CN103349014B - Compound traditional Chinese medicine for preventing and curing crop diseases and preparation method thereof - Google Patents
Compound traditional Chinese medicine for preventing and curing crop diseases and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a compound traditional Chinese medicine for preventing and curing crop diseases and a preparation method thereof, solving the problems that the conventional crop diseases of greensickness and the like are difficult to prevent and cure. The compound traditional Chinese medicine comprises the following components in parts by weight: 120-150 parts of radix sophorae flavescentis, 50-70 parts of scutellaria baicalensis, 70-90 parts of fructus cnidii, 40-60 parts of fructus forsythia, 50-80 parts of golden cypress, 50-60 parts of honeysuckle, 40-60 parts of polygonum multiflorum, 50-60 parts of prepared rehmannia root and 40-60 parts of rheum officinale and is prepared in the following steps of: carrying out water adding, soaking, decoction, filtering and extraction. The compound traditional Chinese medicine can be used for effectively preventing and curing greensickness and various conventional diseases of crops, has no environment pollution and residue to the crops and the soil and is beneficial to crop growth.
Description
Technical field
The present invention relates to a kind of crops herbal mixture and preparation method thereof.
Background technology
Blight is the general designation of fusarium wilt, verticillium wilt, because fusarium wilt easily mixes generation with verticillium wilt.Blight is called as crops " cancer ", and being affects the destructive disease of most in production estimation, and they are harmed the crops by various ways such as pathogene obstruction conduit, generation toxin.Pathogene can also be passed through seed, soil, current and invalid body and propagate, and therefore spread speed is exceedingly fast, and meanwhile, this pathogene can in each breeding time of the invasion morbidity of crops, therefore be prevented and treated very difficult.As cotton spoting verticillium wilt, according to Institute of Plant Protection of the Chinese Academy of Agricultural Sciences statistics, national onset area reaches more than 4,000 ten thousand mu, annual loss gined cotton 7.5-10 ten thousand tons.Usually, old cotton region, the Yangtze river basin cotton spoting verticillium wilt incidence of disease is more than 40%, and new cotton region is more than 20%, and North china is even more serious.
At present by means of only using chemical pesticide, smelting treatment is carried out for this type of disease of crops.The strong chemical pesticide of antipathogen effect is only used to there is the problem of curing the symptoms, not the disease to crops blight, and easily there is the subsequent affect such as medicament residue, unfavorable crops normal growth in a large amount of chemical pesticide that uses, visible, development can resist the diseases of agricultural plants such as blight, can prevent the residue of pesticide such as grain, veterinary antibiotics again, and the organic drug that can have promotion crop growth concurrently is extremely urgent.
Summary of the invention
The object of the invention is to solve the problems of the technologies described above, a kind of environmentally safe is provided, to crops and soil noresidue, be conducive to effective control crops blight of crop growth, tomato early epidemic, late blight, gray mold, capsicum epidemic disease etc. had to the herbal mixture of the control crops epidemic disease of fine control efficiency simultaneously.
The present invention also provides a kind of preparation method of above-mentioned herbal mixture.
Herbal mixture of the present invention, be made up of the component of following portions by weight:
Kuh-seng 120-150, root of large-flowered skullcap 50-70, frutus cnidii 70-90, capsule of weeping forsythia 40-60, golden cypress 50-80, honeysuckle 50-60, fleece-flower root 40-60, prepared rhizome of rehmannia 50-60, rheum officinale 40-60.
Further be preferably kuh-seng 135-150, root of large-flowered skullcap 53-70, frutus cnidii 82-90, capsule of weeping forsythia 53-60, golden cypress 65-80, honeysuckle 55-60, fleece-flower root 45-60, prepared rhizome of rehmannia 53-60, rheum officinale 50-60.
Preparation method of the present invention is: by prescription dosage, kuh-seng, the root of large-flowered skullcap, frutus cnidii, the capsule of weeping forsythia, golden cypress are added prescription medicinal material total amount 7-9 times of water gaging, after soaking 20-28h, add the honeysuckle of described prescription dosage, the fleece-flower root, prepared rhizome of rehmannia, rheum officinale, decoct, boiling after keep micro-2-3h that boils, filter first time filtrate; In the medicinal material after filtration, add 4-6 times of water gaging again, continue to decoct, the micro-1-2h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain final product.
Plant relies on vascular bundle to maintain growth function.Find when studying plant and increasing thick speed, plant has the similar mankind " pulse " a contract unusual appearance of beating, and whenever fine day bright sun, when the sun just rises eastwardly, plant just starts contraction, continues up to the sundowners.To night, plant stops shrinking, and starts to expand, and can continue up to morning.Thick beating at plant this day at thin night, every day goes round and begins again, but beats each time, expands always slightly larger than contraction.So plant increases gradually and has slightly grown up like this.Vascular bundle (vascular bundle) is in the organ such as leaf and young stem of vascular plant, the fascicular texture be jointly made up of primary xylem and primary phleom, vascular bundle is interlaced with one another connection, form a kind of conducting system-skeleton of primary plant body transporting moisture, mineral salt and organic substance, and have the effect supporting plant corpus concurrently.When plant is subject to after parasite, fungi, bacterium, virus, bacterial endotoxin etc. infect, vascular structure changes, and causes the epidemic diseases such as blight.
Prescription of the present invention is that distinguished veteran doctors of TCM is based on treatment varication secret recipe handed down from one's ancestors, the new medicine formula be developed in conjunction with modern plants Immunology carries out adding and subtracting, wherein, kuh-seng is as monarch drug in a prescription, there is very strong anti-insect, anti parasitic effect, (common as echinococcus to helminth parasite, nematode etc.), protozoon class parasite is (common as Eimeria, anti-Cryptosporidium, trichomonad, plasmodium, trypanosome, giardia lamblia, Amoeba, neospora, Infection of Toxoplasma Gondii effect, blastocystis hominis etc.), acarid class parasite is (common as blood lice, itch mite, itch mite, vermiform mite etc.) there is very strong killing action, also has resisting pathogenic microbes (fungi simultaneously, bacterium, virus), Nnti-Bacterial endotoxin, anti-inflammatory, anti-fibrosis and the effect of enhancing immune system, we study discovery, it has the multiple parasite and pathogenic microorganisms that cause the diseases of agricultural plants such as blight significantly kills or inhibitory action, root of large-flowered skullcap toxicity is extremely low, is acknowledged as Chinese medicine antibiotic, and existing great mass of data introduces it very strong antimycotic, antibacterium, antiviral, Nnti-Bacterial endotoxin effect, also has the multiple pharmacologically active such as anti-inflammatory, immunological regulation, frutus cnidii belongs to attacks poison, desinsection, antipruritic, frutus cnidii is dropped in sewage and can kill wriggler, frutus cnidii also has antimycotic, antibacterium, antiviral, anti-trichomonal effect and hormone-like effect very by force, frutus cnidii all has inhibitory action to acrothesium floccosum, gypsum sample microspore bacterium, ulotrichy microspore bacterium in vitro, to the Newcastle virus of chick embryo culture, chicken embryo life 6 hours can be extended, the capsule of weeping forsythia not only has antimycotic, antibacterium, antiviral, Nnti-Bacterial endotoxin effect, also has the effect such as anti-inflammatory, Immune-enhancing effect, golden cypress not only has antimycotic Dengs pathogenic microorganism, anti-trichomonal and Immunologic Functions, and golden cypress has good killing action to wriggler (10ppm can kill 62% in 18 hours), housefly etc., honeysuckle has resisting pathogenic microbes, Nnti-Bacterial endotoxin, anti-inflammatory and strengthens the effect of crops immunity function, the fleece-flower root is clinical conventional tonic, not only have and strengthen crops immunity function, all there is inhibitory action in various degree to staphylococcus aureus, staphylococcus albus, shigella flexneri, sonne bacillus, typhoid bacillus 901, paratyphosum Bacterium from diphtheria bacillus, beta hemolytic streptococcus etc., also have certain inhibitory action to virus, also, clinical conventional tonic, has immunological regulation, the effect such as anti-oxidant to prepared rhizome of rehmannia, rheum officinale is also anti-infective Chinese medicine, has a broad antifungal spectrum, to parasites such as Amoeba, Trichomonas vaginalis, blood fluke and Leptospira, to pathogenic bacteria such as staphylococcus, hemolytic streptococcus, micrococcus gonococcus, diphtheria bacillus, typhoid bacillus, shigella dysenteriaes, the viruses such as infected by influenza, orphan virus, hepatitis B, polyovirus have well to be killed or inhibitory action, also has immunological regulation, the effect such as anti-oxidant in addition.9 kinds of traditional Chinese medicines, monarch drug in a prescription kuh-seng and the ministerial drug root of large-flowered skullcap, frutus cnidii, the capsule of weeping forsythia, golden cypress, adjutant honeysuckle, the fleece-flower root, prepared rhizome of rehmannia, rheum officinale acting in conjunction is in crops, there is desinsection, antimycotic, antibacterium, antiviral, strengthen crops immunologic function, promote crop growth, improve crops quality and increase the effects such as crop yield, control vascular structure changes, can be used for cotton, vegetables, fruit, the epidemics prevention and control such as grain crops fusarium wilt verticillium wilt, and also find by experiment tomato early epidemic, late blight, gray mold, capsicum epidemic diseases etc. also have fine control efficiency.This product characteristic is that anti parasitic, antimycotic, antibacterium, antiviral spectrum are wide, and effect is fast, and quick-acting, lastingly, avirulence, noresidue, without cross resistance.Pure Chinese medicine preparation, environmentally safe, to crops and soil noresidue, in herbal mixture of the present invention, active component complexity is various, embodies the feature of Chinese medicine Mutiple Targets, multipath performance drug effect.
The herbal mixture spraying that the present invention obtains adds filling method for root and uses, to cotton spoting verticillium wilt, capsicum wilt, the display of tomato verticillium wilt field test results, through statistical procedures, compare with chemical pesticide and clear water control group, difference has significance (P < 0.01), and the present invention also has prevention crops fusarium wilt simultaneously, promote that crop growthing development, usage amount are few, determined curative effect, environmentally friendly, advantage that increasing crop yield is increased income.
Embodiment
Embodiment 1
Kuh-seng 130, the root of large-flowered skullcap 70, frutus cnidii 85, the capsule of weeping forsythia 53, golden cypress 75, honeysuckle 50, the fleece-flower root 45, prepared rhizome of rehmannia 50, rheum officinale 45.
By prescription dosage, kuh-seng, the root of large-flowered skullcap, golden cypress, the capsule of weeping forsythia, frutus cnidii are added prescription medicinal material total amount 8 times of water gagings, after soaking 24h, add the honeysuckle of described prescription dosage, rheum officinale, the fleece-flower root, prepared rhizome of rehmannia, decoct, boiling after keep micro-2h that boils, filter first time filtrate; In the medicinal material after filtration, add 5 times of water gagings again, continue to decoct, the micro-1h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain herbal mixture 1.
Embodiment 2
Kuh-seng 120, the root of large-flowered skullcap 50, frutus cnidii 82, the capsule of weeping forsythia 40, golden cypress 65, honeysuckle 60, the fleece-flower root 40, prepared rhizome of rehmannia 53, rheum officinale 40.
By prescription dosage, kuh-seng, the root of large-flowered skullcap, golden cypress, the capsule of weeping forsythia, frutus cnidii are added prescription medicinal material total amount 7 times of water gagings, after soaking 28h, add the honeysuckle of described prescription dosage, rheum officinale, the fleece-flower root, prepared rhizome of rehmannia, decoct, boiling after keep micro-2.5h that boils, filter first time filtrate; In the medicinal material after filtration, add 4 times of water gagings again, continue to decoct, the micro-1.5h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain herbal mixture 2.
Embodiment 3
Kuh-seng 150, the root of large-flowered skullcap 53, frutus cnidii 70, the capsule of weeping forsythia 60, golden cypress 80, honeysuckle 55, the fleece-flower root 55, prepared rhizome of rehmannia 60, rheum officinale 55.
By prescription dosage, kuh-seng, the root of large-flowered skullcap, golden cypress, the capsule of weeping forsythia, frutus cnidii are added prescription medicinal material total amount 9 times of water gagings, after soaking 20h, add the honeysuckle of described prescription dosage, rheum officinale, the fleece-flower root, prepared rhizome of rehmannia, decoct, boiling after keep micro-3h that boils, filter first time filtrate; In the medicinal material after filtration, add 6 times of water gagings again, continue to decoct, the micro-2h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain herbal mixture 3.
Embodiment 4
Kuh-seng 135, the root of large-flowered skullcap 60, frutus cnidii 90, the capsule of weeping forsythia 55, golden cypress 50, honeysuckle 58, the fleece-flower root 50, prepared rhizome of rehmannia 58, rheum officinale 60.
Preparation method, with embodiment 1, obtains herbal mixture 4.
Embodiment 5
Kuh-seng 130, the root of large-flowered skullcap 55, frutus cnidii 75, the capsule of weeping forsythia 45, golden cypress 70, honeysuckle 52, the fleece-flower root 60, prepared rhizome of rehmannia 52, rheum officinale 50.
Preparation method, with embodiment 2, obtains herbal mixture 5.
Experimental example 1: Indoor antibacterial determination experiment:
The Indoor antibacterial of verticillium dahliae (Verticillium dahliae) is measured, existing that report the test is as follows.
One, materials and methods
1, reagent agent and process
(1) for test preparation
Herbal mixture 1(Fuqiang Tech Development Co., Ltd., Wuhan, also known as the spirit of bacterium double play), this medicament distilled water diluting 10,50,100,500,1000 and 5000 times, standard agent is treated to 50% carbendazim WP (production of Xinyi agricultural chemicals Co., Ltd) dilution 100 times, and blank is totally 8 process.
(2) strains tested
Verticillium dahliae (Verticillium dahliae) is separated qualification by Institute of Plant Protection of academy of agricultural sciences of Shandong Province, purified rear PDA medium expands numerous for subsequent use.
(3) assay method
Adopt mycelial growth determination method.The pastille culture medium flat plate of different diluted concentration (10,50,100,500,1000 and 5000 times) will be made into for trial-production herbal mixture 1, beat diameter 6mm bacterium block with card punch along cultured bacterial classification flat-plate bacterial colony edge subsequently, transplant in the dull and stereotyped central authorities of pastille.Chemicals treatment board joint bacterium simultaneously, is treated to contrast to connect bacterium without medicament, establishes 8 process altogether, often processes repetition 3 ware.Inoculation is placed in 23 DEG C of insulating boxs cultivates.When the colony diameter connecing bacterium contrast ware reaches 75mm, (7 days) measure the colony diameter of each process, obtain bacteriostasis rate.
Two, measurement result
Measurement result (see table 1) shows, herbal mixture 1 pair of verticillium dahliae (Verticillium dahliae) has good bacteriostasis, 10, the bacteriostasis rate of 50,100,500,1000 and 5000 times of diluted concentrations to verticillium dahliae is respectively 70.23%, 52.00%, 51.67%, 42.67%, 37.77% and 27.56%, and standard agent 50% carbendazim WP is 42.05% to the bacteriostasis rate of verticillium dahliae.Statistic analysis result shows, herbal mixture 1 dilutes 500 times, and to dilute 100 times of bacteriostasis close with 50% carbendazim, the bacteriostasis rate that herbal mixture 1 dilutes 10,50,100 times of dilution process is all extremely significantly better than standard agent and other bacteriostasis rate processed of herbal mixture 1, and difference has statistical significance (P ﹤ 0.01).
The spirit of table 1. bacterium double play is to verticillium dahliae bacteria inhibition assay
Three, evaluate
Indoor flat plate bacteria inhibition assay result shows, herbal mixture 1 pair of verticillium dahliae has good bacteriostasis.
Embodiment 2: the effect of control cotton spoting verticillium wilt
One, herbal mixture 2 solution is provided by Fuqiang Tech Development Co., Ltd., Wuhan.
Two, experiment purpose inquires into effect and the proper control time that herbal mixture 2 prevents and treats cotton spoting verticillium wilt.
Three, rich and powerful Science and Technology Ltd. of client's Agriculture in Shandong Province Room agricultural machinery popularization master station and Wuhan.
Four, agricultural technology service center of Jinxiang County of test unit is presided over.
Five, test area 20 mu; Demonstration area 5000 mu.
Six, Dian Chiwang village, Yang Shan town, test site Jinxiang County, Du Lou village, Li Shen village of Hua Yu township, Wang Pi village of Wang Pi township, Fu Miao village, Xiao Yun town, Jiao Hang village, Ji Shuo town, Dan Lou village, Si Mazhen Bai Miao village, Deng Miao village of Ma Miao township.
Seven, 1 seed soaking is processed: herbal mixture 2,25 milliliters are watered 1 kilogram, soak seed 1 kilogram, seed soaking time 16-24h.
2. the beginning of June (their early stage) 1:400 times of herbal mixture 2 dilutes liquid irrigating root and 1:500 times of herbal mixture 2 dilution sprays;
3. mid-June, 1:400 herbal mixture 2 filled with root and 1:400 herbal mixture 2 sprays;
4. at the bottom of July, early in August prevents verticillium wilt: 1:400 herbal mixture 2 sprays 1-2 time, 7 days interval times.
Eight, test and demonstration effect
The damping off of soaking seed seedling stage, anthracnose, angular leaf spot, epidemic disease obviously decline, and more than 60% plot just there is no generation.The time of origin of fusarium wilt postpones 5-7 days, and the incidence of disease reduces by 60%, and the morbidity of filling with root at the bottom of May reduces by 91%.Sprayed once before June 10, the fusarium wilt incidence of disease declines 98% again.To execute every early stage more than 2 times, the verticillium wilt incidence of disease is only about 6%.Contrast without herbal mixture 2, the incidence of disease reaches 30%-40%.To spray early in August at the bottom of July 1-2 time, the verticillium wilt incidence of disease is substantially about 3% again.
Yield result: the entanglement test site spraying herbal mixture 2, average yield per mu unginned cotton 485 jin.Contrast the average yield per mu 372 jin of not spraying, average mu volume increase unginned cotton 113 jin, mu increases income into 372.9 yuan.By spraying 5 calculating, investment mu cost 50 yuan, net income increases by 322.9 yuan.5000 mu of lands for growing field crops are investigated, all general mu volume increase unginned cotton 80-110 jin spraying herbal mixture 2, mu revenue growth 300 yuan.
Nine, use herbal mixture of the present invention except diseases prevention, also play simultaneously and regulate the effect of cotton growth, what spray the spirit of bacterium double play comes off than the reduction 10%-15% do not sprayed, and seat peach is fast, and bell heavily increases by 0.1 gram, precocious 2-3 days.
Ten, conclusion: this product is pure Chinese herbal and crude drugs preparations, nontoxic, noresidue, nuisanceless, can fill up the blank that chemical pesticide can not effect a radical cure cotton yellow fusarium wilt completely.Use this product safety effective, from small plot experiment and the large-scale demonstration of 2 years, all performance was cured the disease respond well, and increase yield significantly, can large scale application.
Using method and requirement: use herbal mixture 2 to adopt and put prevention first, curing the disease is auxiliary principle, should widely popularize seed soaking and fill with root, sprays and combine.Service time will at the initial stage of a disease, and interval 7-10 days sprays once.Period of disease, as the large rainy day, should be resprayed and execute once for second day.
Embodiment 3: control booth vegetable-tomato disease test of pesticide effectiveness is summed up
1. materials and methods
1.1 reagent agents:
Herbal mixture 3, Da Kening
1.2 test process
Test establishes herbal mixture 3 to use distilled water diluting 100,300 and 500 times respectively, and Da Kening totally four process, and each process repeats for three times, if process community is 6 row vegetables.Adopt the group arrangement of random district, Gong12Ge community, each plot area is about 30m
2.
1.3 experimental field overviews
Experimental field be located in the several representational tomato booth in Linzi District and carry out, crop growing state is even.
1.4 test period
Dispenser medicament, after seedling replanting or their early stage first use dilution 300 and 500 times of herbal mixtures 3 and Da Kening filling root once, sprayed once every 7-10 days, is used in conjunction 3 times.In late November dispenser, adopt the even dispenser of bodyguard's board sprayer.
1.5 investigation method
Adopt Z-shaped 5 label taking investigation, before spray medicine, investigate disease leaf (sick fruit) radix, within 1,5,10,20 day, investigate disease leaf (sick fruit) rate after last spray medicine, calculate control efficiency.2 results and analysis
2.1 herbal mixtures 3 are to the control efficiency of different tomato different diseases
Calculate by inquiry, the average control efficiency of herbal mixture 3 pairs of tomato early epidemics, late blight, gray mold is 82%, 85%, 71%, Da Kening is respectively 85%, 89%, 76% to its control efficiency respectively.
The field efficacy of 2.2 different dosages
Find out from result of the test, the preventive effect of herbal mixture of the present invention to booth vegetable disease all improves with the increase of dosage, herbal mixture 3 is respectively 65%, 77%, 84% in the preventive effect of 100,300 and 500 times of dilutions to early blight of tomato, being generally 67%, 73%, 86% to the preventive effect of tomato late blight, is 56%, 67%, 73% to the preventive effect of graw mold of tomato.
3 brief summaries and discussion
Result show the herbal mixture 3 pairs of tomato in plastic greenhouse early, late blight has good control efficiency, acts on suitable with Da Kening.Investigation prevention effect after 10th day all reaches the highest, and the investigation prevention effect after the 20th day but declines to some extent.Fine to the control efficiency of graw mold of tomato, can apply on producing its for tomato early, the control of late blight.
Table 2 bacterium double play miraculous cure effect experimental data table
Embodiment 4: control capsicum epidemic disease test report
One, test material
1, for studying thing: capsicum, kind is " figured stone green pepper No. three "
2, controlling object: capsicum epidemic disease
3, (1) test medicine: herbal mixture 4(Fuqiang Tech Development Co., Ltd., Wuhan)
(2) medicament is contrasted: Pu Like aqua (72.2% Propamocarb aqua, Bayer Bitterfeld GmbH crop science company produces)
4, treatment dosage:
(1) herbal mixture 4 100 times of dilutions;
(2) herbal mixture 4 300 times of dilutions;
(3) herbal mixture 4 500 times of dilutions;
(4) 100 grams every mu, Pu Like aqua;
(5) blank clear water
Two, experimental scheme and method
1, situation test site in experimental field is located in the Qian Zhuan village Zhang Qingtian greenhouse of Xue Zhuan town, Fei County, Shandong Province, the long 100m of this booth thing, span 7.5m, soil is fertile brown earth, preceding crop is summer radish, executes " western Shandong " Nitrogen, Phosphorus and Potassium 50kg before transplanting, Belgian potassium sulfate 25kg, " morning is rich " bio-fertilizer 100kg, fully become thoroughly decomposed chicken droppings excrement 4m
3.Capsicum in nursery on September 2 in 2011, October 25 transplant planting, line-spacing 37cm, spacing in the rows 31cm.
2, test region design
Test totally 5 process, often process repetition 3 times, and often processing community is 15 row capsicums, plot area 40m
2, each community randomized arrangement.
3, application method
November 20 found blight diseased plant, diluted liquid irrigating roots 10 days mornings of November 21 at 400 times of the experimental plot herbal mixture 4 adopting the anti-capsicum epidemic disease of herbal mixture 4; And in December 2, December 9, December 16 continuous 3 spray herbal mixtures 4 400 times of dilutions, adopt bodyguard's board knapsack hand sprayer during spray medicine, even spraying, accomplishes that drain spray is not resprayed.
4, investigation method
Every community adopts Z-shaped 5 sampling surveys, looks into 5 strains at often, before spray medicine, investigate disease phyllopodium number, within after last dispenser 1,5,10,20 day, investigates disease leaf (sick fruit) rate, calculates control efficiency.
5, drug effect computational methods adopt Deng Kenshi duncan's new multiple range method to carry out statistical analysis
In formula: CK
0---sick leaf (really) rate before the dispenser of blank district;
CK
1---sick leaf (really) rate after the dispenser of blank district;
PT
0---diseased plant rate before the dispenser of chemicals treatment district;
PT
1---diseased plant rate after the dispenser of chemicals treatment district.
Three, test results and analysis
1,3 times are used in conjunction from the dispenser of capsicum epidemic disease their early stage, 7 days, interval, after last dispenser 1 day, the preventive effect of 100,300 and 500 times of dilutions to capsicum epidemic disease of herbal mixture 4 is respectively 91.2%, 86.9%, 78%, contrast medicament mu Pu Like aqua 100 grams of preventive effects are 92.8%, wherein 100 times of dilutions of herbal mixture 4 and the preventive effect of Pu Like aqua are without significant difference, and the preventive effect of 300 and 500 times of dilutions is poorer than contrast medicament.
2, after medicine 5 days, it is 94% that the preventive effect of 100,300,500 liquid dilutions to capsicum epidemic disease of herbal mixture 4 is respectively 91.6%, 87.5%, 78.5% contrast medicament Pu Like aqua preventive effect, 100 times of dilution preventive effects of herbal mixture 4 and Pu Like aqua are without significant difference, and 300 and 500 times of dilution preventive effects are poorer than general aqua of making every effort to overcome.
3, after medicine 10 days, the preventive effect of 100,300,500 times of dilutions to capsicum epidemic disease of herbal mixture 4 is respectively 93.3%, 87.5%, 79.9%, contrast medicament Pu Like aqua preventive effect is 94.6%, 100 times of dilution preventive effects of herbal mixture 4 and Pu Like aqua are without significant difference, and 300 and 500 times of dilution preventive effects of herbal mixture 4 are poorer than general aqua of making every effort to overcome.
4, after medicine 20 days, the preventive effect of 100,300,500 times of dilution liquid to capsicum epidemic disease of herbal mixture 4 is respectively 95.8%, 91.2%, 82.9%, it is general that to make every effort to overcome aqua to the preventive effect of capsicum epidemic disease be 97%, 100 times of liquid of herbal mixture 4 and Pu Like aqua are without significant difference, and 300 and 500 times of dilution preventive effects of herbal mixture 4 are poorer than general aqua of making every effort to overcome.
5, duration of test observation herbal mixture 4 respectively processes and has no adverse effect to capsicum, and it is dense to use each process community capsicum of herbal mixture 4 all to show leaf color depth, and robust growth, illustrates that herbal mixture 4 can promote chili growth effect.
Table 3 bacterium double play spirit control capsicum epidemic disease result of the test table
Claims (2)
1. prevent and treat a herbal mixture for crops epidemic disease, it is characterized in that, be made up of the component of following portions by weight:
Kuh-seng 120-150, root of large-flowered skullcap 50-70, frutus cnidii 70-90, capsule of weeping forsythia 40-60, golden cypress 50-80, honeysuckle 50-60, fleece-flower root 40-60, prepared rhizome of rehmannia 50-60, rheum officinale 40-60;
First by the parts by weight of traditional Chinese medicinal components, kuh-seng, the root of large-flowered skullcap, frutus cnidii, the capsule of weeping forsythia, golden cypress are added traditional Chinese medicinal components total amount 7-9 times water gaging, after soaking 20-28h, add the honeysuckle of described traditional Chinese medicinal components parts by weight, the fleece-flower root, prepared rhizome of rehmannia, rheum officinale, decoct, boiling after keep micro-2-3h that boils, filter first time filtrate; In the medicinal material after filtration, add 4-6 times of water gaging again, continue to decoct, the micro-1-2h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain final product.
2. the herbal mixture of control crops epidemic disease as claimed in claim 1, is characterized in that, be made up of the component of following portions by weight:
Kuh-seng 135-150, root of large-flowered skullcap 53-70, frutus cnidii 82-90, capsule of weeping forsythia 53-60, golden cypress 65-80, honeysuckle 55-60, fleece-flower root 45-60, prepared rhizome of rehmannia 53-60, rheum officinale 50-60.
3. the preparation method of the herbal mixture of the control crops epidemic disease described in a claim 1 or 2, it is characterized in that, by the parts by weight of traditional Chinese medicinal components, kuh-seng, the root of large-flowered skullcap, frutus cnidii, the capsule of weeping forsythia, golden cypress are added traditional Chinese medicinal components total amount 7-9 times water gaging, after soaking 20-28h, add the honeysuckle of described traditional Chinese medicinal components parts by weight, the fleece-flower root, prepared rhizome of rehmannia, rheum officinale, decoct, boiling after keep micro-2-3h that boils, filter first time filtrate; In the medicinal material after filtration, add 4-6 times of water gaging again, continue to decoct, the micro-1-2h that boils of maintenance after boiling, filters and to obtain second time filtrate; Merge twice filtrate, be concentrated into 1000 weight portions, obtain final product.
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| CN104782676A (en) * | 2015-03-19 | 2015-07-22 | 陕西乾龙高科生态农业有限公司 | Plant protective agent and preparation method thereof |
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| CN103548907B (en) * | 2013-11-11 | 2015-07-22 | 深圳市中纺滤材无纺布有限公司 | Herbal traditional Chinese medicine antibacterial composition and traditional Chinese medicine antibacterial filter screen |
| CN104026160A (en) * | 2014-06-26 | 2014-09-10 | 太仓市荣德生物技术研究所 | Bactericide for crops |
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