CN105660717A

CN105660717A - Organic water chestnut plant insecticide and application method thereof

Info

Publication number: CN105660717A
Application number: CN201610020597.5A
Authority: CN
Inventors: 郭兆武; 邵亚楠; 杨洁
Original assignee: Changsha University of Science and Technology
Current assignee: Changsha University of Science and Technology
Priority date: 2016-01-14
Filing date: 2016-01-14
Publication date: 2016-06-15
Anticipated expiration: 2036-01-14
Also published as: CN105660717B

Abstract

The invention discloses an organic water chestnut plant insecticide and an application method thereof. The plant insecticide is prepared by coriaria sinica fresh stalk, buddleia fresh stalk, thunder god vine fresh root bark and human urine. The weight proportion of the coriaria sinica fresh stalk, buddleia fresh stalk, thunder god vine fresh root bark and human urine is 2.0-3.0:1.0-2.0:1.5-2.5:3.0-4.0, the preferable proportion is 2.5:1.5:2.0:3.5, and the components are metered according to the weight proportion. The thunder god vine fresh root bark is an auxiliary agent, and the components have synergistic interaction effect. The plant insecticide has obvious effect for killing three main insects affecting organic water chestnut. The plant insecticide is diluted according to a ratio of 1.0:2.0 and 20-30 kg of the plant insecticide is diluted and sprayed for each 667 m<2> of a paddy field. The plant insecticide has the advantages that dosage form is stable, raw material source is abundant, usage is convenient for production, operation is simple, cost is low, no chemical pollution is generated, and organic water chestnut production requirement is completely met.

Description

A kind of organic water chestnut vegetable insecticide and using method thereof

Technical field

The invention belongs to agriculture practical new technology research and development and application; The present invention relates to a kind of research and development and production application method of organic water chestnut vegetable insecticide.

Background technology

Water chestnut (Eleocharisdulcis) monocot genus Cyperaceae, be perennial root herbaceous plant, oblateness, above point, smooth surface is glossy, aubergine or pitchy are grown in pond, bottle green stem is on the ground grown thickly, underground bulb can be edible. Water chestnut is because of perennial marsh sward basis, and the pasture root-like stock of crawling is elongated. End enlarges into oblate spherical, diameter 4cm, pitchy; The aerial stem of plant is cylindrical, up to 75cm, grows thickly, and not branch, hollow, tool tabula, surface smoothing, look green, and blade is degenerated, leaf sheath film matter, sheath mouth is oblique, easily comes off. One of spike, top is raw, upright, first fill cylindrical, light green. Spend number many or most; The wide obovate of scale, spiral or imbricate is arranged, and there is fine and closely woven vertical vertical bar line at back; 6 pieces, perianth, becomes bristle, the raw hook of upper tool; Stamen 2, filigree is elongated; Ovary is upper, and column cap 2 or 3 splits, dark brown. Pyrene is biconvex mirror shape, is about 2.5mm. 6～July of florescence. Sobole tip enlarges into bulb, oblate spherical, is dark chestnut shell palladium or purplish red after aging, have link 3～5 circle, and have short beak-like terminal bud and lateral bud, meat be white, quality is tender and crisp, succulence and sweet, both can be used as fruit, can be regarded as again vegetables, be the product in popular season, its meat is pure white, the sweet succulence of taste, clear and melodious good to eat, there is the good reputation of " underground snow pear ", northerner is referred to as " south of the River ginseng ", both can cook fruit and eat something rare, and can do again vegetables edible, be the product in popular season. The quality requirements of water chestnut: with individual large, clean, fresh, skin is thin, meat is thin, taste is sweet, sharp and clear, good without slag person matter.

Water chestnut is a kind of aquatic industrial crops of high-yield and high-efficiency, and there is cultivation widely various places, and along with planting site industry restructuring, its cultivated area has the gesture of continuous expansion. In recent years, water chestnut disease and pest was on the rise, and had become the large obstacle that development water chestnut produces.

The Major Pests of water chestnut has the white standing grain snout moth's larva of water chestnut, water chestnut borer, water chestnut aphid etc. The white standing grain snout moth's larva of water chestnut and water chestnut borer, all belong to Lepidoptera, Pyralidae, is the important pests of south China water chestnut. The white standing grain snout moth's larva of water chestnut claims again water chestnut white snout moth's larva, and water chestnut borer has another name called the yellow white standing grain snout moth's larva of water chestnut, and the two is all widely distributed, heavier with Zhejiang, Jiangsu, Anhui, Hunan and Jiangxi generation, is also distributed in addition the ground such as Beijing, Shandong, Henan, Hebei and Heilungkiang. Except water chestnut, also can endanger sugarcane, seat grass and Cyperaceae, grassy weed. The feature that causes harm is with the harm of larva moth stem. Water chestnut cladode diaphragm is eaten hollow, and the chlorisis variable color of stem top from top to bottom reddens, flavescence gradually, rots to stem stalk browning eventually, and severe patient complete stool is withered. The plant division that causes of being injured tillering stage reduces, and seedling is counted deficiency; It is withered that what the heading stage was injured cause stem stalk, and it is small and light that bulb becomes, and quality declines, and output falls sharply. The two plesiomorphism, yellow white standing grain snout moth's larva body is slightly large, adult wing expanse male worm 23-26mm, female worm 40-42mm, all white, only female moth stern squama clump sepia, male moth hind wing reverse side crineous. Ovum subcircular, ovum grain is piled into oval pieces of an egg, is covered with sepia fine hair. The long 15mm of larva mature larva body, yellow-white is grey slightly. Pupa cylindrical shape, initial stage milky, gradual change is faint yellow, compound eye brown. There is 4-5 generation in 1 year in this worm, generation overlap is tied thin cocoon with larva and survived the winter in water chestnut stem stalk. On the weeds such as near the seat grass of Cyperaceae, grass family and the pulse family of next spring being transferred to, amur foxtail, Chinese milk vetch or crop, take food and continue to grow, between May, pupate and sprout wings winter generation adult, moth fly to last year water chestnut be lost in the volunteer Oviposition in field. In Zhejiang, at the beginning of in year, overwinter generation and first comes across respectively 6 months to third generation moth appearance peak period, late July, late August, mid-October. 1-3 endangers then water chestnut seedling field and the field of planting for larva, with 2,3 generation generating capacity large. In the 4th generation, came across late September to early November, this in generation larva after slowly growing up, enter and survive the winter. Adult has green spawning habit, a little less than phototaxis, is not good at flying, and ovum fecund is in 6-7cm place, the nearly top of water chestnut cladode, and minority originates near Cyperaceae, grassy weed and crop, and piece produces, and mostly is stem one pieces of an egg. Newly hatched larvae has gregariousness, can have borer population bar to tens of in a cladode. Larva can weave silk sagging, the elsewhere that floats down on the wind, and 2-3 instar larvae can turn strain harm, and mature larva spins and pupates at basal part of stem.

Long 1.5～the 4.9mm of water chestnut aphid body, most about 2mm. Sometimes by wax powder, but lack wax disk(-sc). Feeler 6 saves, and minority 5 saves, rare 4 joints, and sensation ring shape, rare ellipse, base portion is often longer than in minor details end. Eye is large, and many facets, often have 3 outstanding facet optic tubercles. Beak minor details are short blunt to long point. Belly is greater than head and chest sum. The each joint of shirtfront and the belly knurl of often having chance with. The common tubulose of cornical, long is often greater than widely, and base portion is thick, and gradually thin to end, expand sometimes middle part or end, and top is often had chance with prominent, smooth surface or have watt line or end to have reticulate pattern, and rare have or less or many hairs, rare cornical ring-type or scarce. Cauda conical, finger-type, sword shape, triangle, pentagon, helmet shape are to semilune. Tailgate end circle. Epidermis is smooth, by reticulate pattern or wrinkle or the speckle that formed by aculea or particle. Sharp-pointed or the ultimate swelling of chaeta is head or fan-shaped. Common 6 joints of alatae feeler, the 3rd or 3 and 4 or 3～5 joints have secondary sensation circle. In fore wing, Maitong is often divided into 3, and minority is divided into 2. Hind wing has 2 of brachiums conventionally, and rare hind wing diminishes, and vein is degenerated. Vein is inlayed black surround sometimes. Water chestnut aphid is divided wing, aptery two types, body colour is black, if to become aphid or aphid are clustered in the leaves of plants back side, tender stem, growing point and take, suck the juice of plant with needle-like piercing mouth parts, cell is damaged, growth disequilibrium, blade is curling shrinkage to the back side, lobus cardiacus growth retardation, when serious, plant stops growing, and even complete stool is wilted withered. When aphid is caused harm, discharge large quantity of moisture and honeydew, be dropped in lower blade, cause that mycosis occurs, make Leaf Physiology function be subject to obstacle, reduce the accumulation of dry, reduce the commodity value of water chestnut.

At present the method for preventing and treating of water chestnut insect pest is mainly contained: 1. cultural control, as suit measures to local conditions seed selection and plantation pest-resistant cultivar; Enhance field management; The planting time of suiting measures to local conditions to regulate, rational close planting, prevents field closing; Run water layer well and particularly rest in the phase of pupating deep irrigation moderately and at the right moment, contribute to kill worm pupa; Apply fertilizers scientifically, increasing application phosphorus potassium fertilizer, improves plant resistance; After results, remove the invalid cane in field, reduce insect hibernacle, and remove water chestnut volunteer in overwinter generation pupa emergence front shovel, force down first generation insect population; In conjunction with pruning, residual flower, sick deadwood leaf that aphid perch or worm's ovum were hidden, thoroughly remove, and concentrates and burn. 2. biological control is mainly to utilize the natural enemy such as ladybug, Chrysopa to prevent and treat, or prevents and treats for No. six with BT30 gram or bacillus thruingensis. 3. physical control mainly contains lamp light trapping adult. 4. chemical prevention is mainly used chemical pesticide, as 3% meter Le Er granule, 98% Padan, Furadan, 20% Isofenphos methyl missible oil, 20% triazophos emulsifiable solution, 18% dimehypo, 90% trichlorphon, 20% triazophos emulsifiable solution, 20% Isofenphos methyl missible oil.

The above method of preventing and treating is all that effectively some even has remarkable result, as chemical prevention. But these methods of preventing and treating also have its obvious shortcoming, as: cultural control is time-consuming takes a lot of work, and in the situation that the blue or green strong labour power in current rural area is few, problem is more outstanding; Physical control is time-consuming taking a lot of work not only, can only be a kind of assisting in preventing and treating measure; Biological control is single-minded selective strong, can not prevent and treat comprehensively, as utilize the natural enemy such as ladybug, Chrysopa to prevent and treat, only effective to water chestnut aphid, prevents and treats for No. six with BT or bacillus thruingensis, only effective to water chestnut snout moth's larva; Although chemical prevention is respond well, pollute residual greatly, do not meet organic production requirement. And this organic water chestnut vegetable insecticide has overcome above these shortcomings, this organic water chestnut vegetable insecticide raw material is taken from the plant of free of contamination self-sow, its natural decomposition piece, residence time is short, easy to use, simple to operate, cost is low, insecticidal effect is remarkable, without any chemical contamination, meets the requirement of producing organic water chestnut completely.

Summary of the invention

Object of the present invention aims to provide a kind of organic water chestnut vegetable insecticide, and this pesticide is easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable. Another object of the present invention aims to provide the preferably using method of above-mentioned organic water chestnut vegetable insecticide. The object of the invention is to realize in the following manner:

Pesticide of the present invention is that the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people urinate brewed forming, and wherein the fresh fleshy root skin of thunder godvine, mainly as auxiliary agent, plays its insecticidal effect of promotion; People's urine is as brewed solvent. Inventor studies discovery, adds the fresh fleshy root skin of auxiliary agent thunder godvine can play good synergistic function.

What need particularly point out is, the fresh fleshy root skin of the fresh cane of Coriaria sinica, thunder godvine of this organic water chestnut vegetable insecticide, the fresh cane of building plum grass, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass are that self-sow forms, and do not use the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact.

The preparation method of this organic water chestnut vegetable insecticide is: the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people urinate except above-mentioned said all should selecting from the free of contamination areas of environmental condition such as soil, water, air, and soil, water body and fertilizer etc. also want heavy metal free to pollute. When preparation, first fresh Coriaria sinica cane, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass being put into a certain amount of people's urine in mass ratio soaks.

The weight mass ratio of four components of the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0. The most preferred weight mass ratio of the fresh cane of above four component Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine is 2.5:1.5:2.0:3.5. The fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine are pressed fresh weight mass ratio and are calculated, and people's urine calculates by the weight mass ratio of 1～30d. Four components of the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine mix in mass ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, plastic bag sealing, can preserve 2-3. While using this organic water chestnut vegetable insecticide to kill water chestnut insect, the thin water layer that water 2～5cm is dark need be stayed in field. Desinsection dosage, generally uses filtrate 20～30kg.667m^-2Press 1.0:2.0 dilution spraying, can kill preferably water chestnut insect. This pesticide is produced upper easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable, and the shelf-life is longer, packs with plastic bag sealing, can carry out batch production production, forms product.

The present invention selects the fresh cane of Coriaria sinica, the building plum fresh cane of grass and the fresh fleshy root skin of auxiliary agent thunder godvine, and brewed medium people urinates, and has adopted the compatibility of suitable quality, is mixed with commercial vegetable insecticide, and it has significant insecticidal effect. This organic water chestnut vegetable insecticide is easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable, this be other physical measure cannot be than. It is below the comparative test of its insecticidal effect.

Organic water chestnut vegetable insecticide of the present invention has no the report of like product at present, the present invention's result of the test that to be inventor draw through experimental study for many years and production practices, and be verified in the production of long period, effect is better. And the formula of the present invention content that also possesses skills is higher, and formulation is more stable, raw material sources are abundant, easy and simple to handle, as are developed to commodity, concerning producing organic water chestnut, and low price. Easy to use aborning, insecticidal effect is remarkable, and use cost is low, is the vegetable insecticide of a kind of good organic water chestnut of production. Organic water chestnut plant insecticide agent prescription can carry out batch production production, and it develops not only will increase a kind of novel organic water chestnut vegetable insecticide on market, also will provide a new solution route for the problem of producing at present organic water chestnut deinsectization poor effect.

Compound method of the present invention is: when preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine mix by quality weight ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, plastic bag sealing, can preserve 2-3.

Using method of the present invention is: while using this organic water chestnut vegetable insecticide desinsection, the thin water layer that water 2～5cm is dark need be stayed in field. Desinsection dosage, generally uses filtrate 20～30kg.667m^-2Press 1.0:2.0 dilution spraying, can kill preferably water chestnut insect. Be typically chosen in before at 10 in fine day morning or spray at dusk, after spraying, will ensure and in 15～20h, do not rain or manually spray, if rained in 15～20h or carried out artificial sprinkling irrigation, need again spray. Select larva or nymph before 3 ages to spray medicine, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and droplet is as far as possible fine, not repeat spraying, be limited not form the globule on plant, safe defence will strictly be carried out human body defence and field operation by chemical pesticide operational procedure. After spray medicine, in 30d, the mixed battalion of breeding can not be carried out in field. Test detects and shows, use this organic water chestnut vegetable insecticide, the organic water chestnut of commodity of producing thus detects, and the major function composition of its vegetable insecticide does not all detect.

Be below to invent artificially to realize object of the present invention, constantly select and select the applicable raw material of organic water chestnut plant insecticide agent prescription of the present invention and the comparative test of the insecticidal effect that relevant proportioning is done thereof by test.

One pack system test

One pack system test is mainly that each component of qualitative determination is to killing the single effect test of 3 kinds of organic water chestnut insects.

Materials and methods

1.1.1 test material

Test is selected organic water chestnut is produced to the 3 kinds of serious primary pests of causing harm: the white standing grain snout moth's larva of water chestnut, water chestnut borer and water chestnut aphid. Test by single dose is: the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people urinate four components.

Test method

The preparation of test material: the fresh cane of material Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass are that self-sow forms, and do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact. When preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio of the fresh cane of Coriaria sinica and people urine is 2.5::3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover to soak between 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years.

The quality weight ratio of the fresh cane of Coriaria sinica and people urine is 2.5:3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover to soak between 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. The fresh cane of building plum grass is 1.5:3.5 with the quality weight ratio of people's urine, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. The quality weight ratio of the fresh fleshy root skin of thunder godvine and people urine is 2.0:3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover to soak between 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. People's urine, with people's urine of 1～30d, the large Plastic Drum of 25～50L is added a cover to deposit between 30d(storage period and is not needed to seal). After 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. When field test, the thin water layer that water 2～5cm is dark is stayed in field, and separate with fly net each community. Desinsection dosage, with filtrate 20～30kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in and sprayed in the past at 10 in fine day morning, spray in rear guarantee 15h and do not rain, also manually do not spray. Contrast chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h, sprays in rear guarantee 15h and does not rain, and does not also manually spray. Test effect is mainly with its 3 kinds of primary pests: the percentage of the white standing grain snout moth's larva of water chestnut, water chestnut borer and the water chestnut aphid death rate is recently counted and statistical analysis. When the larva of spraying time before 3 ages or nymph, carry out, water chestnut borer will carry out in the time that ovum is hatched.

Field test: test arranges 5 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 10m²Field area, respectively processes random alignment. Each processing is numbered processing 1-5 and CK1.

Pot experiment: test arranges 5 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 1 basin, respectively processes random alignment. Each processing is numbered processing 6-10 and CK2.

Each disposition is as follows:

Process 1---the quality weight ratio of the fresh cane of Coriaria sinica and people urine is 2.5::3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 2---the fresh cane of building plum grass is 1.5:3.5 with the quality weight ratio of people's urine, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 3---the quality weight ratio of the fresh fleshy root skin of thunder godvine and people urine is 2.0:3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 4---with people's urine of 1～30d, the large Plastic Drum of 25～50L is added a cover to deposit between 30d(storage period and is not needed to seal). After 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m by this urine^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 5---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK1)---water spray, viewing test effect after 24h. Process 6---the quality weight ratio of the fresh cane of Coriaria sinica and people urine is 2.5::3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 7---the fresh cane of building plum grass is 1.5:3.5 with the quality weight ratio of people's urine, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 8---the quality weight ratio of the fresh fleshy root skin of thunder godvine and people urine is 2.0:3.5, with the people of 1～30d urinate the large Plastic Drum of 25～50L add a cover between immersion 30d(soak period do not need to seal), after 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m with this filtrate^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 9---with people's urine of 1～30d, the large Plastic Drum of 25～50L is added a cover to deposit between 30d(storage period and is not needed to seal). After 30d, by 3 layers of filtered through gauze, obtain its filtrate. Plastic bag sealing is preserved 3 years. Press 20kg.667m by this urine^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 10---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK2)---water spray, viewing test effect after 24h.

Statistical analysis

Test data is adopted to SAS(6.12) carry out statistical analysis. Result of the test shows (table 1), processes 1-3,5,6-8,10 the white standing grain snout moth's larva of the water chestnut death rate is significantly higher than other processing and CK1, CK2, processes 5, the white standing grain snout moth's larva death rate of processing 10 is significantly higher than other processing and CK1, CK2. More than explanation, the white standing grain snout moth's larva death rate of the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine is all significantly higher than people's urine and CK1, CK2, but significantly lower than processing 5, the processing 10 of chemical pesticide DDVP; The people of field test urinates and processes with the white standing grain snout moth's larva death rate of CK without significant difference; The people of pot experiment urinates and processes with the white standing grain snout moth's larva death rate of CK also without significant difference.

The test of table 1. one pack system---The death rate (unit: %) of three kinds of main water chestnut insects

Process	The white standing grain snout moth's larva of water chestnut	Water chestnut borer	Water chestnut aphid
				Process 1	42.64±3.64bc	38.25±6.12d	67.23±0.93d
Process 2	37.92±1.80c	41.65±3.54c	38.20±8.61g
				Process 3	38.21±2.58c	52.50±0.86b	51.09±2.19f
Process 4	1.54±0.15de	2.93±1.24f	1.64±0.25h
				Process 5	92.56±5.37a	82.61±6.18a	92.16±3.07a
CK1	2.57±0.62d	1.35±0.30f	0.94±0.31h
				Process 6	53.60±1.36b	51.81±4.17b	74.28±4.20c
Process 7	38.61±4.28c	31.26±2.61e	59.14±3.47e
				Process 8	43.28±1.64bc	39.50±1.50cd	69.57±0.67cd
Process 9	0.95±0.13e	1.27±0.43f	1.64±0.22h
				Process 10	96.56±6.80a	81.67±7.61a	97.03±5.82b
CK2	0.86±0.24e	2.61±1.16f	1.54±0.17h

Note: lowercase is 0.05 level, the different letter representation significant differences of same row, lower same.

Process 1-3,5,6-8,10 the water chestnut borer death rate is significantly higher than other processing and CK1, CK2, the borer death rate of processing 5, processing 10 is significantly higher than other processing and CK1, CK2, and the borer death rate of processing 4, processing 9 and CK1, CK2 are without significant difference (table 1). More than explanation, the borer death rate of the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine is all significantly higher than people's urine and CK1, CK2, but significantly lower than processing 5, the processing 10 of chemical pesticide DDVP; The potted plant borer death rate of urinating processing and CK with people field test is without significant difference.

Process 1-3,5,6-8,10 the water chestnut aphid death rate is significantly higher than other processing and CK1, CK2, the aphid death rate of processing 5, processing 10 is significantly higher than other processing and CK1, CK2, and the aphid death rate of processing 4, processing 9 and CK1, CK2 are without significant difference (table 1). More than explanation, the aphid death rate of the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine is all significantly higher than people's urine and CK1, CK2, but significantly lower than processing 5, the processing 10 of chemical pesticide DDVP; The potted plant aphid death rate of urinating processing and CK with people field test is without significant difference.

All show by above one pack system field experiment and pot experiment, the death rate of three kinds of main water chestnut insects of the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass is all significantly higher than people and urinates processing and CK1, CK2, but significantly lower than the processing of chemical pesticide DDVP. People urinates and processes with the death rate of the CK1, the CK2 that spray water without significant difference.

Compatibility test

Compatibility test is mainly that qualitative and quantitative is measured each ingredients to killing the composite effect test of 3 kinds of organic water chestnut primary pests.

Materials and methods

2.1.1 test material

Test is selected organic water chestnut is produced to the 3 kinds of serious primary pests of causing harm: the white standing grain snout moth's larva of water chestnut, water chestnut borer and water chestnut aphid. Test is that the careless fresh cane of the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, building plum, people urinate four components by ingredients.

Test method

The preparation of test material: the fresh cane of material Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass are that self-sow forms, and do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact. When preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine mix by quality weight ratio ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, plastic bag sealing, uses pesticide stoste taking the vegetable insecticide of preserving 3 years as testing. When field test, the thin water layer that water 2～5cm is dark is stayed in field, and separate with fly net each community. Desinsection dosage, with filtrate 20～30kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in and sprayed in the past at 10 in fine day morning, spray in rear guarantee 15h and do not rain, also manually do not spray. Contrast chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h, sprays in rear guarantee 15h and does not rain, and does not also manually spray. Test effect is mainly with its 3 kinds of primary pests: the percentage of the white standing grain snout moth's larva of water chestnut, water chestnut borer and the water chestnut aphid death rate is recently counted and statistical analysis. When the larva of spraying time before 3 ages or nymph, carry out, water chestnut borer will carry out in the time that ovum is hatched.

Field test: test arranges 4 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 10m²Field area, respectively processes random alignment. Each processing is numbered processing 1-4 and CK1.

Pot experiment: test arranges 4 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 1 basin, respectively processes random alignment. Each processing is numbered processing 5-8 and CK2.

Each disposition is as follows:

Process 1---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0:1.0:1.5:3.0. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 2---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 3---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 3.0:2.0:2.5:4.0. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 4---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK1)---water spray, viewing test effect after 24h. Process 5---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0:1.0:1.5:3.0. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 6---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 7---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 3.0:2.0:2.5:4.0. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 8---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK2)---water spray, viewing test effect after 24h.

Statistical analysis

Test data is adopted to SAS(6.12) carry out statistical analysis. Result of the test shows (table 2), the each white standing grain snout moth's larva of water chestnut death rate of processing is significantly higher than CK1, CK2, processing 8 the white standing grain snout moth's larva of the water chestnut death rate is significantly higher than and processes 1-2,5,7 and CK1, CK2, the white standing grain snout moth's larva death rate of processing 2-3 is significantly higher than processes 1, process, the death rate of 6-7 is significantly higher than again processes 5. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0, presses 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the killing action of the effect dialogue standing grain snout moth's larva of the field after 24h and pot experiment is remarkable, and uses chemical pesticide DDVP, spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect after 24h is basic identical. Wherein, the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate pot experiment effect that the quality weight ratio of four components is 2.5:1.5:2.0:3.5 and are significantly better than the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people and urinate the test effect that the quality weight ratio of four components is 2.0:1.0:1.5:3.0, but to urinate the compatibility effect that the quality weight ratio of four components is 3.0:2.0:2.5:4.0 basic identical with the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people. Consider in conjunction with insecticidal effect from economic benefit, it is the better ingredients of killing white standing grain snout moth's larva that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate the compatibility that the quality weight ratio of four components is 2.5:1.5:2.0:3.5.

The test of table 2. compatibility---The death rate (unit: %) of three kinds of main water chestnut insects

Process	The white standing grain snout moth's larva of water chestnut	Water chestnut borer	Water chestnut aphid
				Process 1	82.48±3.52d	80.17±1.67c	85.73±3.50d
Process 2	91.26±1.65bc	85.09±3.19bc	96.61±1.27ab
				Process 3	93.54±2.82ab	86.27±0.76b	98.20±0.93a
Process 4	94.06±0.83ab	85.61±3.28bc	97.53±4.67a
				CK1	2.17±1.08e	0.83±0.29d	2.67±0.34e
Process 5	90.65±5.71c	82.51±2.80c	90.64±4.26c
				Process 6	93.40±1.64ab	84.17±4.17bc	96.37±1.73ab
Process 7	92.86±3.46b	87.34±2.06ab	95.18±0.79b
				Process 8	96.34±2.81a	89.26±1.38a	98.16±5.60a
CK2	1.67±0.42e	0.91±0.31d	3.08±0.43e

Each water chestnut borer death rate of processing is significantly higher than CK1, CK2, and the borer death rate of processing 8 is significantly higher than processes 1-4,5-6 and CK1, CK2, and the borer death rate of processing 2-3,6-7 is obviously better than again processing 1,5. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0, presses 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of pot experiment are remarkable to the killing action of borer, and use chemical pesticide DDVP, spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect after 24h is basic identical. Wherein, the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate pot experiment effect that the quality weight ratio of four components is 2.5:1.5:2.0:3.5 and are significantly better than the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people and urinate the test effect that the quality weight ratio of four components is 2.0:1.0:1.5:3.0, but to urinate the compatibility effect that the quality weight ratio of four components is 3.0:2.0:2.5:4.0 basic identical with the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people. Consider in conjunction with insecticidal effect from economic benefit, it is the better ingredients of killing borer that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate the compatibility that the quality weight ratio of four components is 2.5:1.5:2.0:3.5.

Each water chestnut aphid death rate of processing is significantly higher than CK1, CK2, processing 3-4,8 the aphid death rate is significantly higher than and processes 1,5 and CK1, CK2, the aphid death rate of processing 2-3,6-7 is significantly higher than again processes 1,5, processes the aphid death rate of 2-3,6-7 without significant difference. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0, presses 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of pot experiment are remarkable to the killing action of aphid, and use chemical pesticide DDVP, spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect after 24h is basic identical. Wherein, the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate field that the quality weight ratio of four components is 2.5:1.5:2.0:3.5 and pot experiment effect and are significantly better than the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people and urinate the test effect that the quality weight ratio of four components is 2.0:1.0:1.5:3.0, but to urinate the compatibility effect that the quality weight ratio of four components is 3.0:2.0:2.5:4.0 basic identical with the fresh cane of Coriaria sinica, the careless fresh cane of building plum, the fresh fleshy root skin of thunder godvine, people. Consider in conjunction with insecticidal effect from economic benefit, it is the better ingredients of killing aphid that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate the compatibility that the quality weight ratio of four components is 2.5:1.5:2.0:3.5.

More than test also shows, the death rate (table 2) that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate three kinds of main water chestnut insects of four component compatibilities tests is significantly higher than the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinates the death rates (table 1) of three kinds of main water chestnut insects of four component single doses tests. Show thus, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four component compatibilities and have good synergistic function.

Dosetest

Dosetest is mainly that qualitative and quantitative is measured each using dosage to killing the effect test of 3 kinds of organic water chestnut primary pests.

Materials and methods

3.1.1 test material

Test method

The preparation of test material: the fresh cane of material Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass are that self-sow forms, and do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact. When preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine mix by quality weight ratio ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, plastic bag sealing, uses pesticide stoste taking the vegetable insecticide of preserving 3 years as testing. When field test, the thin water layer that water 2～5cm is dark is stayed in field, and separate with fly net each community. Desinsection dosage, with filtrate 20～30kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in and sprayed in the past at 10 in fine day morning, spray in rear guarantee 15h and do not rain, also manually do not spray. Contrast chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h, sprays in rear guarantee 15h and does not rain, and does not also manually spray. Test effect is mainly with its 3 kinds of primary pests: the percentage of the white standing grain snout moth's larva of water chestnut, water chestnut borer and the water chestnut aphid death rate is recently counted and statistical analysis. When the larva of spraying time before 3 ages or nymph, carry out, water chestnut borer will carry out in the time that ovum is hatched.

Each disposition is as follows:

Process 1---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 2---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 3---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 4---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK1)---water spray, viewing test effect after 24h. Process 5---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 6---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 7---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 8---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK2)---water spray, viewing test effect after 24h.

Statistical analysis

Test data is adopted to SAS(6.12) carry out statistical analysis. Result of the test shows (table 3), and the white standing grain snout moth's larva of the water chestnut death rate of processing 1-4,5-8 is all significantly higher than CK1, CK2. The white standing grain snout moth's larva death rate of processing 2-4,5-8 is significantly higher than processing 1 and CK1, CK2, but processes between 2-4,6-8 without significant difference; Process 2 and be significantly higher than processing 1; Process 6 and be obviously better than processing 5. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, presses 25-30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the killing action of the pot experiment effect dialogue standing grain snout moth's larva after 24h is remarkable, and use chemical pesticide DDVP, spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect of observing after 24h is without significant difference. Wherein, compatibility liquid stoste is pressed 25kg.667m^-2The field test effect of dosage is significantly better than compatibility liquid stoste by 20kg.667m^-2The test effect of dosage, but press 30kg.667m with compatibility liquid stoste^-2The field of dosage and pot experiment effect are without significant difference. Consider in conjunction with insecticidal effect from economic benefit, compatibility liquid stoste is pressed 25kg.667m^-2Dosage be the better dosage of killing white standing grain snout moth's larva.

Table 3. dosetest---The death rate (unit: %) of three kinds of main water chestnut insects

Process	The white standing grain snout moth's larva of water chestnut	Water chestnut borer	Water chestnut aphid
				Process 1	91.26±2.14c	85.09±2.61bc	96.61±1.38b
Process 2	93.30±0.93b	87.30±1.67b	98.17±3.37a
				Process 3	95.18±3.16ab	88.29±2.54ab	98.69±0.91a
Process 4	94.06±1.28ab	85.61±1.43bc	97.53±2.14ab
				CK1	2.17±0.35d	0.83±0.29d	2.67±1.17c
Process 5	93.40±4.29b	84.17±4.61c	96.37±4.90b
				Process 6	95.01±2.81ab	89.53±0.81a	97.53±2.53ab
Process 7	96.17±0.91a	90.17±1.12a	99.24±4.08a
				Process 8	96.34±1.61a	89.26±3.70a	98.16±0.83a
CK2	1.67±0.39d	0.91±0.42d	3.08±1.15c

The water chestnut borer death rate of processing 1-4,5-8 is all significantly higher than CK1, CK2. Process between 1-4 without significant difference,, process 6-8 be significantly higher than process 5(table 3); Process 2,6 and be obviously better than processing 1,5. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, presses 25-30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of pot experiment are remarkable to the killing action of water chestnut borer, and spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect of observing after 24h is without significant difference. Wherein, compatibility liquid stoste is pressed 25kg.667m^-2The pot experiment effect of dosage is significantly better than compatibility liquid stoste by 20kg.667m^-2The pot experiment effect of dosage, but press 30kg.667m with compatibility liquid stoste^-2Field and the pot test effect of dosage are basic identical. Consider in conjunction with insecticidal effect from economic benefit, compatibility liquid stoste is pressed 25kg.667m^-2Dosage be the better dosage of killing water chestnut borer.

The water chestnut aphid death rate of processing 1-4,5-8 is all significantly higher than CK1, CK2. Between processing 2-4, without significant difference, process between 6-8 without significant difference (table 3); Process 2,6 and be obviously better than processing 1,5. More than explanation, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, presses 25-30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of pot experiment are remarkable to the killing action of water chestnut aphid, and spray by 80% DDT EC l000 times of liquid, every 667m²The dosage of dilution 50kg for water chestnut field, the test effect of observing after 24h is without significant difference. Wherein, compatibility liquid stoste is pressed 25kg.667m^-2The field test effect of dosage is significantly better than compatibility liquid stoste by 20kg.667m^-2The test effect of dosage, but press 30kg.667m with compatibility liquid stoste^-2The field of dosage and pot test effect are without significant difference. Consider in conjunction with insecticidal effect from economic benefit, compatibility liquid stoste is pressed 25kg.667m^-2Dosage be the better dosage of killing water chestnut aphid.

More than test shows, the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, presses 25kg.667m by this compatibility liquid stoste^-2The field of dosage 1.0:2.0 dilution spraying and the pot experiment effect of killing 3 kinds of water chestnut primary pests be significantly better than 20kg.667m^-2The effect of dosage spraying, but and 30kg.667m^-2The effect of dosage spraying is basic identical. Consider 25kg.667m in conjunction with insecticidal effect from economic benefit^-2Dosage spraying is better dosage.

More than test also shows, the insecticidal effect that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people the urinate four component compatibility dosetests i.e. death rate of 3 kinds of water chestnut primary pests (table 2-3) is significantly higher than the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people and urinates the death rate (table 1) of four component single doses tests. Show thus, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four component compatibilities and have good synergistic function.

Auxiliary agent test

Auxiliary agent test is mainly qualitative and quantitative mensuration auxiliary agent is killed 3 kinds of organic water chestnut primary pests synergistic function to whole ingredients.

Materials and methods

4.1.1 test material

Test is selected organic water chestnut is produced to the 3 kinds of serious primary pests of causing harm: the white standing grain snout moth's larva of water chestnut, water chestnut borer and water chestnut aphid. Test is that the careless fresh cane of the fresh cane of Coriaria sinica, building plum, the fresh fleshy root skin of thunder godvine, people are urinated four components by ingredients. Wherein the fresh fleshy root skin of thunder godvine is auxiliary agent.

Test method

The preparation of test material: the fresh cane of material Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass are that self-sow forms, and do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact. When preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine mix by quality weight ratio ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, plastic bag sealing, uses pesticide stoste taking the vegetable insecticide of preserving 3 years as testing. When field test, the thin water layer that water 2～5cm is dark is stayed in field, and separate with fly net each community. Desinsection dosage, uses filtrate 25kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in and sprayed in the past at 10 in fine day morning, spray in rear guarantee 15h and do not rain, also manually do not spray. Contrast chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h, sprays in rear guarantee 15h and does not rain, and does not also manually spray. Test effect is mainly with its 3 kinds of primary pests: the percentage of the white standing grain snout moth's larva of water chestnut, water chestnut borer and the water chestnut aphid death rate is recently counted and statistical analysis. When the larva of spraying time before 3 ages or nymph, carry out, water chestnut borer will carry out in the time that ovum is hatched.

Field test: test arranges 3 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 10m²Field area, respectively processes random alignment. Each processing is numbered processing 1-3 and CK1.

Pot experiment: test arranges 3 and processes 1 contrast, 3 repetitions of each processing (comprising CK), each repetition 1 basin, respectively processes random alignment. Each processing is numbered processing 4-6 and CK2.

Each disposition is as follows:

Process 1---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 2---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, people urinate three components is 2.5:1.5:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 3---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK1)---water spray, viewing test effect after 24h. Process 4---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 5---the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, people urinate three components is 2.5:1.5:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. Viewing test effect after 24h. Process 6---use chemical pesticide DDVP, by 80% DDT EC l000 times of liquid spraying, every 667m²The dosage of dilution 50kg for water chestnut field. Viewing test effect after 24h. Contrast (CK2)---water spray, viewing test effect after 24h.

Statistical analysis

Test data is adopted to SAS(6.12) carry out statistical analysis. Result of the test shows (table 4), processes 2,5 the white standing grain snout moth's larva of the water chestnut death rate significantly lower than processing 1,3,4,6, but is significantly higher than CK1, CK2. More than explanation, the quality weight ratio of urinating three components without the fresh cane of compatibility Coriaria sinica of auxiliary agent, the fresh cane of building plum grass, people is 2.5:1.5:3.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the quality weight ratio that the death rate of the effect dialogue standing grain snout moth's larva of the field after 24h and pot experiment is significantly urinated four components lower than the fresh cane of the fresh cane of compatibility Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, the people that have auxiliary agent is 2.5:1.5:2.0:3.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the death rate of the effect dialogue standing grain snout moth's larva of the field after 24h and pot experiment; Also significantly lower than with chemical pesticide by 80% DDT EC l000 times liquid every 667m that sprays²Field after the dosage 24h of dilution 50kg and the death rate of pot experiment for water chestnut field.

The test of table 4. auxiliary agent---The death rate (unit: %) of three kinds of main water chestnut insects

Process	The white standing grain snout moth's larva of water chestnut	Water chestnut borer	Water chestnut aphid
				Process 1	93.30±1.67b	87.30±2.94ab	98.17±2.93a
Process 2	85.35±3.41c	72.96±1.61c	84.31±0.61c
				Process 3	94.06±0.94ab	85.61±5.31b	97.53±1.85ab
CK1	2.17±0.28e	0.83±0.37d	2.67±0.49d
				Process 4	95.01±4.63a	89.53±3.60a	97.53±4.13ab
Process 5	80.91±1.80d	74.28±4.07c	86.09±0.50b
				Process 6	96.34±3.77a	89.26±0.81a	98.16±3.74a
CK2	1.67±0.27e	0.91±0.22d	3.08±2.17d

Process 2,5 the water chestnut borer death rate significantly lower than processing 1,3,4,6, but be significantly higher than CK1, CK2(table 4). More than explanation, the quality weight ratio of urinating three components without the fresh cane of compatibility Coriaria sinica of auxiliary agent, the fresh cane of building plum grass, people is 2.5:1.5:3.5, presses 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, field after 24h and the effect of pot experiment to the killing action of borer significantly lower than the compatibility that has auxiliary agent: the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of the pot experiment death rate of killing to borer; Also significantly lower than with chemical pesticide by 80% DDT EC l000 times liquid every 667m that sprays²Field and the pot experiment death rate after the dosage 24h of dilution 50kg for water chestnut field.

Process 2,5 the water chestnut aphid death rate significantly lower than processing 1,3,4,6, but be significantly higher than CK1, CK2(table 4). More than explanation, the compatibility without auxiliary agent: the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, people urinate three components is 2.5:1.5:3.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, field after 24h and the effect of pot experiment are killed the death rate significantly lower than the compatibility that has auxiliary agent to aphid: the quality weight ratio that the fresh cane of the fresh cane of Coriaria sinica, building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying, the field after 24h and the effect of the pot experiment death rate of killing to aphid; Also significantly lower than with chemical pesticide by 80% DDT EC l000 times liquid every 667m that sprays²Field and the pot experiment death rate after the dosage 24h of dilution 50kg for water chestnut field.

More than test shows (table 4), and the difference on effect of adding assistant and not adding assistant is remarkable, and the effect of adding assistant is significantly better than the not effect of adding assistant, and its effect of adding assistant does not significantly reduce. Illustrate that the fresh fleshy root skin of auxiliary agent thunder godvine has significant collaborative and synergistic effect in this compatibility.

Test also shows (table 1-4), and the effect of ingredients is the doubly left and right of 1.5-2 of each single dose effect. So the effect after compatibility is not the addition of simple each single dose effect. Be tested to compatibility test again to auxiliary agent test (table 1-4) from single dose, result shows, the fresh cane of compatibility Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate compatibility liquid stoste that the quality weight ratio of four components is 2.5:1.5:2.0:3.5 by 25kg.667m^-2The ingredients of dosage 1.0:2.0 dilution spraying, consider it is better selection from economic benefit in conjunction with insecticidal effect. Test shows, sprays the main component coriarine that do not detect the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass in the commodity water chestnut of rear 30d, robinin, wilfordine etc. with the compatibility agent that the fresh cane of Coriaria sinica, the fresh fleshy root skin of thunder godvine, the fresh cane of building plum grass, people urinate four components.

Coriaria sinica (CoriarianepalensisWall.) belong to Sapindales, Coriariaceae shrub, its leaf is to life, and papery is thin keratin extremely, ellipse, raceme is born on biennial branch, petal meat, keel-shaped. The first leaf of male inflorescence is open, spends more intensive; Sepal is avette, and edge is translucent, top tool tasselled shape serration; When filigree flower, extend; There is sterile gynoecium. Female inflorescence and leaf go out together, aubergine. Berry shape achene, when ripe by red purpling black, the florescence 3-4 month, the fruit phase 5-6 month. Be distributed in the ground such as Yunnan, Guizhou, Sichuan, Hubei, Hunan, Shaanxi, Gansu. Fruit can be carried alcohol. Seed oil expression can be painted and ink. Cauline leaf can be carried tannin extract. Complete stool contains coriarine, tutin etc., and its toxic component is coriamyrtin, tutin etc.

Lou Meicao (BuddlejalindleyanaFortune) Loganiaceae shrub, stem skin brown; Sprig tool four ribs, slightly narrow wing on rib; Below sprout, blade, petiole, inflorescence, bract and squamella all close by starlike down and glandular hairs. Leaf is to life, and the leaf on rudiment branch is alternate or closely verticillate, and blade is membranous, and avette, oval to oval shape lanceolar, base portion often has place to deposit calyx; Seed is filbert, little, aptery. The florescence 4-10 month, really April in August phase to next year. Originate in roadside, mountain region, the river bank bushess such as Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan or border. Complete stool is containing multiple flavonoids such as linariin, robinins.

Thunder godvine (TripterygiumwilfordiiHook.f.), Celastraceae, tripterygium plant, liana shrub, main product is in China, Korea, Japan, China is mainly distributed in various places and southwest on the south the Yangtze river basin, main product in Fujian, the ground such as Zhejiang, Hubei, Hunan, Anhui, Henan. Be born in in the shade how wet hillside, mountain valley, small stream limit bushes. The hillside that happiness is comparatively shady and cool, with slant acidity, deep sand soil or the most suitable growth of yellow soil of soil layer. Bitter, pungent, cool in nature, poison greatly. Thunder God Calamus liana shrub, high 1-3 rice, sprig brownish red, has the thin rib of 4-6 root, by Mi Mao and fine and closely woven hole skin. Elliptic leaf shape, the ellipse of falling ovum, rectangular ellipse or avette, long 4-7.5 centimetre, wide 3-4 centimetre, the anxious point of tip or short gradually point, the wealthy wedge shape of base portion or circle, there is serration at edge, lateral vein 4-7 couple, reach slightly go up after leaf margin curved; The long 5-8 millimeter of petiole, close by rust hair. Circular cone cyme is narrower, long 5-7 centimetre, and wide 3-4 centimetre, has 3-5 branch conventionally. The oval shape of samara, long 1-1.5 centimetre, diameter 1-1.2 centimetre, seed buttress shaft shape, reaches 10 millimeters. Thunder godvine bitter, pungent, cool in nature, poison greatly, has clearing heat and detoxicating, dispelling wind and removing obstruction in the meridians, relaxes the muscles and stimulate the blood circulation, the effect such as swelling and pain relieving, desinsection hemostasis, returns liver, kidney channel, and effect is dispelled rheumatism, is removed obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection. The traditional Chinese medical science is used for the treatment of lepra reaction, rheumatoid arthritis, pulmonary tuberculosis and other chronic lung diseases etc. The effects such as that thunder godvine has is antitumor, anti-inflammatory, but it is very toxic, so the application of thunder godvine in medical treatment is restricted always. Tripterygium root mainly contains wilfordine, celastrus orbiculatus benzyl acid amides, Triptolide etc.

Detailed description of the invention

Following embodiment and embodiment are intended to further illustrate the present invention, instead of limitation of the present invention.

Embodiment 1:

The preparation of material: the fresh cane of material Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine are that self-sow forms, and do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic water chestnut of impact. When preparation, first earth, water on fresh Coriaria sinica cane, the building plum fresh cane of grass and the fresh fleshy root skin of thunder godvine are shaked off, do not needed washing. Thereafter, the quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine mix by quality weight ratio, the large Plastic Drum of 25～50L is added a cover to soak between 20～40d(soak period and is not needed to seal), after 20～40d by 3～4 layers of filtered through gauze, its filtrate is this organic water chestnut vegetable insecticide, and plastic bag sealing can be preserved 2～3 years.

While being chosen in larva before 3 ages of 3 kinds of organic water chestnuts primary pest or nymph service time, spray medicine, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and also can carry out early prevention. When use, the thin water layer that water 2～5cm is dark is stayed in field, uses filtrate 20kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in before at 10 in fine day morning or spray at dusk, spraying in rear guarantee 15～20h and do not rain, also manually not spraying, if rained in 15～20h or carried out artificial sprinkling irrigation, need again spray. Larva or nymph before 3 ages of spraying time are killed, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and droplet is as far as possible fine, not repeat spraying, be limited not form the globule on plant, safe defence will strictly be carried out human body defence and field operation by chemical pesticide operational procedure. After spray medicine, in 30d, the mixed battalion of breeding can not be carried out in field.

The quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 20kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. After 24h, the insecticidal effect in organic water chestnut field is: the white standing grain snout moth's larva of the water chestnut death rate 91.26%, the water chestnut borer death rate 85.09%, the water chestnut aphid death rate 96.61%, only differ respectively 2.80%, 0.52%, 0.92% with the insecticidal effect of chemical pesticide DDVP, substantially approach the insecticidal effect of chemical pesticide DDVP.

Embodiment 2:

While being chosen in larva before 3 ages of 3 kinds of organic water chestnuts primary pest or nymph service time, spray medicine, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and also can carry out early prevention. When use, the thin water layer that water 2～5cm is dark is stayed in field, uses filtrate 25kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in before at 10 in fine day morning or spray at dusk, spraying in rear guarantee 15～20h and do not rain, also manually not spraying, if rained in 15～20h or carried out artificial sprinkling irrigation, need again spray. Larva or nymph before 3 ages of spraying time are killed, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and droplet is as far as possible fine, not repeat spraying, be limited not form the globule on plant, safe defence will strictly be carried out human body defence and field operation by chemical pesticide operational procedure. After spray medicine, in 30d, the mixed battalion of breeding can not be carried out in field.

The quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. After 24h, the insecticidal effect in organic water chestnut field is: the white standing grain snout moth's larva of the water chestnut death rate 93.30%, the water chestnut borer death rate 87.30%, the water chestnut aphid death rate 98.17%, only differ respectively 0.76% ,-1.69% ,-0.64% with the insecticidal effect of chemical pesticide DDVP, basic identical with the insecticidal effect of chemical pesticide DDVP.

Embodiment 3:

While being chosen in larva before 3 ages of 3 kinds of organic water chestnuts primary pest or nymph service time, spray medicine, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and also can carry out early prevention. When use, the thin water layer that water 2～5cm is dark is stayed in field, uses filtrate 30kg.667m^-2Press 1.0:2.0 dilution spraying. Be chosen in before at 10 in fine day morning or spray at dusk, spraying in rear guarantee 15～20h and do not rain, also manually not spraying, if rained in 15～20h or carried out artificial sprinkling irrigation, need again spray. Larva or nymph before 3 ages of spraying time are killed, water chestnut borer will be sprayed medicine in the time that ovum is hatched, and droplet is as far as possible fine, not repeat spraying, be limited not form the globule on plant, safe defence will strictly be carried out human body defence and field operation by chemical pesticide operational procedure. After spray medicine, in 30d, the mixed battalion of breeding can not be carried out in field.

The quality weight ratio that the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people urinate four components is 2.5:1.5:2.0:3.5. Press 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilution spraying. After 24h, the insecticidal effect in organic water chestnut field is: the white standing grain snout moth's larva of the water chestnut death rate 95.18%, the water chestnut borer death rate 88.29%, the water chestnut aphid death rate 98.69%, only differ respectively-1.12% ,-2.68% ,-1.15% with the insecticidal effect of chemical pesticide DDVP, basic identical with the insecticidal effect of chemical pesticide DDVP.

Claims

1. organic water chestnut vegetable insecticide and a using method thereof, is characterized in that, described organic water chestnut vegetable insecticide is urinated brewed forming by the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine compatibility people.

2. the organic water chestnut vegetable insecticide of one according to claim 1, it is characterized in that, the compatibility weight mass ratio of the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of auxiliary agent thunder godvine and brewed medium people urine is 2.0～3.0:1.0～2.0:1.5～2.5:3.0～4.0.

3. according to the organic water chestnut vegetable insecticide of the one described in claim 1 and 2, it is characterized in that, the preferred weight mass ratio of the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine and people's urine is 2.5:1.5:2.0:3.5.

4. according to the organic water chestnut vegetable insecticide described in claims 1 to 3, it is characterized in that, the fresh cane of Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine are all by the weight Mass Calculation of fresh material, people's urine is by the weight Mass Calculation of 1～30d, wherein the fresh fleshy root skin of thunder godvine is auxiliary agent, and people's urine is brewed medium.

5. according to the organic water chestnut vegetable insecticide described in claim 2 to 4, it is characterized in that, the fresh cane of material Coriaria sinica, the fresh cane of building plum grass, the fresh fleshy root skin of thunder godvine, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, and soil, water body and fertilizer etc. also want heavy metal free to pollute.

6. according to the organic water chestnut vegetable insecticide described in claim 2 to 4, it is characterized in that, when use, the thin water layer that water 2～5cm is dark need be stayed in field, be typically chosen in before at 10 in fine day morning or spray at dusk, after spraying, to ensure not rainy in 15～20h or manually spray, if rained in 15～20h or carried out artificial sprinkling irrigation, need again spray.

7. using method according to claim 6, is characterized in that, selects larva or nymph before 3 ages to spray medicine, and water chestnut borer will be sprayed medicine in the time that ovum is hatched, by every 667m²The dosage that paddy field area 20～30kg is brewed dose is pressed 1.0:2.0 dilution spraying, droplet is as far as possible fine, not repeat spraying, is limited not form the globule on plant, can carry out effective killing action to the white standing grain snout moth's larva of organic water chestnut Tanaka's water chestnut, water chestnut borer and three kinds of primary pests of water chestnut aphid.