ES2699747T3 - Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides - Google Patents

Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides Download PDF

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Publication number
ES2699747T3
ES2699747T3 ES12720353T ES12720353T ES2699747T3 ES 2699747 T3 ES2699747 T3 ES 2699747T3 ES 12720353 T ES12720353 T ES 12720353T ES 12720353 T ES12720353 T ES 12720353T ES 2699747 T3 ES2699747 T3 ES 2699747T3
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gene
endogenous
activity
mevalonate
cells
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Spanish (es)
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Zachary Beck
Michael Miller
Caroline Peres
Yuliya Primak
Jeff Pucci
Derek Wells
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Goodyear Tire and Rubber Co
Danisco US Inc
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Goodyear Tire and Rubber Co
Danisco US Inc
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12N9/88—Lyases (4.)
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01034—Hydroxymethylglutaryl-CoA reductase (NADPH) (1.1.1.34)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01051—Pyruvate dehydrogenase (NADP+) (1.2.1.51)
    • C—CHEMISTRY; METALLURGY
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    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04001—Pyruvate dehydrogenase (acetyl-transferring) (1.2.4.1)
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01016—Acetyl-CoA C-acyltransferase (2.3.1.16)
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03001—Citrate (Si)-synthase (2.3.3.1)
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    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/0301—Hydroxymethylglutaryl-CoA synthase (2.3.3.10)
    • C—CHEMISTRY; METALLURGY
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02001—Acetate kinase (2.7.2.1)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01031—6-Phosphogluconolactonase (3.1.1.31)
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES12720353T 2011-04-29 2012-04-27 Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides Active ES2699747T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161481121P 2011-04-29 2011-04-29
PCT/US2012/035687 WO2012149491A2 (en) 2011-04-29 2012-04-27 Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids

Publications (1)

Publication Number Publication Date
ES2699747T3 true ES2699747T3 (es) 2019-02-12

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ES12720353T Active ES2699747T3 (es) 2011-04-29 2012-04-27 Microorganismos recombinantes para la producción potenciada de mevalonato, isopreno e isoprenoides

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Country Link
US (3) US9121038B2 (https=)
EP (1) EP2702140B1 (https=)
JP (2) JP6301827B2 (https=)
CN (1) CN103930541A (https=)
AU (1) AU2012249320A1 (https=)
BR (1) BR112013027555A2 (https=)
CA (1) CA2834633A1 (https=)
ES (1) ES2699747T3 (https=)
SG (1) SG194690A1 (https=)
WO (1) WO2012149491A2 (https=)

Families Citing this family (43)

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BR112013027555A2 (pt) * 2011-04-29 2021-08-10 Danisco Us Inc. células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides
US20140128645A1 (en) * 2011-12-23 2014-05-08 The Goodyear Tire & Rubber Company Enhanced production of isoprene using marine bacterial cells
WO2013181647A2 (en) * 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
US20140024872A1 (en) * 2012-07-13 2014-01-23 Calysta Energy, Llc Biorefinery system, methods and compositions thereof
AU2013299608B2 (en) 2012-08-07 2019-02-21 Amyris, Inc. Methods for stabilizing production of acetyl-coenzyme a derived compounds
MX364748B (es) * 2013-03-15 2019-05-06 Amyris Inc Uso de fosfoquetolasa y fosfotransacetilasa para la produccion de compuestos derivados de acetil-coenzima a.
SG11201508186XA (en) * 2013-04-10 2015-11-27 Danisco Us Inc Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids
JP6139244B2 (ja) * 2013-04-23 2017-05-31 住友ゴム工業株式会社 ポリイソプレノイド増産方法、およびそのための増産剤、かかる方法で製造されたポリイソプレノイド
RU2013146377A (ru) 2013-10-17 2015-04-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") СПОСОБ ПОЛУЧЕНИЯ ИЗОПРЕНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ РОДА Bacillus
US20160237398A1 (en) * 2013-10-18 2016-08-18 University Of Washington Through Its Center For Commercialization Methods of microbial production of excreted products from methane and related bacterial strains
WO2015058188A1 (en) * 2013-10-18 2015-04-23 University Of Washington Through Its Center For Commercialization Modified group i methanotrophic bacteria and uses thereof
US20160376600A1 (en) 2013-11-25 2016-12-29 Genomatica, Inc. Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol
EP4296364B1 (en) 2013-12-03 2025-12-03 Genomatica, Inc. Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis
CN104164457B (zh) * 2014-07-15 2016-05-04 青岛农业大学 一种利用新mva途径合成异戊二烯的方法及其相应重组细胞和应用
JP6470532B2 (ja) * 2014-09-17 2019-02-13 積水化学工業株式会社 組換え細胞、並びに、有機化合物の生産方法
CN104593405A (zh) * 2014-11-24 2015-05-06 安徽大学 一种高产丙氨酸的枯草杆菌工程菌的构建方法
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US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN105624180B (zh) * 2016-02-06 2018-12-28 中国热带农业科学院橡胶研究所 HbCS3基因在提高原核表达菌生长速率、研究橡胶树抗逆境胁迫和产胶能力中的应用
CN105647953A (zh) * 2016-03-18 2016-06-08 黑龙江大学 高产2,3-丁二醇的产酸克雷伯氏基因工程菌株的构建方法及其发酵方法
CN105647954A (zh) * 2016-03-18 2016-06-08 黑龙江大学 高产2,3-丁二醇的产酸克雷伯氏基因工程菌株的构建方法及其发酵方法
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US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
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