CN104145029B - 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 - Google Patents
利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320413.7A CN106011234B (zh) | 2012-02-02 | 2012-07-03 | 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20120010681 | 2012-02-02 | ||
| KR10-2012-0010681 | 2012-02-02 | ||
| KR10-2012-0028429 | 2012-03-20 | ||
| KR1020120028429A KR20130101952A (ko) | 2012-02-02 | 2012-03-20 | Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
| PCT/KR2012/005281 WO2013115442A1 (en) | 2012-02-02 | 2012-07-03 | Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide hybridization assay |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610320413.7A Division CN106011234B (zh) | 2012-02-02 | 2012-07-03 | 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104145029A CN104145029A (zh) | 2014-11-12 |
| CN104145029B true CN104145029B (zh) | 2016-06-01 |
Family
ID=49451920
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280071069.2A Active CN104145029B (zh) | 2012-02-02 | 2012-07-03 | 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 |
| CN201610320413.7A Active CN106011234B (zh) | 2012-02-02 | 2012-07-03 | 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610320413.7A Active CN106011234B (zh) | 2012-02-02 | 2012-07-03 | 利用探测和标记寡核苷酸切割及延伸-依赖性信号传导寡核苷酸杂交的靶核酸序列的检测 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US9868980B2 (https=) |
| EP (1) | EP2809797B1 (https=) |
| JP (1) | JP6005180B2 (https=) |
| KR (2) | KR20130101952A (https=) |
| CN (2) | CN104145029B (https=) |
| AU (2) | AU2012368436B2 (https=) |
| BR (1) | BR112014018940B1 (https=) |
| CA (1) | CA2862488C (https=) |
| IL (1) | IL233773B (https=) |
| MX (1) | MX361903B (https=) |
| NO (1) | NO20140948A1 (https=) |
| RU (1) | RU2608501C2 (https=) |
| SG (1) | SG11201404349YA (https=) |
| WO (1) | WO2013115442A1 (https=) |
| ZA (1) | ZA201405463B (https=) |
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| JP5852222B2 (ja) | 2011-03-29 | 2016-02-03 | シージーン アイエヌシー | Ｐｔｏ切断及び延長−依存的切断によるターゲット核酸配列の検出 |
| MX342067B (es) | 2011-05-04 | 2016-09-09 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo por desdoblamiento e hibridización de oligonucleótido de sonda. |
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| EP2823061B1 (en) | 2012-03-05 | 2018-02-14 | Seegene, Inc. | Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay |
| WO2013157821A1 (en) | 2012-04-19 | 2013-10-24 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage |
| KR101572682B1 (ko) | 2013-05-30 | 2015-11-30 | 케이맥(주) | 바이오 물질의 실시간 정량 및 정성 분석 방법 |
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