CN102726455A - Botanical nematocide and preparation method thereof - Google Patents
Botanical nematocide and preparation method thereof Download PDFInfo
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- CN102726455A CN102726455A CN2012102422545A CN201210242254A CN102726455A CN 102726455 A CN102726455 A CN 102726455A CN 2012102422545 A CN2012102422545 A CN 2012102422545A CN 201210242254 A CN201210242254 A CN 201210242254A CN 102726455 A CN102726455 A CN 102726455A
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Abstract
The invention discloses a botanical nematocide and a preparation method of the botanical nematocide, belonging to the biopesticide and preparation technology; the botanical nematocide comprises the following raw materials in percentage by weight: flax root ethanol extract, 99% of ethanol, polyvinyl alcohol, polyethylene glycol, methylcellulose, penetrating agent T, peregal, diatomite and water; the preparation method comprises the steps of preparing a flax root ethanol extracting solution, a film-forming solution and a bonding solution; then adding the flax root ethanol extracting solution, the film-forming solution, the bonding solution, the penetrating agent T, peregal, diatomite and water to a sanding kettle for grinding; stopping grinding when the grain size reaches the standard; stewing 30 minutes for precipitation, so as to acquire the botanical nematocide product. The botanical nematocide has a rational formula, a simple manufacturing process, low product cost and good effects, is nontoxic, harmless and residual-free, and is beneficial to improving the quality and yield of soybeans.
Description
Technical field
The invention belongs to biopesticide and technology of preparing thereof, relate generally to medicine based on herbs and preparation technology's method thereof of the special-purpose poisoning soybean SCN of soybean crops.
Background technology
Soybean SCN (soybean cyst nematode is called for short SCN) disease is the main disease of soybean, contaminates by soybean SCN (Heterodera glycines) and causes, and be that soybean produces upward one of popularity, destructive disease in the world.The area takes place quite serious in China Heilungkiang, Jilin, Liaoning, Henan, the Inner Mongol etc.The characteristics that this disease takes place be distribute wide, harm weight, host range broad, the route of transmission is many, hypopus (cyst) time-to-live is long, once infection, extremely difficulty is prevented and treated.
The prophylactico-therapeutic measures of soybean cyst nematode, present domestic employing several different methods: plant anti-disease tolerant variety, agricultural measures, biological control, chemical control.Wherein using the most extensively is chemical control, and common chemical nematode killing agent mainly contains the furans pellet, salt etc. tie up in the line agent of going out, Hostathion, first, and the chemical pesticide desinsection is quick and effective.But have side effects thereupon is permanent, and the chemical nematode killing agent in being manured into soil has changed soil physico-chemical characteristic, has influenced the edaphon ecotope, and the circulation of holding together in the soil nutritive element such as nitrogen random stays the residue that is out of favour.In addition, there is proof to show, uses chemical nematode killing agent to cause nematode that agricultural chemicals is produced resistance for a long time, can't wipe out and treat insect pests and plant diseases more.In recent years, along with improving constantly of human civilization degree, the requirement that human environmental consciousness reaches self quality of life also improves constantly, and uses medicament efficient, low toxicity to prevent and treat nematode, particularly seems more urgent in organic soy planting matrix terrestrial applications.
Summary of the invention
The object of the invention is exactly the needs to problem, defective and the production of above-mentioned prior art existence and people's life; Research and develop a kind of plant resource nematode killing agent of new prescription, reach that manufacturing approach is simple, prescription is reasonable, cheap, kill that line is effective, the purpose of nontoxic noresidue.
The objective of the invention is to realize like this: the plant resource nematode killing agent is made up of following composition by weight percentage: flax root ethanol extract 4.00%, 99% ethanol 4.95%, polyvinyl alcohol 0.20%; Polyethylene glycol 0.05%; Methylcellulose 0.50%, penetrant t 0.40%, peregal 1.40%; Diatomite 4.50%, water 84.00%.
A kind of preparation method of the nematode killing agent of plant resource as stated; 1., flax root is pulverized the back extract three times with 99% alcohol reflux its preparation method may further comprise the steps:, filtrating merges, and concentrating under reduced pressure is filtrated under 40 ℃ of conditions; Get flax root ethanol extract, subsequent use in the brown bottle of packing into; 2., in the container of splendid attire polyvinyl alcohol, add water, heat temperature raising to 75 ℃, stirring and dissolving is reduced to room temperature after the dissolving, add polyethylene glycol continue to stir dissolve fully film forming liquid, subsequent use; 3., in the container that methylcellulose is housed, add water, soaking at room temperature 24 hours, dissolve fully binding liquid, subsequent use; 4., according to 1: 1.3 material: the sand volume ratio; The abrasive body bead is at first dropped in the sand milling still; Again with step 1., 2., 3. in flax root ethanol extract, film forming liquid, binding liquid and penetrant t, peregal, diatomite and the water of preparation add in the lump in the sand milling still and grind; At any time sampling and measuring granularity when the particle of granularity≤2 micron accounts for more than 92% or the particle of granularity≤4 micron accounts for 95% when above, stops grinding operation; Static 30 minutes post precipitations get plant resource nematode killing agent product.
This plant resource nematode killing agent prescription adopts orthogonal test method; Filter out the optimum formula combination; Utilize the m-phenethyl benzonitrile, 2.4-((IE)-3-hydroxyl-1-the pi-allyl)-2-metoxyphenol that contain in flax root ethanol extract to accomplish line operation extremely for the main line composition that kills; Have prescription rationally, manufacturing process is simple, product price is cheap, kill that line is effective, the characteristics of nontoxic noresidue, helps the raising of soybean yields and quality.
Embodiment
Give an actual example below and describe the present invention.
Embodiment one:
1., take by weighing 34000 gram flax roots after crushed; Extract three times with 99% alcohol reflux; Filtrating merges; Under 40 ℃ of conditions concentrating under reduced pressure filtrate 900 gram flax root ethanol extracts, in described 900 gram flax root ethanol extracts, contain flax root ethanol extract 400 grams respectively and restrain with 99% ethanol 500, with flax root ethanol extract pack into keep in Dark Place in the brown bottle subsequent use; 2., weighing polyvinyl alcohol 20 gram packs in the container, adds water 500 grams and is heated to 75 ℃, stirring and dissolving is reduced to room temperature after the dissolving, add polyethylene glycol 5 grams and continue to stir, fully after the dissolving 525 gram film forming liquid, subsequent use; 3., in the container that methylcellulose 50 gram is housed, add water 500 grams, soaking at room temperature 24 hours, fully after the dissolving 550 gram binding liquid, subsequent use; 4., according to 1: 1.3 material, sand volume ratio the abrasive body bead is at first put in the sand milling still; Then will be 1., 2., 900 gram flax root ethanol extracts 3., 525 gram film forming liquid, 550 gram binding liquid and 40 gram penetrant ts, 140 gram peregals, 450 gram diatomite and 7395 gram water put in the lump in the sand milling still and grind; At any time sampling and measuring granularity; When the particle of granularity≤2 micron accounts for more than 92% or the particle of granularity≤4 micron accounts for 95% when above; Stop grinding operation, static 30 minutes post precipitations get plant resource nematode killing agent product.
Embodiment two:
1., take by weighing after 68000 gram flax roots pulverize; Extract three times with 99% alcohol reflux; Filtrating merges; Under 40 ℃ of conditions concentrating under reduced pressure filtrate 1800 gram flax root ethanol extracts, in described 1800 gram flax root ethanol extracts, contain flax root ethanol extract of 800 grams and 99% ethanol of 1000 grams respectively, with flax root ethanol extract pack into keep in Dark Place in the brown bottle subsequent use; 2., weighing polyvinyl alcohol 40 gram packs in the container, adds water 1000 grams, is heated to 75 ℃, stirring and dissolving is reduced to room temperature after the dissolving, add polyethylene glycol 10 grams and continue to stir, fully after the dissolving 1050 gram film forming liquid, subsequent use; 3., in methylcellulose 100 gram containers are housed, add water 1000 grams, soaking at room temperature 24 hours, fully after the dissolving 1100 gram binding liquid, subsequent use; 4., according to 1: 1.3 material, sand volume ratio the abrasive body bead is at first put in the sand milling still; Then will be 1., 2., 1800 gram flax root ethanol extracts 3., 1050 gram film forming liquid, 1100 gram binding liquid and 80 gram penetrant ts, 280 gram peregals, 900 gram diatomite and 14790 gram water put in the lump in the sand milling still and grind; At any time sampling and measuring granularity; When the particle of granularity≤2 micron accounts for more than 92% or the particle of granularity≤4 micron accounts for 95% when above; Stop grinding operation, static 30 minutes post precipitations get plant resource nematode killing agent product.
Result of the test: Toxicity Determination plant resource nematode killing agent is best to soybean packing nematode toxic effect, and new multipole difference analysis plant resource nematode killing agent is higher than to produce to soybean packing nematode toxic effect extremely significantly goes up the 2% AVM conventional dose of using.Do the potted plant test of pesticide effectiveness of soybean, every 100g cultivating soil contains 100 of soybean SCN cysts in the basin, and every basin is planted 5 strain soybean, emerges to pull out big bean seedlings in back 45 days, detects cyst number in the basin, and survey soybean plant height, overground part fresh weight, root weigh.The result is following: with plant resource nematode killing agent dressing soya seeds, in the basin in the soil cyst slip be 42%; Usefulness contrast dressing seed, cyst slip 28.9% in the soil in the basin; Seed does not process, in the basin in the soil cyst increasing rate be 17%.With plant resource nematode killing agent dressing soya seeds, the comparison of soybean plant height is according to the high 3cm of chemicals treatment in the basin, and the comparison of acrial part fresh weight increases by 19% according to chemicals treatment, the increase by 14% of root anharmonic ratio contrast chemicals treatment; With plant resource nematode killing agent dressing soya seeds, the soybean plant height is than the high 7cm that does not handle in the basin, and the acrial part fresh weight is than the increase of not handling 29%, the increase by 23% that the root anharmonic ratio is not handled.
Claims (2)
1. plant resource nematode killing agent is characterized in that said plant resource nematode killing agent is made up of following composition by weight percentage: flax root ethanol extract 4.00%, 99% ethanol 4.95%; Polyvinyl alcohol 0.20%, polyethylene glycol 0.05%, methylcellulose 0.50%; Penetrant t 0.40%; Peregal 1.40%, diatomite 4.50%, water 84.00%.
2. the preparation method of a plant resource nematode killing agent as claimed in claim 1; It is characterized in that the preparation method may further comprise the steps: 1., flax root is pulverized the back with 99% alcohol reflux extraction three times; Filtrating merges; Concentrating under reduced pressure filtrating gets flax root ethanol extract under 40 ℃ of conditions, and is subsequent use in the brown bottle of packing into; 2., in the container of splendid attire polyvinyl alcohol, add water, heat temperature raising to 75 ℃, stirring and dissolving is reduced to room temperature after the dissolving, add polyethylene glycol continue to stir dissolve fully film forming liquid, subsequent use; 3., in the container that methylcellulose is housed, add water, soaking at room temperature 24 hours, dissolve fully binding liquid, subsequent use; 4., according to 1: 1.3 material: the sand volume ratio; The abrasive body bead is at first dropped in the sand milling still; Again with step 1., 2., 3. in flax root ethanol extract, film forming liquid, binding liquid and penetrant t, peregal, diatomite and the water of preparation add in the lump in the sand milling still and grind; At any time sampling and measuring granularity when the particle of granularity≤2 micron accounts for more than 92% or the particle of granularity≤4 micron accounts for 95% when above, stops grinding operation; Static 30 minutes post precipitations get plant resource nematode killing agent product.
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| CN2012102422545A CN102726455A (en) | 2012-07-02 | 2012-07-02 | Botanical nematocide and preparation method thereof |
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| CN2012102422545A CN102726455A (en) | 2012-07-02 | 2012-07-02 | Botanical nematocide and preparation method thereof |
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| CN102726455A true CN102726455A (en) | 2012-10-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104522065A (en) * | 2015-02-03 | 2015-04-22 | 黑龙江省科学院大庆分院 | Plant-based compound nematicide and preparation method thereof |
| CN105707142A (en) * | 2016-02-16 | 2016-06-29 | 哈尔滨凝昇科技有限公司 | Pesticide composition for preventing and treating soybean nematode worms and preparing method thereof |
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| US20100113352A1 (en) * | 2008-11-06 | 2010-05-06 | Elliott Millstein | Retinol formulations and methods for their use |
| CN102246831A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Peony bark nematicidal extract and preparation method thereof |
| CN102246829A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Clove extract for killing nematode and preparation method thereof |
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2012
- 2012-07-02 CN CN2012102422545A patent/CN102726455A/en active Pending
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| US20100113352A1 (en) * | 2008-11-06 | 2010-05-06 | Elliott Millstein | Retinol formulations and methods for their use |
| CN102246831A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Peony bark nematicidal extract and preparation method thereof |
| CN102246829A (en) * | 2011-07-28 | 2011-11-23 | 北京农学院 | Clove extract for killing nematode and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104522065A (en) * | 2015-02-03 | 2015-04-22 | 黑龙江省科学院大庆分院 | Plant-based compound nematicide and preparation method thereof |
| CN104522065B (en) * | 2015-02-03 | 2017-05-03 | 黑龙江省科学院大庆分院 | Plant-based compound nematicide and preparation method thereof |
| CN105707142A (en) * | 2016-02-16 | 2016-06-29 | 哈尔滨凝昇科技有限公司 | Pesticide composition for preventing and treating soybean nematode worms and preparing method thereof |
| CN105707142B (en) * | 2016-02-16 | 2018-11-09 | 河南柏裕植物免疫科技有限公司 | A kind of pharmaceutical composition and preparation method thereof of prevention soybean nematode |
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Application publication date: 20121017 |