CN104651247A - Enterobacter sakazakii chromogenic medium - Google Patents
Enterobacter sakazakii chromogenic medium Download PDFInfo
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- CN104651247A CN104651247A CN201310599335.5A CN201310599335A CN104651247A CN 104651247 A CN104651247 A CN 104651247A CN 201310599335 A CN201310599335 A CN 201310599335A CN 104651247 A CN104651247 A CN 104651247A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention belongs to the field of microbes and relates to an enterobacter sakazakii chromogenic medium. The enterobacter sakazakii chromogenic medium comprises a trisaccharide iron (TSI) medium, 1.0g/L of ammonium ferric citrate, 2.0g/L of sodium thiosulfate, 2.0g/L of sodium deoxycholate, 0.5g/L of monopotassium phosphate, 0.2g/L of 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, 15.0g/L of agar and 0.1g/L of a color developing agent. The enterobacter sakazakii chromogenic medium has a pH value of 7.1-7.5. Each liter of the TSI medium comprises 15.0g of tryptone, 5.0g of soybean peptone, 3.0g of beef extract powder, 3.0g of yeast powder, 5.0g of sodium chloride, 1.0g of glucose, 10.0g of cane sugar, 0.2g of ferrous sulphate, 0.3g of sodium hyposulfite, 12.0g of agar, 0.04g of bromcresol purple and the balance distilled water. The enterobacter sakazakii chromogenic medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of Enterobacter sakazaii colour development culture medium.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of Enterobacter sakazaii colour development culture medium.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of Enterobacter sakazaii colour development culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles of the bromo-4-of 0.2g/L5--α-D-pyranoglucose glucoside, 15.0 g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is that all the other are distilled water containing Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in often liter of triple sugariron (TSI) nutrient agar.
Take this product 8.62g and add 200ml distilled water, heated and boiled is dissolved completely, 121 DEG C of autoclaving 15min, when being chilled to about 50 DEG C, and impouring sterilized petri dishes.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of Enterobacter sakazaii colour development culture medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles of the bromo-4-of 0.2g/L5--α-D-pyranoglucose glucoside, 15.0 g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is that all the other are distilled water containing Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in often liter of triple sugariron (TSI) nutrient agar.
Take this product 8.62g and add 200ml distilled water, heated and boiled is dissolved completely, 121 DEG C of autoclaving 15min, when being chilled to about 50 DEG C, and impouring sterilized petri dishes.
The flat board prepared can preserve 2-5 days, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, and storage temperature 2-8 DEG C, notes keeping in Dark Place.
| Quality-control strains | Growing state | Colony colour |
| Enterobacter sakazakii | +++ | Blue-green |
| Streptococcus aureus | - | - |
| Salmonellas | +++ | Colourless have black center |
| Intestinal bacteria | +++ | Faint yellow |
| Klebsiella pneumonia | +++ | Colourless |
Claims (1)
1. an Enterobacter sakazaii colour development culture medium, it is characterized in that the component of described substratum comprises triple sugariron (TSI) nutrient agar, 1.0g/L ferric ammonium citrate, 2.0g/L Sulfothiorine, 2.0g/L Sodium desoxycholate, 0.5g/L potassium primary phosphate, the chloro-3-indoles of the bromo-4-of 0.2g/L5--α-D-pyranoglucose glucoside, 15.0 g/L agar, 0.1g/L developer, pH value is 7.1-7.5; Described triple sugariron (TSI) nutrient agar is that all the other are distilled water containing Tryptones 15.0g, soy peptone 5.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0.2 g, Sulfothiorine 0.3g, agar 12.0g, purpurum bromocresolis 0.04g in often liter of triple sugariron (TSI) nutrient agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599335.5A CN104651247A (en) | 2013-11-25 | 2013-11-25 | Enterobacter sakazakii chromogenic medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599335.5A CN104651247A (en) | 2013-11-25 | 2013-11-25 | Enterobacter sakazakii chromogenic medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651247A true CN104651247A (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310599335.5A Pending CN104651247A (en) | 2013-11-25 | 2013-11-25 | Enterobacter sakazakii chromogenic medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195088A (en) * | 2014-09-09 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | Chromogenic culture medium for enterobacter sakazakii |
| CN105861623A (en) * | 2016-04-25 | 2016-08-17 | 无锡市赛微生物技术有限公司 | Chromogenic culture medium for detecting Enterobacter sakazakii |
| CN113584122A (en) * | 2021-08-12 | 2021-11-02 | 合肥工业大学 | Liquid culture medium for resuscitating xerosis cutis and salmonella |
-
2013
- 2013-11-25 CN CN201310599335.5A patent/CN104651247A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195088A (en) * | 2014-09-09 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | Chromogenic culture medium for enterobacter sakazakii |
| CN105861623A (en) * | 2016-04-25 | 2016-08-17 | 无锡市赛微生物技术有限公司 | Chromogenic culture medium for detecting Enterobacter sakazakii |
| CN113584122A (en) * | 2021-08-12 | 2021-11-02 | 合肥工业大学 | Liquid culture medium for resuscitating xerosis cutis and salmonella |
| CN113584122B (en) * | 2021-08-12 | 2023-09-15 | 合肥工业大学 | Liquid medium for recovering dry injury from Cronobacter and Salmonella |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |