CN104395464A - 改善的脂肪酸衍生物的生产 - Google Patents
改善的脂肪酸衍生物的生产 Download PDFInfo
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- CN104395464A CN104395464A CN201380026304.9A CN201380026304A CN104395464A CN 104395464 A CN104395464 A CN 104395464A CN 201380026304 A CN201380026304 A CN 201380026304A CN 104395464 A CN104395464 A CN 104395464A
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Abstract
Description
缩写 | 完整描述 |
St_fabD | 鼠伤寒沙门氏杆菌fabD基因 |
nSt_fabH | 具有天然RBS的鼠伤寒沙门氏杆菌fabH基因 |
sSt_fabH | 具有合成RBS的鼠伤寒沙门氏杆菌fabH基因 |
Cac_fabF | 丙酮丁醇梭菌(ATCC824)fabF基因 |
St_fabG | 鼠伤寒沙门氏杆菌fabG基因 |
St_fabA | 鼠伤寒沙门氏杆菌fabA基因 |
St_fabZ | 鼠伤寒沙门氏杆菌fabZ基因 |
BS_fabl | 枯草芽孢杆菌fabl基因 |
BS_FabL | 枯草芽孢杆菌fabL基因 |
Vc_FabV | 霍乱弧菌fabV基因 |
Ec_FabI | 大肠埃希氏杆菌fabI基因 |
ifab | BS_fabI | BS_fabL | Vc_fabV | Ec_fabI | nSt_fabH | sSt_fabH | St_fabD | St_fabG | St_fabA | St_fabZ | Cac_fabF |
ifab130 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 |
ifab131 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
ifab132 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 |
ifab133 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
ifab134 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 |
ifab135 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
ifab136 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 |
ifab137 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
ifab138 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 |
ifab139 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
ifab140 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 |
ifab141 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
ifab142 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 |
ifab143 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
ifab144 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 |
ifab145 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
质粒 | 基因 |
pTB.071 | pDS57-accCBDA |
pTB.072 | pDS57-accCBDA-birA |
pTB.073 | pDS57-accDACB |
pTB.074 | pDS57-accDACB-birA |
菌株 | 其他fab操纵子的基因型 | 质粒 |
stEP117 | DAM1ΔinsH::PLACUV5(snyRBS)EcfabH(snyRBS)BsfabI::kan | pDS57 |
stEP118 | DAM1ΔinsH::PLACUV5(snyRBS)EcfabH(snyRBS)BsfabL::kan | pDS57 |
stEP127 | DAM1ΔinsH::PLACUV5(EcRBS)EcfabH(EcRBS)BsfabI::kan | pDS57 |
stEP128 | DAM1ΔinsH::PLACUV5(EcRBS)EcfabH(EcRBS)BsfabL::kan | pDS57 |
stEP129 | DAM1ΔinsH::PLACUV5(EcRBS)ADP1fabH(EcRBS)ADP1fabI::kan | pDS57 |
stEP130 | DAM1ΔinsH::PLACUV5(snyRBS)ADP1fabH(synRBS)ADP1fabI::kan | pDS57 |
stEP131 | DAM1ΔinsH::PLACUV5(snyRBS)VT8fabH1(synRBS)VT8fabI::kan | pDS57 |
stEP132 | DAM1ΔinsH::PLACUV5(snyRBS)VT8fabH2(synRBS)VT8fabI::kan | pDS57 |
stEP133 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH1(synRBS)VT8fabI::kan | pDS57 |
stEP134 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH2(synRBS)VT8fabI::kan | pDS57 |
stEP151 | DAM1ΔinsH::PLACUV5(snyRBS)RofabI(synRBS)RofabH::kan | pDS57 |
stEP153 | DAM1ΔinsH::PLACUV5(EcRBS)ADP1fabH(EcRBS)ADP1fabI::kan | pDS57-accCBDA |
stEP154 | DAM1ΔinsH::PLACUV5(EcRBS)ADP1fabH(EcRBS)ADP1fabI::kan | pDS57-accDACB |
stEP155 | DAM1ΔinsH::PLACUV5(EcRBS)ADP1fabH(EcRBS)ADP1fabI::kan | pDS57-accCBDA-birA |
stEP156 | DAM1ΔinsH::PLACUV5(EcRBS)ADP1fabH(EcRBS)ADP1fabI::kan | pDS57-accDACB-birA |
stEP157 | DAM1ΔinsH::PLACUV5(snyRBS)EcfabH(synRBS)BsfabI::kan | pDS57-accCBDA |
stEP158 | DAM1ΔinsH::PLACUV5(snyRBS)EcfabH(synRBS)BsfabI::kan | pDS57-accCBDA-bvirA |
stEP159 | DAM1ΔinsH::PLACUV5(EcRBS)EcfabH(synRBS)BsfabI::kan | pDS57-accCBDA |
stEP160 | DAM1ΔinsH::PLACUV5(EcRBS)EcfabH(synRBS)BsfabI::kan | pDS57-accCBDA-birA |
stEP161 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH1(synRBS)VT8fabI::kan | pDS57-accCBDA |
stEP162 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH1(synRBS)VT8fabI::kan | pDS57-accCBDA-birA |
stEP163 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH2(synRBS)VT8fabI::kan | pDS57-accCBDA |
stEP164 | DAM1ΔinsH::PLACUV5(EcRBS)VT8fabH2(synRBS)VT8fabI::kan | pDS57-accCBDA-birA |
菌株 | FAS效价 | FAS产率 | FALC百分率 | FALC产率 |
V940 | 68g/L | 18.7% | 95.0% | 17.8% |
D851 | 70g/L | 19.4% | 96.1% | 18.6% |
V940 | 64g/L | 18.4% | 91.9% | 16.9% |
D851 | 67g/L | 19.0% | 94.0% | 17.8% |
成分 | 浓度 |
(NH4)2SO4 | 0.5g/L |
KH2PO4 | 3.0g/L |
柠檬酸铁 | 0.034g/L |
TM2微量矿物溶液 | 10mL/L |
酪蛋白氨基酸 | 5g/L |
灭菌后加入 | |
MgSO4-7H2O | 2.2g/L |
微量维生素溶液 | 1.25mL/L |
葡萄糖 | 5g/L |
接种物 | 50mL/L |
Claims (51)
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PCT/US2013/035037 WO2013152051A2 (en) | 2012-04-02 | 2013-04-02 | Improved production of fatty acid derivatives |
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US (4) | US20150064782A1 (zh) |
EP (2) | EP3674400A1 (zh) |
JP (3) | JP6381518B2 (zh) |
KR (2) | KR102166271B1 (zh) |
CN (2) | CN114456996A (zh) |
AU (1) | AU2013243601B2 (zh) |
BR (3) | BR122019026142B1 (zh) |
CA (2) | CA3162104A1 (zh) |
CO (1) | CO7170149A2 (zh) |
MX (2) | MX366939B (zh) |
MY (1) | MY181688A (zh) |
WO (1) | WO2013152051A2 (zh) |
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CN111936623A (zh) * | 2018-03-30 | 2020-11-13 | 英威达纺织(英国)有限公司 | 用于生物合成制造庚二酸的材料与方法和合成多肽的利用 |
CN111936623B (zh) * | 2018-03-30 | 2023-08-25 | 英威达纺织(英国)有限公司 | 用于生物合成制造庚二酸的材料与方法和合成多肽的利用 |
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