CN104651442A - Crystal violet-neutral red bile salt agar and use thereof - Google Patents
Crystal violet-neutral red bile salt agar and use thereof Download PDFInfo
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- CN104651442A CN104651442A CN201310599322.8A CN201310599322A CN104651442A CN 104651442 A CN104651442 A CN 104651442A CN 201310599322 A CN201310599322 A CN 201310599322A CN 104651442 A CN104651442 A CN 104651442A
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- crystal violet
- agar
- bile salt
- neutral red
- red bile
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the field of microbes and relates to crystal violet-neutral red bile salt agar and a use thereof. The crystal violet-neutral red bile salt agar comprises EC broth, 3.0g/L of yeast powder, 0.002g/L of crystal violet, 0.03g/L of neutral red, 15.0g/L of agar and 3.0g/L of dipotassium hydrogen phosphate. The crystal violet-neutral red bile salt agar has a pH value of 7.3-7.5. Each liter of the EC broth comprises 20.0g of tryptone, 5.0g of lactose, 2.0 g of potassium dihydrogen phosphate, 5.0g of common salt, 1.5g of bile salt NO.3, 4.0g of dipotassium hydrogen phosphate and the balance of distilled water. The crystal violet-neutral red bile salt agar has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of violet red bile agar and application thereof.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of violet red bile agar and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of violet red bile agar, the component of described substratum comprises EC meat soup, 3.0g/L yeast powder, 0.002g/L Viola crystallina, 0.03g/L toluylene red, 15.0 g/L agar, 3.0g/L dipotassium hydrogen phosphate, pH value are 7.3-7.5;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 5.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
The invention also discloses described violet red bile agar, the solid plate for coliform detects.
Take this product 41.5g, heating for dissolving, in 1000ml distilled water, is boiled and is not exceeded 2 minutes.Get acceptable diluent degree sample liquid 1ml, join sterilized petri dishes center, the VRBA10-15ml being chilled to 45 ± 0.5 DEG C is poured in plate.Substratum and sample liquid fully mix by careful rotation plate.After solidifying, then add 3-4mlVRBA and cover dull and stereotyped top layer, upset is dull and stereotyped, is placed in 36 ± 1 DEG C and cultivates 18-24 hour.Without the need to autoclaving.Face used time preparation, must not more than 3 hours.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of violet red bile agar, the component of described substratum comprises EC meat soup, 3.0g/L yeast powder, 0.002g/L Viola crystallina, 0.03g/L toluylene red, 15.0 g/L agar, 3.0g/L dipotassium hydrogen phosphate, pH value are 7.3-7.5;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 5.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
The invention also discloses described violet red bile agar, the solid plate for coliform detects.
Take this product 41.5g, heating for dissolving, in 1000ml distilled water, is boiled and is not exceeded 2 minutes.Get acceptable diluent degree sample liquid 1ml, join sterilized petri dishes center, the VRBA10-15ml being chilled to 45 ± 0.5 DEG C is poured in plate.Substratum and sample liquid fully mix by careful rotation plate.After solidifying, then add 3-4mlVRBA and cover dull and stereotyped top layer, upset is dull and stereotyped, is placed in 36 ± 1 DEG C and cultivates 18-24 hour.Without the need to autoclaving.Face used time preparation, must not more than 3 hours.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | - | / |
| Colon bacillus | +++ | Red-purple bacterium colony, has red precipitate ring around |
| Enterobacter cloacae | +++ | Red-purple bacterium colony, has red precipitate ring around |
| Salmonellas | +++ | Colony colourless is transparent |
Claims (2)
1. a violet red bile agar, is characterized in that the component of described substratum comprises EC meat soup, 3.0g/L yeast powder, 0.002g/L Viola crystallina, 0.03g/L toluylene red, 15.0 g/L agar, 3.0g/L dipotassium hydrogen phosphate, pH value are 7.3-7.5;
Violet red bile agar according to claim 1, it is characterized in that described EC meat soup is for containing Tryptones 20.0g, lactose 5.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, no. 3 bile salt 1.5g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup, all the other are distilled water.
2. a violet red bile agar according to claim 1, is characterized in that the solid plate for coliform detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599322.8A CN104651442A (en) | 2013-11-25 | 2013-11-25 | Crystal violet-neutral red bile salt agar and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599322.8A CN104651442A (en) | 2013-11-25 | 2013-11-25 | Crystal violet-neutral red bile salt agar and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651442A true CN104651442A (en) | 2015-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201310599322.8A Pending CN104651442A (en) | 2013-11-25 | 2013-11-25 | Crystal violet-neutral red bile salt agar and use thereof |
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| CN (1) | CN104651442A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669707A (en) * | 2019-11-27 | 2020-01-10 | 中秀科技股份有限公司 | Mackanka agar plate and preparation method thereof |
-
2013
- 2013-11-25 CN CN201310599322.8A patent/CN104651442A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110669707A (en) * | 2019-11-27 | 2020-01-10 | 中秀科技股份有限公司 | Mackanka agar plate and preparation method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |