CN104651452A - Improved sorbitol-MacConkey agar medium - Google Patents
Improved sorbitol-MacConkey agar medium Download PDFInfo
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- CN104651452A CN104651452A CN201310599749.8A CN201310599749A CN104651452A CN 104651452 A CN104651452 A CN 104651452A CN 201310599749 A CN201310599749 A CN 201310599749A CN 104651452 A CN104651452 A CN 104651452A
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- sorbitol
- agar medium
- macconkey agar
- substratum
- macconkey
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the field of microbes and relates to an improved sorbitol-MacConkey agar medium. The improved sorbitol-MacConkey agar medium comprises a mTSB broth agar medium, 3.0g/L of polypeptone, 10.0g/L of sorbitol, 0.5g/L of cow bile salt, 0.2g/L of cysteine hydrochloride, 15.0g/L of agar, 0.02g/L of neutral red and 0.002g/L of crystal violet. The improved sorbitol-MacConkey agar medium has a pH value of 7.3-7.5. Each liter of the mTSB broth agar medium contains 17.0g of tryptone, 3.0g of soy peptone, 5.0g of common salt, 1.5g of bile salt No.3, 4.0g of dipotassium hydrogen phosphate, 2.5g of glucose, 0.3g of novobiocin and the balance distilled water. The improved sorbitol-MacConkey agar medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly one improvement sorbitol-MacConkey agar substratum.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of improvement sorbitol-MacConkey agar substratum.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of improvement sorbitol-MacConkey agar substratum, the component of described substratum comprises mTSB broth agar culture medium, 3.0g/L multivalent protein peptone, 10.0g/L sorbyl alcohol, 0.5g/L bovine bile, 0.2g/L cysteine hydrochloride, 15.0 g/L agar, 0.02g/L toluylene red, 0.002g/L Viola crystallina pH value is 7.3-7.5;
On the basis of above scheme, described improvement sorbitol-MacConkey agar substratum, described mTSB broth agar culture medium be in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5 g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Take this product 51.5g, heating for dissolving is in 1000ml distilled water, packing triangular flask, every bottle of 200ml, when 121 DEG C of autoclavings are chilled to 45 DEG C ± about 1 DEG C for 15 minutes, adds an improvement sorbitol-MacConkey agar additive (containing Cefixime Micronized 0.01mg, potassium tellurite 0.5mg), mixing, impouring sterilized petri dishes, for subsequent use.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of improvement sorbitol-MacConkey agar substratum, the component of described substratum comprises mTSB broth agar culture medium, 3.0g/L multivalent protein peptone, 10.0g/L sorbyl alcohol, 0.5g/L bovine bile, 0.2g/L cysteine hydrochloride, 15.0 g/L agar, 0.02g/L toluylene red, 0.002g/L Viola crystallina pH value is 7.3-7.5;
Described improvement sorbitol-MacConkey agar substratum, described mTSB broth agar culture medium be in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5 g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Take this product 51.5g, heating for dissolving is in 1000ml distilled water, packing triangular flask, every bottle of 200ml, when 121 DEG C of autoclavings are chilled to 45 DEG C ± about 1 DEG C for 15 minutes, adds an improvement sorbitol-MacConkey agar additive (containing Cefixime Micronized 0.01mg, potassium tellurite 0.5mg), mixing, impouring sterilized petri dishes, for subsequent use.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
| Bacterium name | Upgrowth situation | Colony characteristics |
| Colon bacillus O157:H7 | Well | Colony colourless, center beige |
| Colon bacillus | Suppress | － |
| Streptococcus aureus | Suppress | － |
| Proteus vulgaris | Suppress | － |
Claims (2)
1. an improvement sorbitol-MacConkey agar substratum, it is characterized in that the component of described substratum comprises mTSB broth agar culture medium, 3.0g/L multivalent protein peptone, 10.0g/L sorbyl alcohol, 0.5g/L bovine bile, 0.2g/L cysteine hydrochloride, 15.0 g/L agar, 0.02g/L toluylene red, 0.002g/L Viola crystallina pH value is 7.3-7.5.
2. improvement sorbitol-MacConkey agar substratum according to claim 1, it is characterized in that described mTSB broth agar culture medium in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5 g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599749.8A CN104651452A (en) | 2013-11-25 | 2013-11-25 | Improved sorbitol-MacConkey agar medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599749.8A CN104651452A (en) | 2013-11-25 | 2013-11-25 | Improved sorbitol-MacConkey agar medium |
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| CN104651452A true CN104651452A (en) | 2015-05-27 |
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| CN201310599749.8A Pending CN104651452A (en) | 2013-11-25 | 2013-11-25 | Improved sorbitol-MacConkey agar medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277972A (en) * | 2014-09-29 | 2015-01-14 | 青岛康和食品有限公司 | Improved sorbitol macconkey agar culture medium |
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2013
- 2013-11-25 CN CN201310599749.8A patent/CN104651452A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104277972A (en) * | 2014-09-29 | 2015-01-14 | 青岛康和食品有限公司 | Improved sorbitol macconkey agar culture medium |
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Application publication date: 20150527 |