CN103224422A - Compatibility of hericium erinaceus culture material and manufacturing method of culture material - Google Patents

Compatibility of hericium erinaceus culture material and manufacturing method of culture material Download PDF

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CN103224422A
CN103224422A CN2013101751889A CN201310175188A CN103224422A CN 103224422 A CN103224422 A CN 103224422A CN 2013101751889 A CN2013101751889 A CN 2013101751889A CN 201310175188 A CN201310175188 A CN 201310175188A CN 103224422 A CN103224422 A CN 103224422A
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hericium erinaceus
pers
bull
vinegar
culture material
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CN103224422B (en
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邬金飞
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Abstract

The invention relates to a hericium erinaceus culture material which is characterized in that leftovers in the brewing industry, namely vinegar residue and broad bean hull are taken as main raw materials, and the water content in the culture material is 63%-65%. The invention also relates to a manufacturing method of the culture material. Compared with the existing culture material, the hericium erinaceus culture material has the following advantages: 1) the materials are taken locally, so that the production cost is reduced; 2) the acidity of the vinegar residue raw material is appropriate, the nutrition is rich, and after the vinegar residue is matched with the broad bean hull, the culture material has the advantages of good air permeability, good water retention property, luxuriant growth of mycelia, tidy growth of fruiting bodies, high yield and good quality; and 3) by utilizing the vinegar residue to culture hericium erinaceus, the channels of the culture raw materials are expanded, the utilization rate of resources is improved, and the economic income of a brewing enterprise is simultaneously increased.

Description

The making method of a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material compatibility and this cultivation material
Technical field
Content of the present invention comprises Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material compatibility, and the making method of this cultivation material, belongs to the fungus growing technique field.
Background technology
Have the hedgehog hydnum of the title of " mountain delicacy ", nutritious, be famous nourishing body-strengening food, also have higher pharmaceutical use simultaneously, the tumour and the inflammation of some Digestive tract there is very high curative ratio.
Hericium erinaceus (Bull. Ex Fr.) Pers. artificial culture materials mostly are cotton seed hulls greatly at present, hardwood sawdust etc., fast development along with mushroom industry, production-scale expansion, Hericium erinaceus (Bull. Ex Fr.) Pers. produces the problem that also faces shortage of raw materials and production cost rising, therefore each production unit is all attached great importance to the looking for of cheap and good-quality alternative materials, and screening is suitable for the cultivation major ingredient that Hericium erinaceus (Bull. Ex Fr.) Pers. grows with suiting measures to local conditions.
In recent years, because human consumer's standard of living improves constantly, the vinegar of solid state fermentation production is well received by consumers, for meeting the need of market, its output constantly increases, and also rolls up but the discarded vinegar that the consequent is in the production process to be produced is poor, and is in need of immediate treatment.
Vinegar is poor to be residue after raw materials such as corn, jowar, wheat, broad bean are made vinegar, be the main tankage of making vinegar, it contains crude protein 6%-10%, crude fat 2%-5%, nitrogen-free extract 20%-30%, coarse ash 13%-17%, calcium 0.25%-0.45%, phosphorus 0.16%-0.37%, acid higher (pH is about 4), nutritious; The main component of tankage broad bean skin is Mierocrystalline cellulose, hemicellulose, xylogen, still contains certain mineral substance, protein, VITAMIN etc. in addition, and wherein Mierocrystalline cellulose 56.8%, hemicellulose 11.8%, xylogen 8.7%, crude protein 4.64%, structural protein 4.12%.
Hericium erinaceus (Bull. Ex Fr.) Pers. belongs to the acid mushroom of happiness, and vegetative stage is with the pH4 optimum, and the sporophore growth stage is with the pH4-5 optimum.The required nutrient of the poor nutritive ingredient of broad bean skin, vinegar and Hericium erinaceus (Bull. Ex Fr.) Pers. is very identical, so is that Hericium erinaceus (Bull. Ex Fr.) Pers. is cultivated comparatively ideal raw material.
Utilize these tankage to be not only this behave of falling of an Edible Fungi, but also be a circular agriculture and environmental protection cause behave.
Summary of the invention
The present invention has following two aspect contents:
1, a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material compatibility
2, the making method of cultivation material
The technical solution adopted in the present invention is:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material compatibility has following component and quality proportioning: the vinegar 40%-50% that is pickled with grains or in wine, and broad bean skin 25%-35%, wheat bran 10%-20%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2% all is the quality of dry-matter, the proportioning sum is 100%.
Vinegar is poor: fresh vinegar is poor, and is acid higher, be spread out on the concrete floor or on the plastics film to ted, and tart flavour is vapored away as far as possible, and moisture content is dropped to below the 12%-15%, dries standby.
The broad bean skin through drying, is ground into 5mm following (particle diameter), and is standby.
Wheat bran: remaining wheat skin during for wheat flour milling.
Semen Maydis powder, calcium superphosphate, gypsum are common commercially available product.
The making method of cultivation material comprises the steps:
1. prewet: vinegar is pickled with grains or in wine mixes, prewetted 2 hours, make the water content of mixture remain on 50%-55% with broad bean skin siccative;
2. spice: with wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, join in the mixture of having prewetted, stir, then slowly add water, the limit edged is mixed, and fully stirs evenly, and makes the water content of cultivation material remain on 63%-65%;
3. bottle, beat and plant the bacterium hole: will cultivate and expect to pack into 1100ml * 78mmPP Plastic Bottle, and stamp and plant the bacterium hole, planting aperture, bacterium hole is 16mm, covers the bottle cap of the ventilative sponge of band then;
4. sterilize, cool off: will cultivate material and carry out autoclaving, and at 100 ℃ of 1h that sterilize down, rise to 121 ℃ of sterilization 1.5h then earlier, and naturally cool to after the sterilization below 25 ℃;
The cultivation material is after inserting bacterial classification, and culture temperature is controlled at 20 ℃~22 ℃, and humidity is controlled at 60%~75%, and through the cultivation of 20d~23d, mycelia is covered with bottle, can enter management of producing mushroom.
The present invention is poor with Brewing industry tankage vinegar, the broad bean skin is a main raw material, makes Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material.Its advantage is: the one, gather materials on the spot, and reduced production cost; The 2nd, the poor feedstock acidity of vinegar is suitable, comprehensive nutrition, with the collocation of broad bean skin after, cultivation material ventilation property, retentiveness are good, the mycelia growing way is vigorous, sporophore growth is neat, output height, quality better; The 3rd, utilize poor slag for cultivating Hericium erinaceus (Bull. Ex Fr.) Pers., widened the culturing raw material channel, improved resource utilization, increased the income of brewageing enterprise simultaneously.
Embodiment
Mode 1: Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material package is drawn together following component and quality proportioning in the present embodiment: vinegar is pickled with grains or in wine 40%, broad bean skin 30%, and wheat bran 20%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2% all is the quality of dry-matter, the proportioning sum is 100%.
Making method:
1. prewet: vinegar is pickled with grains or in wine mixes, prewetted 2 hours, make the water content of mixture remain on 50%-55% with broad bean skin siccative;
2. spice: with wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, join in the mixture of having prewetted, stir, then slowly add water, the limit edged is mixed, and fully stirs evenly, and makes the water content of cultivation material remain on 63%-65%;
3. bottle, beat and plant the bacterium hole: will cultivate and expect to pack into 1100ml * 78mmPP Plastic Bottle, and stamp and plant the bacterium hole, planting aperture, bacterium hole is 16mm, covers the bottle cap of the ventilative sponge of band then;
4. sterilize, cool off: will cultivate material and carry out autoclaving, and at 100 ℃ of 1h that sterilize down, rise to 121 ℃ of sterilization 1.5h then earlier, and naturally cool to after the sterilization below 25 ℃;
The cultivation material is after inserting bacterial classification, and culture temperature is controlled at 20 ℃~22 ℃, and humidity is controlled at 60%~75%, and through the cultivation of 20d~23d, mycelia is covered with bottle, can enter management of producing mushroom.
Mode 2: Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material package is drawn together following component and quality proportioning in the present embodiment: vinegar is pickled with grains or in wine 46%, broad bean skin 28%, and wheat bran 16%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2% all is the quality of dry-matter, the proportioning sum is 100%.Making method reference example 1.
Mode 3: Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material package is drawn together following component and quality proportioning in the present embodiment: vinegar is pickled with grains or in wine 48%, broad bean skin 32%, and wheat bran 10%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2% all is the quality of dry-matter, the proportioning sum is 100%.Making method reference example 1.
Mode 4 (control group 1): Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material package is drawn together following component and quality proportioning: cotton seed hulls 78%, and wheat bran 20%, calcium superphosphate 1%, terra alba 1% all is the quality of dry-matter, the proportioning sum is 100%.
Mode 5 (control group 2): Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material package is drawn together following component and quality proportioning: wood chip 78%, and wheat bran 20%, calcium superphosphate 1%, terra alba 1% all is the quality of dry-matter, the proportioning sum is 100%.
From table as can be known: the poor with vinegar of all modes is the prescription group of major ingredient, mycelial growth rate obviously is better than control group, full bottle fate generally shortens, and this may be the utilization that the poor nutrition that contains of vinegar is more conducive to Hericium erinaceus (Bull. Ex Fr.) Pers., and also there is the chemical ingredients that stimulates hericium mycelium to grow in possibility vinegar in being pickled with grains or in wine; All modes with vinegar poor be the prescription group biology efficient of major ingredient be significantly higher than with the wood chip be major ingredient the prescription group, be close with the prescription group that with the cotton seed hulls is major ingredient.
The cultivation material of table different ingredients is to the influence of Hericium erinaceus (Bull. Ex Fr.) Pers. output
Figure BSA00000893730700031

Claims (5)

1. Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material is characterized in that containing following component and quality proportioning: the vinegar 40%-50% that is pickled with grains or in wine, broad bean skin 25%-35%, wheat bran 10%-20%, Semen Maydis powder 6%, calcium superphosphate 2%, gypsum 2% all is the quality of dry-matter, and the proportioning sum is 100%.
2. Hericium erinaceus (Bull. Ex Fr.) Pers. according to claim 1 cultivation material is characterized in that described vinegar is poor and is the waste after brewery's wine vinegar.
3. Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material according to claim 1 is characterized in that described broad bean skin through drying, and is ground into 5mm following (particle diameter).
4. the making method of the cultivation of the Hericium erinaceus (Bull. Ex Fr.) Pers. described in the claim 1~3 material is characterized in that:
1. prewet: vinegar is pickled with grains or in wine mixes, prewetted 2 hours, make the water content of mixture remain on 50%-55% with broad bean skin siccative;
2. spice: with wheat bran, Semen Maydis powder, calcium superphosphate, gypsum, join in the mixture of having prewetted, stir, then slowly add water, the limit edged is mixed, and fully stirs evenly, and makes the water content of cultivation material remain on 63%-65%;
3. bottle, beat and plant the bacterium hole: will cultivate and expect to pack into 1100ml * 78mmPP Plastic Bottle, and stamp and plant the bacterium hole, planting aperture, bacterium hole is 16mm, covers the bottle cap of the ventilative sponge of band then;
4. sterilize, cool off: will cultivate material and carry out autoclaving, and at 100 ℃ of 1h that sterilize down, rise to 121 ℃ of sterilization 1.5h then earlier, and naturally cool to after the sterilization below 25 ℃.
5. with the Hericium erinaceus (Bull. Ex Fr.) Pers. cultivation material described in the claim 1-4, after it is characterized in that inserting cultivar, culture temperature is controlled at 20 ℃~22 ℃, and humidity is controlled at 60%~75%, and through the cultivation of 20d~23d, mycelia is covered with bottle, can enter management of producing mushroom.
CN201310175188.9A 2013-04-28 2013-04-28 Compatibility of hericium erinaceus culture material and manufacturing method of culture material Expired - Fee Related CN103224422B (en)

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Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103408368A (en) * 2013-08-03 2013-11-27 邬金飞 Compatibility and manufacturing method of Hericium erinaceus cultivation material
CN104086259A (en) * 2014-06-27 2014-10-08 方小超 Organic fertilizer material for cultivating hericium erinaceus
CN104193465A (en) * 2014-07-24 2014-12-10 安徽天都灵芝制品公司 Hericium erinaceus culture medium using broad-leaved tree sawdust as raw material and preparation method thereof
CN104303819A (en) * 2014-09-03 2015-01-28 四川大学 Method for cultivating hericium erinaceus with fresh vinegar residues
CN104557175A (en) * 2015-01-28 2015-04-29 邬金飞 Production method of hericium erinaceus cultivation material
CN104609987A (en) * 2015-02-24 2015-05-13 邬方成 Method for manufacturing agrocybe cylindracea cultivation material
CN104725114A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104725120A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104725112A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104725113A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104725121A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104725122A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104744155A (en) * 2015-03-06 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104744157A (en) * 2015-03-07 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104744156A (en) * 2015-03-07 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104756757A (en) * 2015-03-09 2015-07-08 邬方成 Method for planting hericium erinaceus
CN104945055A (en) * 2015-06-19 2015-09-30 桂林健成生物科技开发有限公司 Application of seed coats/embryonic bodies and rhizomes obtained after harvest of sprout seedling vegetable to cultivation of hericium erinaceus
CN104956917A (en) * 2015-06-29 2015-10-07 吴华球 Culture method for hericium erinaceus
CN107721615A (en) * 2017-11-15 2018-02-23 陈荣立 Pleurotus cornucopiae cultivation matrix and preparation method thereof

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Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103408368A (en) * 2013-08-03 2013-11-27 邬金飞 Compatibility and manufacturing method of Hericium erinaceus cultivation material
CN103408368B (en) * 2013-08-03 2014-12-24 邬金飞 Compatibility and manufacturing method of Hericium erinaceus cultivation material
CN104086259A (en) * 2014-06-27 2014-10-08 方小超 Organic fertilizer material for cultivating hericium erinaceus
CN104193465A (en) * 2014-07-24 2014-12-10 安徽天都灵芝制品公司 Hericium erinaceus culture medium using broad-leaved tree sawdust as raw material and preparation method thereof
CN104303819A (en) * 2014-09-03 2015-01-28 四川大学 Method for cultivating hericium erinaceus with fresh vinegar residues
CN104303819B (en) * 2014-09-03 2016-05-04 四川大学 A kind of method of utilizing fresh vinegar grain cultivation Hericium erinaceus
CN104557175A (en) * 2015-01-28 2015-04-29 邬金飞 Production method of hericium erinaceus cultivation material
CN104725113A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104609987A (en) * 2015-02-24 2015-05-13 邬方成 Method for manufacturing agrocybe cylindracea cultivation material
CN104725112A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104725114A (en) * 2015-02-24 2015-06-24 邬方成 Preparation method of agrocybe aegirit cultivation material
CN104725121A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104725122A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104725120A (en) * 2015-03-02 2015-06-24 邬方成 Preparation method of hericium erinaceus cultivation material
CN104744155A (en) * 2015-03-06 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104744157A (en) * 2015-03-07 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104744156A (en) * 2015-03-07 2015-07-01 邬方成 Method for manufacturing agrocybe cylindracea compost
CN104756757A (en) * 2015-03-09 2015-07-08 邬方成 Method for planting hericium erinaceus
CN104945055A (en) * 2015-06-19 2015-09-30 桂林健成生物科技开发有限公司 Application of seed coats/embryonic bodies and rhizomes obtained after harvest of sprout seedling vegetable to cultivation of hericium erinaceus
CN104956917A (en) * 2015-06-29 2015-10-07 吴华球 Culture method for hericium erinaceus
CN107721615A (en) * 2017-11-15 2018-02-23 陈荣立 Pleurotus cornucopiae cultivation matrix and preparation method thereof

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