CN104725121A - Preparation method of hericium erinaceus cultivation material - Google Patents

Abstract

The invention provides a preparation method of a hericium erinaceus cultivation material, which is technology for applying lentinus edodes stalk feet to hericium erinaceus cultivation. Compared with the prior art, the preparation method has the advantages that I a lentinus edodes stalk foot soaking material is a pure natural nutrient solution and contains a wide variety of bioactive substances, so that beneficial microbe reproduction is promoted, microbial communities in the material are improved and catalytic decomposition of the cultivation material is accelerated by blending the lentinus edodes stalk foot soaking material with the cultivation raw materials to carry out piling fermentation; II the fermented cultivation material contains high-activity beneficial bacteria and active metabolites thereof, has a promotion effect on growth of hericium erinaceus hyphae and has strong hypha growth vigour and foreign bacterium resistance; III the resources, such as manpower and coal, are saved and the environmental pollution is reduced, so that the preparation method accords with the low-carbon environmental protection concept.

Description

A kind of making method of Hericium erinaceus culture material

Technical field

The making method of a kind of Hericium erinaceus culture material of the present invention, is the technology be applied to by mushroom tang in Hericium erinaceus culture, belongs to fungus growing technique field.

Background technology

Have the hedgehog hydnum of the title of " mountain delicacy ", nutritious, be famous nourishing body-strengening food, also there is higher pharmaceutical use simultaneously, have very high curative ratio to the tumour of some Digestive tract and inflammation.

The traditional cultivating method of Hericium erinaceus (Bull. Ex Fr.) Pers. is grog cultivation, and planting material carries out inoculation culture after high pressure or normal-pressure sterilization, and now in material, the macromolecule organic such as Mierocrystalline cellulose has been degraded, miscellaneous bacteria in material is also killed, therefore, when planting material sends out bacterium, mycelial growth is fast, and living contaminants is few.But along with the significantly rise of the price of labour power and fuel price, grog cultivation cost increases severely, and high-temperature sterilization also causes the loss of planting material nutritive substance, causes biological transformation ratio to reduce simultaneously.

Mushroom is famous and precious edible one of the medicinal fungus of holding concurrently in the world, and current China mushroom production accounts for 70% of world's mushroom ultimate production, has the equal mushroom culture in the province of more than 70% in China.Because champignon stems stiffness of foot in children, content of cellulose are higher, palatability is poor, and therefore, mushroom is being eaten raw or in the course of processing, the tang of mushroom can remove as tankage by the producer, causes the mushroom tang accounting for mushroom weight about 30% to become waste.

Summary of the invention

For the deficiencies in the prior art, the present invention's mushroom tang soaks expects to mix and stir to carry out banking up with planting material to ferment, and do not add sterilant, sterilant during windrow, the planting material after fermentation directly packs inoculation, bacterium bag living contaminants is few, and the Hericium erinaceus (Bull. Ex Fr.) Pers. quality of producing is good, output is high.

The technical solution adopted in the present invention is:

A making method for Hericium erinaceus culture material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

(1) prepare burden: bagasse 46-52%, wood chip 28-32%, soya-bean cake 14-18%, mushroom tang 3-4%, potassium primary phosphate 0.1%, calcium superphosphate 0.4%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described bagasse powder is broken into the particle of particle diameter 3mm-5mm; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in bagasse, wood chip, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keep 24h, then turning is carried out, and turning every day later once, during first time turning, add gypsum, during second time turning, add calcium superphosphate, potassium primary phosphate, turning 3-4 time altogether, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, and bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 24-26d, mycelia covers with pocket, enters management of producing mushroom routinely.

Mushroom tang is applied to the method for Hericium erinaceus culture by the present invention, and apart from outside explanation, other operation steps is all undertaken by this area routine techniques.

The present invention's advantage is compared with prior art:

1. mushroom tang leach liquor is full-natural nutritive liquid, containing glucide, amino acid, peptide class, nucleic acid and trace element, bio-hormone also containing abundant species, also the biologically active substance do not leached in a large number is had in champignon stems slag, soak with mushroom tang and expect to mix and stir to carry out banking up with culturing raw material to ferment, promote beneficial microorganism breeding, improve the microorganism species in material, accelerate the catalytic decomposition of planting material;

2. the planting material after fermentation has high reactivity probiotics and active metabolite thereof, has accelerating effect, show as prosperous, the anti-miscellaneous bacteria ability of mycelium growth vigor strong to the growth of hericium mycelium;

3. save the resource such as manpower, coal, decrease environmental pollution, meet low-carbon environment-friendly theory.

Embodiment

Below in conjunction with embodiment, the invention will be further described, and what do not illustrate in detail in embodiment is all state of the art.

Embodiment 1: a kind of making method of Hericium erinaceus culture material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: bagasse 49%, wood chip 30%, soya-bean cake 16%, mushroom tang 3.5%, potassium primary phosphate 0.1%, calcium superphosphate 0.4%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in bagasse, wood chip, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keep 24h, then turning is carried out, and turning every day later once, during first time turning, add gypsum, during second time turning, add calcium superphosphate, potassium primary phosphate, turning 3 times altogether, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, and bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 24d, mycelia covers with pocket, enters management of producing mushroom routinely.

Embodiment 2: a kind of making method of Hericium erinaceus culture material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, concrete operation step:

1. prepare burden: bagasse 49%, wood chip 30%, soya-bean cake 16%, mushroom tang 3.5%, potassium primary phosphate 0.1%, calcium superphosphate 0.4%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then the leach liquor after removing slag is admixed in bagasse, wood chip, soya-bean cake compound uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

3. turning: after material temperature reaches 60 DEG C, keep 24h, then turning is carried out, and turning every day later once, during first time turning, add gypsum, during second time turning, add calcium superphosphate, potassium primary phosphate, turning 3 times altogether, time remaining 5d, when stopping fermentation, the water content of planting material is 63%-65%;

4. pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, and bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 26d, mycelia covers with pocket, enters management of producing mushroom routinely.

Control group: Hericium erinaceus (Bull. Ex Fr.) Pers. grog is cultivated, concrete operation step:

1. prepare burden: bagasse 50.5%, wood chip 31%, soya-bean cake 17%, potassium primary phosphate 0.1%, calcium superphosphate 0.4%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%;

2. spice: first bagasse, wood chip, soya-bean cake, gypsum, calcium superphosphate siccative are mixed thoroughly, then add potassium dihydrogen phosphate aqueous solution, then add water and stir, make the water content of planting material remain on 63%-65%;

3. pack, sterilizing: employing specification is the high-pressure polypropylene plastics bag charging of 17cm × 35cm × 0.005cm, carries out normal-pressure sterilization after pack, when kettle temperature reaches 100 DEG C, keeps 10h-12h;

5. cool, inoculate: naturally cool to less than 25 DEG C and access bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 22 DEG C-24 DEG C, and humid control is at 60%-75%, and through the cultivation of 27d, mycelia covers with pocket, enters management of producing mushroom routinely.

Table one Hericium erinaceus (Bull. Ex Fr.) Pers. biological efficiency compares

As can be seen from Table I: the ferment effect utilizing mushroom tang leach liquor to add than single leach liquor together with the ferment effect that champignon stems slag adds is good, mycelial growth rate is fast, and bacterium bag pollution rate is low, and biological efficiency is high; Compared with control group, embodiment 1 all has obvious advantage in mycelial growth rate, anti-living contaminants and output.

Claims (1)

1. a making method for Hericium erinaceus culture material, comprises batching, fermentation, turning, pack, inoculation, sends out bacterium, it is characterized in that:

(1) prepare burden: bagasse 46-52%, wood chip 28-32%, soya-bean cake 14-18%, mushroom tang 3-4%, potassium primary phosphate 0.1%, calcium superphosphate 0.4%, gypsum 1%, be all the quality of dry-matter, proportioning sum is 100%; Described bagasse powder is broken into the particle of particle diameter 3mm-5mm; Described wood chip is the deciduous tree weed tree sawdust of particle diameter 3mm-5mm; Described mushroom tang is the tankage in the Lentnus edodes course of processing, is ground into the particle of 2mm-3mm after drying;

(2) ferment: mushroom tang particle is added clear water by 20-30 weight ratio doubly, soak 18h-24h at normal temperatures, then leach liquor is admixed in bagasse, wood chip, soya-bean cake compound together with champignon stems slag uniformly, stockpile is become high 1.0m-1.2m, wide 2m-3m, the trapezoidal heap that length is not limit, the culture material often piled controls at dry weight 300kg-500kg, after carrying out heap, surrounding is patted, on heap, insert some vertical pores with the wooden stick of diameter 10cm-15cm again, at the bottom of straight through reactor, stomatal frequency is 1/m ³, finally cover straw mat by its spontaneous fermentation;

(3) turning: after material temperature reaches 60 DEG C, keep 24h, then turning is carried out, and turning every day later once, during first time turning, add gypsum, during second time turning, add calcium superphosphate, potassium primary phosphate, turning 3-4 time altogether, time remaining 5d-6d, when stopping fermentation, the water content of planting material is 63%-65%;

(4) pack, inoculate, send out bacterium: the planting material fermented is after heat radiation, load the polypropylene plastics pocket of 17cm × 35cm × 0.004cm, stamp and plant bacterium hole, and bag two ends and plant in bacterium hole and put into bacterial classification, then cultivating bag is emitted in dark bacteria room, temperature controls at 18 DEG C-20 DEG C, humid control is at 60%-75%, through the cultivation of 24-26d, mycelia covers with pocket, enters management of producing mushroom routinely.