CN104125963B - 使用离子交换膜层析改进下游蛋白质纯化步骤的方法 - Google Patents

使用离子交换膜层析改进下游蛋白质纯化步骤的方法 Download PDF

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Publication number
CN104125963B
CN104125963B CN201280070369.9A CN201280070369A CN104125963B CN 104125963 B CN104125963 B CN 104125963B CN 201280070369 A CN201280070369 A CN 201280070369A CN 104125963 B CN104125963 B CN 104125963B
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antibody
membrane
polypeptide
antibodies
effluent
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Chinese (zh)
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CN104125963A (zh
Inventor
J·J·小比尔
A·M·布朗
C·J·多德
B·E·塞耶
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
CN201280070369.9A 2011-12-22 2012-12-18 使用离子交换膜层析改进下游蛋白质纯化步骤的方法 Active CN104125963B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810818291.3A CN108892706A (zh) 2011-12-22 2012-12-18 使用离子交换膜层析改进下游蛋白质纯化步骤的方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161579285P 2011-12-22 2011-12-22
US61/579,285 2011-12-22
PCT/US2012/070373 WO2013096322A1 (en) 2011-12-22 2012-12-18 Ion exchange membrane chromatography

Related Child Applications (1)

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CN201810818291.3A Division CN108892706A (zh) 2011-12-22 2012-12-18 使用离子交换膜层析改进下游蛋白质纯化步骤的方法

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CN104125963A CN104125963A (zh) 2014-10-29
CN104125963B true CN104125963B (zh) 2018-08-17

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CN201810818291.3A Pending CN108892706A (zh) 2011-12-22 2012-12-18 使用离子交换膜层析改进下游蛋白质纯化步骤的方法

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US (3) US10364268B2 (enExample)
EP (2) EP3492486A1 (enExample)
JP (5) JP6605202B2 (enExample)
KR (3) KR20210025692A (enExample)
CN (2) CN104125963B (enExample)
AR (1) AR089362A1 (enExample)
AU (3) AU2012355356B2 (enExample)
BR (1) BR112014015101A8 (enExample)
CA (1) CA2859376C (enExample)
ES (1) ES2697676T3 (enExample)
HR (1) HRP20181846T1 (enExample)
IL (2) IL267585B (enExample)
MX (2) MX360453B (enExample)
MY (1) MY172426A (enExample)
PL (1) PL2794635T3 (enExample)
RU (1) RU2648999C2 (enExample)
SG (1) SG11201403437TA (enExample)
SI (1) SI2794635T1 (enExample)
TR (1) TR201815709T4 (enExample)
WO (1) WO2013096322A1 (enExample)
ZA (2) ZA201404593B (enExample)

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CN104693303B (zh) * 2015-02-28 2018-03-13 苏州金盟生物技术有限公司 一种蛋白制品中的核酸去除方法
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KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법
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JP2023511946A (ja) * 2020-01-24 2023-03-23 アルカームス ファーマ アイルランド リミテッド 精製方法
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN116371011B (zh) * 2023-05-26 2023-08-18 湖南杰萃生物技术有限公司 一种从桑叶中提取黄酮和生物碱的方法
CN117866038B (zh) * 2024-03-11 2024-05-28 北京百力格生物科技有限公司 纯化含有宿主核酸的带亲和标签的酸性蛋白的方法

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IL267585B (en) 2022-09-01
CA2859376A1 (en) 2013-06-27
AU2012355356B2 (en) 2017-10-12
BR112014015101A2 (pt) 2017-06-13
TR201815709T4 (tr) 2018-11-21
NZ626949A (en) 2016-11-25
JP7402011B2 (ja) 2023-12-20
RU2648999C2 (ru) 2018-03-29
ES2697676T3 (es) 2019-01-25
IL267585A (en) 2019-08-29
JP2018109024A (ja) 2018-07-12
CN108892706A (zh) 2018-11-27
MX360453B (es) 2018-11-01
JP2020040952A (ja) 2020-03-19
JP7359807B2 (ja) 2023-10-11
KR20210025692A (ko) 2021-03-09
US20240247027A1 (en) 2024-07-25
AU2018200194A1 (en) 2018-02-01
SI2794635T1 (sl) 2018-12-31
CA2859376C (en) 2022-04-19
JP2022101557A (ja) 2022-07-06
WO2013096322A1 (en) 2013-06-27
AU2020201070A1 (en) 2020-03-05
IL233158B (en) 2019-07-31
EP2794635A1 (en) 2014-10-29
JP2021169454A (ja) 2021-10-28
KR102067075B1 (ko) 2020-01-17
KR20200008022A (ko) 2020-01-22
AU2018200194B2 (en) 2019-11-14
MX2014007622A (es) 2014-09-22
AR089362A1 (es) 2014-08-20
US20140348845A1 (en) 2014-11-27
AU2012355356A1 (en) 2014-07-10
ZA201404593B (en) 2022-12-21
EP3492486A1 (en) 2019-06-05
ZA201903994B (en) 2023-12-20
US11945837B2 (en) 2024-04-02
EP2794635B8 (en) 2018-11-14
WO2013096322A8 (en) 2014-02-27
IL233158A0 (en) 2014-07-31
JP2015503524A (ja) 2015-02-02
CN104125963A (zh) 2014-10-29
MY172426A (en) 2019-11-25
KR20140114834A (ko) 2014-09-29
US20200102346A1 (en) 2020-04-02
HK1203526A1 (en) 2015-10-30
SG11201403437TA (en) 2014-07-30
HRP20181846T1 (hr) 2018-12-28
EP2794635B1 (en) 2018-09-12
PL2794635T3 (pl) 2019-02-28
JP6605202B2 (ja) 2019-11-13
US10364268B2 (en) 2019-07-30
MX2018013356A (es) 2020-09-14
BR112014015101A8 (pt) 2021-06-08
WO2013096322A9 (en) 2013-08-22
RU2014130017A (ru) 2016-02-10

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