WO2012177599A3 - Microorganisms for producing n-propanol 1, 3-propanediol, 1,2-propanediol or glycerol and methods related thereto - Google Patents

Microorganisms for producing n-propanol 1, 3-propanediol, 1,2-propanediol or glycerol and methods related thereto Download PDF

Info

Publication number
WO2012177599A3
WO2012177599A3 PCT/US2012/043086 US2012043086W WO2012177599A3 WO 2012177599 A3 WO2012177599 A3 WO 2012177599A3 US 2012043086 W US2012043086 W US 2012043086W WO 2012177599 A3 WO2012177599 A3 WO 2012177599A3
Authority
WO
WIPO (PCT)
Prior art keywords
propanediol
propanol
microorganisms
glycerol
producing
Prior art date
Application number
PCT/US2012/043086
Other languages
French (fr)
Other versions
WO2012177599A2 (en
Inventor
Anthony P. Burgard
Robin E. Osterhout
Jun Sun
Priti Pharkya
Original Assignee
Genomatica, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica, Inc. filed Critical Genomatica, Inc.
Publication of WO2012177599A2 publication Critical patent/WO2012177599A2/en
Publication of WO2012177599A3 publication Critical patent/WO2012177599A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein is a non-naturally occurring microbial organism having a reductive TCA or Wood-Ljungdahl pathway and comprising at least one exogenous nucleic acid encoding said pathway enzymes expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. Also provided herein is a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, and can include culturing this organism for a sufficient period of time to produce a product.
PCT/US2012/043086 2011-06-22 2012-06-19 Microorganisms for producing n-propanol 1, 3-propanediol, 1,2-propanediol or glycerol and methods related thereto WO2012177599A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161500122P 2011-06-22 2011-06-22
US61/500,122 2011-06-22
US201161502292P 2011-06-28 2011-06-28
US61/502,292 2011-06-28

Publications (2)

Publication Number Publication Date
WO2012177599A2 WO2012177599A2 (en) 2012-12-27
WO2012177599A3 true WO2012177599A3 (en) 2013-02-21

Family

ID=47423163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/043086 WO2012177599A2 (en) 2011-06-22 2012-06-19 Microorganisms for producing n-propanol 1, 3-propanediol, 1,2-propanediol or glycerol and methods related thereto

Country Status (1)

Country Link
WO (1) WO2012177599A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140134690A1 (en) * 2012-11-06 2014-05-15 University Of Georgia Research Foundation, Inc. Microbes and methods for producing 1-propanol
US20140273123A1 (en) * 2013-03-14 2014-09-18 Richard Tobey Method for production of n-propanol and other C3-carbon containing products from syngas by symbiotic arrangement of C1-fixing and C3-producing anaerobic microorganism cultures
BR112015030208A2 (en) * 2013-06-05 2017-08-22 Lanzatech New Zealand Ltd METHODS FOR PRODUCING A FERMENTATION PRODUCT AND A MICROORGANISM, AND, RECOMBINANT MICROORGANISM
JP2016165225A (en) 2013-07-09 2016-09-15 味の素株式会社 Method for producing useful substance
US20150064759A1 (en) * 2013-09-05 2015-03-05 Braskem S.A. Modified microorganism and methods of using same for producing 2-propanol and1-propanol and/or 1,2-propanediol
PL3077501T3 (en) 2013-12-03 2022-01-31 Genomatica, Inc. Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis
CN106062178B (en) 2013-12-27 2021-07-27 基因组股份公司 Methods and organisms with increased carbon flux efficiency
DK3194604T3 (en) 2014-09-18 2020-05-25 Genomatica Inc NON-NATURAL MICROBIAL ORGANISMS WITH IMPROVED ENERGY EFFICIENCY
CN107404882A (en) 2015-02-27 2017-11-28 白狗实验室有限公司 For producing acetone, isopropanol, butyric acid, other biological product and its mixotrophism fermentation process of mixture
CN115322261A (en) 2015-10-30 2022-11-11 基因组股份公司 Methanol dehydrogenase fusion protein
US20210079334A1 (en) 2018-01-30 2021-03-18 Genomatica, Inc. Fermentation systems and methods with substantially uniform volumetric uptake rate of a reactive gaseous component
KR102201720B1 (en) * 2018-05-24 2021-01-12 한화솔루션 주식회사 Recombinant Corynebacterium Producing 1,3-PDO and Inhibited 3-HP Production and Method for Preparing 1,3-PDO Using Thereof
CA3103377A1 (en) 2018-06-26 2020-01-02 Genomatica, Inc. Engineered microorganisms with g3p---> 3pg enzyme and/or fructose-1,6-bisphosphatase including those having synthetic or enhanced methylotrophy
CN112795586B (en) * 2021-01-25 2023-07-04 南京林业大学 Carboxylic acid reductase recombinant plasmid, construction method and application thereof
CN115537406B (en) * 2021-06-30 2024-04-12 尚科生物医药（上海）有限公司 Ketoreductase and application thereof in preparation of (S) -1- (4-pyridyl) -1, 3-propanediol
CN116064546B (en) * 2022-11-21 2024-08-27 中国科学院天津工业生物技术研究所 Promoter for regulating and controlling butyric acid production and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090148914A1 (en) * 2002-11-06 2009-06-11 Causey Thomas B Materials and methods for the efficient production of acetate and other products
US20090191599A1 (en) * 2007-09-10 2009-07-30 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
US20100323418A1 (en) * 2009-04-30 2010-12-23 Genomatica, Inc. ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL, n-BUTANOL, AND ISOBUTANOL
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090148914A1 (en) * 2002-11-06 2009-06-11 Causey Thomas B Materials and methods for the efficient production of acetate and other products
US20090191599A1 (en) * 2007-09-10 2009-07-30 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
US20100323418A1 (en) * 2009-04-30 2010-12-23 Genomatica, Inc. ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL, n-BUTANOL, AND ISOBUTANOL
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids

Also Published As

Publication number Publication date
WO2012177599A2 (en) 2012-12-27

Similar Documents

Publication Publication Date Title
WO2012177599A3 (en) Microorganisms for producing n-propanol 1, 3-propanediol, 1,2-propanediol or glycerol and methods related thereto
WO2012177619A3 (en) Microorganisms for producing 1,3-butanediol and methods related thereto
MX2014003212A (en) Microorganisms and methods for producing alkenes.
WO2009135074A3 (en) Microorganisms for the production of methacrylic acid
MX348612B (en) Microorganisms and methods for producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol and related alcohols.
WO2012094425A3 (en) Biological methods for preparing adipic acid
WO2012135789A3 (en) Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto
WO2013006733A3 (en) Biological methods for preparing a fatty dicarboxylic acid
MY173771A (en) A method for producing a target substance by fermentation
SG179048A1 (en) Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
MX2009010224A (en) Microorganisms and methods for production of 1,2-propanediol and acetol.
MX2013008988A (en) Microorganisms and methods for the biosynthesis of butadiene.
IN2012DN00462A (en)
MX2012006602A (en) Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol.
WO2007116955A3 (en) Method for producing l-amino acid
WO2009028338A1 (en) Novel oxidase gene and method of producing 3-indole-pyruvic acid using the gene
WO2012177983A3 (en) Microorganisms for producing ethylene glycol and methods related thereto
MY171760A (en) Microorganisms and methods for the production of caprolactone
WO2013178936A3 (en) Method for continuously enriching an oil produced by microalgae with ethyl esters of dha
MY160740A (en) Method of producing lauric acid-containing oil or fat
MY163228A (en) Process for Producing 3-Hydroxybutyric Acid or Salt Thereof
WO2014052920A3 (en) Methods of producing acetoin and 2,3-butanediol using photosynthetic microorganisms
WO2013011292A3 (en) Microorganism for converting carbon dioxide to aliphatic carboxylic acids via formic acid intermediate
BR112014001174A2 (en) methods for increasing product yields
MY163223A (en) Method for producing hydrocarbons

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12802593

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12802593

Country of ref document: EP

Kind code of ref document: A2