CN104651443A - Listeria chromogenic medium - Google Patents
Listeria chromogenic medium Download PDFInfo
- Publication number
- CN104651443A CN104651443A CN201310599325.1A CN201310599325A CN104651443A CN 104651443 A CN104651443 A CN 104651443A CN 201310599325 A CN201310599325 A CN 201310599325A CN 104651443 A CN104651443 A CN 104651443A
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- China
- Prior art keywords
- listeria
- bromcresol purple
- chromogenic medium
- broth base
- medium
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Abstract
The invention relates to the field of microbes and especially relates to a listeria chromogenic medium. The listeria chromogenic medium comprises bromcresol purple broth base, 1.0g/L of disodium hydrogen phosphate, 10.0g/L of glycine, 0.5g/L of lithium chloride, 2.5g/L of phenylethyl alcohol, 15.0g/L of agar and 0.1g/L of a color developing agent. The listeria chromogenic medium has a pH value of 7.2-7.4. Each liter of the bromcresol purple broth base comprises 10.0g of tryptone, 3.0g of beef extract powder, 5.0g of common salt, 0.04g of bromcresol purple and the balance distilled water. The listeria chromogenic medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.
Description
technical field
The present invention is microorganism field, particularly a kind of listeria spp color developing culture medium.
background technology
The ecological complexity in the variation in the world between microorganism and variability and species habitat, brings discussion, the popularity understanding microorganism, complicacy and permanence.The species of microorganism are exactly a unique gene pool, the metabolism of microorganism is complicated vital movement, in long-term organic evolution, defines the sensitive regulation system that complete set is unified, rely on this regulation system, the various Metabolic activity of strict control.Coordinate without any confusion to carry out and flexible adaptation environment.Different culture media, because culture medium prescription is different, affects its Form index situation.
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
the present invention overcomes deficiency of the prior art, provides a kind of listeria spp color developing culture medium.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of listeria spp color developing culture medium, the component of described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
Take basic medium 14.2g and add 180ml distilled water, heating for dissolving does not stop to stir; Separately get 1.2g Listeria bacteria additive, add 20ml sterilized water, and add some glass spheres, failure of oscillation does not move, and makes its emulsification even, by the two respectively at 121 DEG C of autoclaving 15 min.When being chilled to about 50 DEG C, Listeria bacteria additive is added in basic medium, and add each 1 of listeria spp fungistat 1 and 2, mixing, impouring sterilized petri dishes.The thickness of substratum is made to be about 5mm, the opaque ring of favourable like this observation.
The solid plate prepared is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-8 DEG C
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
embodiment
Embodiment 1:
A kind of listeria spp color developing culture medium, the component of described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
Take basic medium 14.2g and add 180ml distilled water, heating for dissolving does not stop to stir; Separately get 1.2g Listeria bacteria additive, add 20ml sterilized water, and add some glass spheres, failure of oscillation does not move, and makes its emulsification even, by the two respectively at 121 DEG C of autoclaving 15 min.When being chilled to about 50 DEG C, Listeria bacteria additive is added in basic medium, and add each 1 of listeria spp fungistat 1 and 2, mixing, impouring sterilized petri dishes.The thickness of substratum is made to be about 5mm, the opaque ring of favourable like this observation.
The solid plate prepared is stored in 2-8 DEG C, should avoid light direct irradiation.Dehydrated medium should be positioned over dark dry place, storage temperature 2-8 DEG C.
Claims (1)
1. a listeria spp color developing culture medium, it is characterized in that the component of described substratum comprises bromcresol purple broth base, 1.0g/L Sodium phosphate dibasic, 10.0g/L glycine, 0.5g/L lithium chloride, 2.5g/L phenylethyl alcohol, 15.0 g/L agar, 0.1g developer, pH value is 7.2-7.4; Described bromcresol purple broth base is that all the other are distilled water containing Tryptones 10.0g, beef extract powder 3.0g, sodium-chlor 5.0g, purpurum bromocresolis 0.04g in often liter of bromcresol purple broth base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599325.1A CN104651443A (en) | 2013-11-25 | 2013-11-25 | Listeria chromogenic medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310599325.1A CN104651443A (en) | 2013-11-25 | 2013-11-25 | Listeria chromogenic medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104651443A true CN104651443A (en) | 2015-05-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310599325.1A Pending CN104651443A (en) | 2013-11-25 | 2013-11-25 | Listeria chromogenic medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104651443A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195214A (en) * | 2014-09-12 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | Listeriosis chromogenic medium |
-
2013
- 2013-11-25 CN CN201310599325.1A patent/CN104651443A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195214A (en) * | 2014-09-12 | 2014-12-10 | 青岛润鑫伟业科贸有限公司 | Listeriosis chromogenic medium |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150527 |