SG194690A1 - Recombinant microorganisms for enhanced production of mevalonate,isoprene, and isoprenoids - Google Patents

Recombinant microorganisms for enhanced production of mevalonate,isoprene, and isoprenoids Download PDF

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Publication number
SG194690A1
SG194690A1 SG2013080304A SG2013080304A SG194690A1 SG 194690 A1 SG194690 A1 SG 194690A1 SG 2013080304 A SG2013080304 A SG 2013080304A SG 2013080304 A SG2013080304 A SG 2013080304A SG 194690 A1 SG194690 A1 SG 194690A1
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Singapore
Prior art keywords
mevalonate
isoprenoids
isoprene
production
enterococcus
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SG2013080304A
Inventor
Zachary Q Beck
Michael C Miller
Caroline M Peres
Yuliya A Primak
Jeff P Pucci
Derek H Wells
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Danisco Us Inc
Goodyear Tire & Rubber
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Application filed by Danisco Us Inc, Goodyear Tire & Rubber filed Critical Danisco Us Inc
Publication of SG194690A1 publication Critical patent/SG194690A1/en

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Abstract

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).
SG2013080304A 2011-04-29 2012-04-27 Recombinant microorganisms for enhanced production of mevalonate,isoprene, and isoprenoids SG194690A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161481121P 2011-04-29 2011-04-29
PCT/US2012/035687 WO2012149491A2 (en) 2011-04-29 2012-04-27 Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids

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SG194690A1 true SG194690A1 (en) 2013-12-30

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US (3) US9121038B2 (en)
EP (1) EP2702140B1 (en)
JP (2) JP6301827B2 (en)
CN (1) CN103930541A (en)
AU (1) AU2012249320A1 (en)
BR (1) BR112013027555A2 (en)
CA (1) CA2834633A1 (en)
ES (1) ES2699747T3 (en)
SG (1) SG194690A1 (en)
WO (1) WO2012149491A2 (en)

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