A kind of special-purpose fertilizer of polynary microorganism of preventing and treating cymbidium anthracnose and preparation method thereof
Technical field
The present invention relates to a kind of polynary microbial fertilizer, be specifically related to a kind of special-purpose fertilizer of polynary microorganism of preventing and treating cymbidium anthracnose and preparation method thereof, belong to technical field of fertilizers.
Background technology
Orchid is the famousr and precious seeds of flowering plants, and is very popular, is regarded as the flowers that more enervated difficulty is supported at northern area.People are more and more higher to the requirement of living environment at present, and family is also more and more to the demand of orchid.Orchid is a kind of flowers that are celebrated with perfume (or spice), the characteristics that tool is noble and unsullied, elegant, the famous person estimates high to it at all times, be called and spend middle gentleman, often U.S. of poetic prose analogy is " Lan Zhang " among the scholar in ancient times, the true analogy of friendship is " the blue friendship ", beneficial friend's analogy is " blue visitor ", so orchid is famousr and precious, it also is the good merchantable brand of present.
Yet orchid also and difficult supporting, mainly be that all respects such as temperature and liquid manure are difficult to control, the influence that florescence is also applied fertilizer, and the more and temperature of watering higher situation under anthrax easily takes place, quality to orchid causes very big influence, and orchid is bigger to the demand of fertilizer at the florescence, and is afraid of dense fertilizer, if improper fertilization causes plant to burn damage to death easily.Conventional fertilizers for potted flowers can't satisfy the required various nutritive ingredients of orchid growth; and fertilizer efficiency is short; indoor use also can cause environmental pollution; therefore existing fertilizers for potted flowers can't make orchid reach people's requirement; the orchid of indoor culture is not owing to change soil for a long time; therefore some soil mushrooms appear in regular meeting, can cause the generation of plant diseases, and it is very necessary to prevent and treat some common diseases.
The existing nearly 50 years history of modern China's research using microbe fertilizer has also been studied, has been used different technology and produced polynary microbial fertilizer in microbial fertilizer production, just that a plurality of microbial strainss are together polynary.Research to microbial fertilizer at present gets more and more, and kind is also more and more, but does not also have report at the special-purpose fertilizer of the polynary microorganism of control cymbidium anthracnose.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of special-purpose fertilizer of polynary microorganism of preventing and treating cymbidium anthracnose and preparation method thereof, can effectively prevent the generation of cymbidium anthracnose, and improve its yield and quality, can also improve edaphic condition.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of special-purpose fertilizer of polynary microorganism of preventing and treating cymbidium anthracnose, its raw material comprises the composition of following weight ratio: bacillus megaterium 1-3 part, Jingyang streptomycete 3-5 part, the polynary microbial inoculum 6-10 of microorganism part, fertilizer 1000-1100 part, inorganic fertilizer 350-500 part, little fertile 30-40 part, tackiness agent 22-28 part, steady effect auxiliary agent 0.2-0.6 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described fertilizer is that the climing and animal excrement of crop seedling are that 1:1-2 mixes with the weight ratio, and wherein the crop seedling is climing is the plant part of climbing climing crop, seedling is climing be the cucumber, wax gourd, watermelon and the summer squash seedling that become thoroughly decomposed climing with any than mixing; Wherein animal excrement are that pig manure, chicken manure, the cow dung of becoming thoroughly decomposed mixes with the volume ratio of 2:3:1;
Described inorganic fertilizer is that ammonium nitrate, primary ammonium phosphate, vitriolate of tartar mix with the weight ratio of 4-6:3-5:2-3.
Described little fertilizer is that EDTA chelating calcium, EDTA chelated magnesium, EDTA chelating zinc, EDTA chelated iron, EDTA chelating molybdenum mix with the weight ratio of 3-5:6-8:2-4:5-7:1-3.
Described tackiness agent is a kind of or the two mixture with any mixed in sodium alginate or the wilkinite.
Described steady effect auxiliary agent flavonoid, tonka bean camphor, Sodium orthomolybdate, rose vitriol mix with the weight ratio of 3-4:1-3:1-2:1-2.
The special-purpose fertile preparation method of the polynary microorganism of above-mentioned control cymbidium anthracnose may further comprise the steps:
(1) fermentation culture of Jingyang streptomycete bacterial strain: at first the Jingyang streptomycete bacterial strain is inoculated on the streptomycete fermentation substratum of Jingyang, the initial pH control of substratum is at 6-8; Culture temperature control is at 27-32 ℃; Cultivate and make bacteria suspension after 30-45 hour, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the streptomycete fermentation substratum of liquid Jingyang, concussion is cultivated, temperature control at 27-32 ℃, cultivates 5-7 days, finally bacteria containing amount or gemma amount 〉=6 * 10
9Individual/ml;
(2) with the climing and animal excrement of crop seedling with described mixed, stir, add an amount of water, the adjustment water content is 55%-65%, obtains fertilizer;
(3) the polynary microbial inoculum of microorganism joins in the pure water, and the weight ratio of the polynary microbial inoculum of microorganism and water is 1-2:40, after stirring, is to place after 5-8 hour under 20-30 ℃ the temperature namely to get activation solution in temperature;
(4) activation solution is put into the good fertilizer of pre-treatment, stir, pile up in heaps then, the high control of heap in summer is between 0.5-0.8 rice, between the winter 0.8-1.2 rice, treat that temperature is raised to 50 ℃ (winter temperature is raised to 55 ℃) and begins a turning when above, when the heap temperature reaches more than 65 ℃, need carry out turning later on, can reach the state that becomes thoroughly decomposed substantially in 12-16 days;
(5) with inorganic fertilizer, little fertilizer, tackiness agent, surely imitate auxiliary agent and be dissolved in water respectively, then with kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part mixing and stirring; And then mix with material that step (4) makes, stir;
(6) bacillus megaterium and the good Jingyang streptomycete of fermentation culture are joined in the above-mentioned material according to described ratio, through fluidized-bed oven dry, temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 80 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
Described Jingyang streptomycete fermentation substratum is: glucose 1%, and yeast water 2%, dipotassium hydrogen phosphate 0.02%, sodium-chlor 0.03%, ferrous sulfate 0.004%, water is supplied 100%, pH7.2-7.5.
Described bacillus megaterium originates from Anhui China raises agriculture and animal husbandry Science and Technology Ltd., and the Jingyang streptomycete bacterial strain originates from prosperous Min, Shanghai bio tech ltd, and the polynary microbial inoculum of microorganism originates from too easily bio tech ltd of Hebei.
The invention has the beneficial effects as follows:
(1) interpolation of bacillus megaterium can increase the content of potash fertilizer in the soil, is conducive to the performance of potassium element, makes the orchid branches and leaves more luxuriant, the Hua Gengda that opens, and more bright-coloured, beautiful, the florescence is longer.And with the Jingyang streptomycete cymbidium anthracnose in vegetative period is had certain antagonistic action, can effectively suppress the generation of cymbidium anthracnose.The Jingyang streptomycete can also suppress the generation of other fungal disease, has the ability that strengthens soil fertility, stimulates plant growth.And can strengthen plant metabolism, promote photosynthesis and strengthen the blade protection film, the opposing pathogenic bacteria, well developed root system when short can also make advance flowering period 7-10 days.
(2) the polynary microbial inoculum of employed microorganism contains the multiple beneficial bacterium, in incorporation of fertilizerin the soil after, these probioticss also in soil, breed and soil in mushroom coexistence, reach the effect of mutual supplement with each other's advantages.And contained probiotics is bred fast in the substrate fermentation process and is produced macrometabolic element, can play and prevent and treat soil-borne disease, promotes the effect of plant growth.
(3) added suitable N, P, K fertilizer and an amount of little fertilizer in microbial fertilizer, can effectively solve the demand of whole growth phase these elements of orchid, made bigger that the flowers are in blossom, the florescence is longer, and the fragrance of a flower is denseer.
(4) of the present inventionly can be used for broadcasting soil treatment before the seedling of back, prevent and kill off the sterilization idiocratic of farmland weed and wide spectrum.Compared with prior art, it is good, environmentally safe that distinctive prescription synergy formulation of the present invention has a herbicidal effect, and weeds characteristics such as be difficult for developing immunity to drugs have a extensive future.Bactericidal and herbicidal agent of the present invention relatively safely to eyes, skin nonirritant, is low toxicity through bright its acute toxicity of day skin test checking to people and animals, easily decomposes in atmosphere and soil, and non-poisonous material is residual; The present invention's seed dressing is little to the influence of farmland natural enemy, helps to keep ecotope, and is more friendly to environment, satisfies the production requirement of crop.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
A kind of special-purpose fertilizer of polynary microorganism of preventing and treating cymbidium anthracnose, its raw material comprises the composition of following weight ratio: bacillus megaterium 1-3 part, Jingyang streptomycete 3-5 part, the polynary microbial inoculum 6-10 of microorganism part, fertilizer 1000-1100 part, inorganic fertilizer 350-500 part, little fertile 30-40 part, tackiness agent 22-28 part, steady effect auxiliary agent 0.2-0.6 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described fertilizer is that the climing and animal excrement of crop seedling are that 1:1-2 mixes with the weight ratio, and wherein the crop seedling is climing is the plant part of climbing climing crop, seedling is climing be the cucumber, wax gourd, watermelon and the summer squash seedling that become thoroughly decomposed climing with any than mixing; Wherein animal excrement are that pig manure, chicken manure, the cow dung of becoming thoroughly decomposed mixes with the volume ratio of 2:3:1;
Described inorganic fertilizer is that ammonium nitrate, primary ammonium phosphate, vitriolate of tartar mix with the weight ratio of 4-6:3-5:2-3.
Described little fertilizer is that EDTA chelating calcium, EDTA chelated magnesium, EDTA chelating zinc, EDTA chelated iron, EDTA chelating molybdenum mix with the weight ratio of 3-5:6-8:2-4:5-7:1-3.
Described tackiness agent is a kind of or the two mixture with any mixed in sodium alginate or the wilkinite.
Described steady effect auxiliary agent flavonoid, tonka bean camphor, Sodium orthomolybdate, rose vitriol mix with the weight ratio of 3-4:1-3:1-2:1-2.
The special-purpose fertile preparation method of the polynary microorganism of above-mentioned control cymbidium anthracnose may further comprise the steps:
(1) fermentation culture of Jingyang streptomycete bacterial strain: at first the Jingyang streptomycete bacterial strain is inoculated on the streptomycete fermentation substratum of Jingyang, the initial pH control of substratum is at 6-8; Culture temperature control is at 27-32 ℃; Cultivate and make bacteria suspension after 30-45 hour, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the streptomycete fermentation substratum of liquid Jingyang, concussion is cultivated, temperature control at 27-32 ℃, cultivates 5-7 days, finally bacteria containing amount or gemma amount 〉=6 * 10
9Individual/ml;
(2) with the climing and animal excrement of crop seedling with described mixed, stir, add an amount of water, the adjustment water content is 55%-65%, obtains fertilizer;
(3) the polynary microbial inoculum of microorganism joins in the pure water, and the weight ratio of the polynary microbial inoculum of microorganism and water is 1-2:40, after stirring, is to place after 5-8 hour under 20-30 ℃ the temperature namely to get activation solution in temperature;
(4) activation solution is put into the good fertilizer of pre-treatment, stir, pile up in heaps then, the high control of heap in summer is between 0.5-0.8 rice, between the winter 0.8-1.2 rice, treat that temperature is raised to 50 ℃ (winter temperature is raised to 55 ℃) and begins a turning when above, when the heap temperature reaches more than 65 ℃, need carry out turning later on, can reach the state that becomes thoroughly decomposed substantially in 12-16 days;
(5) with inorganic fertilizer, little fertilizer, tackiness agent, surely imitate auxiliary agent and be dissolved in water respectively, then with kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part mixing and stirring; And then mix with material that step (4) makes, stir;
(6) bacillus megaterium and the good Jingyang streptomycete of fermentation culture are joined in the above-mentioned material according to described ratio, through fluidized-bed oven dry, temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 80 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
Described Jingyang streptomycete fermentation substratum is: glucose 1%, and yeast water 2%, dipotassium hydrogen phosphate 0.02%, sodium-chlor 0.03%, ferrous sulfate 0.004%, water is supplied 100%, pH7.2-7.5.
Described bacillus megaterium originates from Anhui China raises agriculture and animal husbandry Science and Technology Ltd., and the Jingyang streptomycete bacterial strain originates from prosperous Min, Shanghai bio tech ltd, and the polynary microbial inoculum of microorganism originates from too easily bio tech ltd of Hebei.
In plant research institute of BeiJing ZhongKe institute, select potted plant kafir lily, potted plant butterfly orchid respectively, both arrange two processing respectively, each handles three repetitions, each handles 20 potted orchids flower, two kinds of orchid unified plantations respectively, unified management, the microbial fertilizer (contrast) that the polynary microorganism fertilizer that embodiment 1 is obtained and Hebei sharp autochthonal thing Science and Technology Ltd. produces be applied to respectively kafir lily,, butterfly orchid, the fertilizer amount of embodiment and contrast is identical.Respectively it being bloomed, probiotics content and contrast compare in the big or small anthrax sickness rate, soil of time length, flower, obtain its comparing result.
The comparing result of potted plant kafir lily is specifically as table 1:
The microbial fertilizer of table 1 the present invention preparation and contrast fertilizer is (increment %) relatively
|
Bloom the time length (%) |
The size (%) of flower |
Anthrax reduction (%) |
Soil probiotics increment (%) |
Embodiment 1 |
22 |
13 |
70 |
36 |
The comparing result of potted plant butterfly orchid sees Table 2:
The microbial fertilizer of table 2 the present invention preparation and contrast fertilizer is (increment %) relatively
|
Bloom the time length (%) |
The size (%) of flower |
Anthrax reduction (%) |
Soil probiotics increment (%) |
Embodiment 1 |
30 |
16 |
66 |
32 |
Through relatively learning, the special-purpose polynary microorganism fertilizer of the orchid of the present invention's preparation, can reduce the generation of cymbidium anthracnose greatly, and the probiotics quantity in the soil is increased, can also make the time length of blooming of orchid longer, spend bigger that amount is more, the flowers are in blossom, and visual inspection, flower is more bright-coloured.
Seedling rate of the present invention on average reaches 83%, and contrast treatment reaches conspicuous level, is 77.6% to the inhibiting rate of common disease.
Of the present invention through the field simultaneous test, all obtain positive effect, mainly show as follows: contain and enrich organic matter and humic acid, can improve Soil structure, vegetable soil promotes soil to build up fertility.With other fertilizer ratio such as nutrient such as grade, crop yield 22%.Formulation and the nutrition of coordination crop absorption stimulate and the regulation and control plant growth, reduce or the reduction crop pest, influence crop quality.Need not or reduce fertilizer amount, be beneficial to the protection environment.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.