CN104651454A - Vibrio chromogenic medium - Google Patents
Vibrio chromogenic medium Download PDFInfo
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- CN104651454A CN104651454A CN201310599829.3A CN201310599829A CN104651454A CN 104651454 A CN104651454 A CN 104651454A CN 201310599829 A CN201310599829 A CN 201310599829A CN 104651454 A CN104651454 A CN 104651454A
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- agar
- vibrio
- tsi
- medium
- chromogenic medium
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the field of microbes and especially relates to a vibrio chromogenic medium. The vibrio chromogenic medium comprises a trisaccharide iron (TSI) agar medium, 0.5g/L of disodium hydrogen phosphate, 0.4g/L of potassium dihydrogen phosphate, 0.6g/L of dipotassium hydrogen phosphate, 3.0g/L of sodium malonate, 2.5g/L of ammonium sulfate, 15.0g/L of agar and 0.1g/L of bromothymol blue. The vibrio chromogenic medium has a pH value of 6.9-7.0. Each liter of the TSI agar medium comprises 15.0g of tryptone, 3.0g of beef extract powder, 3.0g of yeast powder, 5.0g of sodium chloride, 5.0g of proteose peptone, 10.0g of glucose, 1.0g/L of glucose, 10.0g of cane sugar, 0.5g of ferrous sulphate, 0.1g of sodium hyposulfite, 12.0g of agar and the balance distilled water. The vibrio chromogenic medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of vibrio chromogenic medium.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of vibrio chromogenic medium.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of vibrio chromogenic medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0 g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0;
On the basis of above scheme, described vibrio chromogenic medium, described triple sugariron (TSI) nutrient agar is for containing peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0. 5g, Sulfothiorine 0.1g, agar 12.0g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Take this product 18.46g and add 200ml distilled water, heated and boiled is dissolved and is not stopped to stir, heating 10-20 minute.When being cooled to 45-50 DEG C, impouring sterilized petri dishes.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of vibrio chromogenic medium, the component of described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0 g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0;
On the basis of above scheme, described vibrio chromogenic medium, described triple sugariron (TSI) nutrient agar is for containing peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0. 5g, Sulfothiorine 0.1g, agar 12.0g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Take this product 18.46g and add 200ml distilled water, heated and boiled is dissolved and is not stopped to stir, heating 10-20 minute.When being cooled to 45-50 DEG C, impouring sterilized petri dishes.
Attention: the substratum prepared must keep surface drying, can in the incubator of 37 DEG C upside down 1-2 hour.
| Quality-control strains | Growing state | Colony colour |
| Vibrio parahemolyticus | +++ | Blue to blue-greenish colour |
| Streptococcus aureus | - | - |
| Intestinal bacteria | -/+ | Colourless |
| Vibrio alginolyticus | +++ | Colourless |
| Vibrio cholerae | +++ | Colourless |
Claims (2)
1. a vibrio chromogenic medium, it is characterized in that the component of described substratum comprises triple sugariron (TSI) nutrient agar, 0.5g/L Sodium phosphate dibasic, 0.4g/L potassium primary phosphate, 0.6g/L dipotassium hydrogen phosphate, 3.0g/L sodium malonate, 2.5g/L ammonium sulfate, 15.0 g/L agar, 0.1g/L bromothymol blue, pH value is 6.9-7.0.
2. vibrio chromogenic medium according to claim 1, it is characterized in that described triple sugariron (TSI) nutrient agar is for containing peptone 15.0g, beef extract powder 3.0g, yeast powder 3.0g, sodium-chlor 5.0g, showing peptone 5.0g, lactose 10.0g, glucose 1.0g, sucrose 10.0g, ferrous sulfate 0. 5g, Sulfothiorine 0.1g, agar 12.0g the moon in often liter of triple sugariron (TSI) nutrient agar, all the other are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599829.3A CN104651454A (en) | 2013-11-25 | 2013-11-25 | Vibrio chromogenic medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599829.3A CN104651454A (en) | 2013-11-25 | 2013-11-25 | Vibrio chromogenic medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651454A true CN104651454A (en) | 2015-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201310599829.3A Pending CN104651454A (en) | 2013-11-25 | 2013-11-25 | Vibrio chromogenic medium |
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| CN (1) | CN104651454A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195213A (en) * | 2014-09-02 | 2014-12-10 | 青岛永通电梯工程有限公司 | Vibrio chromogenic culture medium |
-
2013
- 2013-11-25 CN CN201310599829.3A patent/CN104651454A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195213A (en) * | 2014-09-02 | 2014-12-10 | 青岛永通电梯工程有限公司 | Vibrio chromogenic culture medium |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |