CN101209059A - Novel biological preparation for killing root-knot nematode and application method thereof - Google Patents
Novel biological preparation for killing root-knot nematode and application method thereof Download PDFInfo
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- CN101209059A CN101209059A CNA2006101284650A CN200610128465A CN101209059A CN 101209059 A CN101209059 A CN 101209059A CN A2006101284650 A CNA2006101284650 A CN A2006101284650A CN 200610128465 A CN200610128465 A CN 200610128465A CN 101209059 A CN101209059 A CN 101209059A
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Abstract
A novel biological preparation for killing a root knot nematode comprises an effective component of a stemona power, which can comprise the effective component of 450 to 550 portions of the stemona power and the secondary effective components of 137 to 167 portions of soybean pod shell powder, 90 to 110 portions of pepper powder and 90 to 110 portions of tobacco leaf powder according to the weight portion; the invention can also comprise the effective component of 450 to 550 portions of the stemona power and secondary effective components of 137 to 167 portions of soybean pod shell powder, 90 to 110 portions of pepper powder and 90 to 110 portions of tobacco leaf powder, 9 to 11 portions of promoting agent MF powder, 10 to 14 portions of NNO powder, 9 to 11 portions of poly 4000 powder, 18 to 22 portions of nekal BX powder and 78 to 94 portions of carrier white carbon black power according to a weight portion. The powder relates to a wettable micro powder thinner than 500 meshes and is diluted by 200 to 1000 times to pour roots in fields or directly broadcast in fields.
Description
Technical field
The invention belongs to pesticide field, specifically is exactly plant pesticide.
Background technology
Root-knot nematode distributes wide, and the host is many, and harm is serious, falls ill to become the agriculture class phytopathy to be solved of killing that produces.The control of root-knot nematode at present is based on chemical control, but control efficiency is poor, contaminated environment, and nematode easily develops immunity to drugs.Natural plants can be regarded as the treasure-house of chemicals and bioactivator, be " chemical plant " of nature, from plant, screen, separate and both effectively suppressed pest, again to higher mammal safety, the target harmless to beneficial organism, that the botanical biological pesticide of environmentally safe has become people to be pursued.China's natural plant resource is abundant, has reported that the line plant has 226 genus of 102 sections approximately extremely, but owing to be subjected to the restriction of multiple condition, can compete with synthetic pesticide, and performance is more less for number than the natural products of U.S. with it.Having bioactive natural plants to mix by multiple, improve stability, optimize its performance, is a kind of approach of exploitation natural products biologic product.
The tuber of stemona is a kind of traditional medicinal plant of China, tuber of stemona aboundresources, and breeding is simple, contains the stemonine active substance, has the killing root-knot nematode activity, does not appear in the newspapers as yet but be developed the product that is used for the root-knot nematode control.Therefore, according to test to tuber of stemona physicochemical property, and auxilliary other active component, develop novel biological preparation for killing root-knot nematode and have broad prospects.
Summary of the invention
The objective of the invention is to utilize the natural goods of biologically active to develop a kind of novel biological preparation for killing root-knot nematode, be used for the root-knot nematode disease.
The present invention is achieved in that novel biological preparation for killing root-knot nematode, comprises active ingredient tuber of stemona powder.
Above-mentioned novel biological preparation for killing root-knot nematode is characterized in that: comprise active ingredient tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf are pressed powder 90-110.
Above-mentioned novel biological preparation for killing root-knot nematode, it is characterized in that: the active ingredient that comprises tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf press powder 90-110, activating agent MF9-11, NNO10-14, poly-second 4000 9-11, draws back powder 18-22.
Above-mentioned novel biological preparation for killing root-knot nematode, it is characterized in that: the active ingredient that comprises tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf are pressed powder 90-110, activating agent MF powder 9-11, NNO powder 10-14, poly-second 4000 powder 9-11, draw back powder powder 18-22, the white carbon black powder 78-94 of carrier.
Above-mentioned novel biological preparation for killing root-knot nematode, it is characterized in that: the active ingredient that comprises tuber of stemona powder 480-520 by weight, and inferior active ingredient soybean pod shell powder 147-157, zanthoxylum powder 95-105, tobacco leaf are pressed powder 95-105, activating agent MF powder 9.5-10.5, NNO powder 11-13, poly-second 4000 powder 9.5-10.5, draw back powder powder 19-21, the white carbon black powder 83-89 of carrier.
Above-mentioned novel biological preparation for killing root-knot nematode, it is characterized in that: the active ingredient that comprises tuber of stemona powder 506 by weight, and inferior active ingredient soybean pod shell powder 152, zanthoxylum powder 101, tobacco leaf are pressed powder 101, activating agent MF powder 10, NNO powder 12, poly-second 4000 powder 10, draw back powder powder 20, the white carbon black powder 86 of carrier.
Above-mentioned novel biological preparation for killing root-knot nematode is characterized in that: described powder is the micro mist thinner than 500 orders.
Above-mentioned novel biological preparation for killing root-knot nematode is characterized in that: said preparation is the wettable micro mist.
The using method of above-mentioned novel biological preparation for killing root-knot nematode is characterized in that: dilute 200-1000 times of field and irritate root.
The using method of above-mentioned novel biological preparation for killing root-knot nematode is characterized in that: directly spread fertilizer over the fields in the field.
Utilization of the present invention has the natural insecticidal activity plant tuber of stemona and is primary raw material, is aided with raw materials such as soybean pod shell, Chinese prickly ash, tobacco leaf and by modern superfine powder technology raw material is pulverized formulated a kind of novel killing root-knot nematode wetting powder.Have the following advantages:
(1) abundant raw materials, preparing process is simple, and is easy and simple to handle, and cost was easy to extensively promote the use of the end of than.
(2) nematode should not develop immunity to drugs, and is good with the compatibility of environment, and noresidue is pacified people and animals.
(3) this product good water solubility, high-efficiency low-toxicity, worm, ovum is double controls.
(4) this product is a raw material with the natural goods, and the promotion seed germination is arranged, and improves the effect of plant seedling growth.
Embodiment
Table 1 is the embodiment of composition of the present invention and content, unit: gram
Powder described in the table 1 is the micro mist thinner than 500 orders, also can be the micro mist of making after mixing.
Novel biological preparation for killing root-knot nematode described in the table 1 is the wettable micro mist.
Root is irritated in 200-1000 times of field of novel biological preparation for killing root-knot nematode described in the table 1 dilution.
Novel biological preparation for killing root-knot nematode field described in the table 1 is directly spread fertilizer over the fields.
It below is part laboratory report of the present invention.
Eelworm-killing activity indoor bioassay laboratory report
1. purpose and meaning
By indoor bioassay, the research soup is to the toxic effect of root-knot nematode, for the research of killing root-knot nematode preparation is provided fundamental basis.
2. method
2.1 root-knot nematode
The tomato root of picking artificial infection Meloidogyne incognita is smashed with tissue mashing machine, crosses 200 orders, 325 orders, 500 order nematode standard screens successively, with the worm's ovum on aseptic water washing 500 mesh sieves, is mixed with ovum suspension (100 ovum/ml).Other puts the part ovum hatches in sterile water, gets larva alive (2 instar larvaes are called for short J2) the confession test that 2d hatches.
2.2 the configuration of soup
By modern superfine powder technology raw material are pulverized, with the tuber of stemona, soybean pod shell, Chinese prickly ash, tobacco leaf in 10: 3: 2: 2 ratio is mixed, add 2%MF, 2.5%NNO%, 2% poly-second 4000 and 4% respectively and draw back that powder is made activating agent and 17% white carbon black is made carrier, be configured to wetting powder.200,400,800 times of soup dilutions is stand-by.
2.3 toxic effect to J2
Accurately measure medicine 1ml, with J2 suspension (2.5 times of the dilutions of 100 nematodes/ml), respectively at the survival condition of observing J2 behind 24h, the 48h, survival number, the death toll of statistics nematode, calculation correction lethality.With the clear water is contrast, and each is handled 3 times and repeats, promptly nematode stiff motionless be dead worm, be crooked wriggling state and be the work worm.Nematode corrected mortality (%)=(handling dead nematode rate-dead nematode rate of contrast)/(1-contrasts dead nematode rate) * 100
2.4 toxic effect to worm's ovum
Accurately measure soup 1ml, (2.5 times of the dilutions of 100 ovum/ml) at relative moisture 80-90%, are cultivated 12d under the temperature 24-26 ℃ of condition, are contrast with the clear water, and each is handled 3 times and repeats with suspension.Handle the every 3d investigation in back once, record nematode hatching number calculates nematode hatching inhibiting rate.
Nematode hatching inhibiting rate (%)=(1-handles nematode hatching quantity/contrast nematode hatching quantity) * 100
3. result and analysis
Table 1 soybean pod shell flavonoid extract is to Meloidogyne incognita J2 toxic effect
The variable concentrations soup sees Table 4 to Meloidogyne incognita J2 toxic effect after diluting 2.5 times with J2 suspension, handles in 24h, the 48h, and with the increase of experimental concentration, the nematicide effect increases gradually; Dilution is accompanied several below 800 times, handles 24h, 48h, and the corrected mortality of nematode all reaches more than 50%.Show that soup has very strong toxic effect to Meloidogyne incognita J2.
2.2.2 influence to the Meloidogyne incognita egg hatch
Table 2 soybean pod shell flavonoid extract is to the influence of Meloidogyne incognita egg hatch
After the variable concentrations soup dilutes 2.5 times with J2 suspension, to the influence (table 2) of Meloidogyne incognita egg hatch.In each processing time,, the inhibiting rate of the hatching of nematode worm's ovum is increased gradually with the increase of experimental concentration; Dilution is accompanied several 200 times, and inhibiting rate shows that greater than 50% soup has certain inhibitory action to the hatching of Meloidogyne incognita worm's ovum.
The effect experiment report of eelworm-killing activity land for growing field crops
1 purpose and meaning
By field control effectiveness test, the control efficiency of checking agricultural chemicals and to the crop safety evaluation, for the popularization of medicament provides theoretical foundation.
2 methods
2.1 the preparation of soup
By modern superfine powder technology raw material are pulverized, with the tuber of stemona, soybean pod shell, Chinese prickly ash, tobacco leaf in 10: 3: 2: 2 ratio is mixed, add 2%MF, 2.5%NNO%, 2% poly-second 4000 and 4% respectively and draw back that powder is made activating agent and 17% white carbon black is made carrier, be configured to wetting powder.
2.2 application method
Before planting, the tomato field planting adopt repeatedly dilution method that wetting powder 1.0kg (T1), 1.5kg (T2), 2.0kg (T3), 2.5kg (T4) are fully mixed thoroughly with thin dry ground, medicine soil amount 15.8kg; , 1.8% Avermectin EC (T5) waters planting pit or field planting ditch before the celery field planting, every cave amount of liquid medicine 250mL, direct field planting on the same day is then irritated root 1 time again behind the 15d, every cave amount of liquid medicine 250mL; Clear water contrast (CK) is respectively irritated root 1 time, every cave 250mL behind the field planting same day and 15d.Adopted the sampling of z font on the 15th, 30,45 day behind the medicine, the 1st time 30 strains are investigated in every sub-district, and 20 strains are investigated in each every sub-district later on, investigate disease plant and root system occurring degree respectively, calculate disease index and relative control effect.
What that give birth to according to root knot the state of an illness be divided into 5 grades [6]: 0 grade---root system is complete, no root knot; 1 grade---a small amount of root knot is arranged, account for below 25% of root system number; 2 grades---the root knot number accounts for 26%～50% of root system number; 3 grades---the root knot number accounts for 51%～75% of root system number; 4 grades---root knot spy is many and bigger, accounts for 76%～100% of root system number.Each treatment region plant plant height of growth interim survey is investigated the safety of test medicine to celery.Adopt the sampling of z font to survey during results and produce 20 strains of every sub-district.
3. interpretation of result
3.1 control efficiency
As seen from Table 1, after the medication to root-knot nematode all more than 80%, and with the increase of dosage, preventive effect strengthens.Removed behind the medicine 15 days, dosage be at the bottom of the processing control efficiency of 1.0kg outside 1.8% Avermectin, the control efficiency of other processing is all apparently higher than 1.8% Avermectin.
Table 1 medicament is to the control efficiency of root-knot nematode
3.2 influence to tomato growth
By table 2 as seen, medicament can promote plant strain growth, improves tomato yield, can make plant height increase 5.99%-20.21%, volume increase 6.64%-15.50%.And the effect of volume increase is apparently higher than 1.8% Avermectin.
Table 2 medicament is to the influence of tomato growth
| Handle | Plant height/cm | Increasing rate/% | Output/(kg.hm 2) | Increasing rate/% |
| T1 | 74.3 | 5.99 | 51431.25 | 6.64 |
| T2 | 78.2 | 11.55 | 5149.36 | 6.77 |
| T3 | 81.5 | 16.26 | 54783.9 | 13.59 |
| T4 | 84.3 | 20.21 | 55702.34 | 15.50 |
| T5 | 75.2 | 7.28 | 5145.96 | 6.68 |
| CK | 70.1 | - | 48226.44 | - |
In addition, in other experiment, find, use the effect of the former medicine of the medicinal plant tuber of stemona, be better than medicinal plant Radix Stemonae extract stemonine by the differential of modern superfine powder technology preparation.
Claims (10)
1. novel biological preparation for killing root-knot nematode comprises active ingredient tuber of stemona powder.
2. novel biological preparation for killing root-knot nematode as claimed in claim 1 is characterized in that: comprise active ingredient tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf are pressed powder 90-110.
3. novel biological preparation for killing root-knot nematode as claimed in claim 2, it is characterized in that: the active ingredient that comprises tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf press powder 90-110, activating agent MF9-11, NNO10-14, poly-second 4000 9-11, draws back powder 18-22.
4. novel biological preparation for killing root-knot nematode as claimed in claim 3, it is characterized in that: the active ingredient that comprises tuber of stemona powder 450-550 by weight, and inferior active ingredient soybean pod shell powder 137-167, zanthoxylum powder 90-110, tobacco leaf are pressed powder 90-110, activating agent MF powder 9-11, NNO powder 10-14, poly-second 4000 powder 9-11, draw back powder powder 18-22, the white carbon black powder 78-94 of carrier.
5. novel biological preparation for killing root-knot nematode as claimed in claim 4, it is characterized in that: the active ingredient that comprises tuber of stemona powder 480-520 by weight, and inferior active ingredient soybean pod shell powder 147-157, zanthoxylum powder 95-105, tobacco leaf are pressed powder 95-105, activating agent MF powder 9.5-10.5, NNO powder 11-13, poly-second 4000 powder 9.5-10.5, draw back powder powder 19-21, the white carbon black powder 83-89 of carrier.
6. novel biological preparation for killing root-knot nematode as claimed in claim 5, it is characterized in that: the active ingredient that comprises tuber of stemona powder 506 by weight, and inferior active ingredient soybean pod shell powder 152, zanthoxylum powder 101, tobacco leaf are pressed powder 101, activating agent MF powder 10, NNO powder 12, poly-second 4000 powder 10, draw back powder powder 20, the white carbon black powder 86 of carrier.
7. as the described novel biological preparation for killing root-knot nematode of arbitrary claim in the claim 1 to 6, it is characterized in that: described powder is the micro mist thinner than 500 orders.
8. novel biological preparation for killing root-knot nematode as claimed in claim 7 is characterized in that: said preparation is the wettable micro mist.
9. as the using method of the described novel biological preparation for killing root-knot nematode of arbitrary claim in the claim 1 to 8, it is characterized in that: dilute 200-1000 times of field and irritate root.
10. as the using method of the described novel biological preparation for killing root-knot nematode of arbitrary claim in the claim 1 to 8, it is characterized in that: directly spread fertilizer over the fields in the field.
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| CN2006101284650A CN101209059B (en) | 2006-12-26 | 2006-12-26 | Novel biological preparation for killing root-knot nematode and application method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422162B (en) * | 2008-12-09 | 2011-04-13 | 昆明线敌生物科技有限公司 | Plant source preparation for preventing and treating Meloidogyne incognita disease and preparation method thereof |
| CN102524309A (en) * | 2012-01-17 | 2012-07-04 | 山西大学 | Botanical nematicide |
| CN103907650A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Plant-derived pesticide as well as preparation method and application of plant-derived pesticide |
| CN105707142A (en) * | 2016-02-16 | 2016-06-29 | 哈尔滨凝昇科技有限公司 | Pesticide composition for preventing and treating soybean nematode worms and preparing method thereof |
| CN108605934A (en) * | 2018-04-20 | 2018-10-02 | 中国热带农业科学院热带生物技术研究所 | Application of two kinds of compounds in preparing nematicidal drug |
| CN108617647A (en) * | 2018-04-20 | 2018-10-09 | 中国热带农业科学院热带生物技术研究所 | Application of two kinds of compounds in preparing nematicidal drug |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134786A (en) * | 1995-07-04 | 1996-11-06 | 殷振鹏 | Multi-purpose, no-polution composite agricultural chemicals for plants and method for mfg. same |
| CN1546440A (en) * | 2003-11-28 | 2004-11-17 | 阎诚谦 | Composite plant insecticidal fertilizer and its production process |
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2006
- 2006-12-26 CN CN2006101284650A patent/CN101209059B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422162B (en) * | 2008-12-09 | 2011-04-13 | 昆明线敌生物科技有限公司 | Plant source preparation for preventing and treating Meloidogyne incognita disease and preparation method thereof |
| CN102524309A (en) * | 2012-01-17 | 2012-07-04 | 山西大学 | Botanical nematicide |
| CN103907650A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Plant-derived pesticide as well as preparation method and application of plant-derived pesticide |
| CN103907650B (en) * | 2014-04-04 | 2015-11-18 | 山东丰本生物科技股份有限公司 | Plant source type agricultural chemicals and preparation method thereof and application for one kind |
| CN105707142A (en) * | 2016-02-16 | 2016-06-29 | 哈尔滨凝昇科技有限公司 | Pesticide composition for preventing and treating soybean nematode worms and preparing method thereof |
| CN105707142B (en) * | 2016-02-16 | 2018-11-09 | 河南柏裕植物免疫科技有限公司 | A kind of pharmaceutical composition and preparation method thereof of prevention soybean nematode |
| CN108605934A (en) * | 2018-04-20 | 2018-10-02 | 中国热带农业科学院热带生物技术研究所 | Application of two kinds of compounds in preparing nematicidal drug |
| CN108617647A (en) * | 2018-04-20 | 2018-10-09 | 中国热带农业科学院热带生物技术研究所 | Application of two kinds of compounds in preparing nematicidal drug |
| CN108617647B (en) * | 2018-04-20 | 2020-11-10 | 中国热带农业科学院热带生物技术研究所 | Application of two compounds in preparation of nematocide |
| CN108605934B (en) * | 2018-04-20 | 2020-12-22 | 中国热带农业科学院热带生物技术研究所 | Application of two compounds in preparation of nematocide |
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