JP6215231B2 - リゾチームのタグとしての使用 - Google Patents

リゾチームのタグとしての使用 Download PDF

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Publication number
JP6215231B2
JP6215231B2 JP2014552662A JP2014552662A JP6215231B2 JP 6215231 B2 JP6215231 B2 JP 6215231B2 JP 2014552662 A JP2014552662 A JP 2014552662A JP 2014552662 A JP2014552662 A JP 2014552662A JP 6215231 B2 JP6215231 B2 JP 6215231B2
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JP
Japan
Prior art keywords
protein
lysozyme
polypeptide
amino acids
fusion protein
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Expired - Fee Related
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JP2014552662A
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English (en)
Japanese (ja)
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JP2015504683A (ja
Inventor
ヘルトル，シュテファン
イェーガー，セバスチャン
ダウベルト，ダニエラ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MorphoSys GmbH
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MorphoSys GmbH
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Publication date
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2014552662A 2012-01-23 2013-01-23 リゾチームのタグとしての使用 Expired - Fee Related JP6215231B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261589408P 2012-01-23 2012-01-23
EP12152095 2012-01-23
US61/589,408 2012-01-23
EP12152095.1 2012-01-23
PCT/EP2013/051181 WO2013110627A1 (en) 2012-01-23 2013-01-23 Use of lysozyme as a tag

Publications (2)

Publication Number Publication Date
JP2015504683A JP2015504683A (ja) 2015-02-16
JP6215231B2 true JP6215231B2 (ja) 2017-10-18

Family

ID=48872896

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014552662A Expired - Fee Related JP6215231B2 (ja) 2012-01-23 2013-01-23 リゾチームのタグとしての使用

Country Status (10)

Country Link
US (1) US9689014B2 (enExample)
EP (1) EP2807179B1 (enExample)
JP (1) JP6215231B2 (enExample)
KR (1) KR20140122729A (enExample)
CN (1) CN104066745B (enExample)
AU (1) AU2013211651C1 (enExample)
CA (1) CA2860916A1 (enExample)
ES (1) ES2582802T3 (enExample)
IN (1) IN2014DN06660A (enExample)
WO (1) WO2013110627A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105524942A (zh) * 2016-02-05 2016-04-27 广州赛莱拉干细胞科技股份有限公司 一种表达载体及胰岛素样生长因子结合蛋白3的表达方法
AU2017380488B2 (en) 2016-12-21 2023-09-14 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
AU2017384228B2 (en) 2016-12-21 2024-07-25 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN107794254A (zh) * 2017-09-30 2018-03-13 昆明理工大学 重组高温裂解酶的快速纯化方法
WO2022133068A1 (en) * 2020-12-16 2022-06-23 Danisco Us Inc Enzymes and enzyme compositions for cleaning
WO2023168234A1 (en) * 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
WO2025179145A1 (en) * 2024-02-23 2025-08-28 Dualitas Therapeutics, Inc. Bispecific screening compositions and methods

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2459701A (en) 1945-07-16 1949-01-18 Harper Esther Pardee Mechanical instrument holder
US4355022A (en) 1981-07-01 1982-10-19 Interon, Inc. Method of dental treatment
JP2761881B2 (ja) 1988-03-10 1998-06-04 チッソ株式会社 抗体を固定化したアフイニテイクロマトグラフイ用担体
DK0425016T3 (da) 1989-10-27 1996-05-06 Genencor Int Antimikrobiel fremgangsmåde og formulering med anvendelse af type II endoglycosidase og antimikrobielt middel
BR9811984A (pt) 1997-08-22 2000-09-05 Siemens Ag Processo para a regulagem de batida de uma máquina de combustão interna
BR9914555A (pt) 1998-10-15 2001-06-26 Dsm Nv Enzimas antimicrobiais em alimentação animal
WO2001000855A1 (en) 1999-06-23 2001-01-04 Ppl Therapeutics (Scotland) Ltd. Fusion proteins incorporating lysozyme
WO2003057174A2 (en) 2002-01-07 2003-07-17 Lifesensors, Inc. Methods and compositions for protein expression and purification
DE10237317B4 (de) 2002-08-15 2010-04-08 3M Espe Ag Enzymhaltige Zusammensetzung, Verfahren zu deren Herstellung und deren Verwendung
CA2404356A1 (en) 2002-09-18 2004-03-18 Canadian Inovatech Inc. Gram-positive antibacterial composition and method for use
US7428273B2 (en) 2003-09-18 2008-09-23 Promptu Systems Corporation Method and apparatus for efficient preamble detection in digital data receivers
AU2005215852B2 (en) 2004-02-25 2010-04-22 Novozymes A/S Fungal cell wall degrading enzyme
KR20100016416A (ko) 2007-04-10 2010-02-12 새인트 시메온 엘디에이 리소자임 및 ｃ-1/ｃ-4 다당류를 함유하는 조성물, 및 구강 관리, 미용학 및 피부과, 피임, 비뇨기과 및 부인과에서의 이의 용도
WO2009051769A1 (en) 2007-10-17 2009-04-23 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for crystallizing g protein-coupled receptors
CN101817883B (zh) * 2010-03-12 2012-06-13 广东药学院 一种家蝇天蚕素-人溶菌酶融合蛋白及其制备方法和应用

Also Published As

Publication number Publication date
US20140349343A1 (en) 2014-11-27
EP2807179A1 (en) 2014-12-03
JP2015504683A (ja) 2015-02-16
KR20140122729A (ko) 2014-10-20
CA2860916A1 (en) 2013-08-01
CN104066745A (zh) 2014-09-24
AU2013211651A1 (en) 2014-07-24
ES2582802T3 (es) 2016-09-15
AU2013211651B2 (en) 2017-03-02
US9689014B2 (en) 2017-06-27
HK1202298A1 (en) 2015-09-25
IN2014DN06660A (enExample) 2015-05-22
EP2807179B1 (en) 2016-04-20
AU2013211651C1 (en) 2017-10-12
CN104066745B (zh) 2017-04-12
WO2013110627A1 (en) 2013-08-01

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