CN108633920A - A kind of improvement medicament of prevention water rice brown spot - Google Patents
A kind of improvement medicament of prevention water rice brown spot Download PDFInfo
- Publication number
- CN108633920A CN108633920A CN201810648966.4A CN201810648966A CN108633920A CN 108633920 A CN108633920 A CN 108633920A CN 201810648966 A CN201810648966 A CN 201810648966A CN 108633920 A CN108633920 A CN 108633920A
- Authority
- CN
- China
- Prior art keywords
- parts
- brown spot
- volume
- medicament
- rice brown
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Virology (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of improvement medicament of prevention water rice brown spot, the formula of the medicament is made of a certain proportion of bacillus subtilis, penicillium oxalicum aqua, long root gold thread fermented liquid, castor bean water body object, Halomine, sweet wormwood extracting solution, sulfonate, ethylene polyethenoxy ether, imidazoline, bleeding agent.The generation of water rice brown spot can effectively be prevented, it can also be used to the rice that treatment has been fallen ill, it is efficient up to 95.5%.The wetting agent combination that the present invention is compounded by a certain percentage using sulfonate, ethylene polyethenoxy ether, imidazoline, with preferable surface-active, compatibility and wettability, pesticide is significantly improved in the adsorption capacity and penetration on blade face, enhances drug effect, to efficiently prevent water rice brown spot.
Description
Technical field
The present invention relates to technical field of agriculture science, more particularly to a kind of improvement medicament of prevention water rice brown spot.
Background technology
Water rice brown spot, also known as Hemintho-sporum leaf spot of rice plants, flax leaf blight, be by Deuteromycotina rice Bipolaris,
Also known as rice is from a kind of fungal disease caused by compacted spore (Bipolaris oryzae).In 20th century, water rice brown spot is in Billy
When, India, Bangladesh, Iran, the country such as Philippine and South America have generation.Especially in 1945, Bangladesh of India is because recklessly
Sesame slices pinta is big to cause rice large area to lose receipts, leads to the big famine of more than 200 ten thousand people hungry to death.Meanwhile the Hubei in China,
Multiple provinces, municipalities and autonomous regions such as Jilin, Jiangsu, Jiangxi, Zhejiang, Fujian, Sichuan, Beijing, Shanghai, Ningxia, Guangxi it has also been found that this
Disease.Since 21 century, in Tianjin, Hebei Tang, Qinhuangdao, Zhaoping, Tanghai, Guangxi, cities and counties' disease such as peaceful, Zhanyi, Yunnan is again
Further occur, the degree that causes harm is rare for nearly 20 years, becomes the Major Diseases of some regional late rices.These susceptible fields in area
Block, the general underproduction 10~30%, 50% or more severe patient's underproduction are even had no harvest, and serious economic loss is caused to peasant.
Biological control is to prevent agricultural pest using beneficial living body biological itself or organism metabolite
Method.Biological control have the characteristics that safely, effectively, it is lasting, avoid the series of negative effect that chemical prevention is brought.It utilizes
Microbial metabolism liquid is as a kind of important method that medicament is also biological control.Microbial metabolism liquid is as a kind of prophylactic measures
Reference can trace back to discovery of the Pasteur in 1877 about microorganism antagonism, successively by streptomysin, tetracycline, kill swine fever
Plain S etc. is disease control for farming, then develops kasugarnycin, the non-harmful plant disease of Polyoxin lamp high-efficiency low-toxicity
Pesticide control.Microbial bactericide agriculture chemical registration is registered during China has 17 at present, and it is nuisanceless that microbial pesticide becomes research
The important directions of pesticide.
Plant surface layer majority is made of the cornea of the content of wax, these substances constitute low-energy surface, and most pesticides often with
Aqueous solution form sprays.Since the surface tension of liquid is higher than the critical surface tension in this low energy face, in general, medicine
What liquid and plant were not easy to sprawl when being in direct contact, addition wetting agent can not only increase the wetting action of liquid and crop, also protect
The effective concentration for holding pesticide enhances the absorption of plant, to improve the important function of drug effect.
Chinese patent CN201510373996 discloses that a kind of the present invention relates to a kind of prevention sides of water rice brown spot
Method includes the following steps：1) ecological management：Carry out microorganism fertilizer using fertile beneficial more or ground good fortune, improves soil physical and chemical property, improve soil
Earth fertility enhances the absorbability of rice root；2) cultural control；3) BIOLOGICAL CONTROL；4) biological control；5) physical control；6)
Chemical prevention：Medicament selects 72.2% propamocarb aqua, 600 times of liquid, 58% 500 times of liquid of first frost mancozeb wettable powder
Or 80% mancozeb wettable powder, 500 times of liquid, and match and apply 70% Wettable propineb powder, 500 times of liquid foliage-sprays；It can
Effectively to prevent water rice brown spot, while integrated control carried out using measures such as cultural control, physical controls to make rice recklessly
Sesame slices pinta average attack rate reduces, and yield increases, and significantly improves rice grade, obtains good economic benefit.
Chinese patent CN201510562533 discloses a kind of Rice Resistance flax leaf spot breeding method, the breeding method
Steps are as follows：(1) the anti-source of japonica rice material and water rice brown spot long-grained nonglutinous rice to be improved is selected；(2) to the japonica rice to be improved of selection
Material carries out the identification of Raw toxin patience concentration；(3) hybridization obtains three and hands over F1 cenospecies；(4) three friendship F1 cenospecies are carried out again
Arrange Xian culture medium Anther Cultures；(5) bloom control single plant sowing obtains flower training family, further economical character identification, flax tikka
Sick resistance screening obtains Keng rice lines.The breeding method of the present invention can be high by flax leaf spot genetics of resistance substance in long-grained nonglutinous rice
It imitates and exclusively penetrates into japonica rice, Rice Resistance flax leaf spot breeding can be completed within 3 years or so, it is disease-resistant to greatly shorten flax leaf spot
There is breeding cycle larger Breeding Application to be worth.
Currently, the control method of water rice brown spot mainly has screening to cultivate disease-resistant variety and Agro-chemicals control.Though
Right disease-resistant variety has water rice brown spot in production certain control and alleviation, but the narrow of germ plasm resource does not have
To solution at all and the selection and breeding period is longer, and the unification of kind is still troubling in production.Agro-chemicals control is mainly applied
The generation of the control disease such as Propineb, Mancozeb, different rice blast net missible oil, carbendazim and prevalence, but long-term use meeting pair
The direct murder by poisoning of non-target organism, and pollute the environment, cause pathogen to generate drug resistance, the drug effect for being declines, production
It is upper only to increase dose and application times, it is that pesticide dosage greatly improves, causes vicious circle, therefore find novel non-pollution
Pesticide control has become the task of top priority, and the benefit of biological control also more highlights.
Invention content
The technical problems to be solved by the invention：
The shortcomings that for the length of period present in existing prevention water rice brown spot method, drug resistance and environmental pollution,
Provide a kind of completely new biological control medicament of prevention flax leaf spot.
It is not easy to sprawl in plant surface for aqueous pesticide, adsorption capacity is weak, and the low disadvantage of permeability, the present invention passes through compounding
The addition of wetting agent solves the problems, such as this.
In order to solve the above technical problems, the present invention provides technical solution below：
A kind of improvement medicament of prevention water rice brown spot, it is as follows to improve each group distribution ratio in medicament：Bacillus subtilis
11~29 parts by volume of bacterium, 20~50 parts by volume of penicillium oxalicum aqua, long 20~40 parts by volume of root gold thread fermented liquid, castor bean water
15~25 parts by volume of extract, 2~6 parts by volume of Halomine, 4~18 parts by volume of sweet wormwood extracting solution, 0.25~1 volume of sulfonate
Part, 0.25~1 parts by volume of ethylene polyethenoxy ether, 0.25~1 parts by volume of imidazoline.
Preferably, the parts by volume of each component is as follows in a kind of improvement medicament of prevention water rice brown spot：Withered grass gemma
16 parts of bacillus, 36 parts of penicillium oxalicum aqua, long 30 parts of root gold thread fermented liquid, 20 parts of castor bean water extract, Halomine 4
Part, 11 parts of sweet wormwood extracting solution, 0.75 part of sulfonate, 0.75 part of ethylene polyethenoxy ether, 0.75 part of imidazoline.
Preferably, it is a kind of prevention water rice brown spot improvement medicament, the sulfonate, ethylene polyethenoxy ether,
The volume ratio of imidazoline is as follows：Sulfonate：Ethylene polyethenoxy ether：Imidazoline=1:1:1.
Preferably, the sulfonate is 60% lignin sulfonic acid saline solution, the molecule of the ethylene polyethenoxy ether
Amount is 4000~6000 dalton, and the imidazoline is 2- hydroxylsMethyl -2-50% aqueous solution of heptadecyl -4,5- glyoxalidine.
Preferably, a kind of improvement medicament of prevention water rice brown spot, the bacillus subtilis a concentration of 106~
1011A/mL, the penicillium oxalicum aqua a concentration of 15~20% (w/v, g/mL), the castor bean water extract a concentration of 1~
10mg/mL, a concentration of 2~8mg/mL of Halomine.
Preferably, long root gold thread fermented liquid is made as follows：By long root gold thread bacterium in potato sucrose culture medium
In 27 DEG C of shaken cultivations 16 days, take zymotic fluid to be filtered to get filtrate with G4 acid funnels under aseptic condition, 4 DEG C of preservations of bacteria-free filtrate are standby
With.
Preferably, a kind of improvement medicament of prevention water rice brown spot, sweet wormwood extracting solution are made as follows：(1)
The crushing sweet wormwood that will dry in the shade weighs 600g, binds up with gauze, and is placed in extraction cylinder；(2) 900mL ether is measured, is led from extraction cylinder
Enter 1L round-bottomed flasks, installs Soxhlet extractor；(3) 36 DEG C of constant temperature reflux 4h, siphon 5 postcooling extracting solutions of reflux to room
Temperature；(4) by after extracting solution rotary evaporation in vacuo, 4 DEG C save backup.
Preferably, a kind of improvement medicament of prevention water rice brown spot, castor bean water extract, which is prepared as follows, to be obtained
：Castor bean is broken into benevolence and stays seed, 100g is weighed after crushing, is 1 by solid-to-liquid ratio:35 are added distilled waters, boil after 2h while hot with 150
Mesh bilayer wire screen filters.Boiled under filtrate normal pressure be concentrated into it is thick.By dope in 65 DEG C of freeze-day with constant temperature, the brown cream of acquisition
Shape object is castor bean water extract.
The advantageous effect that the present invention obtains：
The preferred embodiment that the present invention uses can to the extracorporeal inhibiting rate of water rice brown spot pathogen rice Bipolaris
Up to 83%；The infection rate of water rice brown spot can be significantly reduced after liquid by spraying in advance, reduce compared with the control group 90% with
On；Liquid is sprayed to the metainfective rice of flax leaf spot, can significantly alleviate the Development process of water rice brown spot, and part
It cures, it is efficient up to 95.5%；The present invention significantly improves the surface-active of pesticide main effect component aqueous solution, makes the present invention and water
The adsorption capacity and penetration on rice blade face improve a lot, and wetting agent is to the disease-resistant synergistic effect of main effect component in medicament 10%
More than, meanwhile, the main effect component for compounding wetting agent and pesticide has good compounding compatibility, has to the drug effect promotion of medicament larger
It helps.
Specific implementation mode
Below by the description to embodiment, specific embodiments of the present invention will be described in further detail, with side
Those skilled in the art is helped to have more complete, accurate and deep understanding to the inventive concept of the present invention, technical solution.
Embodiment 1：Medicament is prepared as follows：
The parts by volume of each component is as follows：It is a kind of prevention water rice brown spot improvement medicament in each component parts by volume such as
Under：16 parts of bacillus subtilis, 36 parts of 20% penicillium oxalicum aqua, long 30 parts of root gold thread fermented liquid, castor bean water extract 20
Part, 4 parts of Halomine, 11 parts of sweet wormwood extracting solution, 60% 0.75 part of lignin sulfonic acid saline solution, ethylene polyethenoxy ether
0.75 part, 2- hydroxylsMethyl -2-0.75 part of 50% aqueous solution of heptadecyl -4,5- glyoxalidine.
Main effect farm chemical ingredients are as follows：16 parts of bacillus subtilis, 36 parts of 20% penicillium oxalicum aqua, long root gold thread bacterium fermentation
30 parts of liquid, 20 parts of castor bean water extract, 4 parts of Halomine, 11 parts of sweet wormwood extracting solution.
Preparation steps are as follows：
(1) long root gold thread fermented liquid is made as follows：By long root gold thread bacterium 27 in potato sucrose culture medium
DEG C shaken cultivation 16 days takes zymotic fluid to be filtered to get filtrate with G4 acid funnels under aseptic condition, and 4 DEG C of bacteria-free filtrate saves backup.
(2) sweet wormwood extracting solution is made as follows：(1) the crushing sweet wormwood that will dry in the shade weighs 600g, binds up with gauze, is placed in
It extracts in cylinder；(2) 900mL ether is measured, 1L round-bottomed flasks is imported from extraction cylinder, installs Soxhlet extractor；(3) 36 DEG C of perseverances
Temperature reflux 4h, siphon 5 postcooling extracting solutions of reflux to room temperature；(4) by after extracting solution rotary evaporation in vacuo, 4 DEG C save backup.
(3) castor bean water extract is prepared as follows acquisition：Castor bean is broken into benevolence and stays seed, 100g is weighed after crushing, presses
Solid-to-liquid ratio is 1:35 are added distilled water, are filtered while hot with 150 mesh bilayer wire screen after boiling 2h.Boiled under filtrate normal pressure be concentrated into it is viscous
Thick shape.By dope in 65 DEG C of freeze-day with constant temperature, the brown paste of acquisition is castor bean water extract.
(4) Bacillus subtilis strain is aseptically inoculated in nutrient agar inclined-plane, 37 DEG C of cultures are for 24 hours
Picking single bacterium colony is seeded in 5mL nutrient broth mediums afterwards, and for 24 hours, then expansion is seeded to 100mL for culture in 37 DEG C of shaking tables
In (5% soya bean) soya-bean milk, 37 DEG C of cultures for 24 hours, count up to 108/ mL is spare.
(5) accurate to weigh Halomine powder, it is spare to a concentration of 5mg/mL that aseptic double-distilled water is added.
(6) the volume parts accurate measuring each component in formula is pressed, up to liquid after mixing well.
Embodiment 2：Medicament is prepared as follows：(compounding wetting agent ratio preferred)
Main effect pesticide water liquor capacity part is as follows：A kind of body for improveing each component in medicament of prevention water rice brown spot
Product part is as follows：16 parts of bacillus subtilis, 36 parts of 20% penicillium oxalicum aqua, long 30 parts of root gold thread fermented liquid, castor bean water
20 parts of extract, 4 parts of Halomine, 11 parts of sweet wormwood extracting solution.
Be separately added into the above liquid individually or compounding wetting agent prepared, preparation method with embodiment 1, wetting agent
Ratio is shown in Table 1.
Table 1 individually or compound drug addition concentration and ratio
By prepared 1-7 medicaments, the angle of wetting on rice leaf is measured together with embodiment 1, to reflect table
Influence of the face activating agent compound proportion to main effect pesticide water solution-wet effect.Angle of wetting is surveyed using Wetness Angle Measuring Instrument
It is fixed, initial wetting angle is measured immediately after medicament is added dropwise in fresh rice leaf surface, and final angle of wetting is measured after three minutes.
Experimental result：
Table 2 compounds Wet Out of the medicament on Rice Leaf surface of different proportion wetting agent
As seen from the results in Table 2, when wetting agent ratio is 1：1：When 1, initial and final angle of wetting all reaches minimum, degree of wetting
Reach maximum, surface tension is minimum, and final angle of wetting can make liquid reach complete in 3 minutes after illustrating wetting agent compounding for 0
It sprawls, adsorption capacity reaches maximum.Regardless of being individually added into the angle of wetting of the ratio of wetting agent or other compounding wetting agents all
Higher than embodiment 1, wetting effect is not as good as embodiment 1, therefore it is preferred that wetting agent ratio is 1：1：1.
Embodiment 3：It is formulated as follows the liquid (compounding concentration of wetting agent preferred) of ingredient
Main effect pesticide water liquor capacity part is as follows：A kind of body for improveing each component in medicament of prevention water rice brown spot
Product part is as follows：16 parts of bacillus subtilis, 36 parts of 20% penicillium oxalicum aqua, long 30 parts of root gold thread fermented liquid, castor bean water
20 parts of extract, 4 parts of Halomine, 11 parts of sweet wormwood extracting solution.
The compounding wetting agent that various concentration is separately added into above-mentioned liquid is prepared, and 60% lignosulfonates are water-soluble
Liquid：Ethylene polyethenoxy ether：2- hydroxylsMethyl -2-50% aqueous solution=1 of heptadecyl -4,5- glyoxalidine:1:1 (volume ratio)
Preparation method with embodiment 1, the concentration gradient of wetting agent be added 0 in main effect pesticide water solution, 0.25,0.375,0.5,
0.625,0.875,1.0 volume parts.
By prepared medicament, the angle of wetting on rice leaf is measured together with embodiment 1, to reflect surface-active
Agent compounds influence of the concentration to main effect pesticide water solution-wet effect.Angle of wetting is measured using Wetness Angle Measuring Instrument, new
Fresh rice leaf surface measures initial wetting angle immediately after medicament is added dropwise, and final angle of wetting is measured after three minutes.
Table 3 compounds influence of the concentration of wetting agent to liquid degree of wetting
As shown in Table 3 when 0.75 parts by volume is added during each concentration of wetting agent is main effect pesticide aqueous solution, angle of wetting reaches
To minimum, degree of wetting is best.
Embodiment 4：It is formulated as follows the liquid of ingredient
The parts by volume of each component is as follows：11 parts of bacillus subtilis, 20 parts of 15% penicillium oxalicum aqua, long root gold thread bacterium hair
20 parts of zymotic fluid, 15 parts of castor bean water extract, 2 parts of Halomine, 4 parts of sweet wormwood extracting solution, 60% lignin sulfonic acid saline solution
0.75 part, 0.75 part of ethylene polyethenoxy ether, 2- hydroxylsMethyl -2-50% aqueous solution 0.75 of heptadecyl -4,5- glyoxalidine
Part.
Main effect farm chemical ingredients are as follows：11 parts of bacillus subtilis, 20 parts of 15% penicillium oxalicum aqua, long root gold thread bacterium fermentation
20 parts of liquid, 15 parts of castor bean water extract, 2 parts of Halomine, 4 parts of sweet wormwood extracting solution.
Specific preparation method is shown in embodiment 1.
Embodiment 5：It is formulated as follows the liquid of ingredient
The parts by volume of each component is as follows：29 parts of bacillus subtilis, 50 parts of 17.5% penicillium oxalicum aqua, long root gold thread bacterium
40 parts of zymotic fluid, 25 parts of castor bean water extract, 6 parts of Halomine, 18 parts of sweet wormwood extracting solution, 60% lignosulfonates are water-soluble
0.75 part of liquid, 0.75 part of ethylene polyethenoxy ether, 2- hydroxylsMethyl -2-50% aqueous solution of heptadecyl -4,5- glyoxalidine
0.75 part.
Main effect farm chemical ingredients are as follows：The parts by volume of each component is as follows：29 parts of bacillus subtilis, 17.5% penicillium oxalicum water
50 parts of agent, long 40 parts of root gold thread fermented liquid, 25 parts of castor bean water extract, 6 parts of Halomine, 18 parts of sweet wormwood extracting solution.
Specific preparation method is shown in embodiment 1.
Improvement medicament in the present invention is as follows to the In-vitro Inhibitory Effect test of flax leaf spot fungi and result
It is as follows：
(1) culture dish after sterilizing is added in prepared liquid and each 2mL of aseptic double-distilled water in Example 1,4,5 respectively
In, then 15mL PDA liquid mediums are poured into culture dish, liquid and PDA culture medium are mixed well, verification is sterile to be placed on 4
It is DEG C spare.
(2) the mixing flax leaf spot fungi that picking preserves is inoculated on PDA solid mediums, and 25 DEG C of dark culturing 3d are waited for
It grows mycelium and is transferred in PDA liquid medium after generating spore, shaken cultivation is for 24 hours under the conditions of 25 DEG C.
(3) plate to be tried in step 1 is taken, per bacterium solution 50 μ L, the 25 DEG C of dark culturing 3d in ware inoculation step 2,
3000rpm centrifuges 10min, discards supernatant, and weighs after drying mycelium to constant weight.
(4) this experiment is repeated four times.
Table 4 improves growth inhibition effect of the medicament to water rice brown spot bacterium.
By table 4 as it can be seen that the growth for being not added with mycelia in the culture medium of medicament is vigorous, mycelia weight is significantly higher than dosing culture
Base group, and the medicament inhibition of embodiment 1 is best.The addition of wetting agent is to the inhibiting effect unobvious of external mycelia, with master
It is suitable to imitate ingredient inhibition, it may be related with the external synergistic effect mechanism of wetting agent.Improvement medicament in the present invention is to water
The prevention effect of rice brown spot is tested and result is as follows：
It is as follows：
(1) after the mycelia on picking inclined-plane activates 3d on new PDA solid mediums, bacteria cake is taken to be transferred to the training of PDA liquid
It supports in base, 28 DEG C, 120r/min shaking table dark culturing 2d, it is spare that culture solution is homogenized 5min.
(2) culture another name for Sichuan Province is extensive 527 respectively, Nipponbare to tri-leaf period；The rice of different cultivars is randomly divided into 8 groups to control
It treats, negative control group sprays aseptic double-distilled water, and positive controls spray 40% different rice blast net missible oil, 250 times of liquid, is sprayed by per square meter
Spill the ratio uniform sprinkling of 100mL, the medicament prepared in experimental group sprinkling embodiment 1,4,5, by the ratio for spraying 50mL per square meter
Example uniformly sprinkling.
(3) with sprayer, by the bacteria suspension being homogenized, uniformly sprinkling is inoculated on each group rice tri-leaf period blade after 6h, often
Square meter sprays 50mL bacteria suspensions, and with freshness protection package bagging moisturizing 48h, 28 DEG C are further cultured for 7d, observe scab pattern, calculate each group rice
The incidence of flax leaf spot.
The number of blade of incidence=generation scab/total leaf number x100%
Table 5 improves prevention effect of the medicament to water rice brown spot
| Pretreatment mode | Extensive 527 incidence (%) in another name for Sichuan Province | Nipponbare incidence (%) |
| Aseptic double-distilled water | 72.3 | 89.1 |
| 40% different rice blast net missible oil | 19.8 | 22.7 |
| Embodiment 1 | 4.3 | 4.9 |
| 1 main effect component of embodiment | 13.9 | 17.2 |
| Embodiment 4 | 15.3 | 17.7 |
| 4 main effect component of embodiment | 20.3 | 22.9 |
| Embodiment 5 | 10.1 | 8.7 |
| 5 main effect component of embodiment | 15.7 | 19.1 |
By the data of table 5 as it can be seen that the incidence in rice variety another name for Sichuan Province extensive 527 is totally higher than japonica rice variety Nipponbare, embodiment
1, after 4,5 improvement medicament pretreatment, no matter long-grained nonglutinous rice or japonica rice, overall incidence is all substantially reduced, and overall prevention effect connects
Close or significantly good and chemical agent prevention group, can be very good the generation for preventing water rice brown spot, plays prevention effect.It is real
Example 1,4,5 is applied respectively compared with corresponding main effect component, incidence has different degrees of reduction, illustrates adding for wetting agent
Synergistic effect can be played to the prevention of water rice brown spot by entering.
Improvement medicament in the present invention is as follows to the therapeutic effect test of water rice brown spot and result
It is as follows：
(1) after the mycelia on picking inclined-plane activates 3d on new PDA solid mediums, bacteria cake is taken to be transferred to the training of PDA liquid
It supports in base, 28 DEG C, 120r/min shaking table dark culturing 2d, it is spare that culture solution is homogenized 5min.
(2) culture another name for Sichuan Province is extensive 527 respectively, Nipponbare to tri-leaf period, and with sprayer, by the bacteria suspension being homogenized, uniformly sprinkling connects
In kind to rice tri-leaf period blade, 50mL bacteria suspensions are sprayed per square meter, with freshness protection package bagging moisturizing 48h, 28 DEG C are further cultured for 7d, see
Examine scab pattern.
(3) infection rate for calculating each group water rice brown spot, 6 groups are equally divided by the rice of different cultivars by infection rate
It is treated, model group is not processed, and negative control group sprays aseptic double-distilled water, and positive controls spray 40% different rice blast net breast
Oily 250 times of liquid, by the ratio uniform sprinkling for spraying 100mL per square meter, the medicament prepared in experimental group sprinkling embodiment 1,4,5,
The ratio sprinkling of 50mL is sprayed in every square meter, it is primary that interval 7d sprays each group medicament by same operation again.
(4) last sprays and is further cultured for 7d for 28 DEG C after medicament, observes scab pattern, calculates each group medicament to rice flax tikka
The inhibiting rate of disease.
Inhibiting rate=(model group generates the number of blade of the number of blade-experimental group generation scab of scab)/total leaf number
X100%
Experimental result is as follows：
Table 6 improves prevention effect of the medicament to water rice brown spot
| Post processing mode | Extensive 527 inhibiting rate (%) in another name for Sichuan Province | Nipponbare inhibiting rate (%) |
| Aseptic double-distilled water | 1.9 | 1.2 |
| 40% different rice blast net missible oil | 75.9 | 81.1 |
| Embodiment 1 | 92.8 | 95.5 |
| 1 main effect component of embodiment | 77.9 | 83.7 |
| Embodiment 4 | 74.2 | 78.3 |
| 4 main effect component of embodiment | 63.9 | 66.6 |
| Embodiment 5 | 82.4 | 87.7 |
| 5 main effect component of embodiment | 73.2 | 78.8 |
The above results show that the present invention has significant inhibiting effect, maximal percentage inhibition to water rice brown spot substance
It is not less than common chemical prevention medicament up to 95.5%, and to the preventive and therapeutic action of water rice brown spot, most preferably
The more notable half for being higher than chemical agent and usage amount is chemical agent of the preventive and therapeutic effect of embodiment, shows for rice recklessly
The efficient preventive and therapeutic effect of sesame slices pinta.
After compounding a certain concentration and the wetting agent of ratio, wetting agent exists to the disease-resistant synergistic effect of main effect component in medicament
10% or more, by the promotion to the wetability of medicament, adsorption capacity and penetration, and then kill plant surface and internal disease
Substance is obviously improved drug effect.The present invention is a kind of efficient ecological medicament for being directed to water rice brown spot in summary, can be wide
The general flax leaf spot applied to various rice varieties prevents, and has broad application prospects.
Above example is merely illustrative of the invention's technical idea, and protection scope of the present invention cannot be limited with this, every
According to technological thought proposed by the present invention, any change done on the basis of technical solution each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by the prior art.
Claims (8)
1. a kind of improvement medicament of prevention water rice brown spot, which is characterized in that each group distribution ratio is as follows in improvement medicament：It is withered
11~29 parts by volume of careless bacillus, 20~50 parts by volume of penicillium oxalicum aqua, long 20~40 parts by volume of root gold thread fermented liquid,
15~25 parts by volume of castor bean water extract, 2~6 parts by volume of Halomine, 4~18 parts by volume of sweet wormwood extracting solution, sulfonate
0.25~1 parts by volume, 0.25~1 parts by volume of ethylene polyethenoxy ether, 0.25~1 parts by volume of imidazoline.
2. a kind of improvement medicament of prevention water rice brown spot according to claim 1, which is characterized in that improvement medicament
The parts by volume of middle each component is as follows：16 parts of bacillus subtilis, 36 parts of penicillium oxalicum aqua, long 30 parts of root gold thread fermented liquid,
20 parts of castor bean water extract, 4 parts of Halomine, 11 parts of sweet wormwood extracting solution, 0.75 part of sulfonate, ethylene polyethenoxy ether
0.75 part, 0.75 part of imidazoline.
3. a kind of improvement medicament of prevention water rice brown spot according to claim 1, which is characterized in that the sulfonic acid
Salt, ethylene polyethenoxy ether, the volume ratio of imidazoline are as follows：Sulfonate：Ethylene polyethenoxy ether：Imidazoline=1:1:1.
4. according to a kind of improvement medicament of prevention water rice brown spot of claim 1-3 any one of them, which is characterized in that
The sulfonate is 60% lignin sulfonic acid saline solution, and the molecular weight of the ethylene polyethenoxy ether is 4000~6000
Er Dun, the imidazoline are 2- hydroxylsMethyl -2-50% aqueous solution of heptadecyl -4,5- glyoxalidine.
5. a kind of improvement medicament of prevention water rice brown spot according to claim 1 or 2, it is characterised in that：It is described
Bacillus subtilis a concentration of 106~1011A/mL, the penicillium oxalicum aqua a concentration of 15~20% (w/v, g/mL) are described
Castor bean water extract a concentration of 1~10mg/mL, a concentration of 2~8mg/mL of Halomine.
6. a kind of improvement medicament of prevention water rice brown spot according to claim 1, which is characterized in that long root gold thread
Fermented liquid is made as follows：By long root gold thread bacterium in potato sucrose culture medium 27 DEG C of shaken cultivations 16 days, it is sterile
Under the conditions of take zymotic fluid to be filtered to get filtrate with G4 acid funnels, 4 DEG C of bacteria-free filtrate saves backup.
7. a kind of improvement medicament of prevention water rice brown spot according to claim 1, which is characterized in that sweet wormwood extracts
Liquid is made as follows：(1) the crushing sweet wormwood that will dry in the shade weighs 600g, binds up with gauze, and is placed in extraction cylinder；(2) it measures
900mL ether imports 1L round-bottomed flasks from extraction cylinder, installs Soxhlet extractor；(3) 36 DEG C of constant temperature reflux 4h, siphon are returned
5 postcooling extracting solutions are flowed to room temperature；(4) by after extracting solution rotary evaporation in vacuo, 4 DEG C save backup.
8. a kind of improvement medicament of prevention water rice brown spot according to claims 1 or 2, which is characterized in that castor
Numb seed water extract is prepared as follows acquisition：Castor bean is broken into benevolence and stays seed, 100g is weighed after crushing, is 1 by solid-to-liquid ratio:35 add
Enter distilled water, is filtered while hot with 150 mesh bilayer wire screen after boiling 2h；Boiled under filtrate normal pressure be concentrated into it is thick, by dope
It is castor bean water extract in the brown paste of 65 DEG C of freeze-day with constant temperature, acquisition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810648966.4A CN108633920A (en) | 2018-06-22 | 2018-06-22 | A kind of improvement medicament of prevention water rice brown spot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810648966.4A CN108633920A (en) | 2018-06-22 | 2018-06-22 | A kind of improvement medicament of prevention water rice brown spot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108633920A true CN108633920A (en) | 2018-10-12 |
Family
ID=63753098
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810648966.4A Pending CN108633920A (en) | 2018-06-22 | 2018-06-22 | A kind of improvement medicament of prevention water rice brown spot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108633920A (en) |
-
2018
- 2018-06-22 CN CN201810648966.4A patent/CN108633920A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104222076B (en) | A kind of Methylotrophic bacillus wettable powder and its preparation method and application | |
| CN101385468B (en) | Bacillus licheniformis agent for preventing and treating black shank and preparation method thereof | |
| CN100549158C (en) | A kind of campelyco and preparation method thereof | |
| CN105053014B (en) | The compound biocontrol fungicide and preparation method and purposes of a kind of matrix containing marine alga | |
| CN103667134B (en) | The Paenibacillus polymyxa N3-4 of one strain preventing and treating club-root and application thereof | |
| CN102177921B (en) | Compound biological control agent as well as preparation method and application thereof | |
| CN106942301B (en) | A kind of botanical pesticide preparation of prevention and control rice blast and preparation method thereof | |
| CN106490057A (en) | A kind of composite pesticide for Rice Cropping and preparation method thereof | |
| CN106259575A (en) | A kind of plant liquid extract and plant protecting liquid | |
| CN106465734A (en) | A kind of preventing and treating tobacco bacterial wilt fermentation liquid and preparation method thereof | |
| CN102021122A (en) | High-efficiency insecticidal fungus and applications thereof | |
| CN107006331A (en) | The stereo plantation method that a kind of Cranberry is interplanted with Chinese yew | |
| CN103109876B (en) | Compound Chinese herbal medicament preparation for controlling cotton verticillium wilt and production method thereof | |
| CN107114411A (en) | For the trichoderma asperellum DQ3 compound powders YBY of raising rice seedlings and application | |
| CN105028483A (en) | Compound bactericide containing bacillus subtilis BAB-1 and boscalid and application thereof | |
| CN105519582B (en) | A kind of prevention Tobacco Tomato root-knot nematode preparation and application | |
| CN107603906A (en) | A kind of preparation method and application for the bacterial strain and its preparation for preventing and treating potato wilt | |
| CN107580984A (en) | A kind of method of woaded blue non-polluted planting | |
| CN106478273A (en) | A kind of Herba Dendrobii special fertilizer and preparation method thereof | |
| CN108849985B (en) | Ternary compound biological agent and application thereof in prevention and treatment of ginger bacterial wilt | |
| CN116195605A (en) | Preparation method of turmeric rhizome extract, turmeric rhizome extract product and application of turmeric rhizome extract product in preventing and treating plant powdery mildew | |
| CN108633920A (en) | A kind of improvement medicament of prevention water rice brown spot | |
| CN107409862A (en) | A kind of method for preventing and treating Chinese prickly ash seedling root rot | |
| CN106472591A (en) | A kind of ecology insecticidal bactericide and preparation method thereof | |
| CN106834194B (en) | A kind of complex micro organism fungicide for preventing and treating rice blast and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181012 |
|
| RJ01 | Rejection of invention patent application after publication |