CN104195088A - Chromogenic culture medium for enterobacter sakazakii - Google Patents
Chromogenic culture medium for enterobacter sakazakii Download PDFInfo
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- CN104195088A CN104195088A CN201410458104.7A CN201410458104A CN104195088A CN 104195088 A CN104195088 A CN 104195088A CN 201410458104 A CN201410458104 A CN 201410458104A CN 104195088 A CN104195088 A CN 104195088A
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Abstract
The invention relates to the field of microorganisms and particularly relates to a chromogenic culture medium for enterobacter sakazakii. The culture medium comprises the following components: a triple sugar iron (TSI) agar culture medium, 1.0g/L ammonium ferric citrate, 2.0g/L sodium thiosulfate, 2.0g/L sodium deoxycholate, 0.5g/L monopotassium phosphate, 0.2g/L 5-bromo-4-chloro-3-indol-alpha-D-glucopyranoside, 15.0g/L agar and 0.1g/L color developing agent, wherein the pH value of the culture medium is 7.1-7.5, and every liter of the TSI agar culture medium contains 15.0g of tryptone, 5.0g of soy peptone, 3.0g of beef extract powder, 3.0g of yeast powder, 5.0g of sodium chloride, 1.0g of glucose, 10.0g of sucrose, 0.2g of ferrous sulfate, 0.3g of sodium thiosulfate, 12.0g of agar, 0.04g of bromcresol purple and the balance of distilled water. The culture medium disclosed by the invention is simple in formula, convenient in preparation operation, low in cost and beneficial to popularization and application; bacteria are rapid in growth and low in aberration rate. The culture medium is convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of Enterobacter sakazaii colour development culture medium.
Background technology
The ecological complexity in the variation between microorganism and variability and species habitat in the world, has brought discussion, has understood popularity, complicacy and the permanence of microorganism.Species for microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, has formed the sensitive regulation system of a set of complete unification, rely on this regulation system, strictly control various Metabolic activities.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, different because of culture medium prescription, affect its Form index situation.
Substratum (Medium) is for the nutriment of microorganism, plant and animal tissue growth and the artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single kind microorganism culturing and evaluation.Substratum is because the raw material of preparation is different, and service requirements is different, and storage and custody aspect is also slightly different.General substratum is being heated, after the moisture absorption, is easily contaminated by bacterial or decomposes rottenly, and therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of strict sterilizing, and the storage of long period, must be placed in the refrigerator of 3～6 ℃.Because liquid nutrient medium is difficult for long-term keeping, all change system into now powder.
Substratum is natural medium, combination substratum according to chemical classification and partly combines substratum; According to physical classification, be liquid nutrient medium, film solid media and dehydrated medium; According to microorganism classification, be selective medium, differential medium.
Summary of the invention
The present invention overcomes deficiency of the prior art, and a kind of Enterobacter sakazaii colour development culture medium is provided.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, the row filter that is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factors such as the pH value of substratum, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding are compared, worked out substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of Enterobacter sakazaii colour development culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles-α-D-of the bromo-4-of 0.2g/L5-pyranoglucose glucoside, 15.0g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, and all the other are distilled water.
Take this product 8.62g and add 200ml distilled water, heated and boiled is dissolved completely, 121 ℃ of autoclaving 15min, and while being chilled to 50 ℃ of left and right, the aseptic plate of impouring.
The flat board preparing can be preserved 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 ℃, notes keeping in Dark Place.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of Enterobacter sakazaii colour development culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles-α-D-of the bromo-4-of 0.2g/L5-pyranoglucose glucoside, 15.0g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, and all the other are distilled water.
Take this product 8.62g and add 200ml distilled water, heated and boiled is dissolved completely, 121 ℃ of autoclaving 15min, and while being chilled to 50 ℃ of left and right, the aseptic plate of impouring.
The flat board preparing can be preserved 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 ℃, notes keeping in Dark Place.
Embodiment in above-mentioned embodiment is illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore do not departing from any variation and the modification of making under general plotting of the present invention, within all should belonging to the protection domain that the present invention limits.
Claims (1)
1. an Enterobacter sakazaii colour development culture medium, the component that it is characterized in that described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles-α-D-of the bromo-4-of 0.2g/L5-pyranoglucose glucoside, 15.0g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, and all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410458104.7A CN104195088A (en) | 2014-09-09 | 2014-09-09 | Chromogenic culture medium for enterobacter sakazakii |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410458104.7A CN104195088A (en) | 2014-09-09 | 2014-09-09 | Chromogenic culture medium for enterobacter sakazakii |
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| CN201410458104.7A Pending CN104195088A (en) | 2014-09-09 | 2014-09-09 | Chromogenic culture medium for enterobacter sakazakii |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861623A (en) * | 2016-04-25 | 2016-08-17 | 无锡市赛微生物技术有限公司 | Chromogenic culture medium for detecting Enterobacter sakazakii |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423862A (en) * | 2008-12-19 | 2009-05-06 | 广东环凯微生物科技有限公司 | Enterobacter sakazaii colour development culture medium, detection kit and detection method |
| CN104651247A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Enterobacter sakazakii chromogenic medium |
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2014
- 2014-09-09 CN CN201410458104.7A patent/CN104195088A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423862A (en) * | 2008-12-19 | 2009-05-06 | 广东环凯微生物科技有限公司 | Enterobacter sakazaii colour development culture medium, detection kit and detection method |
| CN104651247A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Enterobacter sakazakii chromogenic medium |
Non-Patent Citations (1)
| Title |
|---|
| 何莹等: "1株与致病性大肠埃希氏菌O114∶K90(B)交叉凝集的阪崎肠杆菌", 《首都公共卫生》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861623A (en) * | 2016-04-25 | 2016-08-17 | 无锡市赛微生物技术有限公司 | Chromogenic culture medium for detecting Enterobacter sakazakii |
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