CN104651456A - Lauryl sulfate tryptone MUG medium - Google Patents
Lauryl sulfate tryptone MUG medium Download PDFInfo
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- CN104651456A CN104651456A CN201310599834.4A CN201310599834A CN104651456A CN 104651456 A CN104651456 A CN 104651456A CN 201310599834 A CN201310599834 A CN 201310599834A CN 104651456 A CN104651456 A CN 104651456A
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- China
- Prior art keywords
- lauryl sulfate
- agar
- mug
- tryptone
- medium
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Abstract
The invention relates to the field of microbes and especially relates to a lauryl sulfate tryptone MUG medium. The lauryl sulfate tryptone MUG medium comprises a trisaccharide iron (TSI) agar medium, 1.0g/L of disodium hydrogen phosphate, 0.1g/L of sodium lauryl sulfate, 10.0g/L of dipotassium hydrogen phosphate, 0.5g/L of potassium dihydrogen phosphate, 2.5g/L of phenethyl alcohol, 0.3g/L of MUG, 15.0g/L of agar and 0.1g/L of a color developing agent. The lauryl sulfate tryptone MUG medium has a pH value of 6.6-7.0. Each liter of the TSI agar medium comprises 15.0g of tryptone, 3.0g of beef extract powder, 3.0g of yeast powder, 5.0g of common salt, 5.0g of proteose peptone, 10.0g of lactose, 1.0g of glucose, 10.0g of cane sugar, 0.2g of ferrous sulphate, 0.3g of sodium hyposulfite, 12.0g of agar, 0.04g of bromcresol purple and the balance distilled water. The lauryl sulfate tryptone MUG medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of lauryl sulfate Tryptones MUG substratum.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of lauryl sulfate Tryptones MUG substratum.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of lauryl sulfate Tryptones MUG substratum, it is characterized in that the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L Sodium phosphate dibasic, 0.1g/L Sodium Lauryl Sulphate BP/USP, 10.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 2.5g/L phenylethyl alcohol, 0.3g/LMUG, 15.0 g/L agar, 0.1g/L developer, pH value is 6.6-7.0; Described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Take this product 64.5g, heating for dissolving is in 1000ml distilled water, and heated and boiled 1 minute, is sub-packed in 16mm × 150mm test tube, 121 DEG C of autoclavings, 15 minutes long 4-5cm of bevel, the long 2-3cm in bottom.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of lauryl sulfate Tryptones MUG substratum, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L Sodium phosphate dibasic, 0.1g/L Sodium Lauryl Sulphate BP/USP, 10.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 2.5g/L phenylethyl alcohol, 0.3g/LMUG, 15.0 g/L agar, 0.1g/L developer, pH value is 6.6-7.0; Described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Take this product 64.5g, heating for dissolving is in 1000ml distilled water, and heated and boiled 1 minute, is sub-packed in 16mm × 150mm test tube, 121 DEG C of autoclavings, 15 minutes long 4-5cm of bevel, the long 2-3cm in bottom.
| Quality-control strains | Growing state | Further feature |
| Intestinal bacteria | +++ | Aerogenesis, unstressed configuration |
| Colon bacillus | +++ | Aerogenesis, has fluorescence |
| Salmonellas | +++ | Not aerogenesis, unstressed configuration |
| Streptococcus aureus | - | / |
Claims (1)
1. a lauryl sulfate Tryptones MUG substratum, it is characterized in that the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L Sodium phosphate dibasic, 0.1g/L Sodium Lauryl Sulphate BP/USP, 10.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 2.5g/L phenylethyl alcohol, 0.3g/LMUG, 15.0 g/L agar, 0.1g/L developer, pH value is 6.6-7.0; Described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599834.4A CN104651456A (en) | 2013-11-25 | 2013-11-25 | Lauryl sulfate tryptone MUG medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599834.4A CN104651456A (en) | 2013-11-25 | 2013-11-25 | Lauryl sulfate tryptone MUG medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651456A true CN104651456A (en) | 2015-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310599834.4A Pending CN104651456A (en) | 2013-11-25 | 2013-11-25 | Lauryl sulfate tryptone MUG medium |
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| CN (1) | CN104651456A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293667A (en) * | 2014-09-29 | 2015-01-21 | 青岛康合伟业商贸有限公司 | Lauryl sulfate tryptose MUG medium |
-
2013
- 2013-11-25 CN CN201310599834.4A patent/CN104651456A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293667A (en) * | 2014-09-29 | 2015-01-21 | 青岛康合伟业商贸有限公司 | Lauryl sulfate tryptose MUG medium |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |