CN104195213A - Vibrio chromogenic culture medium - Google Patents
Vibrio chromogenic culture medium Download PDFInfo
- Publication number
- CN104195213A CN104195213A CN201410442803.2A CN201410442803A CN104195213A CN 104195213 A CN104195213 A CN 104195213A CN 201410442803 A CN201410442803 A CN 201410442803A CN 104195213 A CN104195213 A CN 104195213A
- Authority
- CN
- China
- Prior art keywords
- agar
- tsi
- medium
- culture medium
- sodium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the field of microorganisms, and in particular relates to a vibrio chromogenic culture medium which comprises the following components: a trisaccharide iron (TSI) agar culture-medium, 0.5g/L disodium hydrogen phosphate, 0.4g/L monopotassium phosphate, 0.6g/L dipotassium phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0g/L agar and 0.1g/L bromine bromothymol blue, wherein the pH value is 6.9-7.0. According to the vibrio chromogenic culture medium, each liter of the trisaccharide iron (TSI) agar culture-medium contains 15.0g of peptone, 3.0g of beef extract powder, 3.0g of yeast powder, 5.0g of sodium chloride, 5.0g of proteose peptone, 10.0g of lactose, 1.0g of glucose, 10.0g of cane sugar, 0.5g of ferrous sulfate, 0.1g of sodium thiosulfate, 12.0g of agar and the balance of distilled water. The culture medium provided by the invention is simple in formula, convenient to prepare and operate, low in cost and beneficial to popularize and use, and bacteria can grow rapidly and are low in aberration rate and convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of vibrio chromogenic medium.
Background technology
The ecological complexity in the variation between microorganism and variability and species habitat in the world, has brought discussion, has understood popularity, complicacy and the permanence of microorganism.Species for microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, has formed the sensitive regulation system of a set of complete unification, rely on this regulation system, strictly control various Metabolic activities.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because of culture medium prescription difference, affects its Form index situation.
Substratum (Medium) is for the nutriment of microorganism, plant and animal tissue growth and the artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single kind microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, service requirements difference, and storage and custody aspect is also slightly different.General substratum is being heated, after the moisture absorption, be easily contaminated by bacterial or decompose rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of strict sterilizing, and the storage of long period, must be placed in the refrigerator of 3～6 DEG C.Because liquid nutrient medium is difficult for long-term keeping, all change system into now powder.
Substratum is natural medium, combination substratum according to chemical classification and partly combines substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer and calculate at present the culture medium prescription of applicable a certain bacterial classification completely from the ultimate principle of biochemical reaction, can only use the basic theories of biological chemistry, cytobiology, microbiology etc., the empirical formula that is applicable to a certain class bacterial classification using with reference to forefathers, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipments such as shaking flask, glass pot, select comparatively applicable substratum according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider while determining according to forefathers' experience and medium component, tentatively determine possible medium component
Test and finally determine the most suitable medium component by single-factor
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These are tested often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, and a kind of vibrio chromogenic medium is provided.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, to the row filter that is combined into of all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulant, the materialization factors such as pH value to substratum, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding compare, and have worked out substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of vibrio chromogenic medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0;
On the basis of above scheme, described vibrio chromogenic medium, described triple sugariron (TSI) nutrient agar is in every liter of triple sugariron (TSI) nutrient agar, to contain peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, month show peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.5g, Sulfothiorine 0.1g, agar 12.0g, and all the other are distilled water.
Take this product 18.46g and add 200ml distilled water, heated and boiled is dissolved and is not stopped to stir, heating 10-20 minute.While being cooled to 45-50 DEG C, the aseptic plate of impouring.
Attention: the substratum preparing must keep surface drying, can be in the incubator of 37 DEG C upside down 1-2 hour.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of vibrio chromogenic medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0;
On the basis of above scheme, described vibrio chromogenic medium, described triple sugariron (TSI) nutrient agar is in every liter of triple sugariron (TSI) nutrient agar, to contain peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, month show peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.5g, Sulfothiorine 0.1g, agar 12.0g, and all the other are distilled water.
Take this product 18.46g and add 200ml distilled water, heated and boiled is dissolved and is not stopped to stir, heating 10-20 minute.While being cooled to 45-50 DEG C, the aseptic plate of impouring.
Attention: the substratum preparing must keep surface drying, can be in the incubator of 37 DEG C upside down 1-2 hour.
。
Claims (2)
1. a vibrio chromogenic medium, the component that it is characterized in that described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0.
2. vibrio chromogenic medium according to claim 1, it is characterized in that described triple sugariron (TSI) nutrient agar is in every liter of triple sugariron (TSI) nutrient agar, to contain peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, month show peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.5g, Sulfothiorine 0.1g, agar 12.0g, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410442803.2A CN104195213A (en) | 2014-09-02 | 2014-09-02 | Vibrio chromogenic culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410442803.2A CN104195213A (en) | 2014-09-02 | 2014-09-02 | Vibrio chromogenic culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104195213A true CN104195213A (en) | 2014-12-10 |
Family
ID=52080577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410442803.2A Pending CN104195213A (en) | 2014-09-02 | 2014-09-02 | Vibrio chromogenic culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104195213A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694433A (en) * | 2015-03-13 | 2015-06-10 | 黑龙江出入境检验检疫局检验检疫技术中心 | Culture medium capable of synchronously performing proliferation on a variety of pathogenic vibrio bacteria in aquatic product and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
| CN104651454A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Vibrio chromogenic medium |
-
2014
- 2014-09-02 CN CN201410442803.2A patent/CN104195213A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
| CN104651454A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Vibrio chromogenic medium |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694433A (en) * | 2015-03-13 | 2015-06-10 | 黑龙江出入境检验检疫局检验检疫技术中心 | Culture medium capable of synchronously performing proliferation on a variety of pathogenic vibrio bacteria in aquatic product and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Matsudo et al. | Photosynthetic efficiency and rate of CO2 assimilation by Arthrospira (Spirulina) platensis continuously cultivated in a tubular photobioreactor | |
| Brand et al. | Maintenance of microalgae in culture collections | |
| CN104195214A (en) | Listeriosis chromogenic medium | |
| CN104651467A (en) | Staphylococcus aureus chromogenic medium | |
| CN104651247A (en) | Enterobacter sakazakii chromogenic medium | |
| CN104195213A (en) | Vibrio chromogenic culture medium | |
| CN104195217A (en) | Staphylococcus aureus chromogenic culture media | |
| CN104651451A (en) | Escherichia coli chromogenic medium | |
| CN104651259A (en) | Eosin-methylene blue agar and use thereof | |
| CN104651466A (en) | Simmons citrate agar medium and use thereof | |
| CN104313115A (en) | Improved tomato juice culture medium and application thereof | |
| CN104651455A (en) | Salmonella chromogenic medium | |
| CN104263806A (en) | Fuchsin basic sodium sulfite agar and application thereof | |
| CN104651454A (en) | Vibrio chromogenic medium | |
| CN104212877A (en) | Psychrophile counting agar medium and application thereof | |
| CN104313108A (en) | Lactose bile salt fermentation culture medium and application thereof | |
| CN104263676A (en) | Simmons citrate agar and application thereof | |
| CN104195088A (en) | Chromogenic culture medium for enterobacter sakazakii | |
| CN104277972A (en) | Improved sorbitol macconkey agar culture medium | |
| CN104651249A (en) | Candida chromogenic medium and use thereof | |
| CN104651447A (en) | Lactobacillus selective agar medium and use thereof | |
| CN104651445A (en) | Urethra positioning chromogenic medium and use thereof | |
| CN104651463A (en) | Fuchsin sulfite agar and use thereof | |
| CN104278075A (en) | Escherichia coli chromogenic culture medium | |
| CN104651444A (en) | Brilliant green lactic acid medium and use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141210 |