of southern rough sea cucumbers, which comprises the steps of building a sea cucumber raising pond, culturing parent sea cucumbers, spawning and fertilization of the parent sea cucumbers, culturing larvae, culturing young sea cucumbers and the like, wherein the parent sea cucumbers are mature rough sea cucumbers locally caught in the south, a plurality of preferential breeding new species of rough sea cucumbers are obtained by the steps of catching the wild sea cucumbers in the sea, culturing the parent sea cucumbers intensively, hastening spawning fertilized eggs, culturing the larvae, culturing the young sea cucumbers, screening and breeding excellent characters and the like, so that the method can adapt to culture in different scales, ensure environmental modes of adapting to pond culture, mudflat culture and the like, and simultaneously, the rough sea cucumbers are controlled to regulate and control diversity, so that the adaptability of the rough sea cucumbers is stronger, the breeding is easier, the excellent rough sea cucumber strains with stronger growth performance are selected, beneficial references are provided for the rough sea cucumber breeding, culturing and scientific research, and the southern sea cucumber chain is better developed, creating good social and economic benefits.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a breeding method of holothuria fuscogongensis.
Background
Brown sea cucumber, with the scientific name: holothuria scabra is an animal of the sea cucumber genus of the family Holothuria, commonly known as Yushen, straight ladybell root and white ginseng, is a high-quality edible sea cucumber, has the characteristics of high protein, low fat and low sugar, has very high nutritional value, economic value and health care function, and is a real ecological healthy food; the holothuria scabra is also an important tropical holothurian culture variety and a top-grade aquatic product which is imported from exports internationally; distributed in areas such as Guangxi, Guangdong, Hainan, etc. from Nata hong Kong to Red sea, eastern to Carolina, Fijiri, North to Japan, southward to Australia and China continental, generally live at the bank reef edge and at the sand bottom where the tidal water flow rate is fast and the sea weed is plenty; as an important link in holothurian culture, the holothurian seedling raising technology is widely developed in coastal areas of China.
The quality control of breeding water is poor when the existing holothuria fuscogutva is bred and bred, the growth of seedlings is easily influenced by the temperature, salinity and purity of the water, the seedlings cannot grow in a good breeding environment, the foraging amount of the seedlings is low, the characters are poor, excellent holothuria fuscogutva strains cannot be obtained, and an effective method for breeding the holothuria fuscogutva parent sea cucumber is not explored at present.
Disclosure of Invention
In view of the above, the invention provides a breeding method of holothuria fuscogongensis, aiming at solving the technical problems.
In order to achieve the purpose, the invention adopts the following technical scheme:
a breeding method of holothuria fuscogongensis comprises the following steps:
s1, building a ginseng cultivation pool: building a special ginseng culturing pool, wherein a water outlet is formed in the center of the bottom of the ginseng culturing pool, and a water inlet pipe and a plurality of inflation nozzles are arranged in the ginseng culturing pool; at least one layer of attachment is arranged in the ginseng culturing pool;
s2, parent ginseng cultivation: collecting mature Holothuria scabra in natural sea area as parent sea cucumber, stocking in a sea cucumber pond for cultivation, wherein stocking density is 15-20 heads/square meter; feeding once every evening, wherein the feeding bait for the parent ginseng comprises sargassum thunbergii powder and parent ginseng compound feed; continuously flowing water and aerating, wherein the water temperature is 25-30 ℃, the salinity is 25-32, the dissolved oxygen is more than 5mg/L, and residual baits at the bottom of the pond are periodically removed;
s3, spawning and fertilization of the parent ginseng: inducing rough sea cucumber to lay eggs in the spawning season, selecting healthy and strong parent sea cucumbers, washing the surfaces of the sea cucumbers with filtered seawater, then placing the sea cucumbers in a sea cucumber raising pool, drying the sea cucumbers in the open air for 30-50min, then stimulating the sea cucumbers with filtered seawater for 30-50min, then injecting seawater, and waiting for the sperm and eggs to be discharged; stirring once every 40 minutes to prevent the sperm and egg accumulation, fishing out the parent ginseng after the sperm and egg discharge is finished, changing water to remove redundant sperm, and oozing the fertilized egg in a ginseng cultivation pond;
s4, larva cultivation: after the fertilized eggs are hatched and developed into larvae, carrying out siphon breeding to breed the middle and upper layer larvae, and controlling the stocking density of the larvae to be 0.2-0.4 per milliliter; feeding chlorella, Chaetoceros and Platymonas 3-4 times a day; when 25% -30% of the larvae are transformed into goblet-shaped larvae, putting the seedling collector until the attachment of the goblet-shaped larvae is finished;
s5, young sea cucumber cultivation: after the goblet-shaped larvae are attached and transformed into young sea cucumbers, benthic diatoms, aquatic yeast, bacillus and young sea cucumbers are used as baits and are respectively fed once every morning and evening; culturing under the conditions of micro-flowing water and continuous aeration, wherein the dissolved oxygen is more than 5 mg/L; periodically pouring the pond to remove residual bait and excrement at the bottom of the pond; when the young sea cucumbers grow to reach the body length of 0.8-1.2cm, the young sea cucumbers are peeled off from the bottom of the pool to be continuously cultivated; when the young sea cucumbers grow to be more than 2cm, the young sea cucumbers are used for pond or mudflat culture.
Preferably, the ginseng culture pond is of a cuboid structure with an opening at the top, and the depth of the ginseng culture pond is 1.0-1.2 meters.
Preferably, the water inlet pipe is divided into a cold water inlet pipe and a hot water inlet pipe for adjusting the water temperature.
Preferably, the frequency of periodically removing the bottom dead baits in the step of S2 is 1-time of siphon-clearing the bottom dead baits every 3 to 5 days.
Preferably, the ratio of the sargassum thunbergii powder to the parent ginseng compound feed in the parent ginseng feeding bait in the step S2 is 1: 2.
Preferably, the seed collector in the step S4 adopts a corrugated plate, and is placed into a bait pool to inoculate benthic diatoms before being thrown.
Preferably, the ratio of the chlorella to the chaetoceros to the tetraselmis in the larva cultivation bait obtained in the step S4 is 1:1.5: 2.
According to the technical scheme, compared with the prior art, the invention discloses the breeding method of the holothuria fuscogongensis in south, the stocking density of the holothuria fuscogongensis is controlled, the sargassum thunbergii powder and the holothuria fuscogongensis compound feed are used as baits for feeding, the nutrition is enhanced, and the breeding intensity of the holothuria fuscogongensis is enhanced; the natural baits are used for feeding the sea cucumber larvae, so that excrement and the like generated by the sea cucumber larvae can be oxidized and reduced into harmless substances, and the transparency of the water body can be effectively adjusted through the natural baits such as algae and the like; the stocking density of the larvae is reasonably adjusted, and the diseases of the sea cucumber larvae are reduced; through systematic and reasonable breeding steps of the holothuria scabra, excellent characters of holothuria seedlings are screened and bred, a holothuria variety with larger size, strong adaptability and strong vitality is obtained, excellent holothuria scabra strains are bred, beneficial references are provided for breeding, seedling raising, breeding and scientific research of holothuria scabra varieties, the southern holothuria industrial chain is better developed, and good social and economic benefits are created.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a breeding method of southern rough sea cucumbers, which comprises the steps of building a sea cucumber raising pond, culturing parent sea cucumbers, spawning and fertilization of the parent sea cucumbers, culturing larvae, culturing young sea cucumbers and the like, wherein the parent sea cucumbers are mature rough sea cucumbers locally caught in the south, a plurality of preferential breeding new species of rough sea cucumbers are obtained by the steps of catching the wild sea cucumbers in the sea, culturing the parent sea cucumbers intensively, hastening spawning fertilized eggs, culturing the larvae, culturing the young sea cucumbers, screening and breeding excellent characters and the like, so that the method can adapt to culture in different scales, ensure environmental modes of adapting to pond culture, mudflat culture and the like, and simultaneously, the rough sea cucumbers are controlled to regulate and control diversity, so that the adaptability of the rough sea cucumbers is stronger, the breeding is easier, the excellent rough sea cucumber strains with stronger growth performance are selected, beneficial references are provided for the rough sea cucumber breeding, culturing and scientific research, and the southern sea cucumber chain is better developed, creating good social and economic benefits.
The breeding method of holothuria fuscogongensis specifically comprises the following steps:
s1, building a ginseng cultivation pool: building a special ginseng culturing pool, wherein a water outlet is formed in the center of the bottom of the ginseng culturing pool, and a water inlet pipe and a plurality of inflation nozzles are arranged in the ginseng culturing pool; at least one layer of attachment is arranged in the ginseng culturing pool;
s2, parent ginseng cultivation: collecting mature Holothuria scabra in natural sea area as parent sea cucumber, stocking in a sea cucumber pond for cultivation, wherein stocking density is 15-20 heads/square meter; feeding once every evening, wherein the feeding bait for the parent ginseng comprises sargassum thunbergii powder and parent ginseng compound feed; continuously flowing water and aerating, wherein the water temperature is 25-30 ℃, the salinity is 25-32, the dissolved oxygen is more than 5mg/L, and residual baits at the bottom of the pond are periodically removed;
s3, spawning and fertilization of the parent ginseng: inducing rough sea cucumber to lay eggs in the spawning season, selecting healthy and strong parent sea cucumbers, washing the surfaces of the sea cucumbers with filtered seawater, then placing the sea cucumbers in a sea cucumber raising pool, drying the sea cucumbers in the open air for 30-50min, then stimulating the sea cucumbers with filtered seawater for 30-50min, then injecting seawater, and waiting for the sperm and eggs to be discharged; stirring once every 40 minutes to prevent the sperm and egg accumulation, fishing out the parent ginseng after the sperm and egg discharge is finished, changing water to remove redundant sperm, and oozing the fertilized egg in a ginseng cultivation pond;
s4, larva cultivation: after the fertilized eggs are hatched and developed into larvae, carrying out siphon breeding to breed the middle and upper layer larvae, and controlling the stocking density of the larvae to be 0.2-0.4 per milliliter; feeding chlorella, Chaetoceros and Platymonas 3-4 times a day; when 25% -30% of the larvae are transformed into goblet-shaped larvae, putting the seedling collector until the attachment of the goblet-shaped larvae is finished;
s5, young sea cucumber cultivation: after the goblet-shaped larvae are attached and transformed into young sea cucumbers, benthic diatoms, aquatic yeast, bacillus and young sea cucumbers are used as baits and are respectively fed once every morning and evening; culturing under the conditions of micro-flowing water and continuous aeration, wherein the dissolved oxygen is more than 5 mg/L; periodically pouring the pond to remove residual bait and excrement at the bottom of the pond; when the young sea cucumbers grow to reach the body length of 0.8-1.2cm, the young sea cucumbers are peeled off from the bottom of the pool to be continuously cultivated; when the young sea cucumbers grow to be more than 2cm, the young sea cucumbers are used for pond or mudflat culture.
In order to further optimize the technical scheme, the ginseng culture pond is of a cuboid structure with an opening at the top, and the depth is 1.0-1.2 meters.
In order to further optimize the technical scheme, the water inlet pipe is divided into a cold water inlet pipe and a hot water inlet pipe which are used for adjusting the water temperature.
In order to further optimize the above technical means, the frequency of periodically removing the bottom bait in the step of S2 is 1-time of siphon-clearing the bottom bait every 3 to 5 days.
In order to further optimize the technical scheme, the ratio of sargassum thunbergii powder to the parent ginseng compound feed in the parent ginseng feeding bait in the step S2 is 1: 2.
In order to further optimize the technical scheme, the seed collector in the step S4 adopts a corrugated plate, and the seed collector is placed into a bait pool to inoculate benthic diatoms before being put into the bait pool.
In order to further optimize the technical scheme, the ratio of the chlorella to the chaetoceros to the tetraselmis in the larva cultivation bait in the step S4 is 1:1.5: 2.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A breeding method of holothuria fuscogongensis is characterized by comprising the following steps:
s1, building a ginseng cultivation pool: building a special ginseng culturing pool, wherein a water outlet is formed in the center of the bottom of the ginseng culturing pool, and a water inlet pipe and a plurality of inflation nozzles are arranged in the ginseng culturing pool; at least one layer of attachment is arranged in the ginseng culturing pool;
s2, parent ginseng cultivation: collecting mature Holothuria scabra in natural sea area as parent sea cucumber, stocking in a sea cucumber pond for cultivation, wherein stocking density is 15-20 heads/square meter; feeding once every evening, wherein the feeding bait for the parent ginseng comprises sargassum thunbergii powder and parent ginseng compound feed; continuously flowing water and aerating, wherein the water temperature is 25-30 ℃, the salinity is 25-32, the dissolved oxygen is more than 5mg/L, and residual baits at the bottom of the pond are periodically removed;
s3, spawning and fertilization of the parent ginseng: inducing rough sea cucumber to lay eggs in the spawning season, selecting healthy and strong parent sea cucumbers, washing the surfaces of the sea cucumbers with filtered seawater, then placing the sea cucumbers in a sea cucumber raising pool, drying the sea cucumbers in the open air for 30-50min, then stimulating the sea cucumbers with filtered seawater for 30-50min, then injecting seawater, and waiting for the sperm and eggs to be discharged; stirring once every 40 minutes to prevent the sperm and egg accumulation, fishing out the parent ginseng after the sperm and egg discharge is finished, changing water to remove redundant sperm, and oozing the fertilized egg in a ginseng cultivation pond;
s4, larva cultivation: after the fertilized eggs are hatched and developed into larvae, carrying out siphon breeding to breed the middle and upper layer larvae, and controlling the stocking density of the larvae to be 0.2-0.4 per milliliter; feeding chlorella, Chaetoceros and Platymonas 3-4 times a day; when 25% -30% of the larvae are transformed into goblet-shaped larvae, putting the seedling collector until the attachment of the goblet-shaped larvae is finished;
s5, young sea cucumber cultivation: after the goblet-shaped larvae are attached and transformed into young sea cucumbers, benthic diatoms, aquatic yeast, bacillus and young sea cucumbers are used as baits and are respectively fed once every morning and evening; culturing under the conditions of micro-flowing water and continuous aeration, wherein the dissolved oxygen is more than 5 mg/L; periodically pouring the pond to remove residual bait and excrement at the bottom of the pond; when the young sea cucumbers grow to reach the body length of 0.8-1.2cm, the young sea cucumbers are peeled off from the bottom of the pool to be continuously cultivated; when the young sea cucumbers grow to be more than 2cm, the young sea cucumbers are used for pond or mudflat culture.
2. The breeding method of holothuria fuscogilva var nanensis according to claim 1, wherein the holothuria nanensis pool is a cuboid structure with an open top and a depth of 1.0-1.2 m.
3. The selective breeding method of holothuria fuscogilva according to claim 1, wherein the water inlet pipe is divided into a cold water inlet pipe and a hot water inlet pipe for adjusting water temperature.
4. The method for breeding holothuria fuscogensis according to claim 1, wherein the frequency of removing the bottom bait periodically in step S2 is 1 time per 3-5 days of siphon.
5. The breeding method of holothuria fuscogongensis according to claim 1, wherein the ratio of sargassum thunbergii powder to the parent sea cucumber compound feed in the parent sea cucumber feeding bait in the step S2 is 1: 2.
6. The breeding method of holothuria fuscogongensis according to claim 1, wherein the seed collector in the step S4 is corrugated plate, and the seed collector is placed into a bait pool before being put into a bait pool to inoculate benthic diatoms.
7. The breeding method of holothuria fuscogongensis according to claim 1, wherein the ratio of chlorella, chaetoceros and platymysternon in the larval culture bait in the step S4 is 1:1.5: 2.
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