CN104278075A - Escherichia coli chromogenic culture medium - Google Patents
Escherichia coli chromogenic culture medium Download PDFInfo
- Publication number
- CN104278075A CN104278075A CN201410502529.3A CN201410502529A CN104278075A CN 104278075 A CN104278075 A CN 104278075A CN 201410502529 A CN201410502529 A CN 201410502529A CN 104278075 A CN104278075 A CN 104278075A
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- culture medium
- escherichia coli
- broth agar
- mtsb
- chromogenic
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of microorganisms and particularly relates to an escherichia coli chromogenic culture medium. The culture medium comprises the following components: a mTSB broth agar culture medium, 1.0g/L ammonium ferric citrate, 2.0g/L sodium thiosulfate, 0.5g/L potassium dihydrogen phosphate, 15.0g/L agar and 0.2g/L chromogenic reagent, and the pH value of the culture medium is 7.1-7.5; and according to the escherichia coli chromogenic culture medium, each liter of the mTSB broth agar culture medium contains 17.0g of tryptone, 3.0g of soy peptone, 5.0g of sodium chloride, 1.5g of bale salt No. 3, 4.0g of dipotassium phosphate, 2.5g of glucose, 0.3g of novobiocin and the balance of distilled water. The culture medium provided by the invention is simple in formula, convenient in preparation operation, low in cost, beneficial to popularization and application and convenient to observe and analyze; and bacteria are rapid in growth and low in mutation probability.
Description
Technical field
The present invention is microorganism field, particularly a kind of slope e.coli chromogenic medium.
Background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.
Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.
Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of e.coli chromogenic medium.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of e.coli chromogenic medium, the component of described substratum comprises mTSB broth agar culture medium, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 0.5g/L potassium primary phosphate 0.2g/L, 15.0g/L agar, 0.2g/L developer, pH value is 7.1-7.5; Described mTSB broth agar culture medium be in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Take this product 15g and add 200ml distilled water, heated and boiled is dissolved completely, 121 DEG C of autoclaving 15min, when being chilled to about 50 DEG C, and impouring sterilized petri dishes.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of e.coli chromogenic medium, the component of described substratum comprises mTSB broth agar culture medium, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 0.5g/L potassium primary phosphate 0.2g/L, 15.0g/L agar, 0.2g/L developer, pH value is 7.1-7.5; Described mTSB broth agar culture medium be in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Take this product 15g and add 200ml distilled water, heated and boiled is dissolved completely, 121 DEG C of autoclaving 15min, when being chilled to about 50 DEG C, and impouring sterilized petri dishes.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
| Quality-control strains | Growing state | Colony colour |
| Single increasing Lee Salmonella | -/+ | Colourless |
| Streptococcus aureus | -/+ | Colourless |
| Intestinal bacteria | +++ | Blue |
| Salmonellas | +++ | Colourless |
| Coliform | +++ | Blue |
Embodiment in above-mentioned embodiment is illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore do not departing from any change and amendment made under general plotting of the present invention, all should belong within protection domain that the present invention limits.
Claims (1)
1. an e.coli chromogenic medium, it is characterized in that the component of described substratum comprises mTSB broth agar culture medium, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 0.5g/L potassium primary phosphate 0.2g/L, 15.0g/L agar, 0.2g/L developer, pH value is 7.1-7.5; Described mTSB broth agar culture medium be in often liter of mTSB broth agar culture medium containing Tryptones 17.0g, soy peptone 3.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g, glucose 2.5g, Vulkamycin. PA-93 0.3g, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410502529.3A CN104278075A (en) | 2014-09-26 | 2014-09-26 | Escherichia coli chromogenic culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410502529.3A CN104278075A (en) | 2014-09-26 | 2014-09-26 | Escherichia coli chromogenic culture medium |
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| Publication Number | Publication Date |
|---|---|
| CN104278075A true CN104278075A (en) | 2015-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410502529.3A Pending CN104278075A (en) | 2014-09-26 | 2014-09-26 | Escherichia coli chromogenic culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434426A (en) * | 2016-08-30 | 2017-02-22 | 内蒙古金源康生物工程有限公司 | High-efficiency expression culture medium for escherichia coli |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294190A (en) * | 2007-12-05 | 2008-10-29 | 姚毓才 | Composite quick colour-developing examination and check agent for coliform group bacteria, researching and developing flow scheme thereof |
| CN102424832A (en) * | 2011-11-23 | 2012-04-25 | 广东环凯微生物科技有限公司 | Chromogenic medium used for detecting esherichia coli O157:H7 |
| CN104651451A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Escherichia coli chromogenic medium |
-
2014
- 2014-09-26 CN CN201410502529.3A patent/CN104278075A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294190A (en) * | 2007-12-05 | 2008-10-29 | 姚毓才 | Composite quick colour-developing examination and check agent for coliform group bacteria, researching and developing flow scheme thereof |
| CN102424832A (en) * | 2011-11-23 | 2012-04-25 | 广东环凯微生物科技有限公司 | Chromogenic medium used for detecting esherichia coli O157:H7 |
| CN104651451A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Escherichia coli chromogenic medium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434426A (en) * | 2016-08-30 | 2017-02-22 | 内蒙古金源康生物工程有限公司 | High-efficiency expression culture medium for escherichia coli |
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Application publication date: 20150114 |