CN104540382A - 用于产生条件性敲除等位基因的方法和组合物 - Google Patents

用于产生条件性敲除等位基因的方法和组合物 Download PDF

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CN104540382A
CN104540382A CN201380042657.8A CN201380042657A CN104540382A CN 104540382 A CN104540382 A CN 104540382A CN 201380042657 A CN201380042657 A CN 201380042657A CN 104540382 A CN104540382 A CN 104540382A
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sequence
donor
recombinase
cell
homology
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S·瓦明
K·R·安德森
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F Hoffmann La Roche AG
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine

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  • Biodiversity & Conservation Biology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380042657.8A 2012-06-12 2013-06-12 用于产生条件性敲除等位基因的方法和组合物 Pending CN104540382A (zh)

Applications Claiming Priority (3)

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US201261658670P 2012-06-12 2012-06-12
US61/658,670 2012-06-12
PCT/US2013/045382 WO2013188522A2 (en) 2012-06-12 2013-06-12 Methods and compositions for generating conditional knock-out alleles

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US (1) US20150128300A1 (enExample)
EP (1) EP2858486A4 (enExample)
JP (1) JP6279562B2 (enExample)
KR (1) KR20150023670A (enExample)
CN (1) CN104540382A (enExample)
BR (1) BR112014031080A2 (enExample)
CA (1) CA2876076A1 (enExample)
HK (1) HK1209276A1 (enExample)
MX (1) MX2014015204A (enExample)
RU (1) RU2014153918A (enExample)
WO (1) WO2013188522A2 (enExample)

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CN107779462A (zh) * 2016-08-29 2018-03-09 中国科学院上海生命科学研究院 双同源重组谱系示踪技术
CN108998483A (zh) * 2018-08-17 2018-12-14 武汉核圣生物技术有限公司 利用单亚基RNA聚合酶进行体外转录合成sgRNA的方法
CN109072245A (zh) * 2016-02-26 2018-12-21 朗泽科技新西兰有限公司 用于c1固定菌的crispr/cas系统
CN109688810A (zh) * 2016-06-27 2019-04-26 贝勒医学院 具有缺陷性p450氧化还原酶的人肝嵌合非人动物及其使用方法
CN113557408A (zh) * 2019-05-29 2021-10-26 孟山都技术公司 用于使用基因组编辑产生显性矮株型等位基因的方法和组合物
US12473564B2 (en) 2019-05-29 2025-11-18 Monsanto Technology Llc Methods and compositions for generating dominant alleles using genome editing

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HK1209276A1 (en) 2016-04-01
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JP2015519082A (ja) 2015-07-09
JP6279562B2 (ja) 2018-02-14
BR112014031080A2 (pt) 2018-05-08
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