CN104195214A - Listeriosis chromogenic medium - Google Patents
Listeriosis chromogenic medium Download PDFInfo
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- CN104195214A CN104195214A CN201410461397.4A CN201410461397A CN104195214A CN 104195214 A CN104195214 A CN 104195214A CN 201410461397 A CN201410461397 A CN 201410461397A CN 104195214 A CN104195214 A CN 104195214A
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- listeriosis
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Abstract
The invention belongs to the field of microorganisms and in particular relates to a listeriosis chromogenic medium. The medium comprises the following components: bromcersol purple broth base, 1.0g/L of sodium hydrogen phosphate, 10.0g/L of glycine, 0.5g/L of lithium chloride, 2.5g/L of phenethyl alcohol, 15.0g/L of agar and 0.1g/L of chromogenic agent, wherein the pH value of the medium is 7.2-7.4; every 1L of bromcersol purple broth base contains 10.0g of tryptone, 3.0g of beef extract powder, 5.0g of sodium chloride, 0.04g of bromcresol purple and the balance of distilled water. The listeriosis chromogenic medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of listeria spp color developing culture medium.
Background technology
The ecological complexity in the variation between microorganism and variability and species habitat in the world, has brought discussion, has understood popularity, complicacy and the permanence of microorganism.Species for microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, has formed the sensitive regulation system of a set of complete unification, rely on this regulation system, strictly control various Metabolic activities.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because of culture medium prescription difference, affects its Form index situation.
Substratum (Medium) is for the nutriment of microorganism, plant and animal tissue growth and the artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single kind microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, service requirements difference, and storage and custody aspect is also slightly different.General substratum is being heated, after the moisture absorption, be easily contaminated by bacterial or decompose rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of strict sterilizing, and the storage of long period, must be placed in the refrigerator of 3～6 DEG C.Because liquid nutrient medium is difficult for long-term keeping, all change system into now powder.
Substratum is natural medium, combination substratum according to chemical classification and partly combines substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer and calculate at present the culture medium prescription of applicable a certain bacterial classification completely from the ultimate principle of biochemical reaction, can only use the basic theories of biological chemistry, cytobiology, microbiology etc., the empirical formula that is applicable to a certain class bacterial classification using with reference to forefathers, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipments such as shaking flask, glass pot, select comparatively applicable substratum according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider while determining according to forefathers' experience and medium component, tentatively determine possible medium component
Test and finally determine the most suitable medium component by single-factor
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These are tested often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, and a kind of listeria spp color developing culture medium is provided.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, to the row filter that is combined into of all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulant, the materialization factors such as pH value to substratum, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding compare, and have worked out substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of listeria spp color developing culture medium, the component of described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is in every liter of bromcresol purple broth base, to contain Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g, and all the other are distilled water.
Take basic medium 14.2g and add 180ml distilled water, heating for dissolving does not stop to stir; Separately get 1.2g listeria spp additive, add 20ml sterilized water, and add some glass spheres, failure of oscillation is moving, makes its emulsification even, by the two respectively at 121 DEG C of autoclaving 15min.While being chilled to 50 DEG C of left and right, listeria spp additive is added in basic medium, and add listeria spp fungistat 1 and 2 each 1, mix the aseptic plate of impouring.Make the thickness of substratum be about 5mm, the opaque ring of favourable like this observation.
The solid plate preparing is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-8 DEG C
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of listeria spp color developing culture medium, the component of described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is in every liter of bromcresol purple broth base, to contain Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g, and all the other are distilled water.
Take basic medium 14.2g and add 180ml distilled water, heating for dissolving does not stop to stir; Separately get 1.2g listeria spp additive, add 20ml sterilized water, and add some glass spheres, failure of oscillation is moving, makes its emulsification even, by the two respectively at 121 DEG C of autoclaving 15min.While being chilled to 50 DEG C of left and right, listeria spp additive is added in basic medium, and add listeria spp fungistat 1 and 2 each 1, mix the aseptic plate of impouring.Make the thickness of substratum be about 5mm, the opaque ring of favourable like this observation.
The solid plate preparing is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-8 DEG C
Embodiment in above-mentioned embodiment is illustrative instead of determinate; can list several embodiment according to institute's limited range; therefore do not departing from any variation and the amendment under general plotting of the present invention, made, within all should belonging to the protection domain that the present invention limits.
Claims (1)
1. a listeria spp color developing culture medium, the component that it is characterized in that described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is in every liter of bromcresol purple broth base, to contain Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g, and all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461397.4A CN104195214A (en) | 2014-09-12 | 2014-09-12 | Listeriosis chromogenic medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410461397.4A CN104195214A (en) | 2014-09-12 | 2014-09-12 | Listeriosis chromogenic medium |
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| CN104195214A true CN104195214A (en) | 2014-12-10 |
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| CN201410461397.4A Pending CN104195214A (en) | 2014-09-12 | 2014-09-12 | Listeriosis chromogenic medium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349611A (en) * | 2015-11-24 | 2016-02-24 | 湖北工业大学 | Cold hydrogel test strip for rapid detection of Listeria monocytogenes in food and tableware, and preparation method and application thereof |
| CN105506056A (en) * | 2016-02-05 | 2016-04-20 | 刘名霞 | Feces sample listeria monocytogenes culture medium |
| CN105506054A (en) * | 2016-02-05 | 2016-04-20 | 夏淑平 | Listeria monocytogenes selective medium |
| CN105506055A (en) * | 2016-02-05 | 2016-04-20 | 夏淑平 | Selective medium for listeria monocytogenes of stool sample |
| CN105543326A (en) * | 2016-02-05 | 2016-05-04 | 刘名霞 | Listerella selective culture medium |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
| CN104651443A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Listeria chromogenic medium |
-
2014
- 2014-09-12 CN CN201410461397.4A patent/CN104195214A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812423A (en) * | 2010-01-08 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Culture medium for producing synthetic probiotic groups through fermentation |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
| CN104651443A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Listeria chromogenic medium |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349611A (en) * | 2015-11-24 | 2016-02-24 | 湖北工业大学 | Cold hydrogel test strip for rapid detection of Listeria monocytogenes in food and tableware, and preparation method and application thereof |
| CN105506056A (en) * | 2016-02-05 | 2016-04-20 | 刘名霞 | Feces sample listeria monocytogenes culture medium |
| CN105506054A (en) * | 2016-02-05 | 2016-04-20 | 夏淑平 | Listeria monocytogenes selective medium |
| CN105506055A (en) * | 2016-02-05 | 2016-04-20 | 夏淑平 | Selective medium for listeria monocytogenes of stool sample |
| CN105543326A (en) * | 2016-02-05 | 2016-05-04 | 刘名霞 | Listerella selective culture medium |
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Application publication date: 20141210 |
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