CN103444608B - Reproductive regulation method of wolf eel parents - Google Patents
Reproductive regulation method of wolf eel parents Download PDFInfo
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- CN103444608B CN103444608B CN201310415968.6A CN201310415968A CN103444608B CN 103444608 B CN103444608 B CN 103444608B CN 201310415968 A CN201310415968 A CN 201310415968A CN 103444608 B CN103444608 B CN 103444608B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a reproductive regulation method of wolf eel parents, and belongs to the technical field of aquaculture. The method comprises the following steps of selecting parents, cultivating parent fish, inducting gonad development and obtaining germ cells; after regulation and control are performed by adopting the technical scheme of the method, 6-year-old female wolf eels and 5-year-old male wolf eels can copulate and breed, so that the copulation and breeding are earlier for 1 year and two years respectively than that of the parents which live under the natural conditions and copulate and breed at the age of 7; the difficult technical problem that development of a female wolf eel and a male wolf eel is out of sync, and synchronous egg reproduction is realized; the reproductive habits of a pair of female wolf and male wolf eel, such as living in the same place, living in a cave, and protecting eggs are satisfied through placing a PVC plastic pipe, the success rate of development and cultivation of the parent gonad reaches 85%, and the fertilization rate reaches 80%.
Description
Technical field
The invention belongs to technical field of aquaculture, relate to a kind of genital regulating method of wolf eel parent particularly.
Background technology
Wolf eel (Anarrhichthys ocellatus Ayres, 1855) Perciformes (Perciformes), wolf Blenniidae section (Anarhichadidae), eel wolffish genus (Anarrhichthys) is belonged to, English name Wolf-eel, Chinese name wolf eel, wolf eel, imperial eel etc., be distributed widely in northern marine site, the Pacific Ocean, see the West Coast even Arctic Sea of the sea of Japan, Russia, south, Alaska bank, Aleutians, America & Canada.Wolf eel belongs to abyssal fishes, inhabits seabed, also can adapt to the intertidal zone hermatolith environment of coastal region.Young wolf eel lives in more shallow waters, just can transfer in cave darker under water and live after growth.
Compared with supporting kind with current China seawater industrial culturing master, wolf eel has individuality, and (ripe individual body length can reach 2 ~ 2.5 meters greatly, body weight can reach 15 ~ 20 kilograms), fast growth (can grow more than 5 kilograms in 3 ~ 5 years, every year on average increase by more than 1 kilogram), (low temperature can tolerate 0.5 DEG C to wide accommodation, high temperature can tolerate 24 DEG C, salinity tolerance range 4 ~ 34), cultivation density large (cultivation density can reach 30 ~ 50 kilograms), delicious flavour, the feature such as nutritious, DEVELOPMENT PROSPECT is wide.
2011 and Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science in 2012 take the lead in having carried out wolf eel life habit and reproductive characteristic research, result shows, propagating artificially under condition, if wolf eel does not carry out genital regulating, then can because of temperature drift or on the low side and difference variation amplitude is excessive, cause sexual gland agensis or male and female to grow asynchronous, and cannot fertilized egg be obtained.Within 2013, previous experiences is summed up by project team; perfect wolf eel parent genital regulating technology, and take the lead in breaching wolf eel seed propagation in scale technology, cultivate wolf eel commodity seed 5.2 ten thousand tail; seed rearing survival rate, to 55%, establishes a set of perfect wolf eel propagation in scale technical system.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of genital regulating method of wolf eel parent, invention technical scheme, by carrying out genital regulating to wolf eel, solves evening wolf eel sexual maturity time, male and female grows asynchronous, a pair male and female and to live together a place, the technical problem such as to live in caves.
A genital regulating method for wolf eel parent, comprise the acquisitions of parent selection, parent fish rearing, induction gonad development and fertilized egg, concrete steps are as follows:
1. parent is selected
The bodily form is complete, and color and luster is normal, healthy, energetic, and ingest good, milter is more than 5 ages, and body length is more than 120 centimetres, and body weight is at more than 1600g; Raun more than 6 ages, female fish length more than 140 centimetres, body weight more than 2000 grams;
2. parent fish rearing
2.1 density domination
Density domination is at 2 ~ 3 tails/m
2, female-male proportion is 1:1.
2.2 fortification
Accelerating phase bait, based on oyster meat, yellow croaker and scomberomorus niphonius fish block, is thrown something and fed 2 times every day, and it is 2% ~ 3% of fish body weight that day throws the amount of raising; Egg-laying period bait is based on clam worm and the little yellow croaker block of living, and yellow croaker block adds the lecithin of B B-complex and 1 ~ 2 ‰ of 3 ~ 5 ‰ to promote parent gonad development before throwing something and feeding;
2.3 temperature control
When natural temperature is down to 4 ~ 5 DEG C, constant temperature 7 ~ 10 days, then rises 0.5 DEG C in every 3 days, and after temperature rises to 10 DEG C, constant temperature is cultivated;
2.4 illumination control
Intensity of illumination is 600 ~ 800Lx, and light application time changes with the change of water temperature, and when water temperature is down to 4 ~ 5 DEG C, light application time is 12h, keeps 7 ~ 10 days, then within every 3 days, extends 20min, and when illumination extends to 14h, permanent light is cultivated;
2.5 things concealeds are selected
Place in parents culture pond PVC plastic pipe as wolf eel things concealed, lay eggs and protect ovum place;
Further, the quantity of described PVC plastic pipe is identical with female parent stock, and pipe surrounding stone is fixed, with anti-rolling.
Further, the internal diameter of described PVC plastic pipe is 110mm, and length is 30 ~ 40cm.
2.6 daily management
Change water 300% ~ 400% every day, continue blowing aeration, keep dissolved oxygen at more than 5mg/L;
3. induce gonad development
Check wolf eel raun gonad development situation, hasten parturition to the raun of gonad development to IV phase, milter can extrude milky seminal fluid, does not need to hasten parturition.
Further, described wolf eel raun induced spawning method adopts intramuscular injection hormone method, adopt 80 ~ 100ppm eugenol anesthesia, 5 ~ 10min before injection hormone, after its loss of muscle tone, injection Luteinizing hormone releasing-hormone A2 (being called for short LHRH-A2) 10 ~ 20 μ g/kg hasten parturition.
Further, described wolf eel raun induced spawning method is that the LHRH-A2 adding 20 ~ 30 μ g/kg in female fish meal hastens parturition.
4. fertilized egg obtains
Aftereffect of the hastening parturition time is 42 ~ 50 hours, and the mode namely by natural spawning obtains fertilized egg.Wolf eel fertilized egg is viscid egg, and the fertilized egg after output is glued one, work and is attached on pvc pipe.In addition wolf eel also has and protects ovum behavior, and parent circle becomes a circle, fertilized egg is trapped among centre and hatches.
The present invention's beneficial effect compared with prior art:
The invention provides a kind of wolf eel genital regulating technology, after being regulated and controled by technical scheme of the present invention, wolf eel raun can carry out mating breeding 6 ages, milter in 5 ages, than parent under natural conditions until 7 ages could mating breeding time be advanced by 1 year respectively and 2 years; Solve wolf eel male and female and grow asynchronous technical barrier, reach synchronous spawning breeding; By throw in PVC plastic pipe meet a pair male and female live together a place, live in caves, protect the reproductive habits such as ovum, parent gonad development is cultivated success rate and is reached 85%, fertilization rate 80%.
Embodiment
Below by embodiment, technical scheme of the present invention is further explained, but protection scope of the present invention is not by any pro forma restriction of embodiment.
Embodiment 1
In December, 2012 we in development zone, Yantai, a plant has carried out genital regulating work to wolf eel parent, successfully regulation and control and induction parent gonadal maturation obtain high quality fertilized egg of scophthatmus, concrete steps are as follows:
1. parent is selected
Select wolf eel raun, each 50 tails of milter, selection criteria be that the bodily form is complete, color and luster normal, healthy, energetic, ingest well.Wolf eel parent is until 7 ages could breed in mating under field conditions (factors), and the sexual maturity time is more late.Therefore, under cultivating condition, milter should more than 5 ages, and body length is more than 120 centimetres, and body weight is at more than 1600g; Raun more than 6 ages, female fish length more than 140 centimetres, body weight more than 2000 grams.
2. parent fish rearing
2.1 density domination
Control at 2 ~ 3 tails/m
2, because wolf eel has monogamy, female-male proportion is 1:1.
2.2 fortification
Accelerating phase bait, based on oyster meat, yellow croaker and scomberomorus niphonius fish block, is thrown something and fed 2 times every day, and it is 2% ~ 3% of fish body weight that day throws the amount of raising.Egg-laying period bait is based on clam worm and the little yellow croaker block of living, and yellow croaker block adds the lecithin of B B-complex and 1 ~ 2 ‰ of 3 ~ 5 ‰ before throwing something and feeding, to promote parent gonad development.
2.3 temperature control
When natural temperature is down to 4 ~ 5 DEG C, keeps constant temperature 7 ~ 10 days, so often within latter 3 days, rise 0.5 DEG C, after temperature rises to 10 DEG C, constant temperature is cultivated.
2.4 illumination control
Wolf eel belongs to the type fish that lay eggs spring, the process that needs illumination of finally reaching maturity of parent extends gradually.After starting regulation and control, parents culture pond surrounding gobo blocks, and evenly install the incandescent lamp bulb 4 of 100w in dried up face 0.4 ~ 0.6m eminence, intensity of illumination is 600 ~ 800Lx.Regulation and control light application time changes with the change of water temperature.When water temperature is down to 4 ~ 5 DEG C, light application time is 6:00 ~ 18:00 12h altogether, keeps 7 ~ 10 days, within later every 3 days, extends 20min backward, and keep light application time at 6:00 ~ 22:00 14h altogether, permanent light is cultivated.
2.5 things concealeds are selected
Wolf eel has a pair male and female and to live together the characteristic that a place lives in caves, and in cultivation pond, lay the internal diameter identical with female parent stock is 110mm, length be the PVC plastic pipe of 30 ~ 40cm as wolf eel things concealed, lay eggs and protect ovum place.Pipe surrounding stone is fixed, with anti-rolling.
2.6 daily management
Change water 300% ~ 400% every day, continue blowing aeration, keep dissolved oxygen at more than 5mg/L; Every day 1 time, clear pond, to remove residual bait and ight soil, not timing measure water temperature, prevent fluctuating temperature amplitude excessive.Make regular check on parent gonad development situation, comprehensively analyze regulating effect.
3. induce gonad development
Production practices find, wolf eel is difficult to be issued to final Maturation and spawning at artificial regulatory method.After the genital regulating of 3 months, wolf eel parent sexual gland is obviously grown, and checks raun gonad development situation, hastens parturition to the female parent population of gonad development to IV phase.Because wolf eel is more violent, adopt 80ppm eugenol anesthesia 10min before injection hormone, after its loss of muscle tone, LH injection RH-A2 10 μ g/kg hastens parturition; Or the LHRH-A2 adding 20 ~ 30 μ g/kg in female fish meal hastens parturition.Milter can extrude milky seminal fluid under artificial regulatory, does not generally carry out hormone induction.
Aftereffect of the hastening parturition time is 50 hours, wolf eel parent natural spawning, ejaculation, obtains fertilized egg.Wolf eel fertilized egg is viscid egg, and the fertilized egg after output is glued one, work and is attached on pvc pipe.In addition wolf eel also has and protects ovum behavior, and parent circle becomes a circle, fertilized egg is trapped among centre and hatches.
Eventually pass through statistics wolf eel parent gonad development cultivation success rate and reach 85%, fertilization rate reached 80%, the incubation rate of fertilized egg under artificial incubation condition reaches 90%, and seed rearing survival rate is to 55%.
Claims (5)
1. a genital regulating method for wolf eel parent, comprises the acquisitions of parent selection, parent fish rearing, induction gonad development and fertilized egg, it is characterized in that concrete steps are as follows:
1) parent is selected
The bodily form is complete, and color and luster is normal, healthy, energetic, and ingest good, milter is more than 5 ages, and body length is more than 120 centimetres, and body weight is at more than 1600g; Raun more than 6 ages, female fish length more than 140 centimetres, body weight more than 2000 grams;
2) parent fish rearing
2.1 density domination
Density domination is at 2 ~ 3 tails/m
2, female-male proportion is 1:1;
2.2 fortification
Accelerating phase bait, based on oyster meat, yellow croaker and scomberomorus niphonius fish block, is thrown something and fed 2 times every day, and it is 2% ~ 3% of fish body weight that day throws the amount of raising; Egg-laying period bait is based on clam worm and the little yellow croaker block of living, and yellow croaker block adds the lecithin of B B-complex and 1 ~ 2 ‰ of 3 ~ 5 ‰ to promote parent gonad development before throwing something and feeding;
2.3 temperature control
When natural temperature is down to 4 ~ 5 DEG C, constant temperature 7 ~ 10 days, then rises 0.5 DEG C in every 3 days, and after temperature rises to 10 DEG C, constant temperature is cultivated;
2.4 illumination control
Intensity of illumination is 600 ~ 800Lx, and light application time changes with the change of water temperature, and when water temperature is down to 4 ~ 5 DEG C, light application time is 12h, keeps 7 ~ 10 days, then within every 3 days, extends 20min, and when illumination extends to 14h, permanent light is cultivated;
2.5 things concealeds are selected
Place in parents culture pond PVC plastic pipe as wolf eel things concealed, lay eggs and protect ovum place;
2.6 daily management
Change water 300% ~ 400% every day, continue blowing aeration, keep dissolved oxygen at more than 5mg/L;
3) gonad development is induced
Check wolf eel raun gonad development situation, hasten parturition to the raun of gonad development to IV phase, milter can extrude milky seminal fluid, does not need to hasten parturition;
4) fertilized egg obtains
Aftereffect of the hastening parturition time is 42 ~ 50 hours, and the mode namely by natural spawning obtains fertilized egg.
2. the genital regulating method of a kind of wolf eel parent according to claim 1, it is characterized in that the quantity of described PVC plastic pipe is identical with female parent stock, pipe surrounding stone is fixed, with anti-rolling.
3. the genital regulating method of a kind of wolf eel parent according to claim 1, it is characterized in that the internal diameter of described PVC plastic pipe is 110mm, length is 30 ~ 40cm.
4. the genital regulating method of a kind of wolf eel parent according to claim 1, it is characterized in that described wolf eel raun induced spawning method adopts intramuscular injection hormone method, 80 ~ 100ppm eugenol anesthesia, 5 ~ 10min is adopted before injection hormone, after its loss of muscle tone, injection Luteinizing hormone releasing-hormone A2 10 ~ 20 μ g/kg hastens parturition.
5. the genital regulating method of a kind of wolf eel parent according to claim 1, is characterized in that described wolf eel raun induced spawning method is that the Luteinizing hormone releasing-hormone A2 adding 20 ~ 30 μ g/kg in female fish meal is hastened parturition.
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| CN107810887A (en) * | 2017-11-03 | 2018-03-20 | 浙江海洋大学 | A kind of hickie cuttlefish forced ripening method and proprietary reagent |
| CN107926780A (en) * | 2017-11-17 | 2018-04-20 | 上海市水产研究所 | A kind of spot Ji embryonated egg preparation methods |
| CN110199922A (en) * | 2019-07-05 | 2019-09-06 | 深圳华大海洋科技有限公司 | A method of silver eel being changed by yellow eel for induction Female Japanese eel |
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| DE102012006275B3 (en) * | 2012-03-29 | 2013-01-31 | Wolfgang Markus | Breeding eels from eggs in a container filled water in controlled environment, comprises producing leptocephalus from fertilized eggs and rearing the leptocephalus in which the fertilized eggs in the container together bring picoplankton |
| CN102870713A (en) * | 2012-10-18 | 2013-01-16 | 山东省海洋水产研究所 | Sebastodes fuscescens parent fish warming cultivation and childbearing method |
| CN103004666A (en) * | 2012-12-28 | 2013-04-03 | 珠海市斗门区河口渔业研究所 | Breeding method for intermediate breeding of Anguilla marmorata fries |
| CN103125413A (en) * | 2013-02-28 | 2013-06-05 | 中国科学院海洋研究所 | Artificial maturing method for exopalaemon carinicauda in anestrous seasons |
| CN103125416A (en) * | 2013-03-25 | 2013-06-05 | 江苏中洋集团股份有限公司 | Ecological factorial breeding method of coilia ectenes parent fish |
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