CN105110921A - Microbial fertilizer for accelerating crop growth and preparation method for microbial fertilizer - Google Patents
Microbial fertilizer for accelerating crop growth and preparation method for microbial fertilizer Download PDFInfo
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Abstract
The invention discloses a microbial fertilizer for accelerating crop growth and a preparation method for the microbial fertilizer. The microbial fertilizer comprises the components in parts by mass: 100 parts of plant straws, 8-16 parts of rhamnolipid, 4-10 parts of nucleotide salt, 60-80 parts of chitosan hydrochloride, 0.8-1.2 parts of a bacillus subtilis fungicide, 6-10 parts of lactic acid bacteria powder, 4-8 parts of saccharomycetes powder, 8-20 parts of monopotassium phosphate, 6-10 parts of ferrous sulfate, 10-15 parts of calcium carbonate, 1-3 parts of magnesium chloride, 1-3 parts of ammonium molybdate, 1-3 parts of zinc sulfate, 1-3 parts of manganese sulfate and 1-3 parts of boric acid. The rhamnolipid and the nucleotide salt in the microbial fertilizer have mechanisms of promoting growth and play a synergistic role if being compounded in use, so that the growth promotion to the plant is remarkably improved; in addition, the rhamnolipid further can improve the degradation rate of plant straw fibers by bacillus subtilis with brevibacterium. In addition, the chitosan hydrochloride can degrade plant and animal residues and trace metal elements and convert those into nutrients of plants so as to promote health of the plants.
Description
Technical field
The present invention relates to the technical field of microbial fertilizer, particularly relate to a kind of microbial fertilizer accelerating crop growth and preparation method thereof.
Background technology
In China, for many years due to the life-time service of chemical fertilizer, fertilizer is not enough, all kinds of nutrients ratio imbalance, cause the destruction that farmland ecological environment, soil physico-chemical property and soil microflora are subject in various degree, have impact on the safety of agricultural-food to a certain extent.At present, crop prevention and elimination of disease and pests mainly uses chemicals and toxic pesticides through mix and blend, in being manured into soil or on farm crop, the agricultural chemicals that life-time service is a large amount of and chemical fertilizer, easily make insect produce resistance, soil compaction, pollute water source, soil and farm crop, agricultural product quality declines.And bio-feritlizer is a kind of environmental friendly fertilizer, become one of important directions of the future of agriculture Sustainable development.
In prior art, the microbial fertilizer of Promoting plant growth normally by adjusting contained microbiobacterial agent, the kind proportioning of nutritive element of inorganic fertilizer comes and strengthens fertilizer efficiency.Such as, Chinese patent CN104402594A discloses a kind of green microbial fertilizer of Promoting plant growth, comprises the material of following parts by weight: enzymatic microorganism 0.01-0.02 part, stone flour 23-30 part, peat composed of rotten mosses powder 60-70 part, bacillus thruingensis powder 0.01-0.02 part, bud pole bacterium powder 0.01-0.02 part, ammonium hydrogen phosphate 12-16 part, maize straw 60-70 part, soybean stalk 40-50 part, calcium monohydrogenphosphate 8-10 part, ground phosphate rock 1-8 part, male spiral case powder 20-30 part.This microbial fertilizer, owing to only relying on fertilizer efficiency to the promotion of the growth of plant, exists plant-growth raising speed slower like this.
Summary of the invention
In view of this, one aspect of the present invention provides a kind of microbial fertilizer accelerating crop growth quickly.
Accelerate a microbial fertilizer for crop growth, comprise 100 parts of straws, 8 ~ 16 parts of rhamnolipids, 4 ~ 10 parts of nucleosides hydrochlorates, 60 ~ 80 parts of chitosan hydrochlorides, 0.8 ~ 1.2 part of bacillus subtilis microbial agent, 6 ~ 10 parts of lactobacillus powders, 4 ~ 8 parts of yeast powder, 8 ~ 20 parts of potassium primary phosphates, 6 ~ 10 parts of ferrous sulfate, 10 ~ 15 parts of calcium carbonate, 1 ~ 3 part of magnesium chloride, 1 ~ 3 part of ammonium molybdate, 1 ~ 3 part of zinc sulfate, 1 ~ 3 part of manganous sulfate, 1 ~ 3 part of boric acid according to mass parts.
As a preferably embodiment, the microbial fertilizer of Promoting plant growth in the present invention, comprises 100 parts of straws, 10 parts of rhamnolipids, 6 parts of nucleosides hydrochlorates, 70 parts of chitosan hydrochlorides, 1 part of bacillus subtilis microbial agent, 8 parts of lactobacillus powders, 6 parts of yeast powder, 12 parts of potassium primary phosphates, 8.5 parts of ferrous sulfate, 13 parts of calcium carbonate, 2 parts of magnesium chlorides, 2 parts of ammonium molybdates, 2 parts of zinc sulfate, 2 parts of manganous sulfates, 1.5 parts of boric acid according to mass parts.
Aforementioned, rhamnolipid is, the bio-surfactant of a kind of biological metabolism character produced by pseudomonas or Burkholderia class.As the rhamnolipid in the present invention, itself there is activated plant somatomedin, improve blade permeability, extend the nutritive element attachment time, and by closing the huge legendary turtle of trace element, avoiding nutritive element and being transformed by soil absorption, drastically increasing the assimilated efficiency of plant to nutrient.Utilize plant minimum nutrient rate principle simultaneously, rationally with the addition of the necessary nutritive element of plant-growth, make its reasonable supplement plant desired nutritional while coordinate plant growth, thus healthy and strong plant, improve output.Another person, in the present invention, rhamnolipid also can to the heavy metal ion existed in soil, and such as chromium, copper, zinc, lead, cadmium etc. have stronger absorption or capacity of decomposition, play the purification to edatope.Moreover the rhamnolipid contained as this Institute of Micro-biology also can play synergy with subtilis, and strengthening enzyme is lived, and improves subtilis to the degradation rate of plant straw fibers.
Nucleosides hydrochlorate, the production that nucleosides hydrochlorate is particularly applied to traditional Chinese medicine of whole straw can improve its Yield and quality, quality, enhancing resistance, uniformity coefficient, reduce nuisance content, cost-saving, increase income, synergy.Here, nucleosides hydrochlorate preferably but be not limited in 5 '-adenylic acid (AMP) dipotassium or disodium, 5 '-bird adenylic acid (AMP) dipotassium or disodium, 5 '-cytidylic acid dipotassium or disodium, 5 '-uridylic acid dipotassium or disodium and any mixture.
Aforementioned, chitosan (chitosan) is also known as chitosan, be that the chitin (chitin) extensively existed by nature obtains through deacetylation, chemical name is Chitosan (1-4)-2-amino-B-D glucose.Chitosan hydrochloride refers to the salt that chitosan and hydrochloric acid are formed, and its No. CAS is 70694-72-3.Compared to chitosan, chitosan hydrochloride is easily water-soluble.The chitosan that in the present invention, chitosan hydrochloride produces can give plant insecticide, disease-resistant, plays the effect of fertilizer, plant and animal residues and micro-metals in soil can be decomposed again, thus be converted into the nutrient substance of plant, strengthen via plant immunity, promote the health of plant.In addition, chitosan itself is containing glucose unit, can also milk-acid bacteria contained by this microbial fertilizer and yeast use after in soil metabolism breeding important sugared source is provided.
Aforementioned, lactobacillus powder and yeast powder refer to containing the mixture of active bacteria (milk-acid bacteria or yeast) with its meta-bolites.The concrete kind of the present invention to lactobacillus powder and yeast powder does not limit, commercially available implemented the present invention.
Aforementioned, the general name of ripe farm crop cauline leaf (fringe) part of straw.Limit especially as stalk of the present invention, the example of rice straw, wheat stalk, maize straw, rape stalk, cotton stalk can be enumerated.
In the present invention, the multiple vitamin B group such as subtilis energy synthesise vitamins B1, B2, B6, nicotinic acid, improves the activity of animal body internal interference element and scavenger cell.Preferably, the living bacteria count concentration of bacillus subtilis microbial agent be 60 ~ 10,000,000,000/gram.
Aforementioned, subtilis also has following effect: 1. moisture retention is strong: the natural materials forming intensity very excellent gathers Vetsin, is the protective membrane of soil, prevents fertile part and moisture loss; 2. organic matter decomposition power is strong: while propagation, can disengage highly active amylase, and the macromolecular substance that difficulty is decomposed is resolved into available small-molecule substance; 3. produce abundant metabolism resultant: synthesize the materials such as multiple organic acid, enzyme, physiologically active, and other multiple nutrient be easily utilized; 4. antibacterial, harmful power of going out is strong: there is the advantage of taking up space, suppress the growth and breeding of the harmful microbe such as harmful bacteria, pathogenic bacteria; 5. deodorizing: the organic substance, organic sulfide, organonitrogen etc. that produce foul gas can be decomposed, greatly improve the environment in place.It is that it produces an important embodiment of multiple digestive ferment that genus bacillus can improve breeding performonce fo animals.Research shows, genus bacillus can produce multiple digestive ferment, helps animal digesting and assimilating nutritive substance.Genus bacillus has stronger proteolytic enzyme, amylase and lipase activity, also there is the enzyme of complex carbohydrates in degraded feed simultaneously, as polygalacturonase, dextranase, cellulase etc., these enzymes can destroy the cell walls of plant feed cell, the nutritive substance of cell is impelled to discharge, and the antinutritional factor can eliminated in feed, reduce the obstacle that antinutritional factor utilizes animal digestion.
Herein, the concrete subspecies of subtilis do not limit, and any one all can reach the object of the present invention.The concrete preparation method of gemma bacillus agent will be described herein-after.Aforementioned, sorbent material can be made up of organic powder of 50 ~ 70wt% and 30 ~ 50wt% inorganic powder.Herein, organic powder be selected from wheat drum, rice bran, soya bean stem meal, dregs of beans, corn cob meal, any one or two or more; Inorganic powder be selected from zeolite, diatomite, frog stone, attapulgite, light calcium carbonate, ground phosphate rock or potassium breeze any one or two or more.The bacterial classification of aforementioned genus bacillus can be made up of previously described Bacillus licheniformis, colloid bacillus cereus, subtilis and bacillus thuringiensis.First these four kinds of genus bacillus can be made respectively the microbial inoculum amount of single bacterial classification according to the method, more proportionally mix.
What deserves to be explained is, aforementioned subtilis can adopt the conventional method in this area to prepare.Such as " culture medium culturing, inoculation, fermentation " can adopt the conventional operation in this area to carry out according to actual selection.The selection being placed in substratum can adopt this area routine according to the concrete biological attribute of microorganism, and such as, substratum for genus bacillus can be peptone 10g, extractum carnis 15g, NACL15g, distilled water 1000ml, agar 18g; PH is regulated to be 7, sterilizing 20min under 0.1Mpa.
Another aspect of the invention provides the method for the microbial fertilizer preparing above-mentioned Promoting plant growth.
Prepare the method as above-mentioned microbial fertilizer, it is characterized in that, comprise the following steps:
(1) bacillus subtilis microbial agent being added to humidity is in the straw of 50 ~ 70%, and at being placed in 30 ~ 40 DEG C, aerobic fermentation 36 ~ 60h, obtains fermentable material;
(2) by same for above-mentioned fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, microbial fertilizer is obtained.
Aforementioned, term " humidity " refers to that moisture accounts for the per-cent of all-mass.In step (1), in order to build aerobic environment, in fermentation substrate, namely a cavity can be reserved in straw and grain alcohol slag.
As the order of the said raw material mixing of step of the present invention (2), do not do special restriction at this.Such as, all raw materials can be mixed in the lump, also can add a certain or certain several raw material step by step and mix.Particularly, conventional selection can be done according to the physics-chem characteristic of raw material.
In microbial fertilizer of the present invention, comprise rhamnolipid, nucleosides hydrochlorate, the two itself has somatotrophic mechanism, and compound use can play synergy further, significantly improves Plant growth-promoting effect; In addition, also can play synergy with subtilis, strengthening enzyme is lived, and improves subtilis to the degradation rate of plant straw fibers.In addition, the chitosan hydrochloride contained by microbial fertilizer of the present invention is dissolved in decomposable asymmetric choice net plant and animal residues and micro-metals after in soil, thus is converted into the nutrient substance of plant, strengthens via plant immunity, promotes the health of plant.
Embodiment
Technical scheme of the present invention is further illustrated below in conjunction with embodiment.
Embodiment 1
Following raw material is taken: 100 parts of straws, 8 parts of rhamnolipids, 4 parts of nucleosides hydrochlorates, 80 parts of chitosan hydrochlorides, 1.2 parts of bacillus subtilis microbial agents, 10 parts of lactobacillus powders, 8 parts of yeast powder, 20 parts of potassium primary phosphates, 10 parts of ferrous sulfate, 15 parts of calcium carbonate, 3 parts of magnesium chlorides, 3 parts of ammonium molybdates, 3 parts of zinc sulfate, 3 parts of manganous sulfates, 3 parts of boric acid according to mass parts.Its concrete preparation method is as follows:
Bacillus subtilis microbial agent being added to humidity is in the straw of 50%, and at being placed in 30 DEG C, aerobic fermentation 60h, obtains fermentable material.Then, by same for mentioned microorganism fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, obtain microbial fertilizer.
Embodiment 2
Following raw material is taken: 100 parts of straws, 16 parts of rhamnolipids, 10 parts of nucleosides hydrochlorates, 60 parts of chitosan hydrochlorides, 0.8 part of bacillus subtilis microbial agent, 6 parts of lactobacillus powders, 4 parts of yeast powder, 8 parts of potassium primary phosphates, 6 parts of ferrous sulfate, 10 parts of calcium carbonate, 1 part of magnesium chloride, 1 part of ammonium molybdate, 1 part of zinc sulfate, 1 part of manganous sulfate, 1 part of boric acid according to mass parts.Its concrete preparation method is as follows:
It is in the straw of 70% that bacillus subtilis microbial agent, colloid bacillus cereus microbial inoculum, bacillus amyloliquefaciens microbial inoculum are added to humidity, and at being placed in 40 DEG C, aerobic fermentation 36h, obtains fermentable material.Then, by same for mentioned microorganism fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, obtain microbial fertilizer.
Embodiment 3
Following raw material is taken: 100 parts of straws, 12 parts of rhamnolipids, 7 parts of nucleosides hydrochlorates, 80 parts of chitosan hydrochlorides, 1.2 parts of bacillus subtilis microbial agents, 10 parts of lactobacillus powders, 8 parts of yeast powder, 20 parts of potassium primary phosphates, 10 parts of ferrous sulfate, 15 parts of calcium carbonate, 3 parts of magnesium chlorides, 3 parts of ammonium molybdates, 3 parts of zinc sulfate, 3 parts of manganous sulfates, 3 parts of boric acid according to mass parts.Its concrete preparation method is as follows:
It is in the straw of 60% that bacillus subtilis microbial agent, colloid bacillus cereus microbial inoculum, bacillus amyloliquefaciens microbial inoculum are added to humidity, and at being placed in 35 DEG C, aerobic fermentation 48h, obtains fermentable material.Then, by same for mentioned microorganism fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, obtain microbial fertilizer.
Embodiment 4
Following raw material is taken: 100 parts of straws, 10 parts of rhamnolipids, 6 parts of nucleosides hydrochlorates, 70 parts of chitosan hydrochlorides, 1 part of bacillus subtilis microbial agent, 8 parts of lactobacillus powders, 6 parts of yeast powder, 12 parts of potassium primary phosphates, 8.5 parts of ferrous sulfate, 13 parts of calcium carbonate, 2 parts of magnesium chlorides, 2 parts of ammonium molybdates, 2 parts of zinc sulfate, 2 parts of manganous sulfates, 1.5 parts of boric acid according to mass parts.Its concrete preparation method is as follows:
It is in the straw of 60% that bacillus subtilis microbial agent, colloid bacillus cereus microbial inoculum, bacillus amyloliquefaciens microbial inoculum are added to humidity, and at being placed in 35 DEG C, aerobic fermentation 48h, obtains fermentable material.Then, by same for mentioned microorganism fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, obtain microbial fertilizer.
Comparative example 1
Except not containing except rhamnolipid, nucleosides hydrochlorate, other is same as embodiment 4.
By the fertilizer of embodiment 1 to 4 and comparative example in using once week about, plucking corn after corn maturation, calculating its output as following table: (represent the index of growth with per mu yield, namely per mu yield is higher, illustrate its speed grown and quality better)
The explanation of upper table, the microbial fertilizer of the embodiment of the present invention 1 to 4, does not more add the comparative example of hydroxyl Laxogenin, nucleosides hydrochlorate, can significantly improve the per mu yield of corn, its growth promotion to corn of this explanation the present invention raising speed is rhamnolipid, nucleosides hydrochlorate contributed.
Applicant states, the present invention illustrates detailed process equipment and process flow process of the present invention by above-described embodiment, but the present invention is not limited to above-mentioned detailed process equipment and process flow process, namely do not mean that the present invention must rely on above-mentioned detailed process equipment and process flow process and could implement.Person of ordinary skill in the field should understand, any improvement in the present invention, to equivalence replacement and the interpolation of ancillary component, the concrete way choice etc. of each raw material of product of the present invention, all drops within protection scope of the present invention and open scope.
Claims (6)
1. accelerate the microbial fertilizer of crop growth for one kind, it is characterized in that, its raw material comprises 100 parts of straws, 8 ~ 16 parts of rhamnolipids, 4 ~ 10 parts of nucleosides hydrochlorates, 60 ~ 80 parts of chitosan hydrochlorides, 0.8 ~ 1.2 part of bacillus subtilis microbial agent, 6 ~ 10 parts of lactobacillus powders, 4 ~ 8 parts of yeast powder, 8 ~ 20 parts of potassium primary phosphates, 6 ~ 10 parts of ferrous sulfate, 10 ~ 15 parts of calcium carbonate, 1 ~ 3 part of magnesium chloride, 1 ~ 3 part of ammonium molybdate, 1 ~ 3 part of zinc sulfate, 1 ~ 3 part of manganous sulfate, 1 ~ 3 part of boric acid according to mass parts.
2. microbial fertilizer according to claim 1, it is characterized in that, its raw material comprises 100 parts of straws, 10 parts of rhamnolipids, 6 parts of nucleosides hydrochlorates, 70 parts of chitosan hydrochlorides, 1 part of bacillus subtilis microbial agent, 8 parts of lactobacillus powders, 6 parts of yeast powder, 12 parts of potassium primary phosphates, 8.5 parts of ferrous sulfate, 13 parts of calcium carbonate, 2 parts of magnesium chlorides, 2 parts of ammonium molybdates, 2 parts of zinc sulfate, 2 parts of manganous sulfates, 1.5 parts of boric acid according to mass parts.
3. microbial fertilizer according to claim 1, it is characterized in that, if described nucleosides hydrochlorate is one in 5 '-adenylic acid (AMP) dipotassium or disodium, 5 '-bird adenylic acid (AMP) dipotassium or disodium, 5 '-cytidylic acid dipotassium or disodium, the sour dipotassium of 5 '-urine or disodium or at least two kinds.
4. microbial fertilizer according to claim 1, is characterized in that, described straw be selected from rice straw, wheat stalk, maize straw, rape stalk, cotton stalk any one or two or more.
5. microbial fertilizer according to claim 1, is characterized in that, the living bacteria count concentration of described bacillus subtilis microbial agent is 60 ~ 10,000,000,000/gram.
6. prepare a method for microbial fertilizer as claimed in claim 1, it is characterized in that, comprise the following steps:
(1) bacillus subtilis microbial agent being added to humidity is in the straw of 50 ~ 70%, and at being placed in 30 ~ 40 DEG C, aerobic fermentation 36 ~ 60h, obtains fermentable material;
(2) by same for mentioned microorganism fermentation material rhamnolipid, nucleosides hydrochlorate, chitosan hydrochloride, bacillus subtilis microbial agent, lactobacillus powder, yeast powder, potassium primary phosphate, ferrous sulfate, calcium carbonate, magnesium chloride, ammonium molybdate, zinc sulfate, manganous sulfate, boric acid mixing, microbial fertilizer is obtained.
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Cited By (10)
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| CN105993911A (en) * | 2016-05-10 | 2016-10-12 | 广西兆和种业有限公司 | Hybridization breeding method for high-yield maize |
| CN106748152A (en) * | 2016-12-23 | 2017-05-31 | 新昌县奥而特农业科技有限公司 | Composite microbiological fertilizer and preparation method thereof |
| CN106831168A (en) * | 2017-03-02 | 2017-06-13 | 山东本源生态农业科技有限公司 | A kind of biological organic fertilizer for improving crop quality and its preparation method and application |
| CN106831170A (en) * | 2017-03-03 | 2017-06-13 | 山东本源生态农业科技有限公司 | It is a kind of for turn can a fertilizer, increase conditioning agent and its preparation method and application of soil fertility |
| CN107164287A (en) * | 2017-07-12 | 2017-09-15 | 中国农业科学院农业资源与农业区划研究所 | Bacterium bacterial strain, microbial bacterial agent and its application |
| CN107211788A (en) * | 2017-05-27 | 2017-09-29 | 界首市大自然养殖专业合作社 | It is a kind of to prevent the implantation methods of lycium ruthenicum biennial bearing |
| CN107244963A (en) * | 2017-05-31 | 2017-10-13 | 新昌县南翔食品科技有限公司 | Promote fertilizer of tea growth and preparation method thereof |
| CN107759352A (en) * | 2017-11-25 | 2018-03-06 | 来安县义坤农业发展有限公司 | A kind of special fertilizer for Chinese small iris for reducing agricultural environment pollution risk |
| CN108409485A (en) * | 2018-04-03 | 2018-08-17 | 山东沃地丰生物肥料有限公司 | A kind of preparation method of composite microbe fertilizer and the composite microbe fertilizer of preparation |
| CN112830841A (en) * | 2021-01-22 | 2021-05-25 | 辽宁振兴生物科技发展有限公司 | Biological stimulator coated compound fertilizer, application and preparation method thereof |
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| CN107759352A (en) * | 2017-11-25 | 2018-03-06 | 来安县义坤农业发展有限公司 | A kind of special fertilizer for Chinese small iris for reducing agricultural environment pollution risk |
| CN108409485A (en) * | 2018-04-03 | 2018-08-17 | 山东沃地丰生物肥料有限公司 | A kind of preparation method of composite microbe fertilizer and the composite microbe fertilizer of preparation |
| CN112830841A (en) * | 2021-01-22 | 2021-05-25 | 辽宁振兴生物科技发展有限公司 | Biological stimulator coated compound fertilizer, application and preparation method thereof |
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Application publication date: 20151202 |