CN103109876B - Compound Chinese herbal medicament preparation for controlling cotton verticillium wilt and production method thereof - Google Patents
Compound Chinese herbal medicament preparation for controlling cotton verticillium wilt and production method thereof Download PDFInfo
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- CN103109876B CN103109876B CN201310045556.8A CN201310045556A CN103109876B CN 103109876 B CN103109876 B CN 103109876B CN 201310045556 A CN201310045556 A CN 201310045556A CN 103109876 B CN103109876 B CN 103109876B
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Abstract
The invention discloses a compound Chinese herbal medicament preparation for controlling cotton verticillium wilt and a production method thereof, and belongs to the field of Chinese herbal medicament agricultural technologies. The compound Chinese herbal medicament preparation is prepared from the following raw materials in parts by weight: 10 to 40 parts of radix astragali, 10 to 20 parts of sophora flavescens, 10 to 20 parts of coptis root, 10 to 20 parts of baikal skullcap root, 5 to 11 parts of dandelion, 5 to 10 parts of radix bupleuri, 6 to 11 parts of fructus forsythiae, 10 to 15 parts of honeysuckle, 7 to 12.5 parts of dyers woad leaf and 5 to 10 parts of wrinkled gianthyssop herb. The compound Chinese herbal medicament preparation is prepared by the steps of decocting the raw materials, filtering, and performing vacuum concentration and sterile filing. The ingredients in the compound preparation have different roles as a monarch drug, a ministerial drug, an adjuvant drug and a conductant drug, prevention and treatment are combined together, and the drug effects are synergetic, so that the cotton immunity is enhanced, the effect of killing cotton verticillium wilt viruses is outstanding, and the preparation is low in production cost and long in drug effect without residues, and has no toxic or side effect on cotton and environment.
Description
Technical field
The present invention relates to a kind of Chinese herbal formula and production method thereof of preventing and treating cotton verticillium wilt, belong to Chinese herbal medicine agricultural technology field.
Background technology
Cotton verticillium wilt (Verticillium Wilt) is a kind of soil-borne fungus venereal disease evil, it has a very wide distribution, causes harm, and degree is heavy, host range is wide, route of transmission is many, the time-to-live is of a specified duration, the cotton main producing regions such as the U.S., China, Russia, India have been spread all at present, become the first disease of the worldwide cotton of causing harm, and on producing, do not find desirable control method and Prevention Technique always, be known as the cancer of cotton.Cotton in China verticillium wilt was introduced a fine variety and is imported into from the U.S. in nineteen thirty-five, after this constantly spread, especially since the nineties in 20th century, due to reasons such as changes in environmental conditions, Insect Resistant Cotton spread, cotton region continuous cropping for many years, germ pathogenicity are changeable, verticillium wilt presents the trend that increases the weight of harm in Cotton in China main producing region.At present, China's verticillium wilt generation area accounts for plants 50% of the cotton gross area, loses every year gined cotton 7.5 * 10
4～10 * 10
4t, direct economic loss 16 * 10
8～20 * 10
8unit, has become the bottleneck that restricting current Cotton Production realizes high yield, stable yields, high-quality.
The pathogenic bacteria of cotton verticillium wilt (Verticillium Wilt) are verticillium dahliae (Verticillium dahliae), belong to Deuteromycotina (Deuteromycotina), light color Cordycepps (Moniliaceae), Verticillium dahliae genus (Verticillium).Symptoms after cotton is susceptible is varied, wherein with vascular bundle brown stain and blade wilting, comes off the most outstanding.Generally, in morbidity in the squaring period of growth rate of cotton plant, flowering and boll-setting period is onset peak, and during morbidity, leaf symptom shows as at first and between edge or master pulse, presents faint yellow irregular patch; Scab expands gradually subsequently, and overstrike, withered, upwards development gradually, and the flavescence of susceptible cotton plant blade, withered coming off, cotton boll diminishes, and de-bell rate is high, causes yield reducation.U.S. Schnathorst etc. (1996) to the pathogenic order of severity of cotton and symptom type, are divided into defoliation (T1) and non-defoliation (SS4) according to different fungus strains.Domestic to take sea-island cotton, upland cotton and the difference anti-sense kind of middle cotton 3 large cotton seeds be differential host, according to pathogenicity power, Cotton in China verticillium wilt pathogen is divided into 3 bions (Lu Jiayun, 1983).About the mechanism of causing a disease of verticillium wilt pathogen, maximum to stop up with poisoning discussion.Cotton diseased plant conduit is often because of the amount reproduction of mycelia and spore, stimulates contiguous parenchyma cell to produce colloid substance and tylosis and obstruction conduit makes the transportation of moisture and nutrient that difficulty occur simultaneously, thereby causes cotton plant to be wilted.Li Zhengli etc. had once carried out anatomic observation to cotton verticillium wilt leaf, found the phenomenon that all has mycelia, jelly and tylosis to stop up in arteries and veins base portion, middle part and top duct in disease leaf.Also someone points out, the normal potential carrying capacity of conduit is considerably beyond cotton plant gross water requirement, and therefore, conduit stops up the partly cause that just causes cotton to be wilted, and the more important thing is that germ produces the result of detoxifying function in cotton plant body.The toxic substance that the analytical proof germs such as Lv Jindian produce is a kind of acidoglycoprotein, and wherein protein content 85.25%, sugared content 14.74%.In protein, acidic amino acid accounts for 18.4%, and basic amino acid accounts for 8.3%.The toxin glycoprotein of purifying has strong the causing of the cotton seedling effect of withering.
In cotton culture kind, most sea-island cotton kinds have high disease resistance to verticillium wilt, and its disease resistance has very high stable performance between different Ji Nian borders, region, but the genetic breeding through decades facts have proved, is difficult to obtain the Upland Cotton of high resistance and high yield with classical genetic and breeding method.Prevent and treat the conventional alternating method of verticillium wilt abroad, China is difficult to application because per capita cultivated land is few.Chemical control contaminated environment, biological control cost is high and control efficiency is undesirable.
Summary of the invention
The deficiency existing for solving existing control cotton verticillium wilt technology, the invention provides a kind of Chinese herbal formula and production method thereof of preventing and treating cotton verticillium wilt, this Chinese herbal formula bactericidal properties is strong, drug effect is long, cost is low, noresidue, and long-term use is not developed immunity to drugs, to plant without injury, to person poultry safety, free from environmental pollution.
The present invention solves this technical problem adopted technical scheme: a kind of Chinese herbal formula of preventing and treating cotton verticillium wilt, it is characterized in that, by the raw material of following weight portion, made: the Radix Astragali 10～40, kuh-seng 10～20, the coptis 10～20, the root of large-flowered skullcap 10～20, dandelion 5～11, radix bupleuri 5～10, the capsule of weeping forsythia 6～11, honeysuckle 10～15, folium isatidis 7～12.5, wrinkled giant hyssop 5～10.
Production method, comprises the steps:
(1) boil: by above-mentioned weight portion, take raw material for standby; First water (amount of water is 2.5-3.5 times of Chinese medicine gross mass) is added in reactor, add the Radix Astragali, kuh-seng, the root of large-flowered skullcap, radix bupleuri and folium isatidis, heating gradually, 100 ℃～110 ℃ temperature controls react 240 ± 5 minutes, add again the coptis, the capsule of weeping forsythia, dandelion and wrinkled giant hyssop, 100 ℃～110 ℃ temperature controls react 90 ± 5 minutes, finally add honeysuckle, and 100 ℃～110 ℃ temperature controls react 50 ± 5 minutes;
(2) filter: from reactor, get rid of and boil liquid, by flame filter press, compress filtration, obtain filtered fluid;
(3) Vacuum Concentration: filtered fluid is got in spherical vacuum concentration pan by pump, temperature 100-105 ℃, vacuum is to concentrate 15～30 minutes under 620～660mmHg, obtains concentrate (concentrate of 1 kilogram of Chinese medicine material simmer down to 450-550ml), and opens outlet;
(4) sterile filling: concentrate is measured and packed into pail pack or packaging bag by liquid filling machine.
The major function of reactor of the present invention is to carry out boiling and concentration response of raw material, reactor requires sealing, without leaking, high temperature resistant, high pressure, corrosion-resistant, speed of agitator is medium.The key technical indexes: volume 1000～10000L, working pressure≤10.0Mpa, temperature≤300 ℃, speed of agitator 20～500r/min is adjustable, mode of heating: electricity, steam, conduction oil, main material is corrosion-resistant.Workshop reaches GMP requirement, and packing workshop reaches 1,000,000 grades of aseptic requirements.
The invention has the beneficial effects as follows:
1, production method of the present invention is carried out in reactor, can reach accurate temperature controlling, pressure control, and mixing of materials is even, sufficient reacting, and contained active component boils, concentrates thoroughly, has greatly improved drug effect.
2, in the present invention formula, contain 10 kinds of the Radix Astragali, kuh-seng and the coptiss etc. strengthen cotton immunity, to the outstanding Chinese medicine of cotton verticillium wilt toxic effect.Wherein the Radix Astragali, kuh-seng are monarch drug in a prescription, and the Radix Astragali mainly increases cotton immunity, and kuh-seng is mainly killed cotton verticillium wilt fungi, and all the other 6 ministerial drugs increase immunity and combine with antibacterial, monarch, and prevention and treatment combine, whole control cotton verticillium wilt.
3, the flavones in preparation of the present invention (mainly from the Radix Astragali), matrine (content >0.1%, mainly from kuh-seng), berberine (content >1%, mainly from the coptis) isoreactivity material composition, significantly strengthen cotton immunity and poisoning verticillium wilt effect.
4, this preparation effective drug duration length, noresidue, have no side effect to cotton and environment.
5, production cost is low.The Chinese herbal medicine using in this formula, is all domestic leading traditional Chinese medicines, and low price is easily purchased, and investment of production equipment, power consumption are all lower.
Type testing situation is:
The Jinxiang in three cotton regions, southwest in Shandong Province, large main cotton region in Shandong Province, the Dongying in the Linqing in western Shandong cotton region and northern Shandong cotton region, the existing kind Shandong cotton of take is respectively ground No. 28 as test kind, 2 kilograms of every mu of sowing quantities, use this compound preparation 10ml seed soaking, the 10ml pure water of take seed soaking is control group, other fertilising is identical with control measures, at three large main cotton region control group cotton in seedling stage, the flower bud phase, the term of opening bolls, verticillium wilt referred to be respectively 7.1, 15.6, 21.2, use this compound preparation seed soaking, seedling stage, the flower bud phase, the term of opening bolls, verticillium wilt referred to be respectively 0, 1.7, 3.2, reach utmost point significance level.Jinxiang, Linqing and Dongying cotton control group seed cotton yield are 278.8 kilograms, 262.5 kilograms and 227.3 kilograms, seed soaking unginned cotton per mu yield is respectively 346.5 kilograms, 315.5 kilograms and 254.4 kilograms, visible, seed soaking is respectively than control group volume increase 24.3%, 20.2% and 11.9%.
Embodiment
Embodiment 1
Formula (weight portion): the Radix Astragali 40, kuh-seng 10, the coptis 10, the root of large-flowered skullcap 10, dandelion 5, radix bupleuri 5, the capsule of weeping forsythia 6, honeysuckle 10, folium isatidis 7, wrinkled giant hyssop 5.
This immunity of filling a prescription to strengthen cotton is main, comprises the steps:
(1) boil: by above-mentioned weight portion, take raw material for standby; First water (amount of water is 3 times of Chinese medicine gross mass) is added in reactor, add the Radix Astragali, kuh-seng, the root of large-flowered skullcap, radix bupleuri and folium isatidis, heating gradually, 100 ℃～110 ℃ temperature controls react 240 ± 5 minutes, add again the coptis, the capsule of weeping forsythia, dandelion and wrinkled giant hyssop, 100 ℃～110 ℃ temperature controls react 90 ± 5 minutes, finally add honeysuckle, and 100 ℃～110 ℃ temperature controls react 50 ± 5 minutes;
(2) filter: from reactor, get rid of and boil liquid, by flame filter press, compress filtration, obtain filtered fluid;
(3) Vacuum Concentration: filtered fluid is got to after spherical vacuum concentration pan (V=500L) by pump, at 100-105 ℃, spherical vacuum concentration pan steam pressure is controlled at 0.05-0.1 Mpa, vacuum is 620～660mmHg, concentrated 15～30 minutes, obtain concentrate (1 kilogram of concentrate that traditional Chinese drug inspissation is 450-550ml) and open outlet;
(4) sterile filling: concentrate is measured and packed into pail pack or packaging bag by liquid filling machine.
Embodiment 2
Formula (weight portion): kuh-seng 20, the coptis 20, the Radix Astragali 10, the root of large-flowered skullcap 15, dandelion 11, radix bupleuri 10, the capsule of weeping forsythia 11, honeysuckle 15, folium isatidis 12.5, wrinkled giant hyssop 10.
The present invention is a kind of take poisoning cotton verticillium wilt as main formula, comprises the steps:
(1) boil: by above-mentioned weight portion, take raw material for standby; First water is added in reactor, add the Radix Astragali, kuh-seng, radix bupleuri and folium isatidis, heating gradually, 100 ℃～110 ℃ temperature controls react 240 ± 5 minutes, add again the coptis, the capsule of weeping forsythia and dandelion, 100 ℃～110 ℃ temperature controls react 90 ± 5 minutes, finally add honeysuckle, and 100 ℃～110 ℃ temperature controls react 50 ± 5 minutes;
(2) filter: from reactor, get rid of and boil liquid, by flame filter press, compress filtration, must fall filtered fluid;
(3) Vacuum Concentration: filtered fluid is got to after spherical vacuum concentration pan (V=500L) by pump, at 100-105 ℃ spherical vacuum concentration pan steam pressure to be controlled at 0.05-0.1 Mpa(vacuum be 620～660mmHg) 15～30 minutes, obtain concentrate (1 kilogram of concentrate that traditional Chinese drug inspissation is 450-550ml) and open outlet;
(4) sterile filling: concentrate is measured and packed into pail pack or packaging bag by liquid filling machine.
Claims (3)
1. a Chinese herbal formula of preventing and treating cotton verticillium wilt, it is characterized in that, by the raw material of following weight portion, made: the Radix Astragali 10～40, kuh-seng 10～20, the coptis 10～20, the root of large-flowered skullcap 10～20, dandelion 5～11, radix bupleuri 5～10, the capsule of weeping forsythia 6～11, honeysuckle 10～15, folium isatidis 7～12.5, wrinkled giant hyssop 5～10.
2. the production method of Chinese herbal formula claimed in claim 1, is characterized in that,
(1) boil: by weight portion claimed in claim 1, take raw material for standby; First water is added in reactor, add the Radix Astragali, kuh-seng, the root of large-flowered skullcap, radix bupleuri and folium isatidis, heating gradually, 100 ℃～110 ℃ temperature controls react 240 ± 5 minutes, add again the coptis, the capsule of weeping forsythia, dandelion and wrinkled giant hyssop, 100 ℃～110 ℃ temperature controls react 90 ± 5 minutes, finally add honeysuckle, and 100 ℃～110 ℃ temperature controls react 50 ± 5 minutes;
(2) filter: from reactor, get rid of and boil liquid, by flame filter press, compress filtration, obtain filtered fluid;
(3) Vacuum Concentration: filtered fluid is got in spherical vacuum concentration pan by pump, temperature 100-105 ℃, vacuum is to concentrate 15～30 minutes under 620～660mmHg, obtains concentrate, and opens outlet;
(4) sterile filling: concentrate is measured and packed into pail pack or packaging bag by liquid filling machine.
3. the production method of Chinese herbal formula as claimed in claim 2, is characterized in that, the amount of water of described step (1) is 2.5-3.5 times of Chinese medicine material gross mass.
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| CN103349014B (en) * | 2013-06-24 | 2014-12-17 | 武汉富强科技发展有限责任公司 | Compound traditional Chinese medicine for preventing and curing crop diseases and preparation method thereof |
| CN103396256B (en) * | 2013-08-16 | 2015-09-30 | 诸卫平 | The application of Leaf of Indigowoad decoction liquor in the chemical fertilizer and agricultural chemicals of preparation farm crop |
| CN104761345A (en) * | 2015-03-23 | 2015-07-08 | 戴威农业科技发展股份有限公司 | Charcoal-based functional fertilizer capable of preventing cotton continuous cropping disease and preparation method thereof |
| CN106035422A (en) * | 2016-06-22 | 2016-10-26 | 固镇玉鹏生态农业文化园有限公司 | Strawberry greensickness preventing and treating agent |
| CN106490033A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of composite pesticide of preventing and treating cotton diseases and insect pestses and preparation method thereof |
| CN112056330A (en) * | 2020-08-17 | 2020-12-11 | 石河子大学 | Cotton verticillium wilt prevention and treatment method |
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| KR20060106958A (en) * | 2005-04-07 | 2006-10-12 | (주)한국바이오케미칼 | The anti-plant pathogen composition containing extract of natural products |
| CN100577014C (en) * | 2005-10-14 | 2010-01-06 | 中国医学科学院药用植物研究所 | Plant resource disinfectants for agricultural use an preparation method thereof |
| CN101209062A (en) * | 2006-12-30 | 2008-07-02 | 中国医学科学院药用植物研究所 | Compound plant source agricultural fungicide and preparation thereof |
| CN102870833A (en) * | 2012-10-15 | 2013-01-16 | 青岛青大海魁生物技术有限公司 | Chinese medicinal herb marine polypeptide chelate fungicide and preparation method |
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Owner name: JI'NAN FEITIAN AGRICULTURAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: MENG QINGHUA Effective date: 20140409 |
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Effective date of registration: 20140409 Address after: 250100 No. 194 industrial North Road, Licheng District, Shandong, Ji'nan Patentee after: JINAN FEITIAN AGRICULTURAL SCIENCE AND TECHNOLOGY CO., LTD. Address before: 250100 science and technology industry department, Ji'nan Academy of Agricultural Sciences, 202 industrial North Road, Licheng District, Shandong, Shandong Patentee before: Meng Qinghua |
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Effective date of registration: 20201119 Address after: Room 1, floor 5, unit 4, building B, No. 70, Hongqi Street, Xiangfang District, Harbin City, Heilongjiang Province Patentee after: Gai Yanchun Address before: 250100 No. 194 industrial North Road, Licheng District, Shandong, Ji'nan Patentee before: JINAN FEITIAN AGRICULTURAL SCIENCE AND TECHNOLOGY Co.,Ltd. |
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