CN104651445A - Urethra positioning chromogenic medium and use thereof - Google Patents
Urethra positioning chromogenic medium and use thereof Download PDFInfo
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- CN104651445A CN104651445A CN201310599329.XA CN201310599329A CN104651445A CN 104651445 A CN104651445 A CN 104651445A CN 201310599329 A CN201310599329 A CN 201310599329A CN 104651445 A CN104651445 A CN 104651445A
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- urethra
- positioning
- chromogenic medium
- broth base
- medium
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of microbes and especially relates to a urethra positioning chromogenic medium. The urethra positioning chromogenic medium comprises bromcersol purple broth base, 2.0g/L of dipotassium hydrogen phosphate, 5.2g/L of sodium hyposulfite, 0.5g/L of ferric ammonium citrate, 1.0g/L of Tween 80, 15.0g/L of agar and 0.1g/L of a color developing agent. The urethra positioning chromogenic medium has a pH value of 7.2-7.4. Each liter of the bromcersol purple broth base comprises 10.0g of tryptone, 3.0g of beef extract powder, 5.0g of common salt, 0.5g of magnesium sulfate, 0.04g of bromcresol purple and the balance distilled water. The urethra positioning chromogenic medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of urethra CHROMagar orientation medium and application thereof.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of urethra CHROMagar orientation medium and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of urethra CHROMagar orientation medium, the component of described substratum comprises bromcresol purple broth base, 2.0g/L dipotassium hydrogen phosphate, 5.2g/L Sulfothiorine, 0.5g/L Ferric Ammonium Citrate, 1.0g/L tween 80,15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4.
On the basis of above scheme, described urethra CHROMagar orientation medium, described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, magnesium sulfate 0.5g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
The invention discloses the application of described urethra CHROMagar orientation medium, for separating of with discriminating common urethra pathogenic bacterium.
Take this product 22.6g, add in 200ml distilled water or purified water, heating for dissolving does not stop to stir, and boils and does not exceed 1 minute.Substratum water-bath after heating is cooled to 45-50 DEG C, shakes up lightly, impouring sterilized petri dishes (the 15ml/ ware of 9cm, the 10ml/ ware of 7cm), after agar solidifies completely, upside down 0.5-1 hour in the incubator of 36 DEG C ± 1 DEG C, for subsequent use.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of urethra CHROMagar orientation medium, the component of described substratum comprises bromcresol purple broth base, 2.0g/L dipotassium hydrogen phosphate, 5.2g/L Sulfothiorine, 0.5g/L Ferric Ammonium Citrate, 1.0g/L tween 80,15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4.
Described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, magnesium sulfate 0.5g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
Take this product 22.6g, add in 200ml distilled water or purified water, heating for dissolving does not stop to stir, and boils and does not exceed 1 minute.Substratum water-bath after heating is cooled to 45-50 DEG C, shakes up lightly, impouring sterilized petri dishes (the 15ml/ ware of 9cm, the 10ml/ ware of 7cm), after agar solidifies completely, upside down 0.5-1 hour in the incubator of 36 DEG C ± 1 DEG C, for subsequent use.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
Claims (3)
1. a urethra CHROMagar orientation medium, it is characterized in that the component of described substratum comprises bromcresol purple broth base, 2.0g/L dipotassium hydrogen phosphate, 5.2g/L Sulfothiorine, 0.5g/L Ferric Ammonium Citrate, 1.0g/L tween 80,15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4.
2. urethra CHROMagar orientation medium according to claim 1, it is characterized in that described bromcresol purple broth base is for containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, magnesium sulfate 0.5g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base, all the other are distilled water.
3. an application for urethra CHROMagar orientation medium according to claim 1, it is characterized in that for for separating of with discriminating common urethra pathogenic bacterium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599329.XA CN104651445A (en) | 2013-11-25 | 2013-11-25 | Urethra positioning chromogenic medium and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599329.XA CN104651445A (en) | 2013-11-25 | 2013-11-25 | Urethra positioning chromogenic medium and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651445A true CN104651445A (en) | 2015-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201310599329.XA Pending CN104651445A (en) | 2013-11-25 | 2013-11-25 | Urethra positioning chromogenic medium and use thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263647A (en) * | 2014-09-30 | 2015-01-07 | 青岛康合伟业商贸有限公司 | Urethral positioning chromogenic medium and application thereof |
-
2013
- 2013-11-25 CN CN201310599329.XA patent/CN104651445A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104263647A (en) * | 2014-09-30 | 2015-01-07 | 青岛康合伟业商贸有限公司 | Urethral positioning chromogenic medium and application thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |