CN104195217A - Staphylococcus aureus chromogenic culture media - Google Patents
Staphylococcus aureus chromogenic culture media Download PDFInfo
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- CN104195217A CN104195217A CN201410462279.5A CN201410462279A CN104195217A CN 104195217 A CN104195217 A CN 104195217A CN 201410462279 A CN201410462279 A CN 201410462279A CN 104195217 A CN104195217 A CN 104195217A
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- staphylococcus aureus
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Abstract
The invention belongs to the field of microorganisms and in particular relates to a staphylococcus aureus chromogenic culture media. The culture media comprises the following components: a trisaccharide iron (TSI) agar culture media, 12 g/L casein peptone, 6 g/L mannitol, 2.0 g/L dipotassium hydrogen phosphate, 0.5 g/L potassium dihydrogen phosphate, 8 g/L glycine, 12 g/L sodium pyruvate and a 0.16 g/L color developing agent, wherein the pH value is 7.2-7.4. According to the staphylococcus aureus chromogenic culture media, 1 L of the TSI agar culture media contains 15.0 g of tryptone, 10.0 g of soybean peptone, 5.0 g of yeast powder, 4.0 g of sodium chloride, 10.0 g of sucrose, 0.2 g of ferrous sulfate, 0.3 g of sodium thiosulfate, 15.0 g of agar, 0.04 g of bromcresol purple and the balance of distilled water. The staphylococcus aureus chromogenic culture media is simple in component, convenient to prepare, low in cost and favorable for popularization and application; staphylococcus aurei are high in growth speed, low in aberration rate and convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of streptococcus aureus color developing culture medium.
Background technology
The ecological complexity in the variation between microorganism and variability and species habitat in the world, has brought discussion, has understood popularity, complicacy and the permanence of microorganism.Species for microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, has formed the sensitive regulation system of a set of complete unification, rely on this regulation system, strictly control various Metabolic activities.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because of culture medium prescription difference, affects its Form index situation.
Substratum (Medium) is for the nutriment of microorganism, plant and animal tissue growth and the artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single kind microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, service requirements difference, and storage and custody aspect is also slightly different.General substratum is being heated, after the moisture absorption, be easily contaminated by bacterial or decompose rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of strict sterilizing, and the storage of long period, must be placed in the refrigerator of 3～6 DEG C.Because liquid nutrient medium is difficult for long-term keeping, all change system into now powder.
Substratum is natural medium, combination substratum according to chemical classification and partly combines substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer and calculate at present the culture medium prescription of applicable a certain bacterial classification completely from the ultimate principle of biochemical reaction, can only use the basic theories of biological chemistry, cytobiology, microbiology etc., the empirical formula that is applicable to a certain class bacterial classification using with reference to forefathers, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipments such as shaking flask, glass pot, select comparatively applicable substratum according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider while determining according to forefathers' experience and medium component, tentatively determine possible medium component
Test and finally determine the most suitable medium component by single-factor
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These are tested often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, and a kind of streptococcus aureus color developing culture medium is provided.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, to the row filter that is combined into of all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulant, the materialization factors such as pH value to substratum, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding compare, and have worked out substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of streptococcus aureus color developing culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 12g/L casein peptone, 6g/L N.F,USP MANNITOL, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 8g/L glycine, 12g/L Sodium.alpha.-ketopropionate, developer 0.16g/L, and pH value is 7.2-7.4.
On the basis of above scheme, described streptococcus aureus color developing culture medium, described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, and all the other are distilled water.
Take this product 55.0g, heating for dissolving in 1000ml distilled water, packing,, every bottle of 200ml, 121 DEG C of autoclavings 15 minutes, while being chilled to 50 DEG C of left and right, every bottle adds 1% potassium tellurite solution 4ml of filtration sterilization, mixes, for subsequent use.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of streptococcus aureus color developing culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 12g/L casein peptone, 6g/L N.F,USP MANNITOL, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 8g/L glycine, 12g/L Sodium.alpha.-ketopropionate, developer 0.16g/L, and pH value is 7.2-7.4.
Described streptococcus aureus color developing culture medium, described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, and all the other are distilled water.
Take this product 55.0g, heating for dissolving in 1000ml distilled water, packing,, every bottle of 200ml, 121 DEG C of autoclavings 15 minutes, while being chilled to 50 DEG C of left and right, every bottle adds 1% potassium tellurite solution 4ml of filtration sterilization, mixes, for subsequent use.
Cultivate 24～28h hour at 36 ± 1 DEG C:
| Quality Control bacterial strain | Growing state | Colony colour | Opaque ring |
| Single increasing Lee Salmonella | + | Purple | - |
| Streptococcus aureus | +++ | Blue-greenish colour | + |
| Staphylococcus epidermidis | +++ | Colourless | - |
| Salmonellas | -/+ | Colourless | - |
| Intestinal bacteria | -/+ | Colourless | - |
Claims (2)
1. a streptococcus aureus color developing culture medium, the component that it is characterized in that described substratum comprises triple sugariron (TSI) nutrient agar, 12g/L casein peptone, 6g/L N.F,USP MANNITOL, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 8g/L glycine, 12g/L Sodium.alpha.-ketopropionate, developer 0.16g/L, and pH value is 7.2-7.4.
2. streptococcus aureus color developing culture medium according to claim 1, it is characterized in that described triple sugariron (TSI) nutrient agar is to contain Tryptones 15.0g, soy peptone 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g in every liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410462279.5A CN104195217A (en) | 2014-09-12 | 2014-09-12 | Staphylococcus aureus chromogenic culture media |
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| CN201410462279.5A CN104195217A (en) | 2014-09-12 | 2014-09-12 | Staphylococcus aureus chromogenic culture media |
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| CN104195217A true CN104195217A (en) | 2014-12-10 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106566780A (en) * | 2015-10-08 | 2017-04-19 | 中创云牧(内蒙古)科技咨询有限公司 | Method for enriching staphylococcus aureus in milk |
| CN109251957A (en) * | 2018-10-17 | 2019-01-22 | 长春理工大学 | Staphylococcus aureus selective coloration culture medium testing piece |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186891A (en) * | 2007-12-21 | 2008-05-28 | 广东环凯微生物科技有限公司 | Salmonella color culture medium, detection kit an detection method |
| CN101423862A (en) * | 2008-12-19 | 2009-05-06 | 广东环凯微生物科技有限公司 | Enterobacter sakazaii colour development culture medium, detection kit and detection method |
| CN101555514A (en) * | 2009-05-14 | 2009-10-14 | 浙江工商大学 | Vibrio mimicus chromogenic culture medium |
| CN104651467A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Staphylococcus aureus chromogenic medium |
-
2014
- 2014-09-12 CN CN201410462279.5A patent/CN104195217A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186891A (en) * | 2007-12-21 | 2008-05-28 | 广东环凯微生物科技有限公司 | Salmonella color culture medium, detection kit an detection method |
| CN101423862A (en) * | 2008-12-19 | 2009-05-06 | 广东环凯微生物科技有限公司 | Enterobacter sakazaii colour development culture medium, detection kit and detection method |
| CN101555514A (en) * | 2009-05-14 | 2009-10-14 | 浙江工商大学 | Vibrio mimicus chromogenic culture medium |
| CN104651467A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Staphylococcus aureus chromogenic medium |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106566780A (en) * | 2015-10-08 | 2017-04-19 | 中创云牧(内蒙古)科技咨询有限公司 | Method for enriching staphylococcus aureus in milk |
| CN109251957A (en) * | 2018-10-17 | 2019-01-22 | 长春理工大学 | Staphylococcus aureus selective coloration culture medium testing piece |
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Application publication date: 20141210 |