A kind of polynary microorganism special fertilizer preventing and treating cymbidium anthracnose and preparation method thereof
Technical field
The present invention relates to a kind of polynary microbial fertilizer, be specifically related to a kind of polynary microorganism special fertilizer preventing and treating cymbidium anthracnose and preparation method thereof, belong to technical field of fertilizers.
Background technology
Orchid is the famousr and precious seeds of flowering plants, very popular, is regarded as the foster flowers of more enervated difficulty at northern area.The requirement of current people to living environment is more and more higher, and the demand of family to orchid also gets more and more.Orchid is a kind of flowers famous with perfume (or spice), the feature that tool is noble and unsullied, elegant, famous person evaluates high to it at all times, be called and spend middle gentleman, U.S.A in ancient times often poetic prose in scholar is likened to " Lan Zhang ", being really likened to " blue friendship " friendship, and beneficial friend is likened to " blue visitor ", so orchid is famousr and precious, it is also the good merchantable brand of present.
But orchid also and difficult to support, mainly all respects such as temperature and liquid manure are difficult to control, the impact that florescence is also applied fertilizer, and easily there is anthrax when the more and temperature of watering is higher, cause very large impact to the quality of orchid, orchid is larger in the demand of florescence to fertilizer, and is afraid of dense fertilizer, if improper fertilization, plant is easily caused to burn damage to death.Conventional fertilizers for potted flowers cannot meet the various nutritive ingredients needed for orchid growth; and fertilizer efficiency is short; indoor use also can cause environmental pollution; therefore existing fertilizers for potted flowers cannot make orchid reach people's requirement; the orchid of indoor culture is not owing to changing soil for a long time; often there will be some soil mushrooms, can cause the generation of plant diseases, it is very necessary therefore to prevent and treat some common diseases.
Modern China research using microbe fertilizer has the history of nearly 50 years, also studies, applies different technology to produce polynary microbial fertilizer, namely by together polynary for multiple microbial strains in microbial fertilizer is produced.At present the research of microbial fertilizer is got more and more, and kind also gets more and more, but also do not report for the polynary microorganism special fertilizer of control cymbidium anthracnose.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of polynary microorganism special fertilizer preventing and treating cymbidium anthracnose and preparation method thereof, effectively can prevent the generation of cymbidium anthracnose, and improve its yield and quality, can also improve edaphic condition.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of polynary microorganism special fertilizer preventing and treating cymbidium anthracnose, its raw material comprises the composition of following weight ratio: bacillus megaterium 1-3 part, Streptomyces jingyangensis 3-5 part, microorganism polynary microbial inoculum 6-10 part, fertilizer 1000-1100 part, inorganic fertilizer 350-500 part, micro-fertile 30-40 part, tackiness agent 22-28 part, steady effect auxiliary agent 0.2-0.6 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described fertilizer is the climing and animal excrement of crop seedling is that 1:1-2 mixes with weight ratio, and wherein crop seedling is climing is the plant part of climbing climing crop, seedling is climing be become thoroughly decomposed cucumber, wax gourd, watermelon and summer squash seedling climing with arbitrarily than mixing; Wherein animal excrement are that pig manure, chicken manure, the cow dung become thoroughly decomposed mixes with the volume ratio of 2:3:1;
Described inorganic fertilizer is ammonium nitrate, primary ammonium phosphate, potassium sulfate mix with the weight ratio of 4-6:3-5:2-3.
Described micro-fertilizer is EDTA chelating calcium, EDTA chelated magnesium, EDTA chelated zinc, EDTA chelated iron, EDTA chelating molybdenum mix with the weight ratio of 3-5:6-8:2-4:5-7:1-3.
Described tackiness agent is a kind of or the two mixture mixed with any ratio in sodium alginate or wilkinite.
Described steady effect auxiliary agent flavonoid, tonka bean camphor, Sodium orthomolybdate, rose vitriol mix with the weight ratio of 3-4:1-3:1-2:1-2.
The preparation method of the polynary microorganism special fertilizer of above-mentioned control cymbidium anthracnose, comprises the following steps:
(1) fermentation culture of Streptomyces jingyangensis bacterial strain: first by Streptomyces jingyangensis inoculation on Streptomyces jingyangensis fermention medium, the initial pH of substratum controls at 6-8; Culture temperature controls at 27-32 DEG C; Cultivate and make bacteria suspension after 30-45 hour, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in liquid Streptomyces jingyangensis fermention medium, concussion is cultivated, and temperature controls at 27-32 DEG C, cultivates 5-7 days, final bacteria containing amount or gemma amount>=6 × 10
9individual/ml;
(2) climing for crop seedling and animal excrement are mixed with described ratio, stir, add appropriate water, adjustment water content is 55%-65%, obtains fertilizer;
(3) the polynary microbial inoculum of microorganism joins in pure water, and the weight ratio of the polynary microbial inoculum of microorganism and water is 1-2:40, after stirring, is namely obtain activation solution after placing 5-8 hour at the temperature of 20-30 DEG C in temperature;
(4) activation solution is put into the good fertilizer of pre-treatment, stir, then pile up in heaps, summer, heap highly controlled between 0.5-0.8 rice, between winter 0.8-1.2 rice, when temperature is raised to more than 50 DEG C (winter temperature is raised to 55 DEG C), start a turning, need turning be carried out later whenever piling temperature and reaching more than 65 DEG C, within 12-16 days, state of substantially becoming thoroughly decomposed can be reached;
(5) inorganic fertilizer, micro-fertilizer, tackiness agent, steady effect auxiliary agent are dissolved in water, respectively then with kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part mixing and stirring; And then the material obtained with step (4) mixes, and stirs;
(6) join in above-mentioned material by bacillus megaterium and the good Streptomyces jingyangensis of fermentation culture according to described ratio, dry through fluidized-bed, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 80 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
Described Streptomyces jingyangensis fermention medium is: glucose 1%, yeast water 2%, dipotassium hydrogen phosphate 0.02%, sodium-chlor 0.03%, and ferrous sulfate 0.004%, water supplies 100%, pH7.2-7.5.
Described bacillus megaterium originates from Anhui Hua Yang agriculture and animal husbandry Science and Technology Ltd., and Streptomyces jingyangensis bacterial strain originates from Xin Min bio tech ltd, Shanghai, and the polynary microbial inoculum of microorganism originates from Tai Yi bio tech ltd, Hebei.
The invention has the beneficial effects as follows:
(1) interpolation of bacillus megaterium can increase the content of potash fertilizer in soil, is conducive to the performance of potassium element, and make orchid branches and leaves more luxuriant, the Hua Geng great opened, more bright-coloured, beautiful, the florescence is longer.And with Streptomyces jingyangensis has certain antagonistic action to the cymbidium anthracnose in vegetative period, the generation of cymbidium anthracnose effectively can be suppressed.Streptomyces jingyangensis can also suppress the generation of other fungal disease, has the ability strengthening soil fertility, stimulate plant growth.And can strengthen plant metabolism, promote photosynthesis and strengthening blade protection film, opposing pathogenic bacteria, well developed root system time short, can also make advance flowering period 7-10 days.
(2) the polynary microbial inoculum of the microorganism used contains multiple beneficial bacterium, and after in incorporation of fertilizerin the soil, these probioticss are also bred in soil, and the mushroom in soil coexists, and reach the effect of mutual supplement with each other's advantages.And contained probiotics Fast-propagation produce macrometabolic element in substrate fermentation process, can play and prevent and treat soil-borne disease, promotes the effect of plant growth.
(3) in microbial fertilizer, add micro-fertilizer that suitable N, P, K is fertile and appropriate, effectively can solve the demand of these elements of orchid in whole vegetative period, what make that the flowers are in blossom is larger, and the florescence is longer, and the fragrance of a flower is denseer.
(4) of the present inventionly can be used for broadcasting soil treatment before rear seedling, prevent and kill off the sterilization idiocratic of farmland weed and wide spectrum.Compared with prior art, it is good, environmentally safe that distinctive formula synergy formulation of the present invention has herbicidal effect, and weeds such as not easily to develop immunity to drugs at the feature, have a extensive future.To people and animals, relatively safety is to eyes, no skin irritation in bactericidal and herbicidal agent of the present invention, and be low toxicity through day skin test checking its acute toxicity bright, easily decompose in air and soil, non-poisonous material remains; The present invention's seed dressing is little on the impact of farmland natural enemy, contributes to keeping ecotope, more friendly to environment, meets the production requirement of crop.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
embodiment 1
A kind of polynary microorganism special fertilizer preventing and treating cymbidium anthracnose, its raw material comprises the composition of following weight ratio: bacillus megaterium 1-3 part, Streptomyces jingyangensis 3-5 part, microorganism polynary microbial inoculum 6-10 part, fertilizer 1000-1100 part, inorganic fertilizer 350-500 part, micro-fertile 30-40 part, tackiness agent 22-28 part, steady effect auxiliary agent 0.2-0.6 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described fertilizer is the climing and animal excrement of crop seedling is that 1:1-2 mixes with weight ratio, and wherein crop seedling is climing is the plant part of climbing climing crop, seedling is climing be become thoroughly decomposed cucumber, wax gourd, watermelon and summer squash seedling climing with arbitrarily than mixing; Wherein animal excrement are that pig manure, chicken manure, the cow dung become thoroughly decomposed mixes with the volume ratio of 2:3:1;
Described inorganic fertilizer is ammonium nitrate, primary ammonium phosphate, potassium sulfate mix with the weight ratio of 4-6:3-5:2-3.
Described micro-fertilizer is EDTA chelating calcium, EDTA chelated magnesium, EDTA chelated zinc, EDTA chelated iron, EDTA chelating molybdenum mix with the weight ratio of 3-5:6-8:2-4:5-7:1-3.
Described tackiness agent is a kind of or the two mixture mixed with any ratio in sodium alginate or wilkinite.
Described steady effect auxiliary agent flavonoid, tonka bean camphor, Sodium orthomolybdate, rose vitriol mix with the weight ratio of 3-4:1-3:1-2:1-2.
The preparation method of the polynary microorganism special fertilizer of above-mentioned control cymbidium anthracnose, comprises the following steps:
(1) fermentation culture of Streptomyces jingyangensis bacterial strain: first by Streptomyces jingyangensis inoculation on Streptomyces jingyangensis fermention medium, the initial pH of substratum controls at 6-8; Culture temperature controls at 27-32 DEG C; Cultivate and make bacteria suspension after 30-45 hour, more in proportion 2% inoculum size by the bacterial suspension inoculation for preparing in liquid Streptomyces jingyangensis fermention medium, concussion is cultivated, and temperature controls at 27-32 DEG C, cultivates 5-7 days, final bacteria containing amount or gemma amount>=6 × 10
9individual/ml;
(2) climing for crop seedling and animal excrement are mixed with described ratio, stir, add appropriate water, adjustment water content is 55%-65%, obtains fertilizer;
(3) the polynary microbial inoculum of microorganism joins in pure water, and the weight ratio of the polynary microbial inoculum of microorganism and water is 1-2:40, after stirring, is namely obtain activation solution after placing 5-8 hour at the temperature of 20-30 DEG C in temperature;
(4) activation solution is put into the good fertilizer of pre-treatment, stir, then pile up in heaps, summer, heap highly controlled between 0.5-0.8 rice, between winter 0.8-1.2 rice, when temperature is raised to more than 50 DEG C (winter temperature is raised to 55 DEG C), start a turning, need turning be carried out later whenever piling temperature and reaching more than 65 DEG C, within 12-16 days, state of substantially becoming thoroughly decomposed can be reached;
(5) inorganic fertilizer, micro-fertilizer, tackiness agent, steady effect auxiliary agent are dissolved in water, respectively then with kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo seed coat 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part mixing and stirring; And then the material obtained with step (4) mixes, and stirs;
(6) join in above-mentioned material by bacillus megaterium and the good Streptomyces jingyangensis of fermentation culture according to described ratio, dry through fluidized-bed, temperature controls below 80 DEG C of degree, and water content control, within the scope of 10-15%, pulverizes and sieves, and fineness reaches 80 orders; Then send into roller pelletizer after crushed with travelling belt, in cylinder, uperize makes heating material to 50-60 DEG C, makes material balling-up by cylinder not stall is dynamic; Then drying roller is sent in balling-up, input hot-air seasoning, then cool, sieve, canned one-tenth finished product.
Described Streptomyces jingyangensis fermention medium is: glucose 1%, yeast water 2%, dipotassium hydrogen phosphate 0.02%, sodium-chlor 0.03%, and ferrous sulfate 0.004%, water supplies 100%, pH7.2-7.5.
Described bacillus megaterium originates from Anhui Hua Yang agriculture and animal husbandry Science and Technology Ltd., and Streptomyces jingyangensis bacterial strain originates from Xin Min bio tech ltd, Shanghai, and the polynary microbial inoculum of microorganism originates from Tai Yi bio tech ltd, Hebei.
At Beijing Chinese Academy of Sciences's Institute of Zoology, select potted plant kafir lily, potted plant butterfly orchid respectively, both arrange two process respectively, each process three repetition, each process 20 potted orchid flower, two kinds of orchid unified plantations respectively, unified management, the microbial fertilizer (contrast) that polynary microorganism fertilizer embodiment 1 obtained and Li Tu bio tech ltd, Hebei produce be applied to respectively kafir lily, butterfly orchid, embodiment is identical with the fertilizer amount of contrast.Respectively probiotics content and contrast in the size anthrax sickness rate of its flowering duration, flower, soil are compared, obtain its comparing result.
The comparing result of potted plant kafir lily is concrete as table 1:
Microbial fertilizer prepared by table 1 the present invention and contrast fertilizer compare (increment %)
|
Flowering duration (%) |
The size (%) of flower |
Anthrax reduction (%) |
Soil probiotics increment (%) |
Embodiment 1 |
22 |
13 |
70 |
36 |
The comparing result of potted plant butterfly orchid is in table 2:
Microbial fertilizer prepared by table 2 the present invention and contrast fertilizer compare (increment %)
|
Flowering duration (%) |
The size (%) of flower |
Anthrax reduction (%) |
Soil probiotics increment (%) |
Embodiment 1 |
30 |
16 |
66 |
32 |
Learn through comparing, the special polynary microorganism fertilizer of orchid prepared by the present invention, greatly can reduce the generation of cymbidium anthracnose, and the probiotics quantity in soil can be made to increase, it is larger to make that the flowering duration of orchid is longer, flower amount is more, the flowers are in blossom, and visual inspection, flower is more bright-coloured.
Seedling rate of the present invention on average reaches 83%, and contrast treatment reaches conspicuous level, is 77.6% to the inhibiting rate of common disease.
Of the present invention through field comparison test, all obtain positive effect, main manifestations is as follows: containing abundant organic and humic acid, can improve Soil structure, vegetable soil, promote that soil is built up fertility.With other fertilizer ratio such as nutrient such as grade, crop yield 22%.Formulate and coordinate farm crop and absorb nutrition, stimulate and regulation and control plant growth, reduce or reduce crop pest, affecting crop quality.Or fertilizer amount need not be reduced, be beneficial to protection of the environment.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.