CN104313115A - Improved tomato juice culture medium and application thereof - Google Patents
Improved tomato juice culture medium and application thereof Download PDFInfo
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- CN104313115A CN104313115A CN201410503060.5A CN201410503060A CN104313115A CN 104313115 A CN104313115 A CN 104313115A CN 201410503060 A CN201410503060 A CN 201410503060A CN 104313115 A CN104313115 A CN 104313115A
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Abstract
The invention relates to the field of microorganisms and in particular relates to an improved tomato juice culture medium and an application thereof. The improved tomato juice culture medium comprises the following components: a triple sugar iron (TSI) agar culture medium, 2.5g/L of tomato powder, 6g/L of sodium acetate, 2.0g/L of dipotassium phosphate, 0.5g/L of monopotassium phosphate and 0.6g/L of Tween 80, and pH value of the improved tomato juice culture medium is 6.6-7.0; and one litre of triple sugar iron (TSI) agar culture medium contains the following components: 15.0g of tryptone, 10.0g of beef extract powder, 5.0g of yeast powder, 4.0g of sodium chloride, 5.0g of proteose peptone, 20.0g of lactose, 1.0g of glucose, 10.0g of cane sugar, 0.2g of ferrous sulfate, 0.3g of sodium thiosulfate, 15.0g of agar, 0.04g of bromcresol purple and balance of distilled water. The improved tomato juice culture medium is simple in composition, easy to prepare and operate, low in cost and beneficial to popularization and application; bacteria grow quickly, and mutation rate is low; and observation and analysis are facilitated.
Description
Technical field
The present invention is microorganism field, particularly one improvement tomato juice substratum and application thereof.
Background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.
Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer from the ultimate principle of biochemical reaction completely and calculate the culture medium prescription of applicable a certain bacterial classification at present, the basic theories of biological chemistry, cytobiology, microbiology etc. can only be used, with reference to the empirical formula being comparatively applicable to a certain class bacterial classification that forefathers use, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipment such as shaking flask, glass pot, select the substratum be comparatively applicable to according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider when determining according to the experience of forefathers and medium component, tentatively determine possible medium component
Medium component the most suitable is finally determined by single factor experiment
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These experiments, often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of improvement tomato juice substratum.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of improvement tomato juice substratum, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 2.5g/L tomato powder, 6g/L sodium acetate, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 0.6g/L tween 80, and pH value is 6.6-7.0;
On the basis of above scheme, described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, showing peptone 5.0g, lactose 20.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
The invention also discloses the application of described improvement tomato juice substratum, measure for milk-acid bacteria sum in food.
Take this product 62.5g, heating for dissolving in 1000ml distilled water, packing, 121 DEG C of autoclavings 15 minutes, for subsequent use.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of improvement tomato juice substratum, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 2.5g/L tomato powder, 6g/L sodium acetate, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 0.6g/L tween 80, and pH value is 6.6-7.0;
Described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, showing peptone 5.0g, lactose 20.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Take this product 62.5g, heating for dissolving in 1000ml distilled water, packing, 121 DEG C of autoclavings 15 minutes, for subsequent use.
Cultivate 72 ± 3 hours at 36 ± 1 DEG C.
Embodiment in above-mentioned embodiment is illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore do not departing from any change and amendment made under general plotting of the present invention, all should belong within protection domain that the present invention limits.
Claims (3)
1. an improvement tomato juice substratum, it is characterized in that the component of described substratum comprises triple sugariron (TSI) nutrient agar, 2.5g/L tomato powder, 6g/L sodium acetate, 2.0g/L dipotassium hydrogen phosphate, 0.5g/L potassium primary phosphate, 0.6g/L tween 80, pH value is 6.6-7.0.
2. improvement tomato juice substratum according to claim 1, it is characterized in that described triple sugariron (TSI) nutrient agar is for containing Tryptones 15.0g, beef extract powder 10.0g, yeast powder 5.0g, sodium-chlor 4.0g, showing peptone 5.0g, lactose 20.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2g, Sulfothiorine 0.3g, agar 15.0g, purpurum bromocresolis 0.04g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
3. an application for improvement tomato juice substratum according to claim 1, is characterized in that measuring for milk-acid bacteria sum in food.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410503060.5A CN104313115A (en) | 2014-09-26 | 2014-09-26 | Improved tomato juice culture medium and application thereof |
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| CN201410503060.5A CN104313115A (en) | 2014-09-26 | 2014-09-26 | Improved tomato juice culture medium and application thereof |
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| CN104313115A true CN104313115A (en) | 2015-01-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757544A (en) * | 2015-04-13 | 2015-07-08 | 光明乳业股份有限公司 | Dietary supplement with bacteriostatic action and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732470A (en) * | 2012-07-20 | 2012-10-17 | 光明乳业股份有限公司 | Purely natural and low-cost lactobacillus (Lb.) plantarum ST-III culture method and products thereof, and application of products |
| CN103215161A (en) * | 2013-03-14 | 2013-07-24 | 河南科技大学 | Method for reducing acidity of wild Kiwi berry wine using Oenococcus oeni |
| CN104651459A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Improved tomato juice medium and use thereof |
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2014
- 2014-09-26 CN CN201410503060.5A patent/CN104313115A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732470A (en) * | 2012-07-20 | 2012-10-17 | 光明乳业股份有限公司 | Purely natural and low-cost lactobacillus (Lb.) plantarum ST-III culture method and products thereof, and application of products |
| CN103215161A (en) * | 2013-03-14 | 2013-07-24 | 河南科技大学 | Method for reducing acidity of wild Kiwi berry wine using Oenococcus oeni |
| CN104651459A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Improved tomato juice medium and use thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104757544A (en) * | 2015-04-13 | 2015-07-08 | 光明乳业股份有限公司 | Dietary supplement with bacteriostatic action and preparation method and application thereof |
| CN104757544B (en) * | 2015-04-13 | 2017-11-14 | 光明乳业股份有限公司 | A kind of dietary supplements with bacteriostasis and its preparation method and application |
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