CN106109961A - The preparation method of Herba Dendrobii breaking cellular wall decoction pieces - Google Patents
The preparation method of Herba Dendrobii breaking cellular wall decoction pieces Download PDFInfo
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- CN106109961A CN106109961A CN201610646578.3A CN201610646578A CN106109961A CN 106109961 A CN106109961 A CN 106109961A CN 201610646578 A CN201610646578 A CN 201610646578A CN 106109961 A CN106109961 A CN 106109961A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
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Abstract
The present invention provides the preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces, by using medical material through picking cleaning/process of preparing Chinese medicine, it is dried, broken, the pulverizer of 100 mesh sieves is pulverized, batch (-type) sterilizes, breaking cellular wall, pelletize, it is dried and prepares Herba Dendrobii breaking cellular wall decoction pieces, the particle type of the decoction pieces of preparation is preferable, elasticity is moderate, and the granule prepared is easier to be dried, granule leaches comparatively fast, it is easily dispersed when user is taken, the decoction pieces that batch (-type) sterilization is meets the requirement of microbial limit, solve Herba Dendrobii breaking cellular wall decoction pieces in the market and leach the difficult and difficult problem up to standard of microbial limit.
Description
Technical field
The present invention relates to Chinese medicine processing technology field, particularly belong to the preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces.
Background technology
Superfine communication technique is the new technique developed rapidly in recent years.Effective ingredient in Chinese crude drug is distributed in mostly
Intracellular, conventional decoction pieces can only make part effective ingredient discharge when decocting, utilization rate of active components 10-30%；And use
Wall breaking pulverization technology, as being crushed to about 300 mesh by the prepared slices of Chinese crude drugs, cell-wall breaking ratio is up to 86.7%, improves in medical material
The dissolution of effective ingredient, is greatly enhanced its drug effect, and utilization rate of active components, more than 90%, reaches to reduce medical material usage amount and guarantor
Protecting herb resource, the quality that the most also can improve medicine increases drug effect.Herba Dendrobii, also known as Herba hedyotis costatae, Yunnan iron sheet, iron sheet
Bucket, Herba Dendrobii (Dendrobium officinale Kimura et Migo) is the orchid family Dendrobium Sw, is China's ancient Chinese prose
One of orchid recorded the earliest in offering.The kind of Herba Dendrobii, just there is kind more than 1500 in the whole world, and China there are about 76 kinds.Iron sheet stone
Dry measure used in former times is a kind the famousst and precious, that medical value is the highest, and Shennong's Herbal is classified as top grade, it is indicated that Herba Dendrobii
Have promote the production of body fluid, quench the thirst, the effect of edema of easing pain, eliminate, cure mainly the disease such as the calentura deficiency of YIN, mesh is dark, stomach is weak, hoarseness, to sound
Band is tired, hoarseness, and recovering its U.S. sound has special efficacy.Herba Dendrobii is classified as China's nine big Herba mesonae chinensis by Taoist school's ancient books and records " Taoist Scriptures "
First of.Modern study shows, the main component of Herba Dendrobii include polysaccharide, alkaloid, compounds, sesquiterpenoids, phenolic acid,
Lignanoids etc..The polysaccharide composition of Herba Dendrobii can promote humoral immunity of organism, cellular immune function and induce various kinds of cell
The factor, has the effect of enhancing human body immunity power, and compounds then has obvious antitumor action.Dendrobium candidum tissue is trained
Foster thing has the ability of stronger Scavenger of ROS, by significantly improving superoxide dismutase (SOD) level, reduces peroxidating
Lipid (LPO) acts on, and finally removes interior free yl.Herba Dendrobii also has the effects such as resisting fatigue, blood sugar lowering, eliminating phlegm and relieving cough,
At the auxiliary treatment of malignant tumor, chronic gastritis, diabetes, chronic pharyngitis, weakness due to chronic disease immunologic hypofunction, eye health etc.
Aspect is all widely used.Owing to Herba Dendrobii natural propagation rate is extremely low, adds people and excavate the most without limit, cause
This species wild resource is the poorest, has the most been listed in national secondary endangered protection plant.For promoting active ingredient in vivo
Absorption, improve the utilization rate of drug resource and extraction efficiency, the research broken to dendrobium candidum ultrafine powder is highly desirable to.
CN 201410254986.5 discloses the preparation method of a kind of dendrobium candidum ultrafine powder, comprises the steps: 1) locate in advance
Reason: take fresh Herba Dendrobii medical material, removes impurity, washes, cut into chunks；2) freezing；3) low-temperature grinding obtains 40～100
Purpose coarse powder；4) low-temperature vacuum microwave is dried, and obtains Herba Dendrobii xeraphium；5) low temperature supersonic jet mill to 300 mesh with
Under, obtain dendrobium candidum ultrafine powder；6) co-vibration mill is pulverized, and makes particle diameter reach, between 10～300 nanometers, to obtain superfine powder；7) divide
Dress and packaging, prepare dendrobium candidum ultrafine powder finished product.
CN200610122171.7 discloses the processing method of a kind of traditional Chinese herbs broken wall powder, and the step of its processing is: (1) medical material
Concoct: it includes selection, section, dried clean medical material；(2) pulverize: with above-mentioned clean medical material, with the omnipotent powder of 100 mesh sieves
Broken machine is pulverized, and wall breaking pulverization obtains the 60 above coarse powder of mesh；(3) wall breaking pulverization: by coarse powder flour obtained as above, through wall cell disruption powder powder
It is broken to more than 300 mesh, obtains superfine powder；(4) pelletize: take superfine powder obtained as above, put in flute profile mixing and blending machine, use water
Or ethanol makes soft material, pelletizing through the oscillating granulator of 30 mesh sieves, the wet granular prepared is put in heated-air circulation oven and is dried；
(5) granulate, screening: with upper strata 40 mesh, above-mentioned granulation granulate is sieved 1-3 time, makes 40 by the vibration pelletizing machine of lower floor 80 mesh sieve
Granule between mesh-80 mesh；(6) subpackage and packaging: take qualified sub-micron-powder, subpackage and outer package and obtain finished product.
According to current technique, the Herba Dendrobii breaking cellular wall decoction pieces of preparation, its granule not only fastens, and granule is less,
It is difficult to during actually used leach, is unfavorable for the Fast Stripping of active ingredient；Medical material only selection, section, special to needs
The medical material concocted is inapplicable；For entering whether the coarse powder of clean area meets microbial limit and do not provide processing method.
Summary of the invention
For the deficiencies in the prior art, the present invention provides the preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces, the party's legal system
Standby Herba Dendrobii breaking cellular wall decoction pieces particle type is preferable, degree of tightness appropriateness, is easier to be dried, and granule leaches soon, and active ingredient is molten
Going out fast, the microbial limit of products obtained therefrom meets the requirements, and can guarantee that drug safety, with effectively, uses iron sheet stone prepared by the method
The time of dry measure used in former times breaking cellular wall decoction pieces is short, and process costs is low.
For solving above-mentioned technical problem, the present invention provides following technical scheme: the preparation of a kind of Herba Dendrobii breaking cellular wall decoction pieces
Method, described medical material through picking clean/concoct, be dried, broken, the pulverizer pulverizing of 100 mesh sieves, batch (-type) sterilization, breaking cellular wall,
Pelletize, be dried prepared Herba Dendrobii breaking cellular wall decoction pieces.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces, has following steps:
(1) medical material processes: medical material Herba Dendrobii is through picking cleaning/process of preparing Chinese medicine, medicinal material drying, broken, the pulverizing of 100 mesh sieves
Machine prepares the medicinal material coarse powder of 80～120 mesh after pulverizing；
(2) batch (-type) sterilization: the medicinal material coarse powder after step (1) being processed carries out sterilizing-and hypopus grow-sterilizes again
Process, until obtaining meeting the medicinal material coarse powder directly taking decoction pieces microbial limit；
(3) breaking cellular wall: the medicinal material coarse powder after sterilization is ground into superfine powder；
(4) soft material processed: the superfine powder that breaking cellular wall prepares is placed in blender, makes soft material with the ethanol of water or variable concentrations；
(5) pelletize: soft material is made granule；
(6) Herba Dendrobii breaking cellular wall decoction pieces will be prepared after particle drying.
Described step (1) Chinese crude drug is dried and refers to that medical material Herba Dendrobii is dried to water content below 10%；Described step
(1) crush in and refer to the medical material Herba Dendrobii mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh.
What in described step (2), batch (-type) sterilized concretely comprises the following steps: (A) sterilizes: the Herba Dendrobii after step (1) being processed
Medicinal material coarse powder is placed in climatic chamber and keeps drying sterilizing a period of time under uniform temperature；(B) hypopus growth: medical material is thick
Powder temperature in climatic chamber is 20～30 DEG C, relative humidity 70～80% environment in maintain 8～24 hours, then by constant temperature
The temperature of constant humidity cabinet be adjusted to 33～40 DEG C, relative humidity be adjusted to 83～92% maintain 8～24 hours；(C) sterilization again: enter
Medicinal material coarse powder is placed in climatic chamber drying sterilizing a period of time at a certain temperature by step ground；Conventional sense medicinal material coarse powder
In microorganism situation, prepare meet the medicinal material coarse powder directly taking decoction pieces microbial limit；Described uniform temperature is 50～80
℃；Described a period of time is 0.5～8 hour；The thickness that described medicinal material coarse powder is placed in climatic chamber is 5mm～30mm.
Further, the concretely comprising the following steps of batch (-type) sterilization in described step (2): (A) sterilizes: in climatic chamber 60
～at 70 DEG C, keep drying sterilizing 0.5～4h；(B) hypopus growth: medicinal material coarse powder temperature in climatic chamber is 20～30
DEG C, relative humidity 70～80% environment in maintain 12～15 hours；Then the temperature of climatic chamber is adjusted to 33～40
DEG C, relative humidity 83～92% environment in maintain 12～15 hours；(C) sterilization again: at climatic chamber 60～70 DEG C
Keep drying sterilizing 0.5～4h.
What in described step (2), batch (-type) sterilized concretely comprises the following steps: (A) sterilizes: the Herba Dendrobii after step (1) being processed
Coarse powder is placed and is kept drying sterilizing a period of time under uniform temperature in an oven；(B) hypopus growth: will be after sterilization processing
Herba Dendrobii coarse powder take out from baking oven, pack, when temperature is 20～30 DEG C, in bag, be filled with sealing after water vapour maintain
8～24 hours；(C) sterilization again: the Herba Dendrobii coarse powder after hypopus grows is placed in an oven in uniform temperature
Lower drying sterilizing a period of time；Microorganism situation in conventional sense Herba Dendrobii coarse powder, prepares and meets that directly to take decoction pieces micro-
The Herba Dendrobii coarse powder of biological limit；Described uniform temperature is 50～80 DEG C；Described a period of time is 0.5～8 hour；Described ferrum
The thickness that skin Herba Dendrobii coarse powder is placed in climatic chamber is 5mm～30mm.
Further, in described step (2) in the concrete steps of batch (-type) sterilization: described a period of time is 0.5～4 little
Time；Described (B) hypopus grows: maintaining growth time is 12～15 hours.
Further, after in described step (2), batch (-type) has sterilized, the microorganism in conventional sense medicinal material coarse powder is not
Meet when directly taking decoction pieces microbial limit, repeat the growth of (B) hypopus and (C) sterilization process 1～4 times；Prepare and meet directly
Connect the medicinal material coarse powder taking decoction pieces microbial limit.
Further, in described step (2), the bag in batch (-type) sterilization is plastic bag or Fibre Bag etc..
Further, described step (2) is filled with water vapour to bag in batch (-type) sterilization, refers to be charged to by sack completely, bag
Son is supported in shape.
Described step (5) is pelletized method particularly includes: the granulation of Herba Dendrobii soft material 14～24 mesh sieves that will make
Machine is pelletized, and adds superfine powder while adding soft material in granulator, completes until preparing granule；Described granulator is 14
～24 mesh sieve oscillating granulator or squash type granulator.
In the concrete grammar pelletized in described step (5), the ratio of soft material and superfine powder is 3～6:0.5～1.
In described step (6), particle drying is carried out in heated-air circulation oven, design temperature 50～80 DEG C, be dried to
Moisture is less than or equal to 7%.
In described step (6), granule completes after drying, with upper strata 10 mesh, the concussion pelletizing machine granulate screening of bottom 24 mesh sieve
1～3 time, make granule between 10～24 mesh.
Breaking cellular wall in described step (3) method particularly includes: at room temperature and relative humidity in the environment of 30～70%, passes through
The Herba Dendrobii medicinal material coarse powder of batch (-type) sterilization obtains superfine powder through wall breaking pulverization, controls operating pressure 0.5～0.8MPa,
Grader rotating speed is 20～40r/s, more than wall breaking pulverization to 300 mesh；Described room temperature refers to 18～26 DEG C.
In described step (4), blender is trough type mixing machine or wet mixing pelletizer, and the ethanol of described variable concentrations is
The concentration of finger ethanol is 30%～99%.
Further, in the concrete grammar pelletized in described step (5), by step 4) the superfine powder soft material that makes puts into
Oscillating granulator forward direction screen cloth spills a small amount of superfine powder, to reduce the situation of mesh screen blocking.
The screening that the present invention uses the screen cloth clearly pelletized to carry out mesh number makes granularity moderate；Limit is used in pelletization
Adding soft material limit and add the method granulation of breaking cellular wall dry powder, the particle type of gained is preferable, and elasticity is moderate, and the granule prepared is easier to
Being dried, granule leaches comparatively fast.
The sterilization process repeatable operation of medicinal material coarse powder can strengthen sterilization effect, and cycle-index is the first shadow of sterilization process
The factor of sound, circulates the level that substantially can reach to meet the microbial limit directly taking decoction pieces 2 times.Traditional is xeothermic and damp and hot
The high temperature that sterilizing is both needed to more than 80 degrees Celsius processes, and material component exists certain destruction, especially to containing heat
The medical material of quick property composition is inapplicable.The present invention uses the method for batch (-type) sterilizing, the temperature of sterilization process at 50～80 degrees Celsius,
In the environment of keeping being dried, several without destroying to the composition of medical material Herba Dendrobii, and grow through the brood body-hypopus that goes out-go out
The process of brood body, in the environment being grown in high humidity of hypopus, after hypopus grows into brood body, kills, kills
Effect is obvious, the preparation of follow-up Herba Dendrobii breaking cellular wall decoction pieces, meets the requirement of the microbial limit directly taking decoction pieces.
Beneficial effects of the present invention:
The present invention provides the preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces, by use medical material through picking cleaning/process of preparing Chinese medicine,
Be dried, broken, pulverizer pulverizings of 100 mesh sieves, batch (-type) sterilization, breaking cellular wall, limit add soft material limit and add the method granulation of breaking cellular wall dry powder, dry
Preparing Herba Dendrobii breaking cellular wall decoction pieces, the particle type of the decoction pieces of preparation is preferable, and elasticity is moderate, and the granule prepared is easier to be dried,
Granule leaches comparatively fast, is easily dispersed when user is taken, and batch (-type) sterilization completes within one device so that decoction pieces meets micro-
The requirement of biological limit, solves Herba Dendrobii breaking cellular wall decoction pieces in the market and leaches the difficult and difficult problem up to standard of microbial limit.
By killing the brood body of antibacterial in medicinal material coarse powder at a certain temperature, then under conditions of room temperature high humidity, allow dormancy
Body grows up to bacterial propagule, then through killing the brood body of the antibacterial in medicinal material coarse powder at a certain temperature, 0～4 time the most repeatedly,
Thus the purpose that reaches to sterilize the most thoroughly；Method for disinfection, can not only ensure with the Herba Dendrobii being prepared by the method for the present invention
Breaking cellular wall decoction pieces meets the microbial limit requirement directly taking decoction pieces, and is able to ensure that the drug safety of Herba Dendrobii breaking cellular wall decoction pieces
And drug effect.The sterilising temp of the medicinal material coarse powder of preparation is 50～80 degrees Celsius, several without impact on the content of medical material Herba Dendrobii, thus
Guarantee drug effect and sterilizing；Do not have the situation of the oxidized degeneration of effective ingredient of medical material Herba Dendrobii；The time of method is short, power consumption
Low.It addition, the present invention uses climatic chamber, it is effectively guaranteed the growing environment of hypopus in medicinal material coarse powder, can have simultaneously
Effect prevents occurring the situation that foreign body, dust enter medicinal material coarse powder when batch (-type) sterilizing owing to medicinal material coarse powder moves, and effectively prevents
Only secondary pollution, it can be ensured that the drug safety of Herba Dendrobii breaking cellular wall decoction pieces and drug effect.It addition, the present invention uses is filled with water in bag
Steam, is effectively guaranteed the humidity of the growing environment of hypopus in medicinal material coarse powder, and pack simultaneously can effectively prevent due to medical material
Coarse powder occurs that in hypopus growth course foreign body, dust enter the situation of medicinal material coarse powder, effectively prevents secondary pollution, it is possible to really
Protect drug safety and the drug effect of breaking cellular wall decoction pieces.The method granulation of breaking cellular wall dry powder, the particle of gained is added by using limit to add soft material limit
Type is preferable, and elasticity is moderate, and the granule prepared is easier to be dried, and granule leaches comparatively fast.The method that the present invention provides was being pelletized
Adding Herba Dendrobii breaking cellular wall decoction pieces dry powder in journey, prepared granule elasticity is moderate, the later stage is dried, granulate strong, in use
It is easily dispersed when person takes, solves Herba Dendrobii breaking cellular wall decoction pieces in the market and leach the difficult and difficult problem up to standard of microbial limit.
The preparation method that the present invention provides is simple, easily operates, low cost, is suitable for commercial Application.
Detailed description of the invention
With specific embodiment, the present invention is further illustrated below, but the present invention is not limited by following embodiment.
Embodiment 1
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material process: by Herba Dendrobii medical material through pick cleaning/process of preparing Chinese medicine, medicinal material drying to medical material water content 10%
Hereinafter, the medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches further
To about 100 mesh；
2) batch (-type) sterilization: Herba Dendrobii coarse powder is put in climatic chamber, places thickness and is about 10mm, and arranging temperature is
65 DEG C (actual temperature is 60～70 DEG C), keeps drying sterilizing 8h；Medicinal material coarse powder after process is 25 DEG C, relative humidity in temperature
75% maintains 12 hours；Then the temperature of climatic chamber is adjusted to 35 DEG C, relative humidity 88% maintain 24 hours；Change sets
Putting temperature is 75 DEG C (actual temperature is 70～80 DEG C), keeps drying sterilizing 4h；Microorganism feelings in conventional sense medicinal material coarse powder
Condition, must meet the Herba Dendrobii coarse powder directly taking decoction pieces microbial limit requirement；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 30%, warp
Wall breaking pulverization, controls operating pressure and more than 20r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.6MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 50～60% ethanol, makes
Soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 16 mesh, and during granulation, limit adds step 4) the wall cell disruption powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, soft material is 3:1 with the ratio of superfine powder；
6) it is placed in 70 DEG C～80 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 2 times, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Embodiment 2
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material process: by Herba Dendrobii medical material through pick clean/concoct, be dried to medical material water content 10% with
Under, the medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches further
About 100 mesh；
2) batch (-type) sterilization: Herba Dendrobii coarse powder is put in climatic chamber, places thickness and is about 5mm, and arranging temperature is 70
DEG C (actual temperature is 65～75 DEG C), keeps drying sterilizing 4h；Medicinal material coarse powder after process is 20 DEG C, relative humidity 70% in temperature
Maintain 15 hours；Then the temperature of climatic chamber is adjusted to 33 DEG C, relative humidity 83% maintain 8 hours；Change arranges temperature
It is 65 DEG C (actual temperature is 60～70 DEG C), keeps drying sterilizing 6h；Repeat hypopus grow-again to go out bacterial propagule step 1 time,
Microorganism situation in conventional sense medicinal material coarse powder；The Herba Dendrobii coarse powder directly taking decoction pieces microbial limit requirement must be met；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 50%, warp
Wall breaking pulverization, controls operating pressure and more than 40r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.5MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 30～40% ethanol, makes soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 20 mesh, and during granulation, limit adds step 4) the superfine powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, the ratio of soft material and superfine powder is 3:0.5；
6) it is placed in 60 DEG C～70 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 1 time, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Embodiment 3
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material processes: by Herba Dendrobii medical material through picking the water content cleaned/concoct, be dried to medical material below 10%, will
The medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches about 100 mesh further；
2) batch (-type) sterilization: Herba Dendrobii coarse powder is put in climatic chamber, places thickness and is about 30mm, and arranging temperature is
75 DEG C (actual temperature is 70～80 DEG C), keeps drying sterilizing 4h；Medicinal material coarse powder after process is 25 DEG C, relative humidity in temperature
75% maintains 24 hours；Then the temperature of climatic chamber is adjusted to 40 DEG C, relative humidity 80% maintain 12 hours；By medical material
Coarse powder is in climatic chamber, and placement thickness is 10mm, and arranging temperature is 80 DEG C (actual temperature is 75～85 DEG C), keeps being dried
Sterilization 0.5h；Repeat step 4 time, the microorganism situation in conventional sense medicinal material coarse powder；Must meet and directly take decoction pieces microorganism
The Herba Dendrobii coarse powder of bound requirements；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 70%, warp
Wall breaking pulverization, controls operating pressure and more than 30r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.8MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 60～70% ethanol, makes
Soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 20 mesh, and during granulation, limit adds step 4) the superfine powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, the ratio of soft material and superfine powder is 6:1；
6) it is placed in 50 DEG C～60 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 3 times, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Embodiment 4
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material process: by Herba Dendrobii medical material through pick clean/concoct, be dried to medical material water content 10% with
Under, the medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches further
About 100 mesh；
2) batch (-type) sterilization: Herba Dendrobii coarse powder is put in climatic chamber, often dish placement thickness is about 25mm, arranges temperature
Degree is 55 DEG C (actual temperature is 50～60 DEG C), keeps drying sterilizing 0.5h；Be adjusted to 30 DEG C, relative humidity 80% ties up
Hold 8 hours, be adjusted to 37 DEG C, relative humidity 92% maintain 15 hours；Medicinal material coarse powder is put in climatic chamber, often
Thickness placed by dish is 5mm, and arranging temperature is 55 DEG C (actual temperature is 50～60 DEG C), keeps drying sterilizing 8h；Conventional sense medicine
Microorganism situation in material coarse powder, is repeated 2 times again, the microorganism situation in conventional sense medicinal material coarse powder；Directly clothes must be met
The Herba Dendrobii coarse powder required with decoction pieces microbial limit；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 40%, warp
Wall breaking pulverization, controls operating pressure and more than 30r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.7MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 80～99% ethanol, makes soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 20 mesh, and during granulation, limit adds step 4) the wall cell disruption powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, soft material is 6:0.5 with the ratio of superfine powder；
6) it is placed in 50 DEG C～60 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 3 times, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Embodiment 5
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material process: by Herba Dendrobii medical material through pick cleaning/process of preparing Chinese medicine, medicinal material drying to medical material water content 10%
Hereinafter, the medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches further
To about 100 mesh；
2) batch (-type) sterilization: go out bacterial propagule: Herba Dendrobii coarse powder is put in baking oven, places thickness and is about 5mm, arrange
Temperature is 70 DEG C (actual temperature is 65～75 DEG C), keeps drying sterilizing 4h；Hypopus grows: the medicinal material coarse powder after process is from baking
Taking out in case, pack, when temperature is 20 DEG C, after being filled with water vapour in bag, sealing maintains 15 hours；Go out bacterial reproduction again
Body: medicinal material coarse powder be put in baking oven, placement thickness is 20mm, and arranging temperature is 65 DEG C (actual temperature is 60～70 DEG C), protects
Hold drying sterilizing 6h；Repeat hypopus grow-again to go out bacterial propagule step 1 time, the micro-life in conventional sense medicinal material coarse powder
Principle condition；The Herba Dendrobii coarse powder directly taking decoction pieces microbial limit requirement must be met；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 30%, warp
Wall breaking pulverization, controls operating pressure and more than 20r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.6MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 50～60% ethanol, makes soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 16 mesh, and during granulation, limit adds step 4) the superfine powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, the ratio of soft material and superfine powder is 5:1；
6) it is placed in 70 DEG C～80 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 2 times, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Embodiment 6
Herba Dendrobii breaking cellular wall decoction pieces
1) medical material processes: by Herba Dendrobii medical material through picking the water content cleaned/concoct, be dried to medical material below 10%, will
The medical material mechanical crusher of the screen cloth of 10 mesh is broken into the little granule about 10 mesh；Coarse pulverization medical material reaches about 100 mesh further；
2) batch (-type) sterilization: go out bacterial propagule: Herba Dendrobii coarse powder is placed in an oven, places thickness and is about 10mm,
Arranging temperature is 65 DEG C (actual temperature is 60～70 DEG C), keeps drying sterilizing 8h；Hypopus grows: the medicinal material coarse powder after process
Taking out from baking oven, pack, when temperature is 25 DEG C, after being filled with water vapour in bag, sealing maintains 12 hours；Go out bacterial reproduction
Body: medicinal material coarse powder be put in baking oven, placement thickness is 30mm, and arranging temperature is 75 DEG C (actual temperature is 70～80 DEG C), protects
Hold drying sterilizing 4h；Microorganism situation in conventional sense medicinal material coarse powder, must meet and directly take decoction pieces microbial limit requirement
Herba Dendrobii coarse powder；
3) breaking cellular wall: by particle diameter the medicinal material coarse powder of 100～120 mesh at room temperature and relative humidity in the environment of 50%, warp
Wall breaking pulverization, controls operating pressure and more than 40r/s, wall breaking pulverization to 300 mesh, obtains ultra-fine at 0.5MPa, grader rotating speed
Powder body (90% powder size is less than 45 microns)；
4) soft material processed: be placed in trough type mixing machine by prepared superfine powder, adds appropriate 30～40% ethanol, makes soft material；
5) pelletize: by step 4) the superfine powder soft material that makes put into oscillating granulator forward direction screen cloth spills the most ultra-fine
Powder body, pelletizes with the screen cloth of 20 mesh, and during granulation, limit adds step 4) the superfine powder soft material that makes time limit add step 3) prepare
Dendrobium candidum ultrafine powder body, the ratio of soft material and superfine powder is 4:0.8；
6) it is placed in 60 DEG C～70 DEG C of baking ovens is dried taking qualified breaking cellular wall decoction pieces granule, dried granule upper strata 10
Mesh, the concussion pelletizing machine granulate of bottom 24 mesh sieve sieves 1 time, and subpackage i.e. obtains Herba Dendrobii breaking cellular wall decoction pieces.
Leach time detecting test
Test method: take about 2g prepare dry granule be placed in the transparent cup of 300 milliliters, add 90 degrees Celsius Celsius to 95
100 milliliters of the water (being no longer heat up during shaking) of degree, shakes 1 minute, 2 minutes, 3 points with the clockwise direction of 1 circle per second
Clock, 5 minutes, see granule in cup after 10 minutes leach situation, separately take the American-European gram LS-609 laser grain of liquid 0.5 milliliter in cup
Degree instrument detection shading rate, result of the test see table 1:
Knowable to table 1 data, the superfine powder method of granulating that the present invention provides is more preferable than the effect of conventional method of granulating, molten
Go out diffusion rate than conventional method faster.
Microbial limit detection test
By " Chinese Pharmacopoeia " 2015 editions the 4th the 140-151 page
1105 non-sterile product microbial limit detections: microorganism count method
1106 non-sterile product microbial limit detections: Control bacteria examination method
1107 non-sterile medicine limitation standard in microbes
According to the method for pharmacopeia, the sample of embodiment 1～6 is carried out microbial limit detection to test, concrete outcome such as table 2:
The detection test of table 2 microbial limit
Knowable to table 2 data, batch (-type) sterilizing methods is more preferable than conventional dry heat sterilization effect, more can guarantee that hygiology matter
Amount.
Active constituent content measuring is tested
By " Chinese Pharmacopoeia " 2015 editions first
Page 282 is about Herba Dendrobii detection method of content
Medicinal material coarse powder after sterilizing the batch (-type) of embodiment 1～6 according to the regulation of pharmacopeia carries out assay, specifically ties
Fruit is such as table 3:
The active constituent content measuring test of the medicinal material coarse powder after the sterilization of table 3 batch (-type)
As known from Table 3, the batch (-type) sterilizing methods that the present invention uses is several without impact, energy on the medical material content of embodiment 1～6
The effective active constituent content keeping medicinal material coarse powder.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, that is made any repaiies
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. the preparation method of a Herba Dendrobii breaking cellular wall decoction pieces, it is characterised in that there are following steps:
(1) medical material processes: Herba Dendrobii is through picking cleanings/processs of preparing Chinese medicine, medicinal material drying, crushing, after the pulverizing of the pulverizer of 100 mesh sieves
Prepare the Herba Dendrobii coarse powder of 80～120 mesh；
(2) batch (-type) sterilization: the Herba Dendrobii coarse powder after step (1) being processed carries out sterilizing-and hypopus grow-sterilizes again
Process, until obtaining meeting the Herba Dendrobii coarse powder directly taking decoction pieces microbial limit；
(3) breaking cellular wall: the Herba Dendrobii coarse powder after sterilization is ground into dendrobium candidum ultrafine powder body；
(4) soft material processed: the dendrobium candidum ultrafine powder body that breaking cellular wall prepares is placed in blender, by water or the ethanol system of variable concentrations
Become soft material；
(5) pelletize: soft material is made Herba Dendrobii granule；
(6) Herba Dendrobii breaking cellular wall decoction pieces will be prepared after particle drying.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claim 1, it is characterised in that described step
(2) what in, batch (-type) sterilized concretely comprises the following steps: (A) sterilizes: the Herba Dendrobii medicinal material coarse powder after step (1) being processed is placed on perseverance
Constant temperature and humidity case keeps drying sterilizing a period of time under uniform temperature；(B) hypopus growth: by medicinal material coarse powder at climatic chamber
Middle temperature is 20～30 DEG C, relative humidity 70～80% environment in maintain 8～24 hours, then by the temperature of climatic chamber
Be adjusted to 33～40 DEG C, relative humidity be adjusted to 83～92% maintain 8～24 hours；(C) sterilization again: further by medical material
Coarse powder is placed in climatic chamber drying sterilizing a period of time at a certain temperature；Microorganism in conventional sense medicinal material coarse powder
Situation, prepares and meets the medicinal material coarse powder directly taking decoction pieces microbial limit；Described uniform temperature is 50～80 DEG C；Described one section
Time is 0.5～8 hour；The thickness that described medicinal material coarse powder is placed in climatic chamber is 5mm～30mm.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claim 1, it is characterised in that described step
(2) what in, batch (-type) sterilized concretely comprises the following steps: (A) sterilizes: the Herba Dendrobii coarse powder after step (1) being processed is placed in an oven
Keep drying sterilizing a period of time under uniform temperature；(B) hypopus growth: by the Herba Dendrobii coarse powder after sterilization processing from
Taking out in baking oven, pack, when temperature is 20～30 DEG C, after being filled with water vapour in bag, sealing maintains 8～24 hours；(C) again
Secondary sterilization: when the Herba Dendrobii coarse powder after hypopus grows is placed drying sterilizing one section the most at a certain temperature
Between；Microorganism situation in conventional sense Herba Dendrobii coarse powder, prepares and meets the iron sheet stone directly taking decoction pieces microbial limit
Dry measure used in former times coarse powder；Described uniform temperature is 50～80 DEG C；Described a period of time is 0.5～8 hour；Described Herba Dendrobii coarse powder is placed on
Thickness in climatic chamber is 5mm～30mm.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claims 2 to 3, it is characterised in that described step
Suddenly, after in (2), batch (-type) has sterilized, the microorganism in conventional sense medicinal material coarse powder does not meets directly takes decoction pieces microorganism limit
When spending, repetition (B) hypopus growth and (C) sterilization process 1～4 times；Prepare and meet the medicine directly taking decoction pieces microbial limit
Material coarse powder.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claims 1 to 3, it is characterised in that described step
Suddenly (5) pelletize method particularly includes: the soft material the made granulator of 14～24 mesh sieves is pelletized, in granulator
Adding dendrobium candidum ultrafine powder body while adding soft material, completing until preparing granule；Described granulator is waving of 14～24 mesh sieves
Formula granulator or squash type granulator.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claim 5, it is characterised in that described step
(5), in the concrete grammar pelletized in, the ratio of soft material and dendrobium candidum ultrafine powder body is 3～6:0.5～1.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claims 1 to 3, it is characterised in that described step
Suddenly (1) Chinese crude drug is dried and refers to that Herba Dendrobii is dried to water content below 10%；Described step (1) crushes and refers to iron sheet
The Herba Dendrobii mechanical crusher of the screen cloth of 10 mesh is broken into the little granule of the Herba Dendrobii about 10 mesh.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claims 1 to 3, it is characterised in that described step
Suddenly in (6), particle drying is carried out in heated-air circulation oven, design temperature 50～80 DEG C, is dried to moisture and is less than or equal to
7%；In described step (6), granule completes after drying, with upper strata 10 mesh, the concussion pelletizing machine granulate screening 1～3 of bottom 24 mesh sieve
Secondary, make granule between 10～24 mesh.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claims 1 to 3, it is characterised in that described broken
Breaking cellular wall in step (3) method particularly includes: at room temperature and relative humidity in the environment of 30～70%, through step (2) intermittently
The Herba Dendrobii coarse powder of formula sterilization obtains dendrobium candidum ultrafine powder body through wall breaking pulverization, controls operating pressure 0.5～0.8MPa,
Grader rotating speed is 20～40r/s, more than wall breaking pulverization to 300 mesh；Described room temperature refers to 18～26 DEG C.
The preparation method of a kind of Herba Dendrobii breaking cellular wall decoction pieces the most according to claim 9, it is characterised in that described step
(4) in, blender is trough type mixing machine or wet mixing pelletizer, and the ethanol of described variable concentrations refers to that the concentration of ethanol is
30%～99%.
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| CN108310228A (en) * | 2018-04-04 | 2018-07-24 | 安徽颐生堂中药饮片有限公司 | A kind of stem of noble dendrobium medicine materical crude slice and its concocting method |
| CN112755129A (en) * | 2020-12-31 | 2021-05-07 | 江苏传承中药有限公司 | Processing method and processing equipment based on traditional Chinese medicine decoction pieces |
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