CN104651463A - Fuchsin sulfite agar and use thereof - Google Patents
Fuchsin sulfite agar and use thereof Download PDFInfo
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- CN104651463A CN104651463A CN201310599328.5A CN201310599328A CN104651463A CN 104651463 A CN104651463 A CN 104651463A CN 201310599328 A CN201310599328 A CN 201310599328A CN 104651463 A CN104651463 A CN 104651463A
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- Prior art keywords
- agar
- fuchsin
- sulfite agar
- sodium sulfite
- meat soup
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Abstract
The invention relates to the field of microbes and especially relates to fuchsin sulfite agar. The fuchsin sulfite agar comprises EC broth, 5.0g/L of beef powder, 5.0g/L of yeast powder, 1.0g/L of basic fuchsin, 6.0g/L of sodium hydrosulphite and 15.0g/L of agar. The fuchsin sulfite agar has a pH value of 7.2-7.4. Each liter of the EC broth comprises 20.0g of tryptone, 10.0g of lactose, 2.0g of potassium dihydrogen phosphate, 5.0g of common salt, 4.0g of dipotassium hydrogen phosphate and the balance distilled water. The fuchsin sulfite agar has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of fuchsin basic sodium sulfite agar and application thereof.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of fuchsin basic sodium sulfite agar and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of fuchsin basic sodium sulfite agar, the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, and pH value is 7.2-7.4;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
The invention also discloses the application of described fuchsin basic sodium sulfite agar, for tap water, the selective separation of total coli group and confirmation in source water.
Take this product 52.5g, add 20ml dehydrated alcohol and 980ml distilled water, heated and stirred is dissolved, packing, and 116 DEG C of autoclavings 20 minutes are for subsequent use.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of fuchsin basic sodium sulfite agar, the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, and pH value is 7.2-7.4;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
Take this product 52.5g, add 20ml dehydrated alcohol and 980ml distilled water, heated and stirred is dissolved, packing, and 116 DEG C of autoclavings 20 minutes are for subsequent use.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | - | - |
| Colon bacillus | +++ | Red-purple bacterium colony, there is metalluster |
| Salmonellas | +++ | Colony colourless is transparent |
Claims (3)
1. a fuchsin basic sodium sulfite agar, it is characterized in that the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, pH value is 7.2-7.4.
2. fuchsin basic sodium sulfite agar according to claim 1, it is characterized in that described EC meat soup is for containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup, all the other are distilled water.
3. an application for fuchsin basic sodium sulfite agar according to claim 1, is characterized in that for tap water, the selective separation of total coli group and confirmation in source water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599328.5A CN104651463A (en) | 2013-11-25 | 2013-11-25 | Fuchsin sulfite agar and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599328.5A CN104651463A (en) | 2013-11-25 | 2013-11-25 | Fuchsin sulfite agar and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651463A true CN104651463A (en) | 2015-05-27 |
Family
ID=53243103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310599328.5A Pending CN104651463A (en) | 2013-11-25 | 2013-11-25 | Fuchsin sulfite agar and use thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN104651463A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263806A (en) * | 2014-09-29 | 2015-01-07 | 青岛康合伟业商贸有限公司 | Fuchsin basic sodium sulfite agar and application thereof |
-
2013
- 2013-11-25 CN CN201310599328.5A patent/CN104651463A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263806A (en) * | 2014-09-29 | 2015-01-07 | 青岛康合伟业商贸有限公司 | Fuchsin basic sodium sulfite agar and application thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |