A kind of pharmaceutical composition and preparation method thereof of prevention soybean nematode
Technical field
The invention belongs to the pharmaceutical compositions and its preparation side of control of nematode field more particularly to a kind of prevention soybean nematode
Method.
Background technology
Soybean nematodiasis is also known as root nematode of soybean, sallow nematodiasis.It is commonly called as " flue seedling sprout ".Seedling stage catches an illness diseased plant cotyledon
Turn yellow with true leaf, fertility is stagnated and withered.Killed plant is short and small, bud fasciation, internode cripetura, florescence delay, cannot bear pods or
It bears pods few, yellowing leaf.Grave illness strain flower and green bean are withered, whole strain leaf is withered and yellow from bottom to top seemingly burns shape.Root system is caught an illness parasitic
The micro- cyst such as flour grain of white spar is exposed in the bulge of main root side or rupture, root is killed seldom or non-nodulating, since cyst supports
Broken root skin, root liquid extravasation, causes secondary soil root disease to aggravate or cause root-rot.
For the illness mainly caused by soybean cyst nematode Heterodera glycines, which is a kind of sedentariae endoparasitism nematode, with 2 ages children
Worm is movable in soil, point intrusion of looking for one's roots.The nematosis pulse family, Scrophulariaceae more than 170 plant plant, though the intrusion having, not in root
Interior development.Cyst nematode with ovum, embryo's ovum and a small amount of larva in cyst in soil it is overwintering, some is adhered to seed or farm implements
It is upper overwintering, become next year primary source of infection, cyst cutin thickness, can survive l0 or more in the soil.
Currently, the control method for soybean nematodiasis mainly has disease-resistant variety selection and breeding and medical treatment.Disease-resistant variety
The process of selection and breeding is longer and R&D costs are higher.Medical treatment generally use Isofenphos methyl water-soluble granular formulation etc. is easy production
The problems such as raw medicament residue and undesirable control effect.
Invention content
In view of the problems of prior art, the present invention provides a kind of pharmaceutical composition and its system of prevention soybean nematode
Preparation Method has many advantages, such as that good control effect, drug residue free, quick, preparation method is simple, at low cost.
The technical solution that the present invention solves above-mentioned technical problem is as follows:
A kind of pharmaceutical composition of prevention soybean nematode, includes the raw material of following parts by weight:10-15 parts of frutus cnidii, cassia bark
20-25 parts, 30-40 parts of nano-sized carbon, 20-25 parts of yellow ginger, 5-8 parts of bamboo Siberian cocklebur, 80-100 parts of maltose.
The beneficial effects of the invention are as follows:The present invention is a kind of using plant source raw material as the pharmaceutical composition of primary raw material, no
It only can all reach good control of nematode effect, and have the advantages that non agricultural chemical residuum.
Frutus cnidii, alias cicely, is the dry mature fruit of samphire cnidium monnieri.Summer, two season of autumn fruit maturation
Shi Caishou removes impurity, dries.Mainly contain firpene, isovaleric acid borneol acetate, osthole, Columbianetin, fingered citron
The ingredients such as mandarin orange lactone, Osthole, isopimpinellin.Have the function of attacking poison, desinsection.
Cassia bark, cassia bark are also known as Chinese cassia tree, official osmanthus or fragrant osmanthus, are canella cinnamomum japonicum, burmannii, cinnamomum chingii Metcalf, Chinese cassia tree or river
The common name of the barks such as osmanthus.Usually conventional Chinese medicine, and be food flavor or condiment for cooking.Inventor unexpectedly sends out under study for action
Existing, cassia bark is used cooperatively the effect that can improve control of nematode with other raw materials.
Nano-sized carbon refers to the dispersed phase scale at least one-dimensional carbon material less than 100nm.Inventor is unexpected under study for action
It was found that when nano-sized carbon is used cooperatively with other raw materials, it can not only make further increasing for the effect of control of nematode, but also soybean
Growth it is more healthy and strong, the output increased of soybean.
Yellow ginger, also known as fiery rattan root, is belonged to Dioscoreaceae dioscorea dioscorea zingiberensis wright, is used as medicine with root-like stock, usually in spring and autumn
Excavation, is dried after removing soil.The starch of the main diosgenin for containing 1.1 ﹪ -16.15 ﹪, 45 ﹪ or so, the fiber of 40 ﹪
The chemical compositions such as element and some water-soluble glycoside, alkaloids, flavonoid glycoside, cardiac glycosides, alkaloid, tannin, pigments.
Bamboo Siberian cocklebur, smell are bitter, cold, slightly poisonous peppery.
Inventor has found under study for action, and after above-mentioned various raw materials are mixed with said ratio, being applied to soybean has well
The effect of control of nematode.
Based on the above technical solution, the present invention can also be improved as follows.
Further, include the raw material of following parts by weight:12 parts of frutus cnidii, 22 parts of cassia bark, 40 parts of nano-sized carbon, 22 parts of yellow ginger,
6 parts of bamboo Siberian cocklebur, 90 parts of maltose.
Advantageous effect using the above scheme is:Further increase the effect of control of nematode.
Further, the grain size of the nano-sized carbon is 50-70 nanometers.
Advantageous effect using the above scheme is:When nano-sized carbon in the particle size range is used cooperatively with other raw materials,
Further increasing for the effect of control of nematode can not only be made, but also the growth of soybean is more healthy and strong, the output increased of soybean.
The present invention also provides a kind of preparation methods of the pharmaceutical composition of prevention soybean nematode, include the following steps:
1) frutus cnidii, cassia bark, nano-sized carbon, yellow ginger, bamboo Siberian cocklebur are ground respectively, obtains respective powder;
2) powder after step 1) grinding is weighed according to the following ratio:10-15 parts of frutus cnidii, 20-25 parts of cassia bark, nano-sized carbon
5-8 parts of 30-40 parts, 20-25 parts of yellow ginger and bamboo Siberian cocklebur;It is uniformly mixed after weighing, obtains mixture;
3) 80-100 parts by weight maltose is added in the mixture obtained to step 2) to be uniformly mixed, obtains prevention soybean line
The pharmaceutical composition of worm.
Advantageous effect using the above scheme is:Preparation method of the present invention is simple, the pharmaceutical composition being prepared into
Have many advantages, such as good control effect, drug residue free, it is quick, prepare it is simple, at low cost.
Further, in step 1), the grain size for the powder that frutus cnidii, cassia bark, yellow ginger, bamboo Siberian cocklebur are ground into is 40-500
The grain size of mesh, nano-sized carbon is 50-70 nanometers.
Further, in step 2), 12 parts by weight of frutus cnidii, 22 parts by weight of cassia bark, 40 parts by weight of nano-sized carbon, 22 weight of yellow ginger
Part and 6 parts by weight of bamboo Siberian cocklebur.
Further, in step 3), the maltose of addition is 90 parts by weight.
Advantageous effect using above-mentioned further scheme is:Being advantageously selected for of above-mentioned parameter further increases control of nematode
Effect.
Specific implementation mode
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
Each raw material used in embodiment unless otherwise noted can be by acquisition purchased in market.
Embodiment 1
1) frutus cnidii, cassia bark, nano-sized carbon, yellow ginger, bamboo Siberian cocklebur are ground respectively, obtains respective powder;Except nano-sized carbon
Grain size is 60 nanometers outer, and the powder of other raw materials is 200 mesh;
2) powder after step 1) grinding is weighed according to the following ratio:Frutus cnidii 12kg, cassia bark 22kg, nano-sized carbon 40kg,
Yellow ginger 22kg and bamboo Siberian cocklebur 6kg;It is uniformly mixed after weighing, obtains mixture;
3) 90kg maltose is added in the mixture obtained to step 2) to be uniformly mixed, obtains the drug of prevention soybean nematode
Composition.
Embodiment 2
1) frutus cnidii, cassia bark, nano-sized carbon, yellow ginger, bamboo Siberian cocklebur are ground respectively, obtains respective powder;Except nano-sized carbon
Grain size is 50 nanometers outer, and the powder of other raw materials is 40 mesh;
2) powder after step 1) grinding is weighed according to the following ratio:Frutus cnidii 10kg, cassia bark 20kg, nano-sized carbon 30kg,
Yellow ginger 20kg and bamboo Siberian cocklebur 5kg;It is uniformly mixed after weighing, obtains mixture;
3) 80kg maltose is added in the mixture obtained to step 2) to be uniformly mixed, obtains the drug of prevention soybean nematode
Composition.
Embodiment 3
1) frutus cnidii, cassia bark, nano-sized carbon, yellow ginger, bamboo Siberian cocklebur are ground respectively, obtains respective powder;Except nano-sized carbon
Grain size is 70 nanometers outer, and the powder of other raw materials is 500 mesh;
2) powder after step 1) grinding is weighed according to the following ratio:Frutus cnidii 15kg, cassia bark 25kg, nano-sized carbon 35kg,
Yellow ginger 25kg and bamboo Siberian cocklebur 8kg;It is uniformly mixed after weighing, obtains mixture;
3) 100kg maltose is added in the mixture obtained to step 2) to be uniformly mixed, obtains the medicine of prevention soybean nematode
Compositions.
Embodiment 4
Frutus cnidii is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 5
Cassia bark is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 6
Nano-sized carbon is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 7
Yellow ginger is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 8
Bamboo Siberian cocklebur is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 9
Maltose is not added, remaining formula and preparation method are the same as embodiment 1.
Embodiment 10
The dosage of frutus cnidii is adjusted to 2kg, remaining formula and preparation method are the same as embodiment 1.
Embodiment 11
The dosage of frutus cnidii is adjusted to 5kg, remaining formula and preparation method are the same as embodiment 1.
Embodiment 12
The dosage of frutus cnidii is adjusted to 20kg, remaining formula and preparation method are the same as embodiment 1.
Embodiment 13
The dosage of frutus cnidii is adjusted to 25kg, remaining formula and preparation method are the same as embodiment 1.
Embodiment 14
Frutus cnidii is replaced with 12kg cordate houttuynias, remaining formula and preparation method are the same as embodiment 1.
Embodiment 15
The mixture of the frutus cnidii tuber of stemona, Radix Glycyrrhizae and Chinese wistaria is replaced, in the mixture, tuber of stemona 4.8kg, Radix Glycyrrhizae is
4.8kg, Chinese wistaria 2.4kg, remaining formula and preparation method are the same as embodiment 1.
Test effect example
One, test method
Never the farmland for the pharmaceutical composition for trying prevention soybean nematode of the present invention is marked off into 16 homalographics
Region, the area in each region is 2 mu, wherein 1 region is check plot, in addition 15 regions are test block, and number is real
Test area 1 to test block 15.Test block 1 to test block 15 is corresponded to the medicine group applied embodiment 1 and prepared to embodiment 15 respectively
Object, i.e., the pharmaceutical composition that prepared by the application of test block 1 embodiment 1 are closed, pharmaceutical composition prepared by embodiment 2 is applied in test block 2,
Pharmaceutical composition prepared by the application embodiment 3 of test block 3, pharmaceutical composition ... ... prepared by the application embodiment 4 of test block 4 are real
Test pharmaceutical composition prepared by the application embodiment 15 of area 15.100 soybean are planted in each test block and check plot, and plant height is
6-8 inches, every soybean of test block applies 100g pharmaceutical compositions, and the soybean of check plot applies 100g water.Pharmaceutical composition
Or water is uniformly sprinkled upon at the soil of the root of soybean, pharmaceutical composition or water application position are at the 5cm of ground.Simultaneously respectively
The soil containing soybean cyst nematode Heterodera glycines is launched at the soybean root 7cm of experimental group and control group, every soybean launches 20g and contains
There is the soil of soybean cyst nematode Heterodera glycines.Other condition of culture experimental groups and control group are consistent.Observe and record the illness feelings of soybean
Condition and harvested soybean yields.
The above-mentioned soil collection containing soybean cyst nematode Heterodera glycines is used from the Soybean-planting for suffering from soybean cyst nematode
Before, by the soil mixing containing soybean cyst nematode Heterodera glycines, and it is divided into several pieces.
Two, test result
It is calculated according to above-mentioned experimental record situation and efficiency and carries out statistical analysis in catching an illness for different time, the results are shown in Table
1。
Efficiency of catching an illness refers to the ratio of the number and the number of total soybean for the soybean for having soybean cyst nematode Heterodera glycines symptom.
Soybean cyst nematode Heterodera glycines symptom refer specifically to seedling stage catch an illness diseased plant cotyledon and true leaf turn yellow, fertility stagnate it is withered.It is killed to plant
Short and small, the bud fasciation of strain, internode cripetura, florescence delay cannot bear pods or bear pods and is few, yellowing leaf.Grave illness strain flower and green bean
Withered, whole strain leaf is withered and yellow like baked wheaten cake shape from bottom to top.It is above-mentioned to there is any one to meet, it can be determined that for soybean cyst nematode Heterodera glycines
Symptom.
Table 1
The efficiency of catching an illness that can be seen that pharmaceutical composition prepared by 1-3 of the embodiment of the present invention from the result in table 1 is obviously low
In the efficiency of catching an illness of other embodiment, meanwhile, the mu of the test block at the place of pharmaceutical composition prepared by 1-3 of the embodiment of the present invention
Yield is apparently higher than other regions.Illustrate that pharmaceutical composition prepared by 1-3 of the embodiment of the present invention has by the test data of table 1
Good soybean nematode control effect, and the yield of soybean can be significantly improved.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.