CN103650816A - Micro-cuttage and rapid-propagation technology of sweet-scented osmanthus - Google Patents

Abstract

The invention belongs to the field of agricultural technology, and in particular relates to a rapid propagation technology of osmanthus fragrans micro-cuttage. A hole plate of 40*40*45mm is selected as a matrix container, and a film-covered support is built on the hole plate; 30% of pastoral soil is used to add 20% Plant ash plus 20% perlite plus 30% rice husk is mixed as a matrix and sterilized; choose the lignified osmanthus branches of the year as cuttings, and cut them into small sections according to the internode distance, each section only contains one bud node and two leaves; The cuttings are cleaned and sterilized, and the cross-section wounds on the cuttings are sealed and then inserted into the hole tray; growth hormone is added dropwise to each hole; and the hole tray is sealed and moisturized. The Osmanthus osmanthus plug micro-cuttage rapid propagation technology of the present invention greatly increases the multiplication multiple of osmanthus fragrans cutting propagation by shortening the length of the cutting branches; by improving the plug substrate and pretreatment before cutting, the survival rate reaches 95%. Above, by controlling temperature and humidity and treating with growth hormone, its rooting rate can reach more than 80% within one month.

Description

A kind of osmanthus micro-cuttage rapid propagation technique

technical field

The invention belongs to the technical field of agriculture, and in particular relates to a rapid propagation technology of osmanthus fragrans micro-cuttage. the

Background technique

Osmanthus fragrans is an excellent garden tree species that is closely combined with greening, beautification and fragrance, and has both viewing and use. It is also a traditional ornamental tree species in my country. For a long time, domestic and foreign scholars have carried out relatively extensive and infiltrating research on osmanthus fragrans. In recent years, a lot of research work has been done on the classification of osmanthus fragrans varieties, collection of germplasm resources, propagation and cultivation, harvesting and processing, and garden application. Great results have been achieved. However, the conventional cutting of osmanthus is limited by factors such as seasonal time, regional environmental differences and osmanthus growth speed, and has defects such as slow rooting, slow growth, long propagation cycle and low propagation multiples, especially for some precious fine varieties. The lack of resources has led to delays in promotion. the

Contents of the invention

The object of the present invention is to provide a kind of rapid propagation technology of sweet-scented osmanthus cuttage with high propagation coefficient, low cost and easy to implement. Using this method can greatly increase the propagation multiple of osmanthus fragrans cuttings, and greatly shorten the time required for rooting. In the present invention, the cuttings of osmanthus fragrans are freed from the restriction of land conditions by using the plug trays as independent seedbeds, and can be used to cultivate and raise seedlings anytime and anywhere. At the same time, due to the relative isolation between the insertion holes of the plug trays, the spread of diseases and insect pests is also greatly limited; The length of the cuttings reduces the number of nodes contained in the cuttings, thereby greatly increasing the propagation multiple of cuttings, which is more than 5 times that of conventional cuttings. It has great application value for the promotion of precious osmanthus varieties with scarce sources. . the

The advantages of the present invention are:

1. High multiplication factor. Due to shortening the length of the cuttings and reducing the number of nodes contained in the cuttings, the utilization rate of each osmanthus fragrans branch is greatly increased, and its multiplication factor is more than 5 times that of conventional cuttings. the

2. Fast rooting and high survival rate. By soaking in growth hormone and maintaining high humidity, the rooting rate of cuttings can be as high as 80% in about one month, and 90% in one and a half months. Due to the isolation of the matrix in the plug tray and the disinfection of the matrix and cuttings, the spread of diseases can be greatly reduced. Due to the closure of the hole tray relative to the outside world, the threat of insect pests is also greatly reduced. the

3. The raw materials are easy to obtain and the cost is low. It does not require a lot of upfront investment and preparation, nor does it require complex equipment support. Trays, matrix components, scaffolds, and membranes are readily available and inexpensive. the

4. The method is simple and easy to manage. The whole cutting process is simple and does not require advanced technology and complicated operations, and the whole process from preparation to sealing can be completed within one day. Due to the independence and sealing of the entire tray, there is no need for much management in the later stage except for spraying and adding water. the

Description of drawings:

Figure 1 is a schematic diagram of the sealing film plug. the

Figure 2 is a photograph of rooting of cuttings after 30 days of growth. the

Figure 3 is a photo of the cuttings after 60 days of growth. the

In the figure, 1 is a film, 2 is a pole, and 3 is a tray. the

Specific examples:

(1) Preparation of the container: choose a 4*4*4.5cm hard plastic tray as the matrix container. In order to facilitate temperature and humidity control, the entire plug should not be too large, preferably within ^1000-1500cm2 . Fix 6 support rods around the hole plate to facilitate film covering after cutting. The support rods are 10 cm long, and the top 5 mm is bent into 90° to prevent the film from being punctured.

(2) Substrate preparation: use 30% pastoral soil plus 10% plant ash plus 20% perlite plus 40% rice husk (fermented) to mix as the substrate, and fill the substrate into the plug hole after fully mixing the components , the matrix should not be overfilled to facilitate the isolation of each insertion hole matrix, thereby preventing mutual infection of germs through the matrix. Finally, pour it with 0.1% carbendazim solution. the

(3) Pretreatment of cuttings: select the lignified sweet-scented osmanthus branches of the year as cuttings, put the branches under running water after harvesting, and then soak them with 0.1% carbendazim solution for 1 to 2 hours. Cut each branch into 1 to 2 cm long sections according to the internode distance, keep the distance between the upper cut and the axillary buds at 2 to 3 mm, and keep at least 5 mm between the lower cut and the petiole. Glue sealing treatment to prevent the infection of germs. The lower end of the cuttings is soaked in the hormone solution for 5 minutes and then taken out for preparation. the

(4) Cutting of cuttings: When cutting, keep the leaves of all cuttings in the same direction as much as possible, insert the cuttings vertically into the matrix to a depth of about 5mm, and then press the surroundings slightly with your hands. Spray 0.1% carbendazim solution once on the surface with a watering can after inserting, and then cover and seal the entire plug with a film. the

(5) Management after inserting: Place the plugged and sealed plug trays indoors in a place not exposed to direct sunlight or in a greenhouse covered with film for cultivation. The daytime temperature should be controlled within 25-30°C, and the nighttime temperature should be within 22-28°C. The humidity is measured every two days, and it is advisable to keep it at about 85%. When the roots grow to more than 3cm, they can be transferred to the nutrient bowl, and appropriate fertilizers are applied to facilitate sprouting. the

Claims (4)

1. a kind of Osmanthus fragrans micro-cuttage rapid propagation technology, its main implementation steps are: (1) the preparation of container: select the hard plastic tray of 40mmx40mm bottom 20x20mm capacity 40cc of diameter, 72 holes as matrix container, and whole tray should be in 1000 ~1500cm2 or less. Fix 6 support rods around the hole plate to facilitate film covering after cutting. The support rods are 10 cm long, and the top 5 mm is bent into 90° to prevent the film from being punctured. (2) Substrate preparation: use 30% pastoral soil plus 10% plant ash plus 20% perlite plus 40% rice husk (fermented) to mix as the substrate, and fill the substrate into the plug hole after fully mixing the components , the matrix should not be overfilled to facilitate the isolation of each insertion hole matrix, thereby preventing mutual infection of germs through the matrix. Finally, pour it with 0.1% carbendazim solution. (3) Pretreatment of cuttings: select the lignified sweet-scented osmanthus branches of the year as cuttings, put the branches under running water after harvesting, and then soak them with 0.1% carbendazim solution for 1 to 2 hours. Cut each branch into 1 to 2 cm long sections according to the internode distance, keep the distance between the upper cut and the axillary buds at 2 to 3 mm, and keep at least 5 mm between the lower cut and the petiole. Glue sealing treatment to prevent the infection of germs. The lower end of the cuttings is soaked in the hormone solution for 5 minutes and then taken out for preparation. (4) Cutting of cuttings: When cutting, keep the leaves of all cuttings in the same direction as much as possible, insert the cuttings vertically into the matrix to a depth of about 5mm, and then press the surroundings slightly with your hands. Spray 0.1% carbendazim solution once on the surface with a watering can after inserting, and then cover and seal the entire plug with a film. (5) Management after insertion: Place the plugged and sealed plugs in a place not exposed to direct sunlight indoors or in a greenhouse for cultivation. The daytime temperature should be controlled within 25-30°C, and the nighttime temperature should be within 22-28°C. The humidity is measured every two days, and it is advisable to keep it at about 85%. When the roots grow to more than 3cm, they can be transferred to the nutrient bowl, and appropriate fertilizers are applied to facilitate sprouting. 2. a kind of sweet-scented osmanthus micro-cuttage rapid propagation technology as claimed in claim 1 is characterized in that: every section of the branch only contains a bud node (terminal bud can contain two bud nodes), two leaves, each leaf Only 2-3cm ² leaves are left, and the excess part is cut off. 3. a kind of sweet-scented osmanthus micro-cuttage rapid propagation technology as claimed in claim 1 is characterized in that: used hormone is auxin IAA (indole acetic acid), and its concentration is 1.0mg/L. 4. a kind of sweet-scented osmanthus micro-cuttage quick-propagation technology as claimed in claim 1 is characterized in that: after sealing, the air humidity in the plug remains at about 85%, and it is measured once every three days. When wet, remove the film and ventilate.

Images