CN103930541A - 用于增强甲羟戊酸、异戊二烯和类异戊二烯的产量的重组微生物 - Google Patents
用于增强甲羟戊酸、异戊二烯和类异戊二烯的产量的重组微生物 Download PDFInfo
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- CN103930541A CN103930541A CN201280031900.1A CN201280031900A CN103930541A CN 103930541 A CN103930541 A CN 103930541A CN 201280031900 A CN201280031900 A CN 201280031900A CN 103930541 A CN103930541 A CN 103930541A
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- C12Y101/01037—Malate dehydrogenase (1.1.1.37)
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- C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
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- C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
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- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
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- C12Y207/02001—Acetate kinase (2.7.2.1)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161481121P | 2011-04-29 | 2011-04-29 | |
| US61/481,121 | 2011-04-29 | ||
| PCT/US2012/035687 WO2012149491A2 (en) | 2011-04-29 | 2012-04-27 | Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103930541A true CN103930541A (zh) | 2014-07-16 |
Family
ID=46052905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280031900.1A Pending CN103930541A (zh) | 2011-04-29 | 2012-04-27 | 用于增强甲羟戊酸、异戊二烯和类异戊二烯的产量的重组微生物 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US9121038B2 (https=) |
| EP (1) | EP2702140B1 (https=) |
| JP (2) | JP6301827B2 (https=) |
| CN (1) | CN103930541A (https=) |
| AU (1) | AU2012249320A1 (https=) |
| BR (1) | BR112013027555A2 (https=) |
| CA (1) | CA2834633A1 (https=) |
| ES (1) | ES2699747T3 (https=) |
| SG (1) | SG194690A1 (https=) |
| WO (1) | WO2012149491A2 (https=) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105624180A (zh) * | 2016-02-06 | 2016-06-01 | 中国热带农业科学院橡胶研究所 | HbCS3基因在提高原核表达菌生长速率、研究橡胶树抗逆境胁迫和产胶能力中的应用 |
| CN105647953A (zh) * | 2016-03-18 | 2016-06-08 | 黑龙江大学 | 高产2,3-丁二醇的产酸克雷伯氏基因工程菌株的构建方法及其发酵方法 |
| CN105647954A (zh) * | 2016-03-18 | 2016-06-08 | 黑龙江大学 | 高产2,3-丁二醇的产酸克雷伯氏基因工程菌株的构建方法及其发酵方法 |
| CN106148262A (zh) * | 2016-07-01 | 2016-11-23 | 江南大学 | 提高乙酰氨基葡萄糖产量的重组枯草芽孢杆菌及其构建方法 |
| CN106715687A (zh) * | 2015-03-18 | 2017-05-24 | Cj第制糖株式会社 | 丙酮酸脱氢酶突变体、包含突变体的微生物和通过使用微生物生产l‑氨基酸的方法 |
| CN107937431A (zh) * | 2017-10-28 | 2018-04-20 | 国际竹藤中心 | 一种提高酿酒酵母合成芳樟醇能力的方法 |
| CN108179114A (zh) * | 2017-11-27 | 2018-06-19 | 南京晓庄学院 | 产抗厌氧菌化合物的菌株和发酵方法、抗厌氧菌化合物提取及制备方法和使用方法 |
| CN109722403A (zh) * | 2017-10-30 | 2019-05-07 | 中国石油化工股份有限公司 | 一种利用纤维素制备金合欢烯的工程菌株及方法 |
| CN110964680A (zh) * | 2018-09-29 | 2020-04-07 | 中国石油化工股份有限公司 | 利用纤维素制备金合欢烯的工程菌株及方法 |
| CN110964679A (zh) * | 2018-09-29 | 2020-04-07 | 中国石油化工股份有限公司 | 一种利用纤维素制备金合欢烯的工程菌株及方法 |
| CN115704038A (zh) * | 2021-08-10 | 2023-02-17 | 西北农林科技大学 | 一种基因、重组载体、工程菌及其应用 |
| CN115948444A (zh) * | 2022-10-21 | 2023-04-11 | 江南大学 | 一种利用硝酸盐和全局厌氧调节因子Fnr提高地衣芽孢杆菌厌氧活性的方法 |
| CN116925991A (zh) * | 2023-07-28 | 2023-10-24 | 天津大学 | 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用 |
| CN119286752A (zh) * | 2024-12-10 | 2025-01-10 | 杭州合碳创物科技有限公司 | 一种生产甲瓦龙酸的重组大肠杆菌及其应用 |
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| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
| BR112013027555A2 (pt) * | 2011-04-29 | 2021-08-10 | Danisco Us Inc. | células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides |
| US20140128645A1 (en) * | 2011-12-23 | 2014-05-08 | The Goodyear Tire & Rubber Company | Enhanced production of isoprene using marine bacterial cells |
| WO2013181647A2 (en) * | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
| US20140024872A1 (en) * | 2012-07-13 | 2014-01-23 | Calysta Energy, Llc | Biorefinery system, methods and compositions thereof |
| AU2013299608B2 (en) | 2012-08-07 | 2019-02-21 | Amyris, Inc. | Methods for stabilizing production of acetyl-coenzyme a derived compounds |
| MX364748B (es) * | 2013-03-15 | 2019-05-06 | Amyris Inc | Uso de fosfoquetolasa y fosfotransacetilasa para la produccion de compuestos derivados de acetil-coenzima a. |
| SG11201508186XA (en) * | 2013-04-10 | 2015-11-27 | Danisco Us Inc | Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
| JP6139244B2 (ja) * | 2013-04-23 | 2017-05-31 | 住友ゴム工業株式会社 | ポリイソプレノイド増産方法、およびそのための増産剤、かかる方法で製造されたポリイソプレノイド |
| RU2013146377A (ru) | 2013-10-17 | 2015-04-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ ИЗОПРЕНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ РОДА Bacillus |
| US20160237398A1 (en) * | 2013-10-18 | 2016-08-18 | University Of Washington Through Its Center For Commercialization | Methods of microbial production of excreted products from methane and related bacterial strains |
| WO2015058188A1 (en) * | 2013-10-18 | 2015-04-23 | University Of Washington Through Its Center For Commercialization | Modified group i methanotrophic bacteria and uses thereof |
| US20160376600A1 (en) | 2013-11-25 | 2016-12-29 | Genomatica, Inc. | Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol |
| EP4296364B1 (en) | 2013-12-03 | 2025-12-03 | Genomatica, Inc. | Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis |
| CN104164457B (zh) * | 2014-07-15 | 2016-05-04 | 青岛农业大学 | 一种利用新mva途径合成异戊二烯的方法及其相应重组细胞和应用 |
| JP6470532B2 (ja) * | 2014-09-17 | 2019-02-13 | 積水化学工業株式会社 | 組換え細胞、並びに、有機化合物の生産方法 |
| CN104593405A (zh) * | 2014-11-24 | 2015-05-06 | 安徽大学 | 一种高产丙氨酸的枯草杆菌工程菌的构建方法 |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| CN109234216B (zh) * | 2017-07-10 | 2022-05-20 | 上海医药工业研究院 | 一种生产鲨烯的基因工程菌及其方法 |
| WO2019113387A1 (en) | 2017-12-07 | 2019-06-13 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| JP2021506316A (ja) | 2017-12-21 | 2021-02-22 | ザイマージェン インコーポレイテッド | ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物 |
| PL3762499T3 (pl) * | 2018-03-06 | 2024-06-24 | Danisco Us Inc | Redukcja wytwarzania octanu przez drożdże wykazujące nadmierną ekspresję pab1 |
| WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
| US11845940B2 (en) * | 2020-02-27 | 2023-12-19 | The Regents Of The University Of California | Genetically modified fungal cells and methods useful for producing prespatane |
| EP4179067A1 (en) * | 2020-07-08 | 2023-05-17 | The Regents of University of California | Modified yeast host cells useful for producing isoprenol |
| WO2022032084A1 (en) * | 2020-08-06 | 2022-02-10 | Synlogic Operating Company, Inc. | Recombinant bacteria for production of d-lactate and/or l-lactate and uses thereof |
| JP7827466B2 (ja) * | 2022-01-19 | 2026-03-10 | 旭化成株式会社 | 改変微生物及び化合物の製造方法 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4168870B2 (ja) * | 2003-08-14 | 2008-10-22 | トヨタ自動車株式会社 | プレニルアルコールの製造方法 |
| US20100196977A1 (en) * | 2008-12-30 | 2010-08-05 | Chotani Gopal K | Methods of producing isoprene and a co-product |
| WO2010148256A1 (en) * | 2009-06-17 | 2010-12-23 | Danisco Us Inc. | Fuel compositions comprising isoprene derivatives |
| US20110053216A1 (en) * | 2006-10-20 | 2011-03-03 | Vermaas Willem F J | Modified Cyanobacteria |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19629568C1 (de) | 1996-07-15 | 1998-01-08 | Fraunhofer Ges Forschung | Verfahren zur Herstellung von Isopren |
| WO2000078935A1 (en) | 1999-06-22 | 2000-12-28 | Smithkline Beecham Corporation | Mevalonate pathway genes |
| US7172886B2 (en) | 2001-12-06 | 2007-02-06 | The Regents Of The University Of California | Biosynthesis of isopentenyl pyrophosphate |
| JPWO2003095651A1 (ja) | 2002-05-10 | 2005-09-15 | 協和醗酵工業株式会社 | メバロン酸の製造方法 |
| KR101148255B1 (ko) | 2002-10-04 | 2012-08-08 | 다니스코 유에스 인크. | 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법 |
| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| WO2007140339A2 (en) * | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Production of isoprenoids |
| US7947478B2 (en) | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| JP4986547B2 (ja) | 2006-09-04 | 2012-07-25 | 株式会社Adeka | 新規なアセトアセチルCoA合成酵素、それをコードするDNA配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法 |
| US7947483B2 (en) * | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| CN105368764B (zh) | 2007-12-13 | 2019-03-12 | 丹尼斯科美国公司 | 用于生产异戊二烯的组合物和方法 |
| US20110045563A1 (en) | 2008-02-06 | 2011-02-24 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| CA2728285A1 (en) * | 2008-03-03 | 2009-09-11 | Joule Unlimited, Inc. | Engineered co2 fixing microorganisms producing carbon-based products of interest |
| WO2009132220A2 (en) | 2008-04-23 | 2009-10-29 | Danisco Us Inc. | Isoprene synthase variants for improved microbial production of isoprene |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| CA2725175A1 (en) * | 2008-05-23 | 2009-11-26 | Cibus Oils, Llc | Production of squalene using yeast |
| MX318543B (es) | 2008-07-02 | 2014-03-18 | Danisco Us Inc | Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros. |
| HU3516U (en) | 2008-07-31 | 2008-11-28 | Zoltan Kuthi | Anti-theft protection of draining for fuel tanks |
| EP2340312A2 (en) | 2008-09-15 | 2011-07-06 | Danisco US Inc. | Conversion of prenyl derivatives to isoprene |
| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| WO2010031062A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using the archaeal lower mevalonate pathway |
| CA2737082A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Increased isoprene production using mevalonate kinase and isoprene synthase |
| EP2344629B1 (en) | 2008-09-15 | 2018-01-24 | Danisco US Inc. | Systems using cell culture for production of isoprene |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| AU2010238770A1 (en) | 2009-04-23 | 2011-11-03 | Danisco Us Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP4760951B2 (ja) | 2009-05-08 | 2011-08-31 | トヨタ自動車株式会社 | ブタノール生産能を有する組換え微生物及びブタノールの製造方法 |
| TW201412988A (zh) * | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用dxp及mva途徑之改良之異戊二烯製造 |
| US20120252054A1 (en) | 2009-09-15 | 2012-10-04 | Sapphire Energy Inc | System for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| HUE034245T2 (en) * | 2009-11-23 | 2018-02-28 | Nucelis Llc | Methods and preparations for the preparation of squalene using yeast |
| US8569562B2 (en) | 2009-12-18 | 2013-10-29 | Danisco Us Inc. | Purification of isoprene from renewable resources |
| SG196782A1 (en) | 2009-12-23 | 2014-02-13 | Danisco Us Inc | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl) |
| CA2807558A1 (en) * | 2010-08-06 | 2012-02-09 | Danisco Us Inc. | Production of isoprene under neutral ph conditions |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| BR112013018316A2 (pt) * | 2010-12-22 | 2018-09-11 | Danisco Us Inc | composições e métodos para produção aprimorada de isopreno usando dois tipos de enzimas ispg |
| BR112013027555A2 (pt) | 2011-04-29 | 2021-08-10 | Danisco Us Inc. | células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides |
| SG194691A1 (en) * | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
-
2012
- 2012-04-27 BR BR112013027555-3A patent/BR112013027555A2/pt not_active Application Discontinuation
- 2012-04-27 EP EP12720353.7A patent/EP2702140B1/en active Active
- 2012-04-27 CA CA2834633A patent/CA2834633A1/en not_active Abandoned
- 2012-04-27 JP JP2014508164A patent/JP6301827B2/ja active Active
- 2012-04-27 ES ES12720353T patent/ES2699747T3/es active Active
- 2012-04-27 US US13/459,067 patent/US9121038B2/en active Active
- 2012-04-27 SG SG2013080304A patent/SG194690A1/en unknown
- 2012-04-27 CN CN201280031900.1A patent/CN103930541A/zh active Pending
- 2012-04-27 AU AU2012249320A patent/AU2012249320A1/en not_active Abandoned
- 2012-04-27 WO PCT/US2012/035687 patent/WO2012149491A2/en not_active Ceased
-
2015
- 2015-07-22 US US14/806,343 patent/US10138498B2/en active Active
-
2017
- 2017-04-20 JP JP2017083583A patent/JP2017148063A/ja active Pending
-
2018
- 2018-11-26 US US16/200,485 patent/US10975394B2/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4168870B2 (ja) * | 2003-08-14 | 2008-10-22 | トヨタ自動車株式会社 | プレニルアルコールの製造方法 |
| US20110053216A1 (en) * | 2006-10-20 | 2011-03-03 | Vermaas Willem F J | Modified Cyanobacteria |
| US20100196977A1 (en) * | 2008-12-30 | 2010-08-05 | Chotani Gopal K | Methods of producing isoprene and a co-product |
| WO2010148256A1 (en) * | 2009-06-17 | 2010-12-23 | Danisco Us Inc. | Fuel compositions comprising isoprene derivatives |
Non-Patent Citations (2)
| Title |
|---|
| ANTHONY P.BURGARD等: "OptKnock:A Bilevel Programming Framework for identifying Gene Knockout Strategies for Microbial Strain Optimization.", 《BIOTECHNOLOGY AND BIOENGINEERING》, vol. 85, no. 7, 31 December 2003 (2003-12-31), pages 1 - 11 * |
| 张园园: "大肠杆菌甲羟戊酸途径的构建与调控", 《山东大学硕士学位论文》, 1 February 2010 (2010-02-01) * |
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| US20160032323A1 (en) | 2016-02-04 |
| BR112013027555A2 (pt) | 2021-08-10 |
| ES2699747T3 (es) | 2019-02-12 |
| US9121038B2 (en) | 2015-09-01 |
| WO2012149491A2 (en) | 2012-11-01 |
| US10138498B2 (en) | 2018-11-27 |
| WO2012149491A3 (en) | 2012-12-27 |
| JP2017148063A (ja) | 2017-08-31 |
| US10975394B2 (en) | 2021-04-13 |
| JP2014519812A (ja) | 2014-08-21 |
| SG194690A1 (en) | 2013-12-30 |
| CA2834633A1 (en) | 2012-11-01 |
| EP2702140A2 (en) | 2014-03-05 |
| US20120276587A1 (en) | 2012-11-01 |
| AU2012249320A2 (en) | 2013-11-28 |
| JP6301827B2 (ja) | 2018-03-28 |
| EP2702140B1 (en) | 2018-10-31 |
| AU2012249320A1 (en) | 2013-11-14 |
| US20190153479A1 (en) | 2019-05-23 |
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