DK3237636T3 - Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer - Google Patents
Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer Download PDFInfo
- Publication number
- DK3237636T3 DK3237636T3 DK15820430.5T DK15820430T DK3237636T3 DK 3237636 T3 DK3237636 T3 DK 3237636T3 DK 15820430 T DK15820430 T DK 15820430T DK 3237636 T3 DK3237636 T3 DK 3237636T3
- Authority
- DK
- Denmark
- Prior art keywords
- double
- nucleic acids
- target nucleic
- multiplex detection
- quenching analysis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA201470813 | 2014-12-22 | ||
| PCT/DK2015/050412 WO2016101959A1 (en) | 2014-12-22 | 2015-12-22 | Dual quenching assay for multiplex detection of target nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3237636T3 true DK3237636T3 (da) | 2021-01-25 |
Family
ID=55070628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15820430.5T DK3237636T3 (da) | 2014-12-22 | 2015-12-22 | Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20180355413A1 (da) |
| EP (1) | EP3237636B1 (da) |
| JP (1) | JP2018500932A (da) |
| CN (1) | CN107109492B (da) |
| CA (1) | CA2970966A1 (da) |
| DK (1) | DK3237636T3 (da) |
| WO (1) | WO2016101959A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102110985B1 (ko) * | 2015-12-15 | 2020-05-14 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 시그널 추출 |
| KR102468174B1 (ko) * | 2016-09-15 | 2022-11-17 | 에프. 호프만-라 로슈 아게 | 멀티플렉스 pcr 수행 방법 |
| CA3088250A1 (en) * | 2018-01-22 | 2019-07-25 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| CN112592964A (zh) * | 2020-12-17 | 2021-04-02 | 厦门大学 | 用于进行核酸多重检测的方法 |
| WO2023043280A1 (ko) | 2021-09-17 | 2023-03-23 | 주식회사 씨젠 | 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출 |
| CN113846187B (zh) * | 2021-10-24 | 2023-07-18 | 北京宏微特斯生物科技有限公司 | 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| DE69829760T3 (de) | 1997-09-12 | 2016-04-14 | Exiqon A/S | Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga |
| US7824859B2 (en) * | 1999-10-29 | 2010-11-02 | Cytyc Corporation | Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase |
| US7534568B2 (en) * | 1999-10-29 | 2009-05-19 | Hologic Inc. | Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe |
| US7585632B2 (en) * | 1999-10-29 | 2009-09-08 | Hologic, Inc. | Compositions and methods for the detection of a nucleic acid using a cleavage reaction |
| US7829313B2 (en) * | 2000-03-24 | 2010-11-09 | Eppendorf Array Technologies | Identification and quantification of a plurality of biological (micro)organisms or their components |
| EP1509624A2 (en) * | 2002-05-31 | 2005-03-02 | Secretary, Department Of Atomic Energy | Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands |
| WO2005010199A2 (en) * | 2003-07-16 | 2005-02-03 | Geneohm Sciences, Inc. | Invasive cleavage reaction with electrochemical readout |
| SI1888748T1 (sl) | 2005-05-25 | 2014-02-28 | Tina Holding Aps | Stabilna in selektivna formacija hoogsteen-tipa tripleksov in dupleksov, ki uporabljajo zvite interkalatne nukleinske kisline (TINA) in postopek za pripravo TINA |
| EP2014774A1 (en) | 2007-07-11 | 2009-01-14 | Pathofinder B.V. | Assay for the simulataneous detection of multiple nucleic acid sequences in a sample |
| ES2755934T3 (es) * | 2011-10-31 | 2020-04-24 | Eiken Chemical | Método para detectar ácido nucleico diana |
| KR20130101952A (ko) | 2012-02-02 | 2013-09-16 | 주식회사 씨젠 | Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출 |
| AU2013228226B2 (en) * | 2012-03-05 | 2016-06-02 | Seegene, Inc. | Detection of nucleotide variation on target nucleic acid sequence by PTO cleavage and extension assay |
| US20140038182A1 (en) * | 2012-07-17 | 2014-02-06 | Dna Logix, Inc. | Cooperative primers, probes, and applications thereof |
-
2015
- 2015-12-22 CA CA2970966A patent/CA2970966A1/en not_active Abandoned
- 2015-12-22 DK DK15820430.5T patent/DK3237636T3/da active
- 2015-12-22 CN CN201580071965.2A patent/CN107109492B/zh active Active
- 2015-12-22 EP EP15820430.5A patent/EP3237636B1/en active Active
- 2015-12-22 JP JP2017550980A patent/JP2018500932A/ja active Pending
- 2015-12-22 US US15/535,906 patent/US20180355413A1/en not_active Abandoned
- 2015-12-22 WO PCT/DK2015/050412 patent/WO2016101959A1/en active Application Filing
-
2021
- 2021-02-24 US US17/183,824 patent/US20210189468A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20180355413A1 (en) | 2018-12-13 |
| JP2018500932A (ja) | 2018-01-18 |
| EP3237636B1 (en) | 2020-10-21 |
| WO2016101959A1 (en) | 2016-06-30 |
| CA2970966A1 (en) | 2016-06-30 |
| US20210189468A1 (en) | 2021-06-24 |
| CN107109492A (zh) | 2017-08-29 |
| EP3237636A1 (en) | 2017-11-01 |
| CN107109492B (zh) | 2021-02-26 |
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