CN104263676A - Simmons citrate agar and application thereof - Google Patents
Simmons citrate agar and application thereof Download PDFInfo
- Publication number
- CN104263676A CN104263676A CN201410442424.3A CN201410442424A CN104263676A CN 104263676 A CN104263676 A CN 104263676A CN 201410442424 A CN201410442424 A CN 201410442424A CN 104263676 A CN104263676 A CN 104263676A
- Authority
- CN
- China
- Prior art keywords
- agar
- citrate
- simmons
- culture medium
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the field of microorganism and particularly relates to Simmons citrate agar. A culture medium comprises the following components: EC broth, 0.2g/L magnesium sulfate, 1.0g/L ammonium dihydrogen phosphate, 5.0g/L sodium citrate, 3.0g/L manganese chloride, 15.0g/L agar and 0.08g/L bromothymol blue, wherein the pH of the medium culture is 6.7-6.9. The EC broth per litter comprises 20.0g of tryptone, 5.0g of sodium chloride, 4.0g of dipotassium phosphate and the balance of distilled water. The culture medium provided by the invention is simple in formula, convenient to prepare and operate, low in cost and favorably popularized and applied. The culture medium is fast in growth of bacteria, low in aberration rate and convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of simmons citrate agar and application thereof.
Background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer from the ultimate principle of biochemical reaction completely and calculate the culture medium prescription of applicable a certain bacterial classification at present, the basic theories of biological chemistry, cytobiology, microbiology etc. can only be used, with reference to the empirical formula being comparatively applicable to a certain class bacterial classification that forefathers use, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipment such as shaking flask, glass pot, select the substratum be comparatively applicable to according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider when determining according to the experience of forefathers and medium component, tentatively determine possible medium component
Medium component the most suitable is finally determined by single factor experiment
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These experiments, often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of simmons citrate agar and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of simmons citrate agar, the component of described substratum comprises EC meat soup, 0.2g/L magnesium sulfate, 1.0g/L primary ammonium phosphate, 5.0g/L Trisodium Citrate, 3.0g/L Manganous chloride tetrahydrate, 15.0g/L agar, 0.08g/L bromothymol blue pH value are 6.7-6.9.
Preferably, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
The invention also discloses a kind of application of simmons citrate agar, the Citrate trianion for entero-bacte utilizes test.
Take this product 27.88g and add 1000ml distilled water, do not stop stirring heating and dissolve, packing test tube, packing is pipe 10mL often, and puts into a little voltage regulator tube, and 115 DEG C of autoclaving 15min, cool rapidly, for subsequent use.
The liquid nutrient medium prepared is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-30 DEG C.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of simmons citrate agar, the component of described substratum comprises EC meat soup, 0.2g/L magnesium sulfate, 1.0g/L primary ammonium phosphate, 5.0g/L Trisodium Citrate, 3.0g/L Manganous chloride tetrahydrate, 15.0g/L agar, 0.08g/L bromothymol blue pH value are 6.7-6.9.
Described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
Take this product 27.88g and add 1000ml distilled water, do not stop stirring heating and dissolve, packing test tube, packing is pipe 10mL often, and puts into a little voltage regulator tube, and 115 DEG C of autoclaving 15min, cool rapidly, for subsequent use.
The liquid nutrient medium prepared is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-30 DEG C.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Enteroaerogen | Well | Positive, blue |
| Colon bacillus | Part suppresses | Negative, green |
| Shigella flexneri | Part suppresses | Negative, green |
Embodiment in above-mentioned embodiment is illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore do not departing from any change and amendment made under general plotting of the present invention, all should belong within protection domain that the present invention limits.
Claims (3)
1. a simmons citrate agar, is characterized in that the component of described substratum comprises EC meat soup, 0.2g/L magnesium sulfate, 1.0g/L primary ammonium phosphate, 5.0g/L Trisodium Citrate, 3.0g/L Manganous chloride tetrahydrate, 15.0g/L agar, 0.08g/L bromothymol blue pH value are 6.7-6.9.
2. a kind of simmons citrate agar according to claim 1, it is characterized in that described EC meat soup is for containing Tryptones 20.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup, all the other are distilled water.
3. an application for a kind of simmons citrate agar according to claim 1, is characterized in that the Citrate trianion for entero-bacte utilizes test.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410442424.3A CN104263676A (en) | 2014-09-02 | 2014-09-02 | Simmons citrate agar and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410442424.3A CN104263676A (en) | 2014-09-02 | 2014-09-02 | Simmons citrate agar and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104263676A true CN104263676A (en) | 2015-01-07 |
Family
ID=52155260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410442424.3A Pending CN104263676A (en) | 2014-09-02 | 2014-09-02 | Simmons citrate agar and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104263676A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733205A (en) * | 2020-08-20 | 2020-10-02 | 四川大学华西医院 | Carbapenem-resistant Klebsiella chromogenic medium |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181518A (en) * | 2010-12-29 | 2011-09-14 | 国家海洋环境监测中心 | Method for identifying marine water fecal pollution source by using genetic finger-print of Escherichia coli |
| CN104651466A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Simmons citrate agar medium and use thereof |
-
2014
- 2014-09-02 CN CN201410442424.3A patent/CN104263676A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181518A (en) * | 2010-12-29 | 2011-09-14 | 国家海洋环境监测中心 | Method for identifying marine water fecal pollution source by using genetic finger-print of Escherichia coli |
| CN104651466A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Simmons citrate agar medium and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张丹鹤等: "鸡源福氏志贺氏菌的分离鉴定及药敏试验", 《河南农业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733205A (en) * | 2020-08-20 | 2020-10-02 | 四川大学华西医院 | Carbapenem-resistant Klebsiella chromogenic medium |
| CN111733205B (en) * | 2020-08-20 | 2021-06-25 | 四川大学华西医院 | Carbapenem-resistant Klebsiella chromogenic medium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Matsudo et al. | Photosynthetic efficiency and rate of CO2 assimilation by Arthrospira (Spirulina) platensis continuously cultivated in a tubular photobioreactor | |
| CN104195214A (en) | Listeriosis chromogenic medium | |
| CN104651466A (en) | Simmons citrate agar medium and use thereof | |
| CN104651467A (en) | Staphylococcus aureus chromogenic medium | |
| CN104651247A (en) | Enterobacter sakazakii chromogenic medium | |
| CN104263676A (en) | Simmons citrate agar and application thereof | |
| CN104651259A (en) | Eosin-methylene blue agar and use thereof | |
| CN104651451A (en) | Escherichia coli chromogenic medium | |
| CN104651455A (en) | Salmonella chromogenic medium | |
| CN104195217A (en) | Staphylococcus aureus chromogenic culture media | |
| CN104212877A (en) | Psychrophile counting agar medium and application thereof | |
| CN104313108A (en) | Lactose bile salt fermentation culture medium and application thereof | |
| CN104263806A (en) | Fuchsin basic sodium sulfite agar and application thereof | |
| CN104277972A (en) | Improved sorbitol macconkey agar culture medium | |
| CN104313115A (en) | Improved tomato juice culture medium and application thereof | |
| CN104278075A (en) | Escherichia coli chromogenic culture medium | |
| CN104651450A (en) | Lactose-bile salt fermentation medium and use thereof | |
| CN104651444A (en) | Brilliant green lactic acid medium and use thereof | |
| CN104651447A (en) | Lactobacillus selective agar medium and use thereof | |
| CN104651252A (en) | Deoxycholate hydrogen sulfide lactose agar medium and use thereof | |
| CN104651463A (en) | Fuchsin sulfite agar and use thereof | |
| CN104195213A (en) | Vibrio chromogenic culture medium | |
| CN104651442A (en) | Crystal violet-neutral red bile salt agar and use thereof | |
| CN104651464A (en) | Deoxycholate agar medium and use thereof | |
| CN104293709A (en) | Eosin-methylene blue agar and applications thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150107 |
|
| WD01 | Invention patent application deemed withdrawn after publication |