CN103444608A - Reproductive regulation method of wolf eel parents - Google Patents
Reproductive regulation method of wolf eel parents Download PDFInfo
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- CN103444608A CN103444608A CN2013104159686A CN201310415968A CN103444608A CN 103444608 A CN103444608 A CN 103444608A CN 2013104159686 A CN2013104159686 A CN 2013104159686A CN 201310415968 A CN201310415968 A CN 201310415968A CN 103444608 A CN103444608 A CN 103444608A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a reproductive regulation method of wolf eel parents, and belongs to the technical field of aquaculture. The method comprises the following steps of selecting parents, cultivating parent fish, inducting gonad development and obtaining germ cells; after regulation and control are performed by adopting the technical scheme of the method, 6-year-old female wolf eels and 5-year-old male wolf eels can copulate and breed, so that the copulation and breeding are earlier for 1 year and two years respectively than that of the parents which live under the natural conditions and copulate and breed at the age of 7; the difficult technical problem that development of a female wolf eel and a male wolf eel is out of sync, and synchronous egg reproduction is realized; the reproductive habits of a pair of female wolf and male wolf eel, such as living in the same place, living in a cave, and protecting eggs are satisfied through placing a PVC plastic pipe, the success rate of development and cultivation of the parent gonad reaches 85%, and the fertilization rate reaches 80%.
Description
Technical field
The invention belongs to technical field of aquaculture, relate to particularly a kind of genital regulating method of wolf eel parent.
Background technology
Wolf eel (Anarrhichthys ocellatus Ayres, 1855) belong to Perciformes (Perciformes), wolf Blenniidae section (Anarhichadidae), eel wolffish genus (Anarrhichthys), English name Wolf-eel, Chinese name wolf eel, wolf eel, imperial eel etc., be distributed widely in northern marine site, the Pacific Ocean, the West Coast that sees the sea of Japan, Russia, Nan An, Aleutians, Alaska, America & Canada is Arctic Sea even.The wolf eel belongs to abyssal fishes, inhabits seabed, also can adapt to the intertidal zone hermatolith environment of coastal region.Young wolf eel lives in more shallow waters, after growth, just can transfer in darker under water cave and live.
Supporting kind with current China seawater industrial culturing master compares, the wolf eel has individuality, and (ripe individual body length can reach 2～2.5 meters greatly, body weight can reach 15～20 kilograms), fast growth (can grow more than 5 kilograms in 3～5 years, increase every year on average more than 1 kilogram), (low temperature can tolerate 0.5 ℃ to wide accommodation, high temperature can tolerate 24 ℃, salinity tolerance range 4～34), cultivation density large (cultivation density can reach 30～50 kilograms), delicious flavour, the characteristics such as nutritious, DEVELOPMENT PROSPECT is wide.
2011 and Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science in 2012 take the lead in having carried out wolf eel life habit and reproductive characteristic research, result shows, propagating artificially under condition, if the wolf eel does not carry out genital regulating, can be because of temperature drift or on the low side and the difference variation amplitude is excessive, cause sexual gland agensis or male and female to grow asynchronous, and can't obtain fertilized egg.Within 2013, previous experiences is summed up by project team; perfect wolf eel parent genital regulating technology, and take the lead in having broken through wolf eel seed propagation in scale technology, cultivate wolf eel commodity seed 5.2 ten thousand tails; the seed rearing survival rate reaches 55%, has set up a set of perfect wolf eel propagation in scale technical system.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of genital regulating method of wolf eel parent, the invention technical scheme is by the wolf eel is carried out to genital regulating, solves and grows asynchronous, a pair of male and female live together a place, the technical problem such as live in caves in evening wolf eel sexual maturity time, male and female.
A kind of genital regulating method of wolf eel parent, comprise parent selection, parent fish rearing, induce gonad development and fertilized egg to obtain, and concrete steps are as follows:
1. parent is selected
The bodily form is complete, and color and luster is normal, and health is energetic, ingests good, and milter is more than 5 ages, and body length is more than 120 centimetres, and body weight is more than 1600g; Raun is more than 6 ages, and female fish length is more than 140 centimetres, more than body weight 2000 grams;
2. parent fish rearing
2.1 density is controlled
Density is controlled at 2～3 tails/m
2, female-male proportion is 1:1.
2.2 fortification
Accelerating phase bait be take oyster meat, yellow croaker and scomberomorus niphonius fish piece as main, throws something and feeds every day 2 times, and day throwing amount of raising is 2%～3% of fish body weight; Egg-laying period bait be take and lived clam worm and little yellow croaker piece as main, and the yellow croaker piece adds the lecithin of 3～5 ‰ B B-complex and 1～2 ‰ to promote the parent gonad development before throwing something and feeding;
2.3 temperature is controlled
When natural temperature is down to 4～5 ℃, constant temperature 7～10 days, then rise 0.5 ℃ in every 3 days, and after temperature rises to 10 ℃, constant temperature is cultivated;
2.4 illumination is controlled
Intensity of illumination is 600～800Lx, and light application time changes with the variation of water temperature, and when water temperature is down to 4～5 ℃, light application time is 12h, keeps 7～10 days, then within every 3 days, extends 20min, and when illumination extends to 14h, permanent light is cultivated;
2.5 things concealed is selected
In the parents culture pond, place the PVC plastic tube as wolf eel things concealed, lay eggs and protect the ovum place;
Further, the quantity of described PVC plastic tube is identical with female parent stock, and the pipe surrounding is fixed with stone, with anti-rolling.
Further, the internal diameter of described PVC plastic tube is 110mm, and length is 30～40cm.
2.6 daily management
Change water 300%～400% every day, continue blowing aeration, keep dissolved oxygen more than 5mg/L;
3. induce gonad development
Check wolf eel raun gonad development situation, gonad development is hastened parturition to the raun of IV phase, milter can be extruded milky seminal fluid, does not need to hasten parturition.
Further, described wolf eel raun induced spawning method adopts intramuscular injection hormone method, adopt 80～100ppm eugenol anesthesia, 5～10min before the injection hormone, after its muscle tone is lost, injection Luteinizing hormone releasing-hormone A2 (being called for short LHRH-A2) 10～20 μ g/kg are hastened parturition.
Further, described wolf eel raun induced spawning method is hastened parturition for the LHRH-A2 that adds 20～30 μ g/kg in female fish meal.
4. fertilized egg obtains
The aftereffect of the hastening parturition time is 42～50 hours, can obtain fertilized egg by the mode of natural spawning.Wolf eel fertilized egg is viscid egg, and one, the sticky work of the fertilized egg after output is attached on pvc pipe.The wolf eel also has the ovum of protecting behavior in addition, and the parent circle becomes a circle, in the middle of fertilized egg is trapped among, is hatched.
The present invention's beneficial effect compared with prior art:
The invention provides a kind of wolf eel genital regulating technology, after being regulated and controled by technical scheme of the present invention, wolf eel raun can carry out the mating breeding 6 ages, milter in 5 ages, than parent under natural conditions until 7 ages could mating time of breeding shifted to an earlier date respectively 1 year and 2 years; Solve wolf eel male and female and grown asynchronous technical barrier, reached the synchronous spawning breeding; By throw in the PVC plastic tube met a pair of male and female live together a place, live in caves, protect the reproductive habit such as ovum, the parent gonad development is cultivated success rate and is reached 85%, fertilization rate 80%.
Embodiment
Below by embodiment, technical scheme of the present invention is further explained, but protection scope of the present invention is not subject to any pro forma restriction of embodiment.
Embodiment 1
In December, 2012, we carried out genital regulating work in development zone, Yantai Yi Jia plant to wolf eel parent, successful regulation and control and induce the parent gonadal maturation and obtained high quality fertilized egg of scophthatmus, and concrete steps are as follows:
1. parent is selected
Select wolf eel raun, each 50 tails of milter, selection criteria is that the bodily form is complete, color and luster normal, healthy, energetic, ingest well.Wolf eel parent is until could breed for 7 ages in mating under field conditions (factors), and the sexual maturity time is more late.Therefore under cultivating condition, milter should be more than 5 ages, and body length is more than 120 centimetres, and body weight is more than 1600g; Raun is more than 6 ages, and female fish length is more than 140 centimetres, more than body weight 2000 grams.
2. parent fish rearing
2.1 density is controlled
Be controlled at 2～3 tails/m
2, because the wolf eel has monogamy, female-male proportion is 1:1.
2.2 fortification
Accelerating phase bait be take oyster meat, yellow croaker and scomberomorus niphonius fish piece as main, throws something and feeds every day 2 times, and day throwing amount of raising is 2%～3% of fish body weight.Egg-laying period bait be take and lived clam worm and little yellow croaker piece as main, and the yellow croaker piece adds the lecithin of 3～5 ‰ B B-complex and 1～2 ‰ before throwing something and feeding, to promote the parent gonad development.
2.3 temperature is controlled
When natural temperature is down to 4～5 ℃, keep constant temperature 7～10 days, so often within latter 3 days, rise 0.5 ℃, after temperature rises to 10 ℃, constant temperature is cultivated.
2.4 illumination is controlled
The wolf eel belongs to the type fish that lay eggs spring, and finally reaching maturity of parent needs a process that illumination extends gradually.After starting regulation and control, parents culture pond surrounding is blocked with gobo, and 4 of the incandescent lamp bulbs of 100w evenly are installed dried up 0.4～0.6m eminence, and intensity of illumination is 600～800Lx.The regulation and control light application time changes with the variation of water temperature.When water temperature is down to 4～5 ℃, light application time is 6:00～18:00 12h altogether, keeps 7～10 days, within later every 3 days, extends backward 20min, keeps light application time to be total to 14h at 6:00～22:00, and permanent light is cultivated.
2.5 things concealed is selected
The wolf eel has a pair of male and female characteristic that a place lives in caves of living together, and laying the internal diameter identical with female parent stock in cultivating pond is 110mm, the PVC plastic tube that length is 30～40cm as wolf eel things concealed, lay eggs and protect the ovum place.The pipe surrounding is fixed with stone, with anti-rolling.
2.6 daily management
Change water 300%～400% every day, continue blowing aeration, keep dissolved oxygen more than 5mg/L; Every day, clear pond was 1 time, and to remove residual bait and ight soil, water temperature is measured in not timing, prevents that the fluctuating temperature amplitude is excessive.Make regular check on parent gonad development situation, the analysis-by-synthesis regulating effect.
3. induce gonad development
The production practices discovery, the wolf eel is difficult to be issued to final Maturation and spawning at artificial regulatory method.After the genital regulating of 3 months, wolf eel parent sexual gland is obviously grown, and checks raun gonad development situation, and gonad development to the female parent population of IV phase is hastened parturition.Because the wolf eel is more violent, adopt 80ppm eugenol anesthesia 10min before the injection hormone, after its muscle tone is lost, LH injection RH-A210 μ g/kg is hastened parturition; The LHRH-A2 that perhaps adds 20～30 μ g/kg in female fish meal is hastened parturition.Milter can be extruded milky seminal fluid under artificial regulatory, does not generally carry out hormone induction.
The aftereffect of the hastening parturition time is 50 hours, and wolf eel parent natural spawning, ejaculation obtain fertilized egg.Wolf eel fertilized egg is viscid egg, and one, the sticky work of the fertilized egg after output is attached on pvc pipe.The wolf eel also has the ovum of protecting behavior in addition, and the parent circle becomes a circle, in the middle of fertilized egg is trapped among, is hatched.
Final process statistics wolf eel parent gonad development is cultivated success rate and is reached 85%, fertilization rate reached 80%, and the incubation rate of fertilized egg under the artificial incubation condition reaches 90%, and the seed rearing survival rate reaches 55%.
Claims (5)
1. the genital regulating method of a wolf eel parent, comprise parent selection, parent fish rearing, induce gonad development and fertilized egg to obtain, and it is characterized in that concrete steps are as follows:
1) parent is selected
The bodily form is complete, and color and luster is normal, and health is energetic, ingests good, and milter is more than 5 ages, and body length is more than 120 centimetres, and body weight is more than 1600g; Raun is more than 6 ages, and female fish length is more than 140 centimetres, more than body weight 2000 grams;
2) parent fish rearing
2.1 density is controlled
Density is controlled at 2～3 tails/m
2, female-male proportion is 1:1;
2.2 fortification
Accelerating phase bait be take oyster meat, yellow croaker and scomberomorus niphonius fish piece as main, throws something and feeds every day 2 times, and day throwing amount of raising is 2%～3% of fish body weight; Egg-laying period bait be take and lived clam worm and little yellow croaker piece as main, and the yellow croaker piece adds the lecithin of 3～5 ‰ B B-complex and 1～2 ‰ to promote the parent gonad development before throwing something and feeding;
2.3 temperature is controlled
When natural temperature is down to 4～5 ℃, constant temperature 7～10 days, then rise 0.5 ℃ in every 3 days, and after temperature rises to 10 ℃, constant temperature is cultivated;
2.4 illumination is controlled
Intensity of illumination is 600～800Lx, and light application time changes with the variation of water temperature, and when water temperature is down to 4～5 ℃, light application time is 12h, keeps 7～10 days, then within every 3 days, extends 20min, and when illumination extends to 14h, permanent light is cultivated;
2.5 things concealed is selected
In the parents culture pond, place the PVC plastic tube as wolf eel things concealed, lay eggs and protect the ovum place;
2.6 daily management
Change water 300%～400% every day, continue blowing aeration, keep dissolved oxygen more than 5mg/L;
3) induce gonad development
Check wolf eel raun gonad development situation, gonad development is hastened parturition to the raun of IV phase, milter can be extruded milky seminal fluid, does not need to hasten parturition;
4) fertilized egg obtains
The aftereffect of the hastening parturition time is 42～50 hours, can obtain fertilized egg by the mode of natural spawning.
2. the genital regulating method of a kind of wolf eel parent according to claim 1, is characterized in that the quantity of described PVC plastic tube is identical with female parent stock, and the pipe surrounding is fixed with stone, with anti-rolling.
3. the genital regulating method of a kind of wolf eel parent according to claim 1, the internal diameter that it is characterized in that described PVC plastic tube is 110mm, length is 30～40cm.
4. the genital regulating method of a kind of wolf eel parent according to claim 1, it is characterized in that described wolf eel raun induced spawning method adopts intramuscular injection hormone method, adopt 80～100ppm eugenol anesthesia, 5～10min before the injection hormone, after its muscle tone is lost, injection Luteinizing hormone releasing-hormone A2 10～20 μ g/kg are hastened parturition.
5. the genital regulating method of a kind of wolf eel parent according to claim 1, is characterized in that described wolf eel raun induced spawning method is hastened parturition for the Luteinizing hormone releasing-hormone A2 that adds 20～30 μ g/kg in female fish meal.
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| CN107810887A (en) * | 2017-11-03 | 2018-03-20 | 浙江海洋大学 | A kind of hickie cuttlefish forced ripening method and proprietary reagent |
| CN107926780A (en) * | 2017-11-17 | 2018-04-20 | 上海市水产研究所 | A kind of spot Ji embryonated egg preparation methods |
| CN110199922A (en) * | 2019-07-05 | 2019-09-06 | 深圳华大海洋科技有限公司 | A method of silver eel being changed by yellow eel for induction Female Japanese eel |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107810887A (en) * | 2017-11-03 | 2018-03-20 | 浙江海洋大学 | A kind of hickie cuttlefish forced ripening method and proprietary reagent |
| CN107926780A (en) * | 2017-11-17 | 2018-04-20 | 上海市水产研究所 | A kind of spot Ji embryonated egg preparation methods |
| CN110199922A (en) * | 2019-07-05 | 2019-09-06 | 深圳华大海洋科技有限公司 | A method of silver eel being changed by yellow eel for induction Female Japanese eel |
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| CN103444608B (en) | 2015-02-04 |
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