CN104066745B - 溶菌酶作为标签的用途 - Google Patents

溶菌酶作为标签的用途 Download PDF

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Publication number
CN104066745B
CN104066745B CN201380006389.4A CN201380006389A CN104066745B CN 104066745 B CN104066745 B CN 104066745B CN 201380006389 A CN201380006389 A CN 201380006389A CN 104066745 B CN104066745 B CN 104066745B
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CN
China
Prior art keywords
lysozyme
protein
polypeptide
albumen
fusion protein
Prior art date
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Expired - Fee Related
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CN201380006389.4A
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English (en)
Chinese (zh)
Other versions
CN104066745A (zh
Inventor
S·黑特尔
S·耶格
D·道贝特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morphosys GmbH
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Morphosys GmbH
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Publication of CN104066745A publication Critical patent/CN104066745A/zh
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380006389.4A 2012-01-23 2013-01-23 溶菌酶作为标签的用途 Expired - Fee Related CN104066745B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261589408P 2012-01-23 2012-01-23
US61/589,408 2012-01-23
EP12152095.1 2012-01-23
EP12152095 2012-01-23
PCT/EP2013/051181 WO2013110627A1 (en) 2012-01-23 2013-01-23 Use of lysozyme as a tag

Publications (2)

Publication Number Publication Date
CN104066745A CN104066745A (zh) 2014-09-24
CN104066745B true CN104066745B (zh) 2017-04-12

Family

ID=48872896

Family Applications (1)

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CN201380006389.4A Expired - Fee Related CN104066745B (zh) 2012-01-23 2013-01-23 溶菌酶作为标签的用途

Country Status (10)

Country Link
US (1) US9689014B2 (enExample)
EP (1) EP2807179B1 (enExample)
JP (1) JP6215231B2 (enExample)
KR (1) KR20140122729A (enExample)
CN (1) CN104066745B (enExample)
AU (1) AU2013211651C1 (enExample)
CA (1) CA2860916A1 (enExample)
ES (1) ES2582802T3 (enExample)
IN (1) IN2014DN06660A (enExample)
WO (1) WO2013110627A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105524942A (zh) * 2016-02-05 2016-04-27 广州赛莱拉干细胞科技股份有限公司 一种表达载体及胰岛素样生长因子结合蛋白3的表达方法
US10986850B2 (en) 2016-12-21 2021-04-27 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
EP3612212A4 (en) 2016-12-21 2020-10-21 Novozymes A/S POLYPEPTIDES HAVING LYSOZYME ACTIVITY, POLYNUCLEOTIDES CODING THEREOF AND USES AND COMPOSITIONS OF THEM
CN107794254A (zh) * 2017-09-30 2018-03-13 昆明理工大学 重组高温裂解酶的快速纯化方法
CN116568792A (zh) * 2020-12-16 2023-08-08 丹尼斯科美国公司 用于清洁的酶和酶组合物
WO2023168234A1 (en) * 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2025179145A1 (en) * 2024-02-23 2025-08-28 Dualitas Therapeutics, Inc. Bispecific screening compositions and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332323A1 (en) * 1988-03-10 1989-09-13 Chisso Corporation Carrier for affinity chromatography immobilized with antibodies and preparation thereof
WO2001000855A1 (en) * 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2459701A (en) 1945-07-16 1949-01-18 Harper Esther Pardee Mechanical instrument holder
US4355022A (en) 1981-07-01 1982-10-19 Interon, Inc. Method of dental treatment
EP0425016B1 (en) 1989-10-27 1995-12-20 The Procter & Gamble Company Antimicrobial method and formulation employing type II endoglycosidase and antimicrobial agent
DE59812393D1 (de) 1997-08-22 2005-01-20 Siemens Ag Verfahren zur klopfregelung einer brennkraftmaschine
JP2002527046A (ja) 1998-10-15 2002-08-27 デーエスエム・ナムローゼ・フェンノートシャップ 動物飼料中における抗微生物酵素
CA2472477C (en) 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification
DE10237317B4 (de) 2002-08-15 2010-04-08 3M Espe Ag Enzymhaltige Zusammensetzung, Verfahren zu deren Herstellung und deren Verwendung
CA2404356A1 (en) 2002-09-18 2004-03-18 Canadian Inovatech Inc. Gram-positive antibacterial composition and method for use
US7428273B2 (en) 2003-09-18 2008-09-23 Promptu Systems Corporation Method and apparatus for efficient preamble detection in digital data receivers
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
US8119600B2 (en) 2007-04-10 2012-02-21 Stefano Ferrari Compositions containing lysozyme and C-1/C-4 polysaccharides and use thereof in oral care, cosmetology and dermatology, contraception, urology and gynecology
WO2009051769A1 (en) * 2007-10-17 2009-04-23 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for crystallizing g protein-coupled receptors
CN101817883B (zh) * 2010-03-12 2012-06-13 广东药学院 一种家蝇天蚕素-人溶菌酶融合蛋白及其制备方法和应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332323A1 (en) * 1988-03-10 1989-09-13 Chisso Corporation Carrier for affinity chromatography immobilized with antibodies and preparation thereof
WO2001000855A1 (en) * 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
A Three-dimensional Model of an Anti-lysozyme Antibody;Sandra J.Smith-Gill et al.;《J.Mol.Biol.》;19870420;第194卷(第4期);713-724 *

Also Published As

Publication number Publication date
IN2014DN06660A (enExample) 2015-05-22
ES2582802T3 (es) 2016-09-15
JP6215231B2 (ja) 2017-10-18
AU2013211651B2 (en) 2017-03-02
KR20140122729A (ko) 2014-10-20
AU2013211651C1 (en) 2017-10-12
CA2860916A1 (en) 2013-08-01
EP2807179A1 (en) 2014-12-03
AU2013211651A1 (en) 2014-07-24
WO2013110627A1 (en) 2013-08-01
US20140349343A1 (en) 2014-11-27
US9689014B2 (en) 2017-06-27
EP2807179B1 (en) 2016-04-20
CN104066745A (zh) 2014-09-24
HK1202298A1 (en) 2015-09-25
JP2015504683A (ja) 2015-02-16

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