CN103650816A - Micro-cuttage and rapid-propagation technology of sweet-scented osmanthus - Google Patents
Micro-cuttage and rapid-propagation technology of sweet-scented osmanthus Download PDFInfo
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- CN103650816A CN103650816A CN201210355138.4A CN201210355138A CN103650816A CN 103650816 A CN103650816 A CN 103650816A CN 201210355138 A CN201210355138 A CN 201210355138A CN 103650816 A CN103650816 A CN 103650816A
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- cuttage
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- 238000005516 engineering process Methods 0.000 title abstract description 9
- 235000019082 Osmanthus Nutrition 0.000 title abstract description 7
- 241000333181 Osmanthus Species 0.000 title abstract description 7
- 238000005520 cutting process Methods 0.000 claims abstract description 32
- 239000011159 matrix material Substances 0.000 claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 239000002689 soil Substances 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 3
- 239000003292 glue Substances 0.000 claims abstract description 3
- 238000002156 mixing Methods 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 235000019362 perlite Nutrition 0.000 claims abstract description 3
- 239000010451 perlite Substances 0.000 claims abstract description 3
- 235000009566 rice Nutrition 0.000 claims abstract description 3
- 244000242564 Osmanthus fragrans Species 0.000 claims description 16
- 235000019083 Osmanthus fragrans Nutrition 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 9
- 230000001488 breeding effect Effects 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000009286 beneficial effect Effects 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 240000007643 Phytolacca americana Species 0.000 claims description 2
- 235000009074 Phytolacca americana Nutrition 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 claims description 2
- 239000010903 husk Substances 0.000 claims description 2
- 208000015181 infectious disease Diseases 0.000 claims description 2
- 230000000422 nocturnal effect Effects 0.000 claims description 2
- 230000000050 nutritive effect Effects 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims 2
- 229930192334 Auxin Natural products 0.000 claims 1
- 239000002363 auxin Substances 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 claims 1
- 235000019994 cava Nutrition 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 abstract description 3
- 108010051696 Growth Hormone Proteins 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 230000000249 desinfective effect Effects 0.000 abstract 2
- 239000000122 growth hormone Substances 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 230000003020 moisturizing effect Effects 0.000 abstract 1
- 238000010008 shearing Methods 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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Abstract
The invention belongs to the agricultural technology field, and particularly relates to a micro-cuttage and rapid-propagation technology of sweet-scented osmanthus. The micro-cuttage and rapid-propagation technology includes: selecting a plug tray of 40*40*45mm as a matrix vessel and constructing a support of covering films on the plug tray; mixing 30% of field and garden soil, 20% of plant ash, 20% of perlite and 30% of rice hull, using the mixture as a matrix, and disinfecting the matrix; selecting annual and lignified sweet-scented osmanthus branches as cuttings to be performed cuttage, and shearing the cuttings into small sections according to knot intervals, wherein each section is provided with only one bud knot and two leaves; cleaning and disinfecting the cuttings, and inserting the cuttings into the plug tray after performing glue enveloping on a section wound on each cutting; dropping growth hormone into each transplanting hole; sealing and moisturizing the plug tray. The micro-cuttage and rapid-propagation technology of the sweet-scented osmanthus greatly increases an expanding propagation multiple of cuttage propagation of the sweet-scented osmanthus by shortening the cuttings, enables survival rate of the cuttings to reach above 95% by improving the matrix of the plug tray and performing pretreatment before cuttage, and enables the rooting percentage of the cuttings to reach above 80% during one month by controlling temperature and humidity and processing the cuttings with the growth hormone.
Description
Technical field
The invention belongs to agricultural technology field, be specifically related to a kind of sweet osmanthus micro cuttage fast breeding technique.
Background technology
Sweet osmanthus is a kind of greening, beautify, the close combination of sweetening treatment three, views and admires and use the good Landscape Tree Species having both, traditional ornamental tree species of Ye Shi China.For a long time, Chinese scholars has been carried out comparatively extensively and the research of infiltrating sweet osmanthus, in recent years in the classification of osmanthus cultivars, a lot of research work has been done in the collection of germ plasm resource, breeding and culturing, gather processing and the aspect such as Landscape Application, obtained very large achievement.But the normal cutting propagation of sweet osmanthus is because being subject to the restriction of the factors such as time in season, area surroundings difference and sweet osmanthus growth rate, existence is taken root slowly, is grown slowly, expands numerous cycle length and expands the defects such as numerous multiple is low, especially for some precious improved seeds, because source is rare, cause delaying promoting.
Summary of the invention
The object of the present invention is to provide the sweet osmanthus cuttage and quick-propagation technology that the numerous coefficient of a kind of expansion is high, with low cost, simple and easy to do.Use the method can greatly improve the breeding rate of sweet osmanthus cuttage, and greatly shorten the required time of taking root.The present invention, by coil independently seedbed of conduct with cave, makes sweet osmanthus cuttage break away from the restriction of soil condition, can cultivate and grow seedlings whenever and wherever possible, due to the relative isolation between each slotting cave of cave dish, has also greatly limited the propagation of damage by disease and insect simultaneously; By shortening cutting length, reduce the contained joint number of cutting, thereby greatly increased cuttage, expand numerous breeding rate, it expands numerous multiple is the more than 5 times of normal cutting propagation, for the popularization of the rare precious osmanthus cultivars in source, has great using value.
Advantage of the present invention is:
1, expand numerous multiple high.Owing to having shortened cutting length, reduce the contained joint number of cutting, thereby greatly increased the availability of every sweet osmanthus branch, it expands numerous multiple is the more than 5 times of normal cutting propagation.
2, take root soon, survival rate is high.By soaking somatotropin, maintain higher humidity, about one month, rooting rate can be up to 80% in cutting, and one and a half months can reach 90%.Mutual isolation and the sterilization to matrix and cutting due to matrix in the dish of cave, can greatly reduce the propagation of disease.Due to the sealing to external world of cave dish phase, also greatly reduce the threat of insect pest simultaneously.
3, raw material is easy to get, with low cost.Do not need a large amount of early investments and preparation, do not need complicated equipment support yet.These all can purchase cave dish, matrix components, support and film on the market everywhere, and price is also very cheap.
4, method is simple, is easy to management.Whole cuttage process is simple, does not need advanced technology and complicated operation, just can complete the whole process from preparing to seal within one day.Due to independence and the sealing of whole cave dish, the later stage does not need too many management except squirting to add water.
Accompanying drawing explanation:
Fig. 1 is sealer cave dish schematic diagram.
Fig. 2 is the photo that cutting growth was taken root after 30 days.
Fig. 3 is the photo of cutting growth after 60 days.
In figure, 1 is film, and 2 is pole, and 3 is cave dish.
Specific embodiment:
(1) preparation of container: select the rigid plastics cave dish of 4*4*4.5cm as matrix container.For the ease of controlling temp. and humidity, whole cave dish is unsuitable excessive, should be at 1000～1500cm
2in.The surrounding of cave dish fix 6 poles be convenient to cuttage after overlay film, pole is mono-section of 10cm, 5mm place, top is curved in 90 °ly pokes film preventing.
(2) preparation of matrix: add 10% ash with 30% rural area soil and add 20% perlite and add 40% rice husk (through fermentation) and mix as matrix, matrix is inserted in Pan Cha cave, cave after fully mixing each component, matrix should not filled out to such an extent that overfill is beneficial to the isolation of each slotting cave matrix, thereby prevents that germ from passing through matrix and mutually infecting.Finally with 0.1% carbendazim solution, irrigate.
(3) pretreatment of cutting: select raw lignified sweet osmanthus branch then as treating cuttage bar, after adopting back, branch to be placed under flowing water and to be rinsed, then soak 1～2 hour with 0.1% carbendazim solution.Every branch is cut into the long segment of 1～2cm according to interval, upper end clip retains the distance of 2～3mm from axillalry bud, lower end clip at least retains the distance of 5mm from petiole, cut the rear wound to section in every section of cutting and carry out glue sealing processing to prevent the infection of germ.Cutting lower end is soaked after 5 minutes and is taken out for inserting in hormone solution.
(4) cuttage of cutting: during cuttage, the blade of all cuttings keeps towards same direction as far as possible, vertically inserts in matrix about 5mm by cutting dark, then with hand around slightly by tight.Inserted the carbendazim solution surface spray one time 0.1% with watering can, with film, whole cave dish has been covered to sealing afterwards.
(5) management after inserting: cultivate inserting in the booth that the careful cave dish of sealing is placed in the local of indoor non-direct sunlight or has film to cover.Daytime temperature should be controlled within 25～30 ℃, and nocturnal temperature is within 22～28 ℃.Humidity is surveyed once for every two days, remains on 85% left and right and is advisable.More than treating that root grows to 3cm, just can be transferred to nutritive cube the inside, suitably apply fertilizer and be beneficial to sprout.
Claims (4)
1. a sweet osmanthus micro cuttage fast breeding technique, its main implementation step is: the preparation of (1) container: select bore 40mmx40mm bottom 20x20mm capacity 40cc, the rigid plastics cave dish in 72 caves is as matrix container, and whole cave dish should be in 1000～1500cm2.The surrounding of cave dish fix 6 poles be convenient to cuttage after overlay film, pole is mono-section of 10cm, 5mm place, top is curved in 90 °ly pokes film preventing.(2) preparation of matrix: add 10% ash with 30% rural area soil and add 20% perlite and add 40% rice husk (through fermentation) and mix as matrix, matrix is inserted in Pan Cha cave, cave after fully mixing each component, matrix should not filled out to such an extent that overfill is beneficial to the isolation of each slotting cave matrix, thereby prevents that germ from passing through matrix and mutually infecting.Finally with 0.1% carbendazim solution, irrigate.(3) pretreatment of cutting: select raw lignified sweet osmanthus branch then as treating cuttage bar, after adopting back, branch to be placed under flowing water and to be rinsed, then soak 1～2 hour with 0.1% carbendazim solution.Every branch is cut into the long segment of 1～2cm according to interval, upper end clip retains the distance of 2～3mm from axillalry bud, lower end clip at least retains the distance of 5mm from petiole, cut the rear wound to section in every section of cutting and carry out glue sealing processing to prevent the infection of germ.Cutting lower end is soaked after 5 minutes and is taken out for inserting in hormone solution.(4) cuttage of cutting: during cuttage, the blade of all cuttings keeps towards same direction as far as possible, vertically inserts in matrix about 5mm by cutting dark, then with hand around slightly by tight.Inserted the carbendazim solution surface spray one time 0.1% with watering can, with film, whole cave dish has been covered to sealing afterwards.(5) management after inserting: be placed in the place of indoor non-direct sunlight or green house and cultivate inserting the careful cave dish of sealing.Daytime temperature should be controlled within 25～30 ℃, and nocturnal temperature is within 22～28 ℃.Humidity is surveyed once for every two days, remains on 85% left and right and is advisable.More than treating that root grows to 3cm, just can be transferred to nutritive cube the inside, suitably apply fertilizer and be beneficial to sprout.
2. a kind of sweet osmanthus micro cuttage fast breeding technique as claimed in claim 1, is characterized in that: every section, the branch through blocking saves (terminal bud can save containing two buds), two leaves containing a bud, and every leaf only stays 2～3cm
2, redundance is cut.
3. a kind of sweet osmanthus micro cuttage fast breeding technique as claimed in claim 1, is characterized in that: hormone used is auxin IAA (heteroauxin), and its concentration is 1.0mg/L.
4. a kind of sweet osmanthus micro cuttage fast breeding technique as claimed in claim 1, is characterized in that: after sealing, in the dish of cave, air humidity remains on 85% left and right, within every three days, surveys once, and overdrying, with watering can water spray, is crossed the wet film of just taking off and ventilated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210355138.4A CN103650816B (en) | 2012-09-21 | 2012-09-21 | A kind of method that sweet osmanthus micro cuttage is numerous soon |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210355138.4A CN103650816B (en) | 2012-09-21 | 2012-09-21 | A kind of method that sweet osmanthus micro cuttage is numerous soon |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103650816A true CN103650816A (en) | 2014-03-26 |
| CN103650816B CN103650816B (en) | 2016-04-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210355138.4A Active CN103650816B (en) | 2012-09-21 | 2012-09-21 | A kind of method that sweet osmanthus micro cuttage is numerous soon |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025833A (en) * | 2014-04-28 | 2014-09-10 | 深圳文科园林股份有限公司 | Sweet osmanthus cuttage rapid propagation technology |
| CN104186188A (en) * | 2014-09-17 | 2014-12-10 | 重庆市涪陵区外湾花卉种植园 | Cultivation method for Osmanthus fragrans |
| CN104322250A (en) * | 2014-10-14 | 2015-02-04 | 深圳文科园林股份有限公司 | Efficient propagation method for maintaining chimera pineapple character stable inheritance |
| CN104355892A (en) * | 2014-11-19 | 2015-02-18 | 柳州市潮林机械有限公司 | Special nutritional soil for osmanthus tree and preparation method thereof |
| CN104920025A (en) * | 2015-06-04 | 2015-09-23 | 湖北天健农产品有限公司 | Cutting breeding method |
| CN105009919A (en) * | 2015-08-06 | 2015-11-04 | 张剑逸 | Osmanthus fragrans cutting seedling method |
| CN105638358A (en) * | 2016-01-09 | 2016-06-08 | 徐尤双 | Cultivation method of sweet-scented osmanthus seedlings |
| CN106386071A (en) * | 2016-08-31 | 2017-02-15 | 界首市金玖江农业发展有限公司 | Cutting and seedling growing method for sweet osmanthus |
| CN106416670A (en) * | 2016-08-31 | 2017-02-22 | 界首市金玖江农业发展有限公司 | Osmanthus fragrans planting method |
| CN106614725A (en) * | 2016-10-12 | 2017-05-10 | 许伟琦 | Growth regulating agent for osmanthus tree seedling culture, plantation and transplantation |
| CN106973738A (en) * | 2016-01-15 | 2017-07-25 | 重庆晨之鑫生态农业发展有限公司 | The cuttage implantation methods of osmanthus fragrans |
| CN107188700A (en) * | 2017-06-22 | 2017-09-22 | 和县赭洛山茶叶种植专业合作社 | A kind of cuttage and seedling culture method of osmanthus fragrans |
| CN108283189A (en) * | 2018-02-08 | 2018-07-17 | 金华市飞凌生物科技有限公司 | A kind of wave leaf gold osmanthus cuttage root-taking agent |
| CN108925261A (en) * | 2018-06-13 | 2018-12-04 | 山西省农业科学院生物技术研究中心 | A kind of black fruit fructus lycii micro cuttage rapid propagation method |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104025833A (en) * | 2014-04-28 | 2014-09-10 | 深圳文科园林股份有限公司 | Sweet osmanthus cuttage rapid propagation technology |
| CN104186188A (en) * | 2014-09-17 | 2014-12-10 | 重庆市涪陵区外湾花卉种植园 | Cultivation method for Osmanthus fragrans |
| CN104322250A (en) * | 2014-10-14 | 2015-02-04 | 深圳文科园林股份有限公司 | Efficient propagation method for maintaining chimera pineapple character stable inheritance |
| CN104355892A (en) * | 2014-11-19 | 2015-02-18 | 柳州市潮林机械有限公司 | Special nutritional soil for osmanthus tree and preparation method thereof |
| CN104920025B (en) * | 2015-06-04 | 2018-05-11 | 湖北天健农产品有限公司 | Cottage breeding method |
| CN104920025A (en) * | 2015-06-04 | 2015-09-23 | 湖北天健农产品有限公司 | Cutting breeding method |
| CN105009919A (en) * | 2015-08-06 | 2015-11-04 | 张剑逸 | Osmanthus fragrans cutting seedling method |
| CN105638358A (en) * | 2016-01-09 | 2016-06-08 | 徐尤双 | Cultivation method of sweet-scented osmanthus seedlings |
| CN106973738A (en) * | 2016-01-15 | 2017-07-25 | 重庆晨之鑫生态农业发展有限公司 | The cuttage implantation methods of osmanthus fragrans |
| CN106416670A (en) * | 2016-08-31 | 2017-02-22 | 界首市金玖江农业发展有限公司 | Osmanthus fragrans planting method |
| CN106386071A (en) * | 2016-08-31 | 2017-02-15 | 界首市金玖江农业发展有限公司 | Cutting and seedling growing method for sweet osmanthus |
| CN106614725A (en) * | 2016-10-12 | 2017-05-10 | 许伟琦 | Growth regulating agent for osmanthus tree seedling culture, plantation and transplantation |
| CN107188700A (en) * | 2017-06-22 | 2017-09-22 | 和县赭洛山茶叶种植专业合作社 | A kind of cuttage and seedling culture method of osmanthus fragrans |
| CN108283189A (en) * | 2018-02-08 | 2018-07-17 | 金华市飞凌生物科技有限公司 | A kind of wave leaf gold osmanthus cuttage root-taking agent |
| CN108925261A (en) * | 2018-06-13 | 2018-12-04 | 山西省农业科学院生物技术研究中心 | A kind of black fruit fructus lycii micro cuttage rapid propagation method |
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| CN103650816B (en) | 2016-04-06 |
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Address after: Futian District Shennan Road Shenzhen city Guangdong province 518000 Shenzhen International Innovation Center A block 36 layer Applicant after: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Address before: 518048 Guangdong city of Shenzhen province Futian District Binhe Central Valley 21 storey building Applicant before: SHENZHEN WENKE LANDSCAPE Co.,Ltd. |
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Application publication date: 20140326 Assignee: Guangdong Yaoda Financial Leasing Co.,Ltd. Assignor: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Contract record no.: X2023980044229 Denomination of invention: A Method for Rapid Propagation of Osmanthus fragrans by Microcutting Granted publication date: 20160406 License type: Exclusive License Record date: 20231023 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A Method for Rapid Propagation of Osmanthus fragrans by Microcutting Effective date of registration: 20231024 Granted publication date: 20160406 Pledgee: Guangdong Yaoda Financial Leasing Co.,Ltd. Pledgor: SHENZHEN WENKE LANDSCAPE Co.,Ltd. Registration number: Y2023980062607 |