CN104263806A - Fuchsin basic sodium sulfite agar and application thereof - Google Patents
Fuchsin basic sodium sulfite agar and application thereof Download PDFInfo
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- CN104263806A CN104263806A CN201410512982.2A CN201410512982A CN104263806A CN 104263806 A CN104263806 A CN 104263806A CN 201410512982 A CN201410512982 A CN 201410512982A CN 104263806 A CN104263806 A CN 104263806A
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- sodium sulfite
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- basic sodium
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Abstract
The invention relates to the field of microorganisms, and particularly relates to fuchsin basic sodium sulfite agar. A culture medium comprises the following components: EC broth, 5.0g/L of beef powder, 5.0g/L of yeast powder, 1.0g/L of basic fuchsin, 6.0g/L of sodium sulfite and 15.0g/L of agar; the pH value is 7.2-7.4; and each liter of EC broth contains 20.0g of tryptone, 10.0g of lactose, 2.0g of monopotassium phosphate, 5.0g of sodium chloride, 4.0g of dipotassium phosphate and the balance of distilled water. The culture medium disclosed by the invention is simple in component, convenient to prepare and operate, low in cost, and beneficial for popularization and application; and bacteria are rapid in growth, low in aberration rate, and convenient to observe and analyze.
Description
Technical field
The present invention is microorganism field, particularly a kind of fuchsin basic sodium sulfite agar and application thereof.
Background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Can't infer from the ultimate principle of biochemical reaction completely and calculate the culture medium prescription of applicable a certain bacterial classification at present, the basic theories of biological chemistry, cytobiology, microbiology etc. can only be used, with reference to the empirical formula being comparatively applicable to a certain class bacterial classification that forefathers use, again in conjunction with the characteristic of bacterial classification used and product, adopt the small-sized fermentation equipment such as shaking flask, glass pot, select the substratum be comparatively applicable to according to certain experimental design and experimental technique.
The basic step of substratum design is:
Some problems that must consider when determining according to the experience of forefathers and medium component, tentatively determine possible medium component
Medium component the most suitable is finally determined by single factor experiment
After medium component is determined, remaining issues is exactly the suitableeest concentration of each composition, because medium component is a lot, often adopts some rational experimental design methods for reducing experiment number.These experiments, often based on factorial experiment, comprise homogeneous design, orthogonal, response surface analysis etc.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of fuchsin basic sodium sulfite agar and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of fuchsin basic sodium sulfite agar, the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, and pH value is 7.2-7.4;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
The invention also discloses the application of described fuchsin basic sodium sulfite agar, for tap water, the selective separation of total coli group and confirmation in source water.
Take this product 52.5g, add 20ml dehydrated alcohol and 980ml distilled water, heated and stirred is dissolved, packing, and 116 DEG C of autoclavings 20 minutes are for subsequent use.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of fuchsin basic sodium sulfite agar, the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, and pH value is 7.2-7.4;
On the basis of above scheme, described EC meat soup is that all the other are distilled water containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup.
Take this product 52.5g, add 20ml dehydrated alcohol and 980ml distilled water, heated and stirred is dissolved, packing, and 116 DEG C of autoclavings 20 minutes are for subsequent use.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state | Further feature |
| Streptococcus aureus | - | - |
| Colon bacillus | +++ | Red-purple bacterium colony, there is metalluster |
| Salmonellas | +++ | Colony colourless is transparent |
Embodiment in above-mentioned embodiment is illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore do not departing from any change and amendment made under general plotting of the present invention, all should belong within protection domain that the present invention limits.
Claims (3)
1. a fuchsin basic sodium sulfite agar, it is characterized in that the component of described substratum comprises EC meat soup, 5.0g/L beef powder, 5.0g/L yeast powder, 1.0g/L magenta, 6.0g/L S-WAT, 15.0g/L agar, pH value is 7.2-7.4.
2. fuchsin basic sodium sulfite agar according to claim 1, it is characterized in that described EC meat soup is for containing Tryptones 20.0g, lactose 10.0g, potassium primary phosphate 2.0g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 4.0g in often liter of EC meat soup, all the other are distilled water.
3. an application for fuchsin basic sodium sulfite agar according to claim 1, is characterized in that for tap water, the selective separation of total coli group and confirmation in source water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410512982.2A CN104263806A (en) | 2014-09-29 | 2014-09-29 | Fuchsin basic sodium sulfite agar and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410512982.2A CN104263806A (en) | 2014-09-29 | 2014-09-29 | Fuchsin basic sodium sulfite agar and application thereof |
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| CN104263806A true CN104263806A (en) | 2015-01-07 |
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| CN201410512982.2A Pending CN104263806A (en) | 2014-09-29 | 2014-09-29 | Fuchsin basic sodium sulfite agar and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200134915A (en) * | 2019-05-24 | 2020-12-02 | 기산바이오 주식회사 | Culture media kit for testing of quality process and method of improving preservation period of media thereof |
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| CN102181518A (en) * | 2010-12-29 | 2011-09-14 | 国家海洋环境监测中心 | Method for identifying marine water fecal pollution source by using genetic finger-print of Escherichia coli |
| CN103642891A (en) * | 2013-11-29 | 2014-03-19 | 中山鼎晟生物科技有限公司 | Method for inspecting microbes in cosmetics |
| CN103667417A (en) * | 2013-12-05 | 2014-03-26 | 中山奈德生物科技有限公司 | Method for detecting microbes in beverage |
| CN104651463A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Fuchsin sulfite agar and use thereof |
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2014
- 2014-09-29 CN CN201410512982.2A patent/CN104263806A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102181518A (en) * | 2010-12-29 | 2011-09-14 | 国家海洋环境监测中心 | Method for identifying marine water fecal pollution source by using genetic finger-print of Escherichia coli |
| CN104651463A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Fuchsin sulfite agar and use thereof |
| CN103642891A (en) * | 2013-11-29 | 2014-03-19 | 中山鼎晟生物科技有限公司 | Method for inspecting microbes in cosmetics |
| CN103667417A (en) * | 2013-12-05 | 2014-03-26 | 中山奈德生物科技有限公司 | Method for detecting microbes in beverage |
Non-Patent Citations (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200134915A (en) * | 2019-05-24 | 2020-12-02 | 기산바이오 주식회사 | Culture media kit for testing of quality process and method of improving preservation period of media thereof |
| KR102195078B1 (en) | 2019-05-24 | 2020-12-24 | 기산바이오(주) | Culture media kit for testing of quality process and method of improving preservation period of media thereof |
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