CN104068020A - Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis - Google Patents
Application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis Download PDFInfo
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- CN104068020A CN104068020A CN201410332282.5A CN201410332282A CN104068020A CN 104068020 A CN104068020 A CN 104068020A CN 201410332282 A CN201410332282 A CN 201410332282A CN 104068020 A CN104068020 A CN 104068020A
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Abstract
The invention relates to application of tea saponin in prevention and treatment on soybean sporocyst nematodiasis, and belongs to the field of biological prevention and treatment. The tea saponin serves as a main effective component, is high in toxic activity to heterodera glycines, and can be effectively used for preventing and treating the soybean sporocyst nematodiasis.
Description
Technical field
The present invention relates to biological control field, particularly relate to the application of Tea Saponin (Tea saponin) control soybean cyst nematode evil.
Background technology
Plant nematode is one of plant important pathogen biology, along with farmland circulation, modern agriculture is to industrialization, baseization development, long-term establishing in large scale monoculture, and the raising of continuous cropping throughout the year and soil recycling rate of waterused, the quantity of nematode in soil is accumulated rapidly, and nematodiasis problem becomes increasingly conspicuous.Wheat cyst roundworm (the Heterodera.avenae of China, Heterodera.filipjevi), soy bean cyst roundworm (Heterodera glycines) generally occurs and endangers serious, China's agricultural production is caused to heavy losses and impact (Peng Deliang etc., 1993,2008,2012; Feng Zhixin, 2001).Cyst roundworm is the narrow parasitic nematode of a class host range; after female worm maturation, ovum great majority stay in the body; female worm epidermis thickening forms sporangiocyst with the ovum in protective; after plant results; sporangiocyst drops in soil; can in soil, survive several years more than ten years even, therefore comparing with root-knot nematode and nematodes is the nematode (Feng Zhixin, 2001) that a class is difficult to control more.Nematode killing agent is one of effective measures of preventing and treating at present nematodiasis, but current nematocide is high malicious persistent pesticide, very easily cause the pollution to crops and ecotope, and also on producing, there is pesticide resistance problem in the biological pesticide avermectin that toxicity is lower at present.Therefore seeking new biological control nematode killing agent, is one of study hotspot of preventing and treating at present crops nematodiasis.
Plant be bioactive compound natural treasure-house, directly natural products (as the complete stool of plant or certain part) is processed into preparation in order to Control pests (as rotenone, nimbin), or fully studying natural plant active insecticidal components, structure-activity relationship, on the basis of poisoning mechanism, the high-efficient low toxicity insecticide that analog synthesis is new, this has had very successful precedent aspect vegetable insecticide, in contrast to this, the research of plant resource nematode killing agent is relatively backward, until after the eighties in 20th century, the research of finding natural nematocidal active material from plant just starts to receive publicity, application number is that the Chinese invention patent of CN201210050127.5 discloses the prevent and treat method of Tea Saponin to root knot nematode, Wu Huiping, Li Lili, Wang Long equality successively reports that Tea Saponin is to pine wood nematode, Hirschmanniella Oryzae, root-knot nematode and wheat cyst roundworm have good cytotoxicity and control efficiency (Wu Hui equality, 2007, Li Lili, 2012, Wang Long equality, 2013a, 2013b), application number is that 2009101162680 denomination of invention discloses Tea Saponin to Hirschmanniella Oryzae, sweet potato stem eelworm for " tea saponin granular formulation for killing nematode and preparation method thereof " patent, Meloidogyne incognita, pine wood nematode have preventive and therapeutic effect, and to rhabditis axei without lethal activity, show that Tea Saponin not has lethal activity to all line insect types.And also there is not Tea Saponin at present to soybean cyst nematode harmful research report and patent application.
Tea Saponin (Tea saponin) is a kind of natural nonionic surface active agent, from tea seed seeds, stem and the leaf extraction of Theaceae, obtain, by hydrophilic glycosyl and hydrophobic aglycon and organic acid, formed, in 1931, by Japanese scholars green hill time Lang Shouci, extracted and obtain.Tea Saponin is a kind of pentacyclic triterpene saponin (Pentacyclic Triterpenoid), and from carbon skeleton structure, it belongs to oleanane (Oleanane) class, and by sapogenin, glycosyl and organic acid composition, average chemical molecular formula is: C
57h
90o
26.Tea Saponin has the multiple biological actions such as haemolysis, antibacterial desinsection and ichthyotoxin, in China, mainly as dispersant, suspending agent, wetting agent, emulsifier, be applied in pesticide processing at present, can greatly improve the moistening dispersibility of former medicine, reduce medical liquid surface tension, strengthen wet malleability; Application aspect washing is domestic has developed various fabric special type detergent (Zhou Hao etc., 2009; Xia Chunhua etc., 2000).
Summary of the invention
For the blank in above-mentioned field, the new application of a kind of Tea Saponin in control soybean cyst nematode evil is provided, when its concentration reaches 8mg/mL, very high to the lethal activity of soy bean cyst roundworm, can be used as agricultural chemicals and use.
The application of Tea Saponin in control soybean cyst nematode evil.
Described control, for suppressing the hatching of soy bean cyst roundworm ovum, is killed second instar larvae.
Described Tea Saponin is as the effective ingredient of agricultural chemicals.
In the present invention, take Tea Saponin as effective ingredient, add in agriculture pharmaceutical carrier or composition, make agricultural chemicals, can adopt the common formulations of pesticide and using method, for preventing and treating soybean cyst nematode evil.
The present invention experimental results show that, Tea Saponin shows soy bean cyst roundworm (Heterodera glycines) indoor biometrics, Tea Saponin with 2mg/mL, 5mg/mL and 8mg/mL concentration is processed 3d, and soy bean cyst roundworm ovum hatching inhibiting rate is respectively to 43.12%, 67.25% and 75.36%; Same concentration is processed 5d, and ovum hatching inhibiting rate is respectively 59.98%, 76.31% and 85.32%; Process 10d, ovum hatching inhibiting rate has all reached more than 71.57%.
Tea Saponin is with 2mg/mL, 5mg/mL and 8mg/mL concentration processed soybeans cyst roundworm second instar larvae 72h, and with clear water recovery 24h, corrected mortality is respectively 29.86%, 59.92% and 86.14%.
Field experiment result shows, Tea Saponin has good control efficiency to soybean cyst nematode, and (area is 15m in 90% Tea Saponin Yi Mei community
2) 120g, 240g, 300g ditch spread is processed, and control efficiency is respectively 43%, 49.9%, 65%, and the preventive effect that the first two concentration is processed is suitable with nematode killing agent lythidathion, and the preventive effect that the every community of 300g concentration is processed is 65%, is obviously better than the preventive effect of lythidathion 48.8%.
Advantage of the present invention:
1) Tea Saponin of the present invention derives from Theaceae tea seed seeds sons, stem and leaf extract and obtain, and also have a small amount of existence in tealeaves, prove safer to human body.
2) field soybean cyst roundworm disease control test shows, Tea Saponin is better than current conventional chemical nematocide lythidathion to the control efficiency of soybean cyst nematode evil.
3) Tea Saponin of the present invention derives from natural plants, is easy to degraded in environment, therefore compares with nematocide, has the characteristic of low toxicity and low residue, meets the needs of environment protective plant protecting and sustainable agriculture prodution and development.
Embodiment:
Below testing and operating is to further illustrate of the present invention, and should not regard limitation of the present invention.
The present invention's Tea Saponin used is commercially available prod, derives from tea seed seeds, stem and leaf, content 98%.
Embodiment 1: to soy bean cyst roundworm ovum, hatching suppresses determination of activity to Tea Saponin
Dissecting the picking color and luster ripe soy bean cyst roundworm more consistent with size under border, with 1% clorox sterilization 3-5min, then use aseptic water washing several times, put into 24 orifice plates, every processing adds 10 ripe soy bean cyst roundworms, add 1mL for examination Tea Saponin solution, test solution is prepared with aqua sterilisa, and 4 repetitions are established in every processing, with sterile water, contrast, put 25 ℃ of cultivations, respectively at 3d, 5d, 10d observed and recorded nematode hatching quantity, calculate as follows line eggs hatching inhibiting rate.
Result shows to process 3d with the Tea Saponin of 2mg/mL, 5mg/mL and 8mg/mL concentration, and soy bean cyst roundworm ovum hatching inhibiting rate is respectively to 43.12%, 67.25% and 75.36%; Same concentration is processed 5d, and ovum hatching inhibiting rate is respectively 59.98%, 76.31% and 85.32%; Process 10d, ovum hatching inhibiting rate has all reached more than 71.57% and (has referred to table 1).
Table 1 Tea Saponin is active to the inhibition of soy bean cyst roundworm ovum hatching
Note: 1) in table, data format is:
represent and repeat mean value 4 times, S.E is standard error;
2) in table, data are to repeat mean value 4 times, with indicating same letter after column data, are illustrated in 5% difference not remarkable (DMRT).
Embodiment 2: Tea Saponin is measured the cytotoxicity of soy bean cyst roundworm second instar larvae
Adopt liquid infusion method: the test solution that adds 1mL in 24 orifice plates, test solution is prepared with aqua sterilisa, then add the bar for examination nematode 30-50, be placed in 25 ℃ of insulating boxs, in 24h, 48h, 72h, under inverted microscope, check and record nematode borer population alive and dead borer population, by formula 3, calculating corrected mortality separately.And, by what process 72h, carry out clear water recovery 24h, again under inverted microscope, check and record nematode live borer population and borer population extremely, by formula, calculate below recovery 24h corrected mortality.4 repetitions are established in every processing, with sterile water, contrast.
Result shows, Tea Saponin is processed 72h with 2mg/mL, 5mg/mL and 8mg/mL concentration, and with clear water recovery 24h, corrected mortality is respectively 29.86%, 59.92% and 86.14%; Tea Saponin is processed respectively 24h, 48h and 72h with 5mg/mL concentration, and corrected mortality is respectively 49.83%, 63.57% and 77.35% (referring to table 2).
Table 2 Tea Saponin kills line activity to soy bean cyst roundworm second instar larvae
Note: 1) in table, data format is:
represent and repeat mean value 4 times, S.E is standard error;
2) in table, data are to repeat mean value 4 times, with indicating same letter after column data, are illustrated in 5% difference not remarkable (DMRT).
Embodiment 3:
To June, infect ground at base, Langfang, the Chinese Academy of Agricultural Sciences Institute of Plant Protection soy bean cyst roundworm in May, 2013, carried out Tea Saponin control soybean cyst nematode field district test, and community area is 15m
2.
If the every community 120g of Tea Saponin, 240g, tri-kinds of concentration of 300g are processed, and contrast medicament Wei Mei community 10% lythidathion granule 50g, establishes not dispenser blank (CK), processes 3 times for every kind and repeats, randomized arrangement.May 17, even rear half a step, the 15m of rising harrowed in ground
2yi Ge community, outputs 4 ditches in community, treatment agent is evenly spread fertilizer over the fields in ditch, then soya seeds is sown in the ditch of having executed medicament, and last earthing is also treaded, and water next day.After one month, carried out sampling survey June 20, each community is got 10 strains at random, records individual plant sporangiocyst number, and carries out statistical analysis, calculates preventive effect.Result shows, Tea Saponin Yi Mei community 120g, 240g, 300g processes, and control efficiency is respectively 43%, 49.9%, 65%, the preventive effect that the first two concentration is processed is suitable with nematode killing agent lythidathion, and the preventive effect that the every community of 300g concentration is processed is 65%, is obviously better than the preventive effect (referring to table 3) of lythidathion 48.8%.
Table 3 Tea Saponin is processed the control efficiency to soybean cyst nematode
Claims (3)
1. the application of Tea Saponin in control soybean cyst nematode evil.
2. application according to claim 1, described control is for suppressing the hatching of soy bean cyst roundworm ovum and killing second instar larvae.
3. application according to claim 1, described Tea Saponin is as the effective ingredient of agricultural chemicals.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259375A (en) * | 2016-08-20 | 2017-01-04 | 田文华 | A kind of containing tea saponin with the nematicidal composition of dazomet |
| CN113767927A (en) * | 2021-07-14 | 2021-12-10 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Imidacloprid and tea saponin compound agent and method for preventing and treating wheat diseases |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485308A (en) * | 2009-03-03 | 2009-07-22 | 安徽农业大学 | Tea saponin granular formulation for killing nematode and preparation method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485308A (en) * | 2009-03-03 | 2009-07-22 | 安徽农业大学 | Tea saponin granular formulation for killing nematode and preparation method thereof |
Non-Patent Citations (3)
| Title |
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| 吴慧平 等: "茶皂素杀线虫活性测定分析", 《植物病理学报》 * |
| 廖金玲 等: "《中国线虫学研究》", 31 July 2012 * |
| 王龙平: "茶皂素杀线虫活性和大田防效", 《中国优秀硕士学位论文全文数据库 农业科学辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259375A (en) * | 2016-08-20 | 2017-01-04 | 田文华 | A kind of containing tea saponin with the nematicidal composition of dazomet |
| CN106259375B (en) * | 2016-08-20 | 2018-10-16 | 泌阳县长旺农业科技发展有限公司 | A kind of nematicidal composition containing Tea Saponin and dazomet |
| CN113767927A (en) * | 2021-07-14 | 2021-12-10 | 广州甘蔗糖业研究所湛江甘蔗研究中心 | Imidacloprid and tea saponin compound agent and method for preventing and treating wheat diseases |
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