CN103827304B - 用于异丁醇生产的宿主细胞和方法 - Google Patents
用于异丁醇生产的宿主细胞和方法 Download PDFInfo
- Publication number
- CN103827304B CN103827304B CN201280024903.2A CN201280024903A CN103827304B CN 103827304 B CN103827304 B CN 103827304B CN 201280024903 A CN201280024903 A CN 201280024903A CN 103827304 B CN103827304 B CN 103827304B
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- Prior art keywords
- host cell
- activity
- polypeptide
- seq
- isobutanol
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/52—Propionic acid; Butyric acids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (25)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161467261P | 2011-03-24 | 2011-03-24 | |
| US201161467271P | 2011-03-24 | 2011-03-24 | |
| US201161467249P | 2011-03-24 | 2011-03-24 | |
| US61/467,261 | 2011-03-24 | ||
| US61/467261 | 2011-03-24 | ||
| US61/467271 | 2011-03-24 | ||
| US61/467,249 | 2011-03-24 | ||
| US61/467,271 | 2011-03-24 | ||
| US61/467249 | 2011-03-24 | ||
| US201161472474P | 2011-04-06 | 2011-04-06 | |
| US201161472487P | 2011-04-06 | 2011-04-06 | |
| US201161472497P | 2011-04-06 | 2011-04-06 | |
| US201161472484P | 2011-04-06 | 2011-04-06 | |
| US61/472484 | 2011-04-06 | ||
| US61/472,497 | 2011-04-06 | ||
| US61/472487 | 2011-04-06 | ||
| US61/472,474 | 2011-04-06 | ||
| US61/472497 | 2011-04-06 | ||
| US61/472,487 | 2011-04-06 | ||
| US61/472,484 | 2011-04-06 | ||
| US61/472474 | 2011-04-06 | ||
| US201161570513P | 2011-12-14 | 2011-12-14 | |
| US61/570,513 | 2011-12-14 | ||
| US61/570513 | 2011-12-14 | ||
| PCT/US2012/030479 WO2012129555A2 (en) | 2011-03-24 | 2012-03-23 | Host cells and methods for production of isobutanol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103827304A CN103827304A (zh) | 2014-05-28 |
| CN103827304B true CN103827304B (zh) | 2018-04-17 |
Family
ID=46880082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280024903.2A Expired - Fee Related CN103827304B (zh) | 2011-03-24 | 2012-03-23 | 用于异丁醇生产的宿主细胞和方法 |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US9790521B2 (enExample) |
| EP (1) | EP2689014B1 (enExample) |
| JP (1) | JP6099623B2 (enExample) |
| KR (1) | KR20140092759A (enExample) |
| CN (1) | CN103827304B (enExample) |
| AR (1) | AR088778A1 (enExample) |
| AU (2) | AU2012230737B2 (enExample) |
| BR (1) | BR112013024328A2 (enExample) |
| CA (1) | CA2831130C (enExample) |
| HU (1) | HUE045191T2 (enExample) |
| MX (1) | MX347976B (enExample) |
| WO (1) | WO2012129555A2 (enExample) |
| ZA (1) | ZA201306708B (enExample) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| JP5582576B2 (ja) | 2007-04-18 | 2014-09-03 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | 活性の高いケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生成 |
| US8188250B2 (en) * | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
| KR20110063576A (ko) | 2008-09-29 | 2011-06-10 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 효모에서 증가된 이종성 Fe-S 효소 활성 |
| CA2784903A1 (en) | 2009-12-29 | 2011-07-28 | Butamax(Tm) Advanced Biofuels Llc | Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols |
| SG183294A1 (en) | 2010-02-12 | 2012-09-27 | Gevo Inc | Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids |
| MX348605B (es) | 2010-02-17 | 2017-06-21 | Butamax(Tm) Advanced Biofuels Llc | Mejoramiento de la actividad de proteinas que requieren centros fe-s. |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| NZ722791A (en) | 2010-09-07 | 2018-12-21 | Butamax Tm Advanced Biofuels | Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion |
| KR20140092759A (ko) | 2011-03-24 | 2014-07-24 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 숙주 세포 및 아이소부탄올의 제조 방법 |
| US20120258873A1 (en) * | 2011-04-06 | 2012-10-11 | Butamax(Tm) Advanced Biofuels Llc | Reduction of 2,3-dihydroxy-2-methyl butyrate (dhmb) in butanol production |
| MX2014000966A (es) | 2011-07-28 | 2014-07-09 | Butamax Advanced Biofuels Llc | Enzimas cetoisovalerato descarboxilasa y metodos de uso de las mismas. |
| JP2015503351A (ja) | 2011-12-30 | 2015-02-02 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | ブタノール生産のための遺伝的スイッチ |
| CA2861613A1 (en) | 2011-12-30 | 2013-07-04 | Butamax Advanced Biofuels Llc | Fermentative production of alcohols |
| KR20140146616A (ko) | 2012-03-23 | 2014-12-26 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 부타놀로겐용 배지의 아세테이트 보충물 |
| NZ701099A (en) | 2012-05-11 | 2017-04-28 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase enzymes and methods of use |
| BR112015003701A2 (pt) | 2012-08-22 | 2017-12-12 | Butamax Advanced Biofuels Llc | células hospedeiras recombinantes, método para aprimoramento, processo para a produção de um álcool, polinucleotídeo isolado, cassete de expressão e composição |
| US9840724B2 (en) | 2012-09-21 | 2017-12-12 | Butamax Advanced Biofuels Llc | Production of renewable hydrocarbon compositions |
| CN105102616A (zh) * | 2012-09-26 | 2015-11-25 | 布特马斯先进生物燃料有限责任公司 | 具有酮醇酸还原异构酶活性的多肽 |
| AU2013323396B2 (en) | 2012-09-28 | 2017-04-20 | Butamax Advanced Biofuels Llc | Production of fermentation products |
| US9273330B2 (en) | 2012-10-03 | 2016-03-01 | Butamax Advanced Biofuels Llc | Butanol tolerance in microorganisms |
| WO2014081848A1 (en) | 2012-11-20 | 2014-05-30 | Butamax Advanced Biofuels Llc | Butanol purification |
| US9650624B2 (en) | 2012-12-28 | 2017-05-16 | Butamax Advanced Biofuels Llc | DHAD variants for butanol production |
| US20160002131A1 (en) | 2013-02-21 | 2016-01-07 | Butamax Advanced Biofuels Llc | Vapor recompression |
| US9441250B2 (en) | 2013-03-14 | 2016-09-13 | Butamax Advanced Biofuels Llc | Glycerol 3- phosphate dehydrogenase for butanol production |
| CN105229156A (zh) | 2013-03-15 | 2016-01-06 | 布特马斯先进生物燃料有限责任公司 | 用于利用提取发酵生产丁醇的方法 |
| WO2014144643A1 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Method for producing butanol using extractive fermentation |
| WO2014151645A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Process for maximizing biomass growth and butanol yield by feedback control |
| US9580705B2 (en) | 2013-03-15 | 2017-02-28 | Butamax Advanced Biofuels Llc | DHAD variants and methods of screening |
| US9771602B2 (en) | 2013-03-15 | 2017-09-26 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
| CN105073991B (zh) * | 2013-03-28 | 2019-04-05 | 株式会社钟化 | 改变型羰基还原酶及其基因 |
| US9663759B2 (en) | 2013-07-03 | 2017-05-30 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| US20160264927A1 (en) | 2013-10-28 | 2016-09-15 | Danisco Us Inc. | Large Scale Genetically Engineered Active Dry Yeast |
| WO2015077752A1 (en) | 2013-11-25 | 2015-05-28 | Genomatica, Inc. | Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol |
| EP3077501B1 (en) | 2013-12-03 | 2021-07-28 | Genomatica, Inc. | Microorganisms and methods for improving product yields on methanol using acetyl-coa synthesis |
| US10280438B2 (en) | 2014-08-11 | 2019-05-07 | Butamax Advanced Biofuels Llc | Method for the production of yeast |
| KR101632642B1 (ko) | 2015-01-29 | 2016-06-22 | 씨제이제일제당 주식회사 | 신규한 프로모터 및 그의 용도 |
| BR112019009415A2 (pt) * | 2016-11-09 | 2019-07-30 | Danisco Us Inc | levadura com a produção de álcool melhorada |
| US10796788B2 (en) * | 2017-06-19 | 2020-10-06 | Academia Sinica | Structural determination of carbohydrates using special procedure and database of mass spectra |
| WO2019006263A1 (en) * | 2017-06-30 | 2019-01-03 | Dow Global Technologies Llc | COATING FOR ALDEHYDE REHABILITATION AND METHOD OF MANUFACTURE |
| TWI760548B (zh) * | 2018-08-16 | 2022-04-11 | 葡萄王生技股份有限公司 | 包含胚芽乳酸桿菌gkm3之組合物之延緩老化之用途 |
| TWI718402B (zh) * | 2018-08-16 | 2021-02-11 | 葡萄王生技股份有限公司 | 副乾酪乳酸桿菌gks6之活性物質、含其之組合物及其促進長壽之用途 |
| CN115838754A (zh) * | 2018-12-27 | 2023-03-24 | 中国医学科学院药物研究所 | 生产达玛烯二醇-ii糖苷的重组菌及其应用 |
| CN111826405B (zh) * | 2019-04-16 | 2022-09-27 | 中国科学院大连化学物理研究所 | 一种生物催化还原丙酮酸产d-乳酸的方法 |
| CN110982771B (zh) * | 2019-12-26 | 2021-05-28 | 江南大学 | 一种合成对羟基扁桃酸的方法 |
| CN110904061B (zh) * | 2019-12-28 | 2021-05-18 | 南京朗恩生物科技有限公司 | 一种热稳定性增强的醇脱氢酶突变体及其应用 |
| CN110951704B (zh) * | 2019-12-28 | 2021-07-27 | 南京朗恩生物科技有限公司 | 一种热稳定性增强的醇脱氢酶突变体及其序列 |
| WO2021178805A1 (en) * | 2020-03-06 | 2021-09-10 | Gevo, Inc. | Novel nkr variants for increased production of isobutanol |
| WO2025133860A1 (en) | 2023-12-21 | 2025-06-26 | Danstar Ferment Ag | Increased production of acetyl-phosphate and products derived therefrom in yeasts |
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|---|---|---|---|---|
| WO2010051527A2 (en) * | 2008-10-31 | 2010-05-06 | Gevo, Inc. | Engineered microorganisms capable of producing target compounds under anaerobic conditions |
| WO2011008924A2 (en) * | 2009-07-15 | 2011-01-20 | Butamax(Tm) Advanced Biofuels Llc | Recovery of butanol from a mixture of butanol, water, and an organic extractant |
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| CA2831130A1 (en) | 2012-09-27 |
| BR112013024328A2 (pt) | 2020-08-25 |
| JP6099623B2 (ja) | 2017-03-22 |
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| AU2017200439A1 (en) | 2017-02-16 |
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| AU2012230737A1 (en) | 2013-09-19 |
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| ZA201306708B (en) | 2015-01-28 |
| US9790521B2 (en) | 2017-10-17 |
| CA2831130C (en) | 2021-10-26 |
| WO2012129555A3 (en) | 2013-02-28 |
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