CN103408351A - Polysaccharide composition and preparation method thereof - Google Patents
Polysaccharide composition and preparation method thereof Download PDFInfo
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- CN103408351A CN103408351A CN201310285412XA CN201310285412A CN103408351A CN 103408351 A CN103408351 A CN 103408351A CN 201310285412X A CN201310285412X A CN 201310285412XA CN 201310285412 A CN201310285412 A CN 201310285412A CN 103408351 A CN103408351 A CN 103408351A
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Abstract
The invention discloses a polysaccharide composition and a preparation method thereof, wherein the composition mainly comprises the following components in percentage by mass: 0.5-15 parts of sodium selenite; 5-50 parts of xylo-oligosaccharide; 1-30 parts of chitosan; 5-50 parts of trehalose; 2-60 parts of organic acid and 50-300 parts of water; the primary product of the composition is a dark red viscous liquid, the pH value is 4-6, and no precipitate is generated after the composition is diluted by water. The preparation method adopts the composition of the invention in percentage by mass and the following processes: dissolving sodium selenite in water at normal temperature and pressure, dissolving chitosan and organic acid in water, adding sodium selenite water solution into chitosan solution, stirring for 8 hr, and standing for 24 hr to obtain solution A; dissolving xylo-oligosaccharide and trehalose in water to obtain solution B; and then uniformly mixing the solution A and the solution B, and standing for 24 hours to obtain the polysaccharide composition.
Description
Technical field
The present invention relates to the agricultural fertilizer technology, be specially a kind of polysaccharide composition and preparation method thereof.Said composition is characterised in that several kinds of mineral elements such as being rich in xylo-oligosaccharide, chitosan, trehalose and nanometer selenium.
Background technology
Promoting the use of of chemical pesticide and chemical fertilizer, played huge promoter action to agriculture production, especially to the prevention and control of plant diseases, pest control of farm crop, improve the aspect such as crop yield per unit.But, a large amount of life-time service of chemical pesticide and inorganic chemical fertilizer, its negative impact is also more and more significant.Cause harmful organism to develop immunity to drugs on the one hand, along with consumption is increasing, formed vicious cycle, caused environmental pollution; On the other hand, excessively use fertilizer and pesticide, the Agro-ecology balance is done great damage, make soil deterioration, agricultural byproducts and livestock product pesticide residue severe overweight, nutritive element is unbalance, and interior quality descends, and the mankind healthy brought to serious threat.The mankind every day need to be from taking in various nutritive elements to keep fit and normal physiological function meals.The intake deficiency of various nutritive elements in human body, will cause function of human body to descend even ill, supplement corresponding nutritive element and can improve the health level of human body, and then eliminate associated conditions.Therefore, Development of Novel fertilizer, agricultural chemicals, substitute traditional chemical pesticide and chemical fertilizer, enriches the crop alimentary element, become the inexorable trend of modern agricultural development.
Selenium is a kind of important trace element of needed by human, and human body lacks selenium can cause many diseases, and wherein cancer, Keshan disease are just relevant with scarce selenium.And China is the country that lacks selenium, the region area 70% or more is to belong to the big and medium-sized cities such as scarce selenium, few selenium area, particularly North China, northeast and northwest all to belong to and lack the selenium area, in food, does not reach the above standard benefit selenium level of everyone 40 micrograms every day.Therefore, many benefit selenium products and selenium-enriched fertilizer are constantly released.But, only with regard to current agricultural selenium-enriched fertilizer, be mostly simple with regard to mending selenium, to mend selenium, can not meet disease control, soil remediation and the multiple needs such as degeneration-resistant of crop.
Summary of the invention
For the deficiencies in the prior art, the technical problem that quasi-solution of the present invention is determined is that a kind of polysaccharide composition and preparation method thereof is provided.Said composition is rich in the several kinds of mineral elements such as xylo-oligosaccharide, chitosan, trehalose and nanometer selenium, bestow farm crop, not only can significantly improve the selenium content of crop and fruit thereof, and can prevent and treat the disease that crop is caused by mushroom, improvement soil, promote growth, improves resistance and the drought-resistant ability of crop, increase output, improve quality; This preparation method adopts normal temperature and pressure production, and technique is simple, easy to operate, is applicable to suitability for industrialized production.
The technical scheme that the present invention solves described composition technical problem is, designs a kind of polysaccharide composition, and the massfraction that it is characterized in that said composition forms and is mainly: 0.5～15 part of Sodium Selenite; 5～50 parts of xylo-oligosaccharides; 1～30 part of chitosan; 5～50 parts of trehaloses; 50～300 parts, organic acid 2-60 part and water; Wherein, the purity of xylo-oligosaccharide >=95%; The deacetylation of chitosan >=85%; Organic acid is the combination of one or more arbitrary proportions in acetic acid, citric acid or xitix; The first batch of newly-designed products of said composition is the scarlet thick liquid, and pH is 4～6, nothing precipitation after dilute with water.
The technical scheme that the present invention solves described preparation method's technical problem is, design a kind of preparation method of polysaccharide composition, this preparation method adopts the massfraction of polysaccharide composition of the present invention to form and following technique: at normal temperatures and pressures, (1), first Sodium Selenite is dissolved in water, just chitosan and organic acid are dissolved in water again, after all dissolving, sodium selenite aqueous solution is added in chitosan and aqueous solutions of organic acids, and constantly stirred 8 hours, the pH value of maintenance solution≤5, standing 24 hours, make A solution; (2), xylo-oligosaccharide and trehalose are dissolved in water together, after all dissolving, make B solution; (3), A solution is mixed and stirs with B solution, standing 24 hours, namely make described polysaccharide composition.
Compared with prior art, preparation method's normal temperature and pressure of the present invention is produced, and technique is simple, easy to operate, pollution-free, and less energy-consumption is easy to promote, and is applicable to suitability for industrialized production.Polysaccharide composition result of use of the present invention is obvious, not only when making crop and fruit thereof be rich in organic selenium and other trace elements, realize that low toxicity is efficient, reduce the chemical pesticide consumption, alleviate environmental pressure, and can strengthen anti-adversity and the diseases and insect pests resistance of plant, photosynthesis strengthens, for the high crop yield stable yields has been established important foundation, simultaneously can also improve soil, reduce soil compaction.It is easy to use various that the polysaccharide composition of nanometer selenium is rich in the present invention, both can be used as foliage-spray after dilution, also can root executes to chase after and water; The stoste use of can dressing seed, can sneak into organic base manure and use together, also can adopt after conventional drying to make pulvis and use.
Embodiment
Below in conjunction with embodiment, further narrate the present invention.
The polysaccharide composition (abbreviation composition) of the present invention's design, is characterized in that the massfraction composition of said composition is mainly: 0.5～15 part of Sodium Selenite; 5～50 parts of xylo-oligosaccharides; 1～30 part of chitosan; 5～50 parts of trehaloses; 50～300 parts, organic acid 2-60 part and water.
For further enriching the nutritive element of farm crop, increase kind and the content of crop trace element, improve the quality of crop, the demand that adapts to extraordinary plant husbandry, the further feature of the present composition is: said composition also comprises at least a in the following substance that massfraction forms: 0.5～3 part of 1～20 part of element Ge, 1～20 part of zinc element, 1～5 part of manganese element, 1～5 part of boron or molybdenum element.
The massfraction preferred group of the present composition becomes: 1～15 part of Sodium Selenite; 5～50 parts of xylo-oligosaccharides; 1～30 part of chitosan; 5～50 parts of trehaloses; Organic acid 2-60 part; 1～20 part of germanium dioxide; 1～20 part of zinc acetate; 1～10 part of manganous acetate, 1～10 part of boric acid; 50～200 parts, 1～5 part of ammonium molybdate and water.
The purity of the described xylo-oligosaccharide of the present composition >=95%;
The deacetylation of chitosan >=85%;
Organic acid can be the combination of one or more arbitrary proportions of acetic acid, citric acid or xitix;
Element Ge derives from germanium dioxide or organic germanium;
Zinc element derives from zinc acetate or chelated zinc;
Manganese element derives from manganous acetate or Chelated Manganese;
Boron derives from boric acid;
Molybdenum element derives from ammonium molybdate.
This composition is red thick liquid, and pH is 4～6, nothing precipitation after dilute with water.After bestowing farm crop, not only can improve the selenium and other trace elements content of crop, but also can prevent and treat Micobial Disease, improve photosynthesis, improve degeneration-resistant, the drought-resistant ability of plant; Bestow in soil and can improve soil, increase fertility of land; Can be mixed with acid agrichemical while for the page, spraying, and can improve drug effect.
Xylo-oligosaccharide claims again wood oligose, be by 2～7 wood sugars with β-Isosorbide-5-Nitrae glycosidic link oligose that is formed by connecting, its main component is xylo-bioses and xylotriose.Typical xylo-oligosaccharide raw materials for production have hardwood, corn cob, straw, bagasse, rice hulls, beer saccharification grain and wheat bran.As new resource food, after the people is edible, can optionally promote the proliferation activity of intestinal bifidobacteria and milk-acid bacteria, suppress the harmful bacteria growth, improve the enteric microorganism environmental functional, its bifidobacterium factor functional is 10-20 times of other polymerization carbohydrate.Agriculturally, xylo-oligosaccharide can excite plant to produce the disease resistance materials such as phenols, plant protecting chemical, improves the activity of relevant disease-resistant enzyme; As biological pesticide, xylo-oligosaccharide good water solubility, toxicological harmless, free from environmental pollution, life-time service do not cause that plant produces the advantages such as special disease resistance, using dosage are low; As its crop of nutritive substance cultivation agriculture, it can regulate and control crop growth, improve the output of fruit, simultaneously by affect the absorption of soil microorganisms flora raising farm crop to elements such as nitrogen potassium.
Trehalose is by the nonreducing sugar of two glucose molecules with 1,1-glycosidic link formation, is a kind of safe and reliable natural glucide, and its most peculiar function is its biological characteristics.In many organisms, trehalose not only exists with the free sugar form, but also as the integral part of various glycolipids.Recent research shows, many species, under poor environment (as arid, high temperature, freezing etc.) condition, can be resisted external influence by trehalose synthesis in control agent.Trehalose is the material that a kind of stress situation that environmental change is formed has high resistance, is also a kind of typical stress metabolite in organism.Under various severe environment, also still can be to the good provide protection of the biomacromolecules such as microbial film, protein and nucleic acid performance of species.Up-to-date research shows, ectogenic trehalose has the good nonspecific provide protection to organism and biomacromolecule equally.Due to the profound meaning of trehalose to the life protection, therefore there is the people trehalose to be called to " sugar of life ".The biological characteristics of this excellence of trehalose just, after making the present composition bestow farm crop, make farm crop have the excellent anti-adversity such as drought-resistant, low temperature resistant, high temperature resistant.
Chitosan, be the product of the de-N-ethanoyl of chitin, and chemical name is Chitosan (1-4)-2-amino-B-D glucose, is the crust that is mainly derived from Crustacean.The premium propertiess such as the biological functionality of this natural polymer of chitosan and consistency, blood compatibility, security, microbic resolvability are by all trades and professions extensive concern, have obtained major progress in the applied research of the numerous areas such as medicine, food, chemical industry, makeup, water treatment, METAL EXTRACTION and recovery, biochemistry and biomedical engineering.Chitosan, in agricultural production application, can not only make crop growing state good, robust plant, and photosynthesis strengthens, and aspect resistance, disease and insect resistance, is also showing positive effect, has and stablizes and significant the effect of increasing income.In addition, chitosan also improves larger meaning is arranged the improvement of crop quality and food value and economic worth.On the other hand, chitosan can be used as plant-growth regulator, promotes crop yield to improve, and also can be used as soil improvement agent and be used to gathering in the crops the fresh-keeping etc. of rear agricultural-food, and especially it is at the focus that suppresses aspect Plant diseases becoming gradually people's research.Large quantity research shows, chitosan can inducing plant produce wide spectrum resistance, strengthen plant the self-defence ability, suppress the growth of various plants pathogenic micro-organism, and it is nontoxic and can be microbiological deterioration, can not produce pollution to environment.This likely plays an important role it in agriculture production as a kind of novel environment friendly agricultural.The more important thing is, chitosan is the high molecular polymer with positive charge, has stronger reducing power, the also this reducing power of chitosan just, the plasma selenium that makes Sodium Selenite in this composition after chitosan absorption, parcel, chelating and progressively reduction generates the simple substance nanometer selenium or/and selenium chitosan, and the selenium element can fully be disperseed in composition.Chitosan also has good film forming properties, can make the beneficiating ingredient of the present composition be adsorbed on preferably blade face, branches and tendrils, cane, the seed-coat of plant, for plant, absorbs, and has slow releasing function.
Selenium is a kind of trace element of needed by human, participates in the metabolism body endoperoxide and iodine, regulates the level of iodine in organism and the level of free radical, but enhancing body diseases prevention, resistance against diseases.Though mend selenium, can effectively prevent and treat various diseases, between the nutrition dosage of inorganic selenium and toxicity dose, scope is narrow, to such an extent as to people are studying the efficient selenium product of low toxicity for many years always.Up-to-date research shows, organoselenium absorbs the aspects such as efficiency, hypotoxicity and all is better than inorganic selenium at height, and red nanometer selenium more is better than organoselenium in many aspects, demonstrates the efficient feature of low toxicity.Research shows, per os medium lethal dose (LD_50) organoselenium, selenium chitosan, the nanometer selenium of small white mouse improved to 2.3 times, 6.3 times and 7.5 times than inorganic selenium compound respectively.In the agricultural application field, nanometer selenium, selenium chitosan have efficient absorption utility and good biological organic transformation performance equally, plant will change in the organoselenium form bodies such as selenium-L-glutamic acid, selenocystein, selenium-methyl selenium-halfcystine after absorbing nano-selenium element, become the plant of being rich in organoselenium; With respect to traditional use inorganic selenium mode, nanometer selenium both can greatly reduce the consumption of selenium element, reduced costs, and can alleviate the harm that the inorganic selenium element causes environment such as surface water, underground water and soils again; For remaining in crop and fruit surface thereof and the inner not yet inorganic selenium of organic transformation, the hypotoxicity of nanometer selenium, selenium chitosan has more very important safe meaning.
Element Ge, although be at present not yet clearly the necessary trace element of human body, a kind of human body beneficial element really, many glossy ganodermas with special healthy functions, ginseng etc., its special healthy functions it is believed that relevant with the content of its germanium.Research shows, the almost non-toxic property of organic germanium, has the self-interference of bringing out element, increase the nk cytoactive, the activation phagocytic cell, promote body to produce antitumor, anti-ageing biological action, as a kind of important nutritive element, former health ministry has been listed organic germanium in the new resource food list of formal production, and has been widely used in the various fields such as beverage, food and health care.Therefore, use contains the extraordinary fertilizer of element Ge, make farm crop and fruit thereof contain element Ge and by the biological process of plant, realize the organic transformation of element Ge, and then, for people provide new wholesome food, will be one of effective selection of new special agricultural.
Zinc is composition or the coenzyme of the large enzyme of human body six, 200 kinds of metalloenzyme, and general metabolism is played to wide application, by people, is described as " flowers of life ".Simultaneously, zinc element is also necessity trace element of plant-growth, therefore, uses the fertilizer contain zinc element, improves the zinc content of farm crop and fruit thereof, not only can Promoting plant growth, and people's health is had important practical significance.
What is more important, aspect biological activity, selenium and germanium have synergy, and selenium element and zinc element also have synergy.The present composition organically combines red nano selenium, organic germanium and organic zinc, make farm crop and fruit thereof can increase simultaneously the content of selenium, germanium, zinc, after making people edible, can supplement simultaneously these three kinds of trace elements, and can effectively bring into play three's synergistic effect, for the mankind's health contributes.
The present invention has designed the preparation method of described composition simultaneously, this preparation method adopts the massfraction of nanometer selenium polysaccharide composition of the present invention to form and following technique: at normal temperatures and pressures, (1), first Sodium Selenite is dissolved in water, just chitosan and organic acid are dissolved in water again, after all dissolving, sodium selenite aqueous solution is added in chitosan solution, and constantly stirred 8 hours, the pH value of maintenance solution≤5, then standing 24 hours, make A solution; (2), xylo-oligosaccharide and trehalose are dissolved in water together, after all dissolving, make B solution; (3), A solution, B solution are mixed, and stir, standing 24 hours, namely make described polysaccharide composition.For accelerating the nanometer process of selenium element, can add the part xitix in B liquid.
The present composition increases or adjusts when new component is arranged, and its preparation method is done corresponding adjustment.
The present composition is according to design, also can comprise at least a in the following substance that massfraction forms: 1～5 part of 1～20 part of element Ge, zinc element 1～20,1～10 part of manganese element, 1～10 part of boron or molybdenum element.In preparation B solution process, described substance is dissolved in water and gets final product together with xylo-oligosaccharide and trehalose.But when the substance element Ge is germanium dioxide, must first germanium dioxide be dissolved in potassium hydroxide solution, then use after with organic acid, the pH value of this solution being adjusted to 5.5 left and right.
The present composition is when designing and contain described substance simultaneously: when 0.5～3 part of 1～20 part of element Ge, zinc element 1～20,1～5 part of manganese element, 1～5 part of boron or molybdenum element, its preparation method as previously mentioned, is dissolved in water together with xylo-oligosaccharide and trehalose when preparation B solution.
Compared with prior art, preparation method of the present invention is normal temperature and pressure production, and technique is simple, easy to operate, pollution-free, and less energy-consumption is easy to promote, and is applicable to suitability for industrialized production.
The present composition is the scarlet thick liquid, and the pH value is 5～5.5.This is a kind of basic product, according to different purposes, utilizes this basic product, can prepare different secondary products.
The present invention does not address part and is applicable to prior art.
Below provide specific embodiments of the invention.These embodiment, only for further concrete narration the present invention, do not limit the protection domain of the application's claim.
Embodiment 1
Design a kind of polysaccharide composition, its material by following massfraction forms: 0.5 part of Sodium Selenite; 2 parts of chitosans; 5 parts of xylo-oligosaccharides; 5 parts of trehaloses; 1 part of organic germanium (Ge-132); 1 part of zinc acetate; 1 part of manganous acetate; 1 part of boric acid; 0.5 part of ammonium molybdate; 4 parts of citric acids; 50 parts, 1 part, xitix and water.Its preparation method is:
1, under normal temperature and pressure, first Sodium Selenite is added to 2 parts of dissolvings of water, more just chitosan and citric acid add 30 parts of dissolvings of water, after all dissolving, sodium selenite aqueous solution is added in chitosan solution, and constantly stirred 8 hours, and the pH value of maintenance solution≤5, then standing 24 hours, make A solution;
2, xylo-oligosaccharide, trehalose, organic germanium, manganous acetate, zinc acetate, boric acid, ammonium molybdate and xitix are added together to 18 parts of water dissolution, after all dissolving, make B solution;
3, A solution is mixed with B solution, stir, standing 24 hours, namely make described polysaccharide composition.
Embodiment 2
Design a kind of polysaccharide composition, its material by following massfraction forms: 5 parts of Sodium Selenites; 10 parts of chitosans; 30 parts of xylo-oligosaccharides; 30 parts of trehaloses; 15 parts of germanium dioxides; 28 parts, potassium hydroxide; 10 parts of zinc acetates; 5 parts of manganous acetates; 5 parts of boric acid; 35 parts of citric acids; 200 parts, 5 parts, xitix and water.Its preparation method is:
1, under normal temperature and pressure, first Sodium Selenite is added to 10 parts of dissolvings of water, more just chitosan and 20 parts of citric acids add 90 parts of dissolvings of water, after all dissolving, sodium selenite aqueous solution is added to chitosan solution, and constantly stirred 8 hours, and the pH value of maintenance solution≤5, then standing 24 hours, make A solution;
2, potassium hydroxide is dissolved in to 100 parts of water, then after adding germanium dioxide to be stirred to whole dissolvings, add again xylo-oligosaccharide, trehalose, zinc acetate, manganous acetate, boric acid after adding 15 parts of citric acids, 5 parts of dissolution of ascorbic acid, after all dissolving, make B solution;
3, A liquid, B liquid mixed and stirs, standing 24 hours, namely making described polysaccharide composition.
Embodiment 3
Design a kind of polysaccharide composition, its material by following massfraction forms: 10 parts of Sodium Selenites; 20 parts of chitosans; 15 parts of xylo-oligosaccharides; 15 parts of trehaloses; 20 parts of organic germaniums; 20 parts of zinc acetates; 35 parts of acetic acid; 10 parts, xitix; 200 parts, water.Its preparation method is:
1, under normal temperature and pressure, first Sodium Selenite is added to 30 parts of dissolvings of water, more just chitosan and acetic acid add 100 parts of dissolvings of water, after all dissolving, sodium selenite aqueous solution added to chitosan solution and constantly stirred 8 hours, and the pH value of maintenance solution≤5, then standing 24 hours, make A solution;
2, xylo-oligosaccharide, trehalose, organic germanium, zinc acetate and xitix are added together to 70 parts of water dissolution, after all dissolving, make B solution;
3, A liquid, B liquid mixed and stirs, standing 24 hours, namely making described polysaccharide composition.
Embodiment 4
Design a kind of polysaccharide composition, its material by following massfraction forms: 15 parts of Sodium Selenites; 30 parts of chitosans; 50 parts of xylo-oligosaccharides; 50 parts of trehaloses; 50 parts of acetic acid; 10 parts, xitix; 300 parts, water.Its preparation method is:
1, under normal temperature and pressure, first Sodium Selenite is added to 40 parts of dissolvings of water, more just chitosan and acetic acid add 200 parts of dissolvings of water, after all dissolving, sodium selenite aqueous solution added to chitosan solution and constantly stirred 8 hours, and the pH value of maintenance solution≤5, then standing 24 hours, make A solution;
2, xylo-oligosaccharide, trehalose and xitix are added together to 60 parts of water dissolution, after all dissolving, make B solution;
3, A liquid, B liquid mixed and stirs, standing 24 hours, namely making described polysaccharide composition.
Embodiment 5
Design a kind of polysaccharide composition, its material by following massfraction forms: 1 part of Sodium Selenite; 5 parts of chitosans; 25 parts of xylo-oligosaccharides; 25 parts of trehaloses; 2 parts of organic germaniums (Ge-132); 2 parts of zinc acetates; 1 part of manganous acetate; 1 part of boric acid; 1 part of ammonium molybdate; 10 parts of citric acids; 100 parts, water.Its preparation method is:
1, under normal temperature and pressure, first Sodium Selenite is added to 5 parts of dissolvings of water, more just chitosan and citric acid add 50 parts of dissolvings of water, after all dissolving, sodium selenite aqueous solution added to chitosan solution and constantly stirred 8 hours, and the pH value of maintenance solution≤5, then standing 24 hours, make A solution;
2, xylo-oligosaccharide, trehalose, organic germanium, manganous acetate, zinc acetate, boric acid and ammonium molybdate are added together to 45 parts of water dissolution, after all dissolving, make B solution;
3, A liquid, B liquid mixed and stirs, standing 24 hours, namely making described polysaccharide composition.
Claims (5)
1. a polysaccharide composition, is characterized in that the massfraction composition of said composition is mainly: 0.5～15 part of Sodium Selenite; 5～50 parts of xylo-oligosaccharides; 1～30 part of chitosan; 5～50 parts of trehaloses; 50～300 parts, organic acid 2-60 part and water; Wherein, the purity of xylo-oligosaccharide >=95%; The deacetylation of chitosan >=85%; Organic acid is the combination of one or more arbitrary proportions in acetic acid, citric acid or xitix; The first batch of newly-designed products of said composition is the scarlet thick liquid, and pH is 4～6, nothing precipitation after dilute with water.
2. polysaccharide composition according to claim 1 is characterized in that at least a in following substance that said composition also comprises that massfraction forms: 0.5～3 part of 1～20 part of element Ge, 1～20 part of zinc element, 1～5 part of manganese element, 1～5 part of boron or molybdenum element; Wherein, zinc element derives from zinc acetate or chelated zinc; Manganese element derives from manganous acetate or Chelated Manganese; Magnesium elements derives from amino acid-magnesium chelate.
3. polysaccharide composition according to claim 2, is characterized in that the massfraction of said composition consists of: 1～15 part of Sodium Selenite; 5～50 parts of xylo-oligosaccharides; 1～30 part of chitosan; 5～50 parts of trehaloses; Organic acid 2-60 part; 1～20 part of germanium dioxide; 1～20 part of zinc acetate; 1～10 part of manganous acetate, 1～10 part of boric acid; 50～200 parts, 1～5 part of ammonium molybdate and water.
4. the preparation method of a polysaccharide composition, this preparation method adopts the massfraction of the described polysaccharide composition of claim 1 to form and following technique: at normal temperatures and pressures, (1), first Sodium Selenite is dissolved in water, just chitosan and organic acid are dissolved in water again, after all dissolving, sodium selenite aqueous solution are added in chitosan and aqueous solutions of organic acids, and constantly stirred 8 hours, the pH value of maintenance solution≤5, standing 24 hours, make A solution; (2), xylo-oligosaccharide and trehalose are dissolved in water together, after all dissolving, make B solution; (3), A solution is mixed and stirs with B solution, standing 24 hours, namely make described polysaccharide composition.
5. the preparation method of polysaccharide composition according to claim 4, while it is characterized in that containing the described substance of claim 2 in described polysaccharide composition, in preparation B solution process, be dissolved in water this substance together with xylo-oligosaccharide and trehalose; But when the substance element Ge is germanium dioxide, must first germanium dioxide be dissolved in potassium hydroxide solution, then use after with organic acid, the pH value of this solution being adjusted to 5.5 left and right.
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| CN112778048A (en) * | 2021-01-22 | 2021-05-11 | 北部湾大学 | Liquid selenium fertilizer rich in marine active ingredients and preparation method thereof |
| CN114230686A (en) * | 2021-12-22 | 2022-03-25 | 众合发(北京)生物科技发展有限公司 | Preparation process, device and application of selenized chitosan oligosaccharide |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010099264A (en) * | 2001-09-17 | 2001-11-09 | 강경철 | Process for Preparing Liquid Composite Fertilizer by Chitosan |
| CN1415310A (en) * | 2002-11-18 | 2003-05-07 | 安徽大学 | Nano selenium of chitin and its preparation method |
| CN1594229A (en) * | 2004-06-18 | 2005-03-16 | 株洲市湘东氨基酸有限公司 | Composite amino acid chitosan fertilizer |
| CN1594236A (en) * | 2004-07-06 | 2005-03-16 | 湖南大学 | Nano selenium nutrition conditioning agent for fruit and vegetable and its preparing process |
| CN1844062A (en) * | 2006-05-10 | 2006-10-11 | 沈平瑞 | Selenium-enriched fertilizer sprinkled on leaves and preparation method thereof |
| CN102267839A (en) * | 2011-05-19 | 2011-12-07 | 浙江天凌农业科技有限公司 | Plant organic nutrient solution containing saccharicterpenin and preparation method thereof |
| CN102584403A (en) * | 2012-03-02 | 2012-07-18 | 天津市星河系科技有限公司 | Ion nutritional liquid fertilizer and preparation method thereof |
| CN102701844A (en) * | 2012-05-21 | 2012-10-03 | 成钢 | Rich-selenium-germanium trace element nanometer nutrition fertilizer for vegetable and fruit soilless culture |
| CN102924180A (en) * | 2012-11-22 | 2013-02-13 | 海南正业中农高科股份有限公司 | Oligosaccharide-containing soilless culture liquid fertilizer |
-
2013
- 2013-07-09 CN CN201310285412.XA patent/CN103408351B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010099264A (en) * | 2001-09-17 | 2001-11-09 | 강경철 | Process for Preparing Liquid Composite Fertilizer by Chitosan |
| CN1415310A (en) * | 2002-11-18 | 2003-05-07 | 安徽大学 | Nano selenium of chitin and its preparation method |
| CN1594229A (en) * | 2004-06-18 | 2005-03-16 | 株洲市湘东氨基酸有限公司 | Composite amino acid chitosan fertilizer |
| CN1594236A (en) * | 2004-07-06 | 2005-03-16 | 湖南大学 | Nano selenium nutrition conditioning agent for fruit and vegetable and its preparing process |
| CN1844062A (en) * | 2006-05-10 | 2006-10-11 | 沈平瑞 | Selenium-enriched fertilizer sprinkled on leaves and preparation method thereof |
| CN102267839A (en) * | 2011-05-19 | 2011-12-07 | 浙江天凌农业科技有限公司 | Plant organic nutrient solution containing saccharicterpenin and preparation method thereof |
| CN102584403A (en) * | 2012-03-02 | 2012-07-18 | 天津市星河系科技有限公司 | Ion nutritional liquid fertilizer and preparation method thereof |
| CN102701844A (en) * | 2012-05-21 | 2012-10-03 | 成钢 | Rich-selenium-germanium trace element nanometer nutrition fertilizer for vegetable and fruit soilless culture |
| CN102924180A (en) * | 2012-11-22 | 2013-02-13 | 海南正业中农高科股份有限公司 | Oligosaccharide-containing soilless culture liquid fertilizer |
Non-Patent Citations (3)
| Title |
|---|
| 曾汉民: "《功能纤维》", 28 February 2005, 化学工业出版社 * |
| 聂继云: "《果品质量安全分析技术》", 31 December 2009, article "锗的测定", pages: 160-161 * |
| 陈国荣: "《果品质量安全分析技术》", 31 December 2009, 华东理工大学出版社 * |
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