CN103563616A - Method for improving survival rate of temporarily planted sugarcane tissue culture seedling - Google Patents
Method for improving survival rate of temporarily planted sugarcane tissue culture seedling Download PDFInfo
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- CN103563616A CN103563616A CN201310470491.1A CN201310470491A CN103563616A CN 103563616 A CN103563616 A CN 103563616A CN 201310470491 A CN201310470491 A CN 201310470491A CN 103563616 A CN103563616 A CN 103563616A
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- sugarcane
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Abstract
The invention provides a method for improving the survival rate of a temporarily planted sugarcane tissue culture seedling. The method adopts the technical scheme that after 55-67% of fresh countryside dung is mixed with 35-43% of vegetable field mug, the mixture is fermented to be ripe for serving as low-layer material ingredients of a seedling bed of the sugarcane tissue culture seedling, the upper layer of the seedling bed is river sand, and in the temporary planting period of the sugarcane tissue culture seedling, a 60w incandescent light is used for manually lighting for 12 hours every night for prolonging the lighting time so as to enhance the growing capability of the sugarcane. The method is wide in source of raw materials, environment friendly, simple in operation, convenient to manage and easy to popularize and has an effect protecting the environment, the nutrition absorption of the sugarcane tissue culture seedling is effectively promoted, the rooting and growing of the sugarcane are promoted, the survival rate is doubly increased, and the production cost is reduced.
Description
Technical field
The present invention relates to cane seedling breeding production technology, particularly a kind of method that improves survival rate of grafting sugarcane group seedling.
Background technology
Sugarcane originates in tropical and subtropical region, and in the extensively plantation of these areas.The country of world's sugarcane acreage maximum is Brazil, is secondly India, the 3rd of China's rank.Sugarcane occupies critical role in China's agricultural economy, and its output and the output value are only second to grain, oil plant and cotton, occupies the 4th.Sugarcane or a kind of bio-energy crop in China of high yield.Since the nineties in 20th century, shift to west south in China's sugar material base, has formed Guangxi, Yunnan, Guangdong, Si great main producing region, Xinjiang.And Guangxi is the main producing region of national sugarcane.Current, Guangxi Sugarcane Industry developmental research has become hot issue.Guangxi Sugarcane Industry development rapidly, becomes the first sugar industry of China base.Reinforcement comprises the research of Sugarcane Industry development to sugarcane each side, significant for accelerating society of Guangxi, politics, expanding economy.
The sugarcane in Guangxi is the highest with the annual production of guest sugarcane again, and annual production in 2012 is up to 1,000 ten thousand tons.Adopt the sugarcane sugarcane quality that directly method of plantation cultivates in place not good, to the coming year sugarcane kind quality influence quite dark.At present, the sugarcane kind of next year, by the mode of cultivation sugar-cane tissue culture seedlings, is cultivated in most of cane plantings family, and it is higher that the sugarcane of pointing out in gained sugarcane kind produces sugar, can stay sugarcane kind quality better the coming year, hence one can see that, and survival rate of grafting sugarcane group seedling directly has influence on the output of sugarcane then.At present, sugarcane tissue-culture shoot survival percent is on the low side, what be medium people by research, and the NAA medium of 4～5mg/L is got in discovery, the method that the paclobutrazol of 2～3mg/L, composition upper strata, seedbed Wei Sha, lower floor are earth can improve survival rate of grafting sugarcane group seedling, up to more than 75%.The people such as Tang Junhai disclose a kind of sugarcane tissue culture seedling provisonal planting method, and the method is for low temperature, and in cultivating canopy, utilizing power is that the infrared radiation type bulb of 250～500w carries out artificial lighting 10～14h.Thereby improved sugarcane tissue-culture shoot survival percent, the method makes survival rate of grafting sugarcane group seedling reach 85%.Visible, these two kinds of method gained sugarcane tissue-culture shoot survival percents or relatively low.
Therefore, how to develop and can improve mode that survival rate of grafting sugarcane group seedling uses again comparatively environmental protection and cultivate sugar-cane tissue culture seedlings and reduce and cultivate cost and become a problem demanding prompt solution.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sugarcane tissue culture seedling provisonal planting breeding method, to improve survival rate of grafting sugarcane group seedling, reduces seedling cost, reduces labour intensity.
For solving the problems of the technologies described above, the invention provides following technical scheme:
Improve survival rate of grafting sugarcane group seedling method, after adopting 55%～67% fresh farmers' excrement to mix with 35%～43% field of vegetables mud, macerate ripely, for sugar-cane tissue culture seedlings seedbed lower floor material component, upper strata, seedbed is river sand.Preferably, described farmers''s excrement comprises: human and animal excreta, poultry manure.
Preferably, under described group of training seedling seedbed, composition of layer is 60% fresh human and animal excreta, 40% field of vegetables mud, after retting maturation, makes.
Preferably, under described group of training seedling seedbed, composition of layer is 67% fresh poultry manure, 33% field of vegetables mud, after retting maturation, makes.
Preferably, composition of layer under described sugarcane tissue culture seedling provisonal planting seedbed, retting 2～3 months.
Preferably, use evening 60w incandescent lamp directly to shine 12 hours seedbed.
Beneficial effect of the present invention:
The present invention uses raw material sources extensive, and environmental protection can effectively promote sugar-cane tissue culture seedlings nutrient absorption, impels the sugarcane growth of taking root, survival rate multiplication.Booth adopts incandescent bulb, increases sugar-cane tissue culture seedlings light application time, thereby strengthens sugarcane production ability, reaches and cultivates strong sprout, improves survival rate of grafting sugarcane group seedling.This invention has reduced production cost, simple to operate, and convenient management is easy to promote.Environment has also been played to protective effect simultaneously.
Embodiment
Referring to embodiment, further describe the present invention, to make those skilled in the art can implement according to this with reference to specification word, protection domain of the present invention is not limited by embodiments of the present invention.
The new platform sugar of take is for No. 22 confession examination material, start to organize cultivation October, obtain sugarcane callus, allow cell continue division growth, after 3 months, proceed to the root media that contains 1/2MS+NAA4mg/L+ paclobutrazol 2mg/L+ sucrose 70g/L and carry out culture of rootage, after 10 days, grow flourishing root system and obtain bottle seedling of taking root.Build seedling canopy, build a long 7m, wide 5m, the booth of high 2.5m, booth covers completely with large film, with earth, brick, presses corner, then hides top with black sunshade.Minute 4 huts in booth, specification is: long 3m, wide 2m, high 2m.Hut does not bind, and surrounding makes a circle with half meter of high white film, with earth, finger stone, presses corner.4 huts are numbered respectively first, second, third, fourth.
Survival rate of grafting sugarcane group seedling=heel in survives a number/heel in sugar-cane tissue culture seedlings number * 100%
Embodiment 1
With 60% fresh human and animal excreta, 40% field of vegetables mud, mix, in a huddle, be put into the excrement room of sun-shading rain-proof, through 3 months rettings.After maturation, this mixture is filled out and be layered in above-mentioned hut first, thickness is 750mm.Above-mentioned bottle seedling of taking root is taken out, and water rinses 2 minutes, and after handling well, each clump is divided into 5 strains, heels on ready-made seedbed, and requiring insertion depth is 10mm.Each strain interval 40mm heels in.Heeled in after sugarcane young plant, then in kind, had on the seedbed of sugarcane young plant and spread the river sand that thickness is 5mm, then with 1000 times of thiophanate methyl dilutions, watered pouring.Every night, 18:30:00～06:30, carried out lamp photograph with 60w incandescent lamp.During this time, carry out the measure to booth ventilation, after 10 days, supplement urea, potash fertilizer regularly to every 4 days sugarcane seedling spray, spray altogether after 4 times, heel in sugarcane seedling and prepare out nursery.Heel in after 15 days, raise nursery film around, carry out hardening, Second Year can go out nursery at the beginning of 3 months.Carry out experimental record, calculate survival rate of grafting sugarcane group seedling.During sugarcane tissue-culture nursery is heeled in, carry out artificial lighting with lamp every night, increases light application time, thereby strengthen sugarcane production ability.
Embodiment 2
With 67% new freshly-slaughtered poultry, duck ight soil, 33% field of vegetables mud, mix, in a huddle, be put into the excrement room of sun-shading rain-proof, through 2 months rettings.After maturation, this mixture is filled out and be layered in above-mentioned hut second, thickness is 750mm.Above-mentioned bottle seedling of taking root is taken out, and water rinses 2 minutes, and after handling well, each clump is divided into 5 strains, heels on ready-made seedbed, and requiring insertion depth is 10mm.Each strain interval 40mm heels in.Heeled in after sugarcane young plant, then in kind, had on the seedbed of sugarcane young plant and spread the river sand that thickness is 5mm, then with 1000 times of thiophanate methyl dilutions, watered pouring.Every night, 18:30:00～06:30, carried out lamp photograph with 60w incandescent lamp.During this time, carry out the measure to booth ventilation, after 10 days, supplement urea, potash fertilizer regularly to every 4 days sugarcane seedling spray, spray altogether after 4 times, heel in sugarcane seedling and prepare out nursery.Heel in after 15 days, raise nursery first film around, carry out hardening, Second Year can go out nursery at the beginning of 3 months.Carry out experimental record, calculate survival rate of grafting sugarcane group seedling.
Embodiment 3
With 55% fresh human and animal excreta, 45% field of vegetables mud, mix, in a huddle, be put into the excrement room of sun-shading rain-proof, through 3 months rettings.After maturation, this mixture is filled out and be layered in above-mentioned hut third, thickness is 750mm.Above-mentioned bottle seedling of taking root is taken out, and water rinses 2 minutes, and after handling well, each clump is divided into 5 strains, heels on ready-made seedbed, and requiring insertion depth is 10mm.Each strain interval 40mm heels in.Heeled in after sugarcane young plant, then in kind, had on the seedbed of sugarcane young plant and spread the river sand that thickness is 5mm, then with 1000 times of thiophanate methyl dilutions, watered pouring.Every night, 18:30～06:30, carried out lamp photograph with 60w incandescent lamp.During this time, carry out the measure to booth ventilation, after 10 days, supplement urea, potash fertilizer regularly to every 4 days sugarcane seedling spray, spray altogether after 4 times, heel in sugarcane seedling and prepare out nursery.Heel in after 15 days, raise nursery first film around, carry out hardening, Second Year can go out nursery at the beginning of 3 months.Carry out experimental record, calculate survival rate of grafting sugarcane group seedling.
Embodiment 4
With the field of vegetables mud disinfecting, fill out and be layered in above-mentioned hut fourth, thickness is 750mm.Above-mentioned bottle seedling of taking root is taken out, and water rinses 2 minutes, and after handling well, each clump is divided into 5 strains, heels on ready-made seedbed, and requiring insertion depth is 10mm.Each strain interval 40mm heels in.Heeled in after sugarcane young plant, then in kind, had on the seedbed of sugarcane young plant and spread the river sand that thickness is 5mm, then with 1000 times of thiophanate methyl dilutions, watered pouring.During this time, carry out the measure to booth ventilation, after 10 days, supplement urea, potash fertilizer regularly to every 4 days sugarcane seedling spray, spray altogether after 4 times, heel in sugarcane seedling and prepare out nursery.Heel in after 15 days, raise nursery first film around, carry out hardening, Second Year can go out nursery at the beginning of 3 months.Carry out experimental record, calculate survival rate of grafting sugarcane group seedling.
Data analysis
| Hut numbering | Survival rate of grafting sugarcane group seedling |
| First | 96.79% |
| Second | 97.04% |
| Third | 96.13% |
| Fourth | 90.48% |
As seen from the above table, under sugarcane tissue culture seedling provisonal planting seedbed, composition of layer is that farmers''s excrement mixes with field of vegetables mud the material that retting forms, be equipped with the method for heeling in of incandescent light photograph evening, than only with field of vegetables mud as composition of layer under seedbed and do not have evening illumination to heel in survival rate much high.
Claims (6)
1. a method that improves survival rate of grafting sugarcane group seedling, is characterized in that: under sugarcane tissue culture seedling provisonal planting used seedbed, composition of layer is 55%～67% fresh farmers' excrement, 33%～43% field of vegetables mud, after retting maturation, makes; Seedbed upper component is river sand.
2. the method for raising survival rate of grafting sugarcane group seedling according to claim 1, is characterized in that: described farmers''s excrement comprises: human and animal excreta, poultry manure.
3. the method for raising survival rate of grafting sugarcane group seedling according to claim 1, is characterized in that: under sugarcane tissue culture seedling provisonal planting used seedbed, composition of layer is 60% fresh human and animal excreta, 40% field of vegetables mud, after retting maturation, makes.
4. the method for raising survival rate of grafting sugarcane group seedling according to claim 1, is characterized in that: under sugarcane tissue culture seedling provisonal planting used seedbed, composition of layer is 67% fresh poultry manure, 33% field of vegetables mud, after retting maturation, makes.
5. the method for raising survival rate of grafting sugarcane group seedling according to claim 1, is characterized in that: composition of layer under described sugarcane tissue culture seedling provisonal planting seedbed, retting 2～3 months.
6. the method for raising survival rate of grafting sugarcane group seedling according to claim 1, is characterized in that: use evening 60w incandescent lamp directly to shine 12 hours seedbed.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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- 2013-10-10 CN CN201310470491.1A patent/CN103563616A/en active Pending
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| CN1985571A (en) * | 2006-12-15 | 2007-06-27 | 中国科学院沈阳应用生态研究所 | Seedling growing process for raising the survival rate of transplanted seedling tree |
| CN103069981A (en) * | 2013-01-10 | 2013-05-01 | 宁波大学 | Method for improving transplanting survival rate of catharanthus roseus tissue culture seeding |
| CN103238436A (en) * | 2013-05-11 | 2013-08-14 | 中国热带农业科学院热带生物技术研究所 | Aperture disk transplanting provisonal planting method of original sugarcane detoxification seedlings |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
| CN113545268B (en) * | 2021-08-13 | 2022-09-09 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
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Application publication date: 20140212 |